BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,566,218	C→G	5.8%	G122R (GGT→CGT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,218	0	C	G	5.8%	364.3 / 11.2	103	G122R (GGT→CGT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (50/47); new base G (2/4); total (52/51)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 105 total aligned reads displayed.

GTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAA  >  NC_000913/3566072‑3566366

gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCg                                                                                                                                                     <  2:625171/148‑1 (MQ=255)

 tCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGGCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGa                                                                                                                                                    >  2:212657/1‑148 (MQ=255)

    ggCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGc                                                                                                                                                 >  2:589870/1‑148 (MQ=255)

     gCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCCCCAGACGTCCCAGGCCACCGTTACCGAGGCCt                                                                                                                                               <  3:96522/149‑1 (MQ=255)

        aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTgg                                                                                                                                             <  2:190596/148‑1 (MQ=255)

        aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTgg                                                                                                                                             >  1:858609/1‑148 (MQ=255)

          tctTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTc                                                                                                                                          >  4:472297/1‑149 (MQ=255)

            tttCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTgggacgt                                                                                                                                        <  4:458129/149‑5 (MQ=255)

            tttCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                        >  4:394683/1‑149 (MQ=255)

             ttCTGGCTACCGTTAACGATGTTCTGCGTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAtt                                                                                                                                       >  3:802677/1‑149 (MQ=255)

                 ggCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   <  3:151646/149‑1 (MQ=255)

                   cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGGTGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                        >  1:557010/1‑142 (MQ=255)

                   cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGGTGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                        <  2:557010/142‑1 (MQ=255)

                   cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                                 <  3:412477/149‑1 (MQ=255)

                     aCCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcat                                                                                                                               <  3:394683/149‑1 (MQ=255)

                     aCCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtca                                                                                                                                >  1:802086/1‑148 (MQ=255)

                         ttAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaat                                                                                                                           >  3:110492/1‑149 (MQ=255)

                         ttAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaa                                                                                                                            <  2:802086/148‑1 (MQ=255)

                                  ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCtt                                                                                                                   <  1:295408/148‑1 (MQ=255)

                                  ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTc                                                                                                                  <  3:123810/149‑1 (MQ=255)

                                    cTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCg                                                                                                                 <  1:91748/148‑1 (MQ=255)

                                       cTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAt                                                                                                             >  4:640276/1‑149 (MQ=255)

                                        ttGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAt                                                                                                             >  2:316748/1‑148 (MQ=255)

                                         tGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAtt                                                                                                            >  1:62263/1‑148 (MQ=255)

                                            aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGGCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTaa                                                                                                         <  1:212657/148‑1 (MQ=255)

                                            aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAc                                                                                                        <  4:802677/149‑1 (MQ=255)

                                              aTACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAc                                                                                                        <  3:476985/147‑1 (MQ=255)

                                              aTACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAc                                                                                                        >  4:476985/1‑147 (MQ=255)

                                                  cGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACGCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCAt                                                                                                   >  2:691158/1‑148 (MQ=255)

                                                  cGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCAt                                                                                                   >  1:798807/1‑148 (MQ=255)

                                                     aGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGc                                                                                                >  2:686395/1‑148 (MQ=255)

                                                          tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAg                                                                                          >  3:88847/1‑149 (MQ=255)

                                                            gCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTAGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaat                                                                                                                           >  3:696634/1‑114 (MQ=255)

                                                            gCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTAGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaat                                                                                                                           <  4:696634/114‑1 (MQ=255)

                                                             cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTgc                                                                                       <  4:33079/149‑1 (MQ=255)

                                                                     gTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   <  1:616130/97‑1 (MQ=255)

                                                                     gTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   >  2:616130/1‑97 (MQ=255)

                                                                     gTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTa                                                                               >  3:906179/1‑149 (MQ=255)

                                                                         ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATc                                                                           <  3:350749/149‑1 (MQ=255)

                                                                           gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGTGTCATTttct                                                                                                                                   <  4:207754/91‑1 (MQ=255)

                                                                           gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   >  3:207754/1‑91 (MQ=255)

                                                                           gcgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCt                                                                          >  2:378478/1‑148 (MQ=255)

                                                                            cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCtt                                                                         >  2:265465/1‑148 (MQ=255)

                                                                            cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCtt                                                                         <  2:463964/148‑1 (MQ=255)

                                                                            cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCtt                                                                         <  1:20295/148‑1 (MQ=255)

                                                                             gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTc                                                                                             <  3:699194/127‑1 (MQ=255)

                                                                             gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTc                                                                                             >  4:699194/1‑127 (MQ=255)

                                                                                   gCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAtt                                                                 <  3:431351/149‑1 (MQ=255)

                                                                                   gCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAt                                                                  <  2:62263/148‑1 (MQ=255)

                                                                                    cAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAtt                                                                 <  1:589870/148‑1 (MQ=255)

                                                                                       cccTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCt                                                              <  1:867207/148‑1 (MQ=255)

                                                                                       cccTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTa                                                             <  3:54487/149‑1 (MQ=255)

                                                                                              gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaa                                                      >  3:736151/1‑149 (MQ=255)

                                                                                                 gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACAt                                                   >  3:205360/1‑149 (MQ=255)

                                                                                                    agagAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGc                                                >  3:183627/1‑149 (MQ=255)

                                                                                                      agaATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCg                                               <  1:378478/148‑1 (MQ=255)

                                                                                                       gaATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              <  1:498316/148‑1 (MQ=255)

                                                                                                          tCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTAc                                                                              >  3:494396/1‑113 (MQ=255)

                                                                                                          tCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTAc                                                                              <  4:494396/113‑1 (MQ=255)

                                                                                                          tCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                           <  2:773527/148‑1 (MQ=255)

                                                                                                            aaGGAAGCAAGCCGCCAGACGTCCCGGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAGCCCTAGCGACAACATGGCGTTGgaga                                        <  4:43629/149‑1 (MQ=255)

                                                                                                            aaGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgag                                         >  1:712725/1‑148 (MQ=255)

                                                                                                             aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgaga                                        >  1:126398/1‑148 (MQ=255)

                                                                                                              ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGagag                                       <  1:575674/148‑1 (MQ=255)

                                                                                                              ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGagag                                       >  1:856635/1‑148 (MQ=255)

                                                                                                              ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                                      >  4:656765/1‑149 (MQ=255)

                                                                                                               gAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                                      >  2:914651/1‑148 (MQ=255)

                                                                                                                 agcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTa                                   <  3:17933/149‑1 (MQ=255)

                                                                                                                 agcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTa                                   >  3:570121/1‑149 (MQ=255)

                                                                                                                      gccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAtt                                                                 <  2:397015/114‑1 (MQ=255)

                                                                                                                      gccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAtt                                                                 >  1:397015/1‑114 (MQ=255)

                                                                                                                       ccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGcc                              >  1:250476/1‑148 (MQ=255)

                                                                                                                       ccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGcc                              <  1:265465/148‑1 (MQ=255)

                                                                                                                          ccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                           >  1:117160/1‑148 (MQ=255)

                                                                                                                               cGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaaa                     <  4:335556/149‑1 (MQ=255)

                                                                                                                               cGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      <  1:316748/148‑1 (MQ=255)

                                                                                                                                gTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      >  4:900990/1‑147 (MQ=255)

                                                                                                                                gTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      <  3:900990/147‑1 (MQ=255)

                                                                                                                                gTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAAc                    >  4:139242/1‑149 (MQ=255)

                                                                                                                                 acccAGGCCTCGGTAACGGTGGCCTGGGACATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTGCATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              <  1:202006/121‑1 (MQ=38)

                                                                                                                                 acccAGGCCTCGGTAACGGTGGCCTGGGACATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTGCATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              >  2:202006/2‑122 (MQ=38)

                                                                                                                                   ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAACCTcc                 <  4:110492/149‑1 (MQ=255)

                                                                                                                                   ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                 >  3:491719/1‑149 (MQ=255)

                                                                                                                                   ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                 <  4:570121/149‑1 (MQ=255)

                                                                                                                                    cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                 >  2:62512/1‑148 (MQ=255)

                                                                                                                                     aGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgag                                         <  1:401756/123‑1 (MQ=255)

                                                                                                                                     aGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgag                                         >  2:401756/1‑123 (MQ=255)

                                                                                                                                     aGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa                <  2:798807/148‑1 (MQ=255)

                                                                                                                                      ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              <  1:359155/117‑1 (MQ=255)

                                                                                                                                      ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              >  2:359155/1‑117 (MQ=255)

                                                                                                                                      ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATc              <  4:88847/149‑1 (MQ=255)

                                                                                                                                       gCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg             >  4:594341/1‑149 (MQ=255)

                                                                                                                                          aCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      >  1:392618/1‑137 (MQ=255)

                                                                                                                                          aCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      <  2:392618/137‑1 (MQ=255)

                                                                                                                                          aCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa          >  4:120299/1‑149 (MQ=255)

                                                                                                                                           ccGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa          >  2:111244/1‑148 (MQ=255)

                                                                                                                                           ccGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAg         >  4:621452/1‑149 (MQ=255)

                                                                                                                                              ttACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACCGgt       <  2:340392/148‑1 (MQ=255)

                                                                                                                                                  cGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  >  4:46086/1‑149 (MQ=255)

                                                                                                                                                  cGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  >  4:143895/1‑149 (MQ=255)

GTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAA  >  NC_000913/3566072‑3566366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`