Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,877,558 G→C 27.0% intergenic (‑93/+147) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,877,5580GC27.0% 70.9 / 29.0 37intergenic (‑93/+147)yidB/gyrBDUF937 family protein/DNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (14/13);  new base C (6/4);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGC  >  NC_000913/3877410‑3877702
                                                                                                                                                    |                                                                                                                                                
tatTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatg                                                                                                                                                  <  3:311374/149‑1 (MQ=255)
tatTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatg                                                                                                                                                  >  4:311374/1‑149 (MQ=255)
  tttCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                <  4:129153/149‑1 (MQ=255)
   ttCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtc                                                                                                                                               <  3:210100/149‑1 (MQ=255)
         gCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCCAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGcc                                                                                                                                         <  4:611757/149‑1 (MQ=255)
                cTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc                                                                                                                                          >  2:425228/1‑141 (MQ=255)
                cTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc                                                                                                                                          <  1:425228/141‑1 (MQ=255)
                                          tcaAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                <  4:571403/109‑1 (MQ=255)
                                          tcaAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                >  3:571403/1‑109 (MQ=255)
                                              aaaGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatga                                                                                                                                                 >  4:627969/1‑104 (MQ=255)
                                              aaaGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatga                                                                                                                                                 <  3:627969/104‑1 (MQ=255)
                                                     cATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTACAGCTTCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                              >  2:798548/1‑148 (MQ=255)
                                                      aTGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                            >  4:883575/1‑149 (MQ=255)
                                                        gATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGc                                                                                                                                          <  2:390072/101‑1 (MQ=255)
                                                        gATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGc                                                                                                                                          >  1:390072/1‑101 (MQ=255)
                                                                  tGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAt                                                                                >  3:607429/1‑149 (MQ=255)
                                                                     aaaGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCa                                                                              >  2:519999/1‑148 (MQ=255)
                                                                               gtgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTACAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCa                                                                    <  1:798548/148‑1 (MQ=255)
                                                                                 gtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATa                                                                 >  3:515867/1‑149 (MQ=255)
                                                                                  ttGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATa                                                                 >  2:89848/1‑148 (MQ=255)
                                                                                   tGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCa                                                                    <  1:840880/144‑1 (MQ=255)
                                                                                   tGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCa                                                                    >  2:840880/1‑144 (MQ=255)
                                                                                     aCCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                             <  4:904322/117‑1 (MQ=255)
                                                                                     aCCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                             >  3:904322/1‑117 (MQ=255)
                                                                                       ccAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAAt                                                                       >  3:170453/1‑137 (MQ=255)
                                                                                       ccAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAAt                                                                       <  4:170453/137‑1 (MQ=255)
                                                                                         aaaaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaaca                                                                        <  4:650053/134‑3 (MQ=255)
                                                                                         aaaaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaaaa                                                                        >  3:650053/1‑134 (MQ=255)
                                                                                           aaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACAt                                                              <  1:665436/142‑1 (MQ=255)
                                                                                           aaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACAt                                                              >  2:665436/1‑142 (MQ=255)
                                                                                                  aGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                 >  2:736295/1‑148 (MQ=255)
                                                                                                     tttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAAGGCCTTATCCGGCCTACAAAATCATGaa                                                                          <  3:603193/120‑1 (MQ=255)
                                                                                                     tttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaa                                                                          >  4:603193/1‑120 (MQ=255)
                                                                                                              tgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATaa                                     <  1:519999/148‑1 (MQ=255)
                                                                                                                  aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGt                                 <  1:89848/148‑1 (MQ=255)
                                                                                                                  aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTa                                <  3:883575/149‑1 (MQ=255)
                                                                                                                           ttGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAg                       <  4:515867/149‑1 (MQ=255)
                                                                                                                                          gggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt        >  3:310193/1‑149 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTc               <  4:12223/141‑1 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTc               >  3:12223/1‑141 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGtt       >  4:889945/1‑149 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGtt       >  3:227766/1‑149 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt        >  2:351948/1‑148 (MQ=255)
                                                                                                                                                agaTCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgcgc  <  3:889945/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGC  >  NC_000913/3877410‑3877702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑