Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,689 G→T 7.0% intergenic (+40/+62) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6890GT 7.0% 242.1 / 5.1 81intergenic (+40/+62)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (36/38);  new base T (4/2);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAC  >  NC_000913/3608547‑3608835
                                                                                                                                               |                                                                                                                                                   
cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCct                                                                                                                                                >  2:588417/1‑148 (MQ=255)
 acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                              >  3:139364/1‑149 (MQ=255)
 acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctc                                                                                                                                               >  2:432853/1‑148 (MQ=255)
    cTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                           <  4:816949/149‑1 (MQ=255)
      gTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgggg                                                                                                                                          >  1:754217/1‑148 (MQ=255)
      gTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                         <  3:265978/149‑1 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                            >  3:130433/1‑145 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                            <  4:130433/145‑1 (MQ=255)
         aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                     <  3:580578/149‑1 (MQ=255)
          gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCTCCTCTGGGGAGAgg                                                                                                                                    <  3:67490/149‑1 (MQ=255)
          gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                     <  1:319121/148‑1 (MQ=255)
          gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                     <  2:226194/148‑1 (MQ=255)
            gATGACCGGGTTGTGGTTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                   >  1:580439/1‑148 (MQ=255)
            gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                  >  3:30833/1‑149 (MQ=255)
              tGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                 <  2:510813/148‑1 (MQ=255)
               gACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAggg                                                                                                                                   <  4:903360/145‑1 (MQ=255)
                aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                              >  4:112758/1‑149 (MQ=255)
                aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                              <  4:470791/149‑1 (MQ=255)
                  cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                             <  2:148691/148‑1 (MQ=255)
                  cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGt                                                                                                                            <  3:644412/149‑1 (MQ=255)
                   gggTTGTGGCTGGCAGTGCTGGCAGATGCGGGGGCGACGGTGCTGGTGAAAACGAATGCGATAAGATTGTTGCGCAGGAGATAAGGCAAACCGAGCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGACAggga                                                                                                                            >  2:689923/1‑147 (MQ=255)
                   gggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGt                                                                                                                            >  2:197909/1‑148 (MQ=255)
                     gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                         <  3:238879/149‑1 (MQ=255)
                     gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                         <  3:781725/149‑1 (MQ=255)
                     gTTGTGGCTGGCAGTGCTGGCACATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGCTAGGGTGa                                                                                                                          <  1:783568/148‑1 (MQ=255)
                      ttGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                        <  4:785094/149‑1 (MQ=255)
                      ttGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                        <  3:309811/149‑1 (MQ=255)
                            ctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                   <  1:805179/148‑1 (MQ=255)
                              ggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                              <  3:296398/135‑1 (MQ=255)
                              ggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                              >  4:296398/1‑135 (MQ=255)
                              ggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                >  3:224344/1‑149 (MQ=255)
                                     cTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATc                                                                                                         >  3:365480/1‑149 (MQ=255)
                                         cAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGaa                                                                                                      >  2:737947/1‑148 (MQ=255)
                                             taggggggCGAGGGTGCGGGTAACAGCGAAGGCGTAAAGATTGTTGCCACGGAGAAAAGGCAACCGGGCAGCAACATTCGGCGCGACCGGCCCCCCCGCCCCTCTGGGGGGGGGGTTCGGGTGAGGGAAAAAGGCGGCATCGAAGCCaa                                                                                                 <  4:365480/146‑1 (MQ=255)
                                                    gggagggtggtGGGGACAGCGAAGGGGTTAAGGTTGTTGCGAGGGAAATAAGGAAAACCAACCCAAACATGGAGGGCATACGTTCCCCTCGCCCCGCTGGGGAGAGGGTGGGGTTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                          <  4:895378/144‑1 (MQ=255)
                                                     cGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                          >  1:366333/1‑148 (MQ=255)
                                                      gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                        >  4:667718/1‑149 (MQ=255)
                                                        cGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                       >  1:648394/1‑148 (MQ=255)
                                                               ggTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTaagatcgg                                                                                >  1:664310/1‑141 (MQ=255)
                                                                gTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                               >  1:564041/1‑148 (MQ=255)
                                                                    cggaaaaTGGGTTAAGTTCTTTGCGGGGGGGAAAGGAAAACCAGATCACAAAATTGAGCGTGATCGGTCCCCTCGCCCCGCGGGGAGAAGGGTTGGGCGGGGAGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                          <  3:112758/144‑1 (MQ=255)
                                                                       caaaTGCTTTAAGATTGTGGCGAAGGAGAAAAGGAAAACCGATCGCAACATTGGGCGCGACCGCCCCCCCCCCCCTTGGGGGGAGGGGGTTGGGGTGGGGGAAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                        <  1:737947/146‑1 (MQ=255)
                                                                        gAATGCTTTAAGTTTGTGGCGCAGGAGAAAAGGAAACCCGACCGAAAAATGGAGCGCGATCGGCCCCCCCGCCCCTCGGGGGAGAGGGTTAGGGGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                       <  1:432853/148‑1 (MQ=255)
                                                                         aaTGCGTTAAGTTTGTTGCGAAGGAAATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGCCCCCCCGCCCCTCTGGGGAGGGGGTTAGGGTGGGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                     <  4:823348/149‑1 (MQ=255)
                                                                               ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                <  2:651202/100‑1 (MQ=255)
                                                                               ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                >  1:651202/1‑100 (MQ=255)
                                                                                    aTTTTTGCGAAGAAAATAAGGCAAACCGATCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGAt                                                          <  3:884661/149‑1 (MQ=38)
                                                                                        ttgCGCAGGAGATAAGGAAAACGGAGCGAAACATTGCGCGCGATCGGCCCCCCCGCCCCTCGGGGGAGGGGGTTGGGGGGGGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                      <  4:139364/149‑1 (MQ=255)
                                                                                        tcgggaaGGAGAAAAGAAAAACCAAACAAAATATGGCGCGCGAGCGCCCCCCCCGGCCCTCGGGGGGGGGGGTGAGGGTGAGGAGAAAAGGCGGCCTCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                       <  1:831349/142‑1 (MQ=255)
                                                                                            gcAGGAAAGAAGGAAAACCGATCGAAACATGGAGCGCGATCGGCCCCCTCGCCCCTCGGGGGAGAGGGTTGGGGTGGGGGAAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGt                                                  <  4:30833/149‑1 (MQ=255)
                                                                                              aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTc                                                 >  2:36134/1‑148 (MQ=255)
                                                                                                    tAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                          >  3:893964/1‑149 (MQ=255)
                                                                                                    tAAGGCAAACCGATCGCAACATTGAGCGCGATCGGCCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCATTTACGCATCTAATAACGATAAGGCAGTCCATCCGt                                          >  4:560097/1‑149 (MQ=39)
                                                                                                     aaGGCAAACCGATCGCAACATTGGGCGCGATCGGCCCCCTCGCCCTTCGGGGGAGAGGTTTAGGGGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                          <  1:60639/148‑1 (MQ=255)
                                                                                                         cAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTGGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                      <  2:754217/148‑1 (MQ=255)
                                                                                                           aaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                    >  1:458674/1‑148 (MQ=255)
                                                                                                           aaCCGATCGAAACATTGAGCGGGATCGGCCCCCCCGCCCCTCGGGGGGGGGGGTTAGGGGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                    <  2:366333/148‑1 (MQ=255)
                                                                                                            cccGATCAAAATATTGGGGGCGAGCGGCCCCCCGGCCCCTGTGGGGAGGGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                   <  2:315070/147‑1 (MQ=255)
                                                                                                                 aTCGCACAATGGAGCGCGATCGGCCCCCCCGCCCCTCGGGGGAGAGGGTTAGGGGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                              <  3:560097/149‑1 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt                          >  3:192878/1‑149 (MQ=255)
                                                                                                                       actttGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                         <  2:564041/145‑1 (MQ=255)
                                                                                                                         caatgttgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCg                       >  1:845521/8‑148 (MQ=255)
                                                                                                                             aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                   >  2:910059/1‑52 (MQ=255)
                                                                                                                             aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                   <  1:910059/52‑1 (MQ=255)
                                                                                                                              gcgcGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                 >  3:538243/1‑149 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGGGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa             >  4:479188/1‑149 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa             >  3:659032/1‑149 (MQ=255)
                                                                                                                                   aTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa             >  2:280899/1‑148 (MQ=255)
                                                                                                                                   aTCGGTCCCCTCGCCCCTCAGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAGTAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCACTTCGTGTTCCATaa             >  2:81827/1‑148 (MQ=255)
                                                                                                                                    gcGGTCCCCTCGCCCCCATGGGGAAGGGGTTAGGGATAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa            <  1:689923/147‑1 (MQ=255)
                                                                                                                                      ctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAgg          >  1:381634/5‑148 (MQ=255)
                                                                                                                                       taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt         >  2:379286/4‑148 (MQ=255)
                                                                                                                                       taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa        >  3:722052/4‑149 (MQ=255)
                                                                                                                                        tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa        >  1:908689/1‑148 (MQ=255)
                                                                                                                                         acccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc       >  2:587681/2‑148 (MQ=255)
                                                                                                                                          cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa            <  4:821723/142‑1 (MQ=255)
                                                                                                                                           ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                    >  3:75240/1‑101 (MQ=255)
                                                                                                                                           ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                    <  4:75240/101‑1 (MQ=255)
                                                                                                                                           ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaa     >  1:907364/1‑148 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa   <  3:667718/149‑1 (MQ=255)
                                                                                                                                              cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc  >  1:387282/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAC  >  NC_000913/3608547‑3608835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: