Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,716,509 C→T 7.6% intergenic (‑115/‑38) bisC ← / → yiaD biotin sulfoxide reductase/multicopy suppressor of bamB; outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,716,5090CT 7.6% 242.9 / 7.5 79intergenic (‑115/‑38)bisC/yiaDbiotin sulfoxide reductase/multicopy suppressor of bamB; outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (34/39);  new base T (3/3);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCT  >  NC_000913/3716364‑3716651
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gAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCg                                                                                                                                             >  3:402829/1‑149 (MQ=255)
  aCGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGt                                                                                                                                            >  2:498406/1‑148 (MQ=255)
      aaTATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCa                                                                                                                                        >  1:867774/1‑148 (MQ=255)
       atatCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCaaa                                                                                                                                      <  3:417037/149‑1 (MQ=255)
        tatCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAACTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCaaa                                                                                                                                      <  1:198670/148‑1 (MQ=255)
           cGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGcg                                                                                                                                        <  1:195917/143‑2 (MQ=255)
           cGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGcg                                                                                                                                        >  2:195917/1‑142 (MQ=255)
             tGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATa                                                                                                                                 >  1:172656/1‑148 (MQ=255)
              gAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGATTAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATaa                                                                                                                                >  2:104094/1‑148 (MQ=255)
                ggATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATaaaaa                                                                                                                             <  4:397975/149‑1 (MQ=255)
                 gATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCg                                                                                                                                             >  1:122323/1‑132 (MQ=255)
                 gATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCg                                                                                                                                             <  2:122323/132‑1 (MQ=255)
                 gATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATaaaaa                                                                                                                             <  2:314242/148‑1 (MQ=255)
                  aTGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAg                                                                                                                            >  1:564161/1‑148 (MQ=255)
                      gTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAAcc                                                                                                                                               <  3:146184/125‑1 (MQ=255)
                      gTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAAcc                                                                                                                                               >  4:146184/1‑125 (MQ=255)
                        tGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGc                                                                                                                                         <  2:792479/129‑1 (MQ=255)
                        tGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGc                                                                                                                                         >  1:792479/1‑129 (MQ=255)
                        tGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttatt                                                                                                                      >  1:648683/1‑148 (MQ=255)
                         ggCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttatta                                                                                                                     <  2:867774/148‑1 (MQ=255)
                         ggCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttatta                                                                                                                     <  1:818203/148‑1 (MQ=255)
                            cAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTaaag                                                                                                                  >  2:401892/1‑146 (MQ=255)
                                ccGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTaa                                                                                                             >  3:108656/1‑149 (MQ=255)
                                 cGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                            <  4:761233/149‑1 (MQ=255)
                                  gAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                            >  1:594678/1‑148 (MQ=255)
                                    aGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAAt                                                                                                         <  4:236624/149‑1 (MQ=255)
                                       cTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATga                                                                                                       <  2:172656/148‑1 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCTGCCACGGGTTCCGTGGcgggag                                                                                                                                    <  1:357749/117‑6 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCTGCCACGGGTTCCGTGGcgggag                                                                                                                                    >  2:357749/1‑112 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACa                                                                                                           >  1:422795/1‑142 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACa                                                                                                           <  2:422795/142‑1 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaag                                                                                                     <  1:24587/148‑1 (MQ=255)
                                           tGATAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAGTAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                            >  4:572816/1‑139 (MQ=255)
                                           tGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGa                                                                                                                 >  2:721804/1‑134 (MQ=255)
                                           tGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGa                                                                                                                 <  1:721804/134‑1 (MQ=255)
                                           tGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGaaa                                                                                                  >  4:746910/1‑149 (MQ=255)
                                           tGAAAGCTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                            <  3:572816/139‑1 (MQ=255)
                                            gAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGaaa                                                                                                  >  2:656865/1‑148 (MQ=255)
                                            gAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAAc                                                                                                 <  4:402829/149‑1 (MQ=255)
                                              aaGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATga                                                                                                       <  2:66090/141‑1 (MQ=255)
                                              aaGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATga                                                                                                       >  1:66090/1‑141 (MQ=255)
                                                ggTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGGAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACgtg                                                                                              <  2:248869/148‑1 (MQ=255)
                                                   tAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGttt                                                                                           >  1:541945/1‑148 (MQ=255)
                                                    aGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTa                                                                                          <  2:594678/148‑1 (MQ=255)
                                                         tATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGa                                                                                                                 <  1:717174/120‑1 (MQ=255)
                                                         tATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGa                                                                                                                 >  2:717174/1‑120 (MQ=255)
                                                                cAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGaaa                                                                                                  >  2:233798/1‑128 (MQ=255)
                                                                cAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGaaa                                                                                                  <  1:233798/128‑1 (MQ=255)
                                                                  gTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGccgccg                                                                            <  1:401892/148‑1 (MQ=255)
                                                                   ttAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATATTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTa                                                                          <  3:746910/149‑1 (MQ=255)
                                                                    tAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTa                                                                          >  2:739381/1‑148 (MQ=255)
                                                                        tatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgag                                                                     >  4:66214/1‑149 (MQ=255)
                                                                          ttacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgagt                                                                    <  1:93474/148‑1 (MQ=255)
                                                                               tacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTa                                                                          >  2:205282/1‑137 (MQ=255)
                                                                               tacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTa                                                                          <  1:205282/137‑1 (MQ=255)
                                                                                  aaaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTaa                                                                                                             >  3:291726/1‑99 (MQ=255)
                                                                                  aaaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTaa                                                                                                             <  4:291726/99‑1 (MQ=255)
                                                                                   aaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAgg                                                                                                                  <  1:414323/93‑1 (MQ=255)
                                                                                   aaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAgg                                                                                                                  >  2:414323/1‑93 (MQ=255)
                                                                                   aaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTAtt                                                                                   >  4:522832/1‑124 (MQ=255)
                                                                                   aaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTAtt                                                                                   <  3:522832/124‑1 (MQ=255)
                                                                                    aTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTgg                                                          <  2:333326/148‑1 (MQ=255)
                                                                                          gAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATc                                                   >  4:204110/1‑149 (MQ=255)
                                                                                           aGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATc                                                   >  2:108692/1‑148 (MQ=255)
                                                                                               ttCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGAGGTATCTGGCt                                              <  3:781856/149‑1 (MQ=255)
                                                                                                   cAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGcac                                          <  4:450622/149‑1 (MQ=255)
                                                                                                   cAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGcac                                          >  3:734047/1‑149 (MQ=255)
                                                                                                   cAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGca                                           >  1:519971/1‑148 (MQ=255)
                                                                                                     ggAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGcaca                                         <  1:67594/148‑1 (MQ=255)
                                                                                                           atCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAcc                                  <  4:108656/149‑1 (MQ=255)
                                                                                                              ttCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAc                                   <  1:141875/145‑1 (MQ=255)
                                                                                                              ttCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAc                                   >  2:141875/1‑145 (MQ=255)
                                                                                                                   gTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACAcc                          >  4:878403/1‑149 (MQ=255)
                                                                                                                    ttCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCg                         >  3:828420/1‑149 (MQ=255)
                                                                                                                         gcgGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGa                     <  1:656865/148‑1 (MQ=255)
                                                                                                                           ggCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAAcg                  <  3:494911/149‑1 (MQ=255)
                                                                                                                               tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGa               <  1:812072/148‑1 (MQ=255)
                                                                                                                                  tctcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGc            <  2:519971/148‑1 (MQ=255)
                                                                                                                                     cccGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAccc                                 >  2:546942/1‑124 (MQ=255)
                                                                                                                                     cccGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAccc                                 <  1:546942/124‑1 (MQ=255)
                                                                                                                                           aCGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCt  <  3:204110/149‑1 (MQ=255)
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GAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCT  >  NC_000913/3716364‑3716651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: