BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,778,879	T→C	5.6%	V494A (GTC→GCC)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,778,879	0	T	C	5.6%	1439.6 / 45.7	424	V494A (GTC→GCC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (176/223); new base C (9/15); total (186/238)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 428 total aligned reads displayed.

TCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAGGC  >  NC_000913/3778732‑3779026

tctcGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGt                                                                                                                                                     >  1:114372/1‑148 (MQ=255)

    gCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCg                                                                                                                                                >  3:46828/1‑149 (MQ=255)

     ccGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCg                                                                                                                                                >  2:721745/1‑148 (MQ=255)

      cGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGt                                                                                                                                              <  3:161706/149‑1 (MQ=255)

       gTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGtg                                                                                                                                             <  3:619762/149‑2 (MQ=255)

       gTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGtg                                                                                                                                             <  3:282006/149‑2 (MQ=255)

       gTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGt                                                                                                                                              <  1:172595/148‑1 (MQ=255)

        ttCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGtg                                                                                                                                             <  2:311562/148‑2 (MQ=255)

          ccTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAg                                                                                                                                           <  1:414202/148‑1 (MQ=255)

           cTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAga                                                                                                                                          <  2:573001/148‑1 (MQ=255)

            tCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgat                                                                                                                                         >  2:734265/1‑148 (MQ=255)

            tCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGACACCGGTAAgatg                                                                                                                                        >  4:571201/1‑149 (MQ=255)

              ggctggctGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatgat                                                                                                                                      <  4:746218/149‑1 (MQ=255)

              ggctggctGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatga                                                                                                                                       <  2:858254/148‑1 (MQ=255)

              ggctggctGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatga                                                                                                                                       <  1:611473/148‑1 (MQ=255)

                    ctggGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTcccc                                                                                                                                <  4:21726/149‑1 (MQ=255)

                      gggggTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCg                                                                                                                               >  2:380165/1‑148 (MQ=255)

                       ggggTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCa                                                                                                                             >  4:236606/1‑149 (MQ=255)

                        gggTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCaa                                                                                                                            <  3:766212/149‑1 (MQ=255)

                         ggTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCaa                                                                                                                            <  2:749921/148‑1 (MQ=255)

                           tATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTa                                                                                                                                             <  2:458841/129‑1 (MQ=255)

                            aTTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACAGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATcta                                                                                                                        <  4:818327/149‑1 (MQ=255)

                               ccTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatc                                                                                                                      <  1:341096/148‑1 (MQ=255)

                                cTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatcgc                                                                                                                    <  3:402804/149‑1 (MQ=255)

                                    ccGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatga                                                                                                                                       <  2:137288/126‑1 (MQ=255)

                                    ccGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatcgctatc                                                                                                                <  3:857777/149‑1 (MQ=255)

                                       ggTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGcc                                                                                                             <  4:457837/149‑1 (MQ=255)

                                            gATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCtgtg                                                                                                         <  2:120656/148‑1 (MQ=255)

                                            gATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTgc                                                                                                        >  3:244033/1‑149 (MQ=255)

                                            gATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCATCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTgc                                                                                                        >  4:783794/1‑149 (MQ=255)

                                             aTACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTgc                                                                                                        >  2:294169/1‑148 (MQ=255)

                                                 cTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGcggcgg                                                                                                   <  3:900151/149‑1 (MQ=255)

                                                   cATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGt                                                                                                  >  1:229078/1‑148 (MQ=255)

                                                    aTCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAg                                                                                                >  3:312175/1‑149 (MQ=255)

                                                     tCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAgg                                                                                               >  3:695538/1‑149 (MQ=255)

                                                      cTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAgg                                                                                               <  1:700581/148‑1 (MQ=255)

                                                       tAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGcc                                                                                             <  3:823498/149‑1 (MQ=255)

                                                        aaCGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGcc                                                                                             <  1:304465/148‑1 (MQ=255)

                                                         aCGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGTTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCt                                                                                            >  1:318523/1‑148 (MQ=255)

                                                           gCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGTCTGTGCGGCGGTAGGCCtgg                                                                                          <  2:174664/148‑1 (MQ=255)

                                                            cccTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggtg                                                                                        <  3:9899/149‑1 (MQ=255)

                                                             ccTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggtgg                                                                                       >  3:146795/1‑149 (MQ=255)

                                                              cTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTggg                                                                                      >  4:307095/1‑149 (MQ=255)

                                                                  tCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCaaa                                                                                  <  4:537559/149‑1 (MQ=255)

                                                                  tCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCaa                                                                                   <  1:59159/148‑1 (MQ=255)

                                                                    gccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAaga                                                                                >  3:354294/1‑149 (MQ=255)

                                                                    gccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAag                                                                                 <  2:330017/148‑1 (MQ=255)

                                                                     ccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAaga                                                                                >  1:683248/1‑148 (MQ=255)

                                                                            gCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGAtt                                                                        <  4:357416/149‑1 (MQ=255)

                                                                               aGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTg                                                                      >  2:642508/1‑148 (MQ=255)

                                                                                gCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGAt                                                                         <  2:426392/144‑1 (MQ=255)

                                                                                 ccACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGtt                                                                    <  1:281985/148‑1 (MQ=255)

                                                                                      gcagcaCAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCtt                                                              <  4:375194/149‑1 (MQ=255)

                                                                                        agcaCAACATATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCttt                                                             <  2:684967/148‑1 (MQ=255)

                                                                                        agcaCAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTa                                                            <  3:664997/149‑1 (MQ=255)

                                                                                         gcaCAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTa                                                            <  1:245233/148‑1 (MQ=255)

                                                                                          cacaACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTAc                                                           >  1:544911/1‑148 (MQ=255)

                                                                                              acaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTc                                                       >  1:465419/1‑148 (MQ=255)

                                                                                              acaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCAGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTGGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTc                                                       >  1:744347/1‑148 (MQ=255)

                                                                                               caaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGATTCTGATTTGTTCCGCTTTACTGTCa                                                      >  2:450204/1‑148 (MQ=255)

                                                                                                    tGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAacac                                                 <  2:61625/148‑1 (MQ=255)

                                                                                                    tGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAAcaca                                                <  3:296643/149‑1 (MQ=255)

                                                                                                      gCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAg                                               <  1:505254/148‑1 (MQ=255)

                                                                                                       cGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGc                                              <  1:810450/148‑1 (MQ=255)

                                                                                                          ctctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCAACGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGa                                          <  3:802583/149‑1 (MQ=255)

                                                                                                           tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACCGCCTGa                                          <  2:497516/148‑1 (MQ=255)

                                                                                                           tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGa                                          <  1:914285/148‑1 (MQ=255)

                                                                                                           tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                         >  3:131823/1‑149 (MQ=255)

                                                                                                             tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCt                                       >  4:423659/1‑149 (MQ=255)

                                                                                                             tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCt                                       >  4:643314/1‑149 (MQ=255)

                                                                                                              gATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGt                                                                     <  1:453366/118‑1 (MQ=255)

                                                                                                              gATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCtt                                      >  4:708907/1‑149 (MQ=255)

                                                                                                              gATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCt                                       >  1:654870/1‑148 (MQ=255)

                                                                                                                tCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTc                                     >  1:126065/1‑148 (MQ=255)

                                                                                                                tCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCa                                    <  3:750570/149‑1 (MQ=255)

                                                                                                                 cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCa                                    <  2:544911/148‑1 (MQ=255)

                                                                                                                 cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCAc                                   >  4:868446/1‑149 (MQ=255)

                                                                                                                 cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCAc                                   <  4:695538/149‑1 (MQ=255)

                                                                                                                 cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCAc                                   <  4:181695/149‑1 (MQ=255)

                                                                                                                  tGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCAcc                                  >  3:730876/1‑149 (MQ=255)

                                                                                                                     tGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTg                                >  1:637840/1‑148 (MQ=255)

                                                                                                                        tGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGt                                                                     >  3:517966/1‑108 (MQ=255)

                                                                                                                          gTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggtgg                                                                                       >  4:289989/1‑88 (MQ=255)

                                                                                                                                gccAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTcc                                                                  >  4:137213/1‑103 (MQ=255)

                                                                                                                                gccAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGt                    >  3:567433/1‑149 (MQ=255)

                                                                                                                                 ccAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGt                    >  1:905365/1‑148 (MQ=255)

                                                                                                                                  tcatcttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGa                  >  4:505332/8‑149 (MQ=255)

                                                                                                                                  cAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGtat                             >  2:210290/1‑138 (MQ=255)

                                                                                                                                    atcttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTAACGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGAt                 >  2:773285/6‑148 (MQ=255)

                                                                                                                                    aTACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGAt                 >  1:130595/1‑148 (MQ=255)

                                                                                                                                      accaccGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcac              <  3:219735/149‑1 (MQ=255)

                                                                                                                                      accaccGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATca               <  1:385725/148‑1 (MQ=255)

                                                                                                                                       ttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcacc             >  4:324323/3‑149 (MQ=255)

                                                                                                                                        caccGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcacc             <  2:905365/148‑1 (MQ=255)

                                                                                                                                         accGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCt                                                               <  2:246557/97‑1 (MQ=255)

                                                                                                                                         accGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                         <  1:880373/119‑1 (MQ=255)

                                                                                                                                         accGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                         >  2:880373/1‑119 (MQ=255)

                                                                                                                                         accGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcacca            >  2:513716/1‑148 (MQ=255)

                                                                                                                                           cGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACg          <  1:734265/148‑1 (MQ=255)

                                                                                                                                                 cGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAg    >  2:600977/1‑148 (MQ=255)

TCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAGGC  >  NC_000913/3778732‑3779026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`