BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,636,747	T→C	6.7%	T222A (ACC→GCC)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,747	0	T	C	6.7%	390.6 / 12.9	120	T222A (ACC→GCC)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (60/52); new base C (5/3); total (65/55)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 120 total aligned reads displayed.

CATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAA  >  NC_000913/3636606‑3636892

cATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtt                                                                                                                                             <  2:20915/148‑1 (MQ=255)

 aTTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtttt                                                                                                                                           >  4:810195/1‑149 (MQ=255)

  ttCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtttt                                                                                                                                           >  1:149818/1‑148 (MQ=255)

  ttCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTc                                                                                                                                          >  3:386662/1‑149 (MQ=255)

   tCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTc                                                                                                                                          <  1:497756/148‑1 (MQ=255)

     aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGc                                                                                                                                       >  3:669052/1‑149 (MQ=255)

       gAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCgg                                                                                                                                     <  4:776293/149‑1 (MQ=255)

        aaGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGt                                                                                                                                    <  3:195594/149‑1 (MQ=255)

          ggTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTaa                                                                                                                                  <  3:768838/149‑1 (MQ=255)

            tttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                                >  3:274458/1‑149 (MQ=255)

             ttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGc                                                                                                                                                 >  1:22195/1‑131 (MQ=255)

             ttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGc                                                                                                                                                 <  2:22195/131‑1 (MQ=255)

             ttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                                >  2:105274/1‑148 (MQ=255)

               cGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCAc                                                                                                                              <  1:694475/148‑1 (MQ=255)

                 aGGTCCACATCCGGTAGCAGATTGATGCTGACAAAGTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACgg                                                                                                                            >  2:557921/1‑148 (MQ=255)

                  ggTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGa                                                                                                                           <  1:330424/148‑1 (MQ=255)

                  ggTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGa                                                                                                                           >  1:753586/1‑148 (MQ=255)

                   gTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAg                                                                                                                         >  4:60964/1‑149 (MQ=255)

                      cacaTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCAGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                      >  4:512964/1‑149 (MQ=255)

                           ccGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGc                                                                                                                  >  1:608130/1‑148 (MQ=255)

                                 gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcc                                                                                                            <  1:63190/148‑1 (MQ=255)

                                    gATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcccgc                                                                                                         >  1:832360/1‑148 (MQ=255)

                                    gATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcccgc                                                                                                         >  1:434726/1‑148 (MQ=255)

                                        gATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCa                                                                                                    <  3:680602/149‑1 (MQ=255)

                                         aTGGTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCAc                                                                                                   <  3:540230/149‑1 (MQ=255)

                                                  aaTTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAAct                                                                                           >  1:636931/1‑148 (MQ=255)

                                                  aaTTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAAct                                                                                           >  1:398565/1‑148 (MQ=255)

                                                       ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                      >  2:124064/1‑148 (MQ=255)

                                                       ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                      <  1:563204/148‑1 (MQ=255)

                                                        cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCAc                                                                                                                     <  2:282040/116‑1 (MQ=255)

                                                        cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCAc                                                                                                                     >  1:282040/1‑116 (MQ=255)

                                                        cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                     >  2:284256/1‑148 (MQ=255)

                                                        cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAg                                                                                    >  3:4789/1‑149 (MQ=255)

                                                        cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAg                                                                                    >  3:794777/1‑149 (MQ=255)

                                                         ccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAg                                                                                    >  1:108795/1‑148 (MQ=255)

                                                            gTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCaa                                                                                 <  2:522849/148‑1 (MQ=255)

                                                               gCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACgg                                                                              <  2:623713/148‑1 (MQ=255)

                                                                 cAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                           >  3:30020/1‑149 (MQ=255)

                                                                  aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGa                                                                                                                           >  2:21442/1‑100 (MQ=255)

                                                                  aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGa                                                                                                                           <  1:21442/100‑1 (MQ=255)

                                                                  aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCa                                                                                                    >  4:636354/1‑123 (MQ=255)

                                                                  aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                           >  2:561945/1‑148 (MQ=255)

                                                                        ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                    >  4:712871/1‑149 (MQ=255)

                                                                         tGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                    >  1:431500/1‑148 (MQ=255)

                                                                         tGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                    <  2:747129/148‑1 (MQ=255)

                                                                         tGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTc                                                                                                                                          <  1:860998/78‑1 (MQ=255)

                                                                          gAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGaa                                                                                                                          >  4:461570/1‑93 (MQ=255)

                                                                          gAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGaa                                                                                                                          <  3:461570/93‑1 (MQ=255)

                                                                            aaTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCa                                                                                  >  2:303182/1‑131 (MQ=255)

                                                                            aaTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCa                                                                                  <  1:303182/131‑1 (MQ=255)

                                                                             aTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                               <  3:106450/149‑1 (MQ=255)

                                                                             aTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                               >  3:315055/1‑149 (MQ=255)

                                                                             aTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                               <  4:4789/149‑1 (MQ=255)

                                                                                   aaCACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAc                                                         <  3:710244/149‑1 (MQ=255)

                                                                                      acCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCa                                                       >  2:258104/1‑148 (MQ=255)

                                                                                         gccgGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGGATGGCACCAGAcc                                                   >  3:819457/1‑149 (MQ=255)

                                                                                          ccgGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAcc                                                   >  1:452768/1‑148 (MQ=255)

                                                                                              tCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgc                                              >  3:803866/1‑149 (MQ=255)

                                                                                               ccAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgc                                              <  2:452768/148‑1 (MQ=255)

                                                                                                cAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                             >  1:300539/1‑148 (MQ=255)

                                                                                                 aGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAAGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCggg                                           >  4:282558/1‑149 (MQ=255)

                                                                                                  gACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCggg                                           >  1:669496/1‑148 (MQ=255)

                                                                                                   aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                         >  3:156553/1‑101 (MQ=255)

                                                                                                   aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGCAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                         <  4:156553/101‑1 (MQ=255)

                                                                                                      aGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATg                                                                        <  1:874025/115‑1 (MQ=255)

                                                                                                      aGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATg                                                                        >  2:874025/1‑115 (MQ=255)

                                                                                                       gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                     <  3:810195/149‑5 (MQ=255)

                                                                                                       gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctgg                                      <  1:879318/148‑4 (MQ=255)

                                                                                                       gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgca                                     <  3:886064/149‑1 (MQ=255)

                                                                                                              tgggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                              >  4:360012/1‑149 (MQ=255)

                                                                                                                ggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                            <  4:794777/149‑1 (MQ=255)

                                                                                                                 gtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                            <  2:832360/148‑1 (MQ=255)

                                                                                                                 gtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                            <  2:218714/148‑1 (MQ=255)

                                                                                                                  tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAgg                          >  4:883735/1‑149 (MQ=255)

                                                                                                                  tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAg                           >  3:232260/1‑148 (MQ=255)

                                                                                                                  tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAg                           <  4:232260/148‑1 (MQ=255)

                                                                                                                      gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                      >  4:655078/1‑149 (MQ=255)

                                                                                                                         tAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGa                                                                >  1:601246/1‑104 (MQ=255)

                                                                                                                         tAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGa                                                                <  2:601246/104‑1 (MQ=255)

                                                                                                                         tAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAAtt                   >  4:144024/1‑149 (MQ=255)

                                                                                                                             ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                     <  3:679055/127‑5 (MQ=255)

                                                                                                                             ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                     >  4:679055/1‑123 (MQ=255)

                                                                                                                             ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                <  2:108795/148‑1 (MQ=255)

                                                                                                                             ttCTCACGGAAAACGGTGCCGTGTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGTCCAAATTGTTc               <  3:512964/149‑1 (MQ=255)

                                                                                                                              tctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctgg                                      <  1:420833/125‑4 (MQ=255)

                                                                                                                              tctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctgg                                      >  2:420833/1‑122 (MQ=255)

                                                                                                                              tctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc               >  1:787503/1‑148 (MQ=255)

                                                                                                                                tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAAt                    <  3:319336/141‑1 (MQ=255)

                                                                                                                                tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAAt                    >  4:319336/1‑141 (MQ=255)

                                                                                                                                tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc            <  3:655078/149‑1 (MQ=255)

                                                                                                                                 cACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc            >  2:338861/1‑148 (MQ=255)

                                                                                                                                   ctgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                <  1:653292/140‑1 (MQ=255)

                                                                                                                                   ctgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                >  2:653292/3‑142 (MQ=255)

                                                                                                                                     gAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc        >  1:674303/1‑148 (MQ=255)

                                                                                                                                     gAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc        >  2:712376/1‑148 (MQ=255)

                                                                                                                                     gAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc        >  1:520649/1‑148 (MQ=255)

                                                                                                                                       aaaCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCtt      <  1:274959/148‑1 (MQ=255)

                                                                                                                                        aaCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGACAGCACGTTGAGGCCAAATTGTTCGGCAATCTta     <  1:124064/148‑1 (MQ=255)

                                                                                                                                         aCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTtata   >  3:99123/1‑149 (MQ=255)

                                                                                                                                          cGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATaa  <  3:360012/149‑1 (MQ=255)

CATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAA  >  NC_000913/3636606‑3636892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`