Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,009,002 G→T 5.9% P20T (CCA→ACA)  rhtB ← homoserine, homoserine lactone and S‑methyl‑methionine efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,009,0020GT 5.9% 361.3 / 9.0 102P20T (CCA→ACA) rhtBhomoserine, homoserine lactone and S‑methyl‑methionine efflux pump
Reads supporting (aligned to +/- strand):  ref base G (47/49);  new base T (3/3);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 103 total aligned reads displayed.

ACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGT  >  NC_000913/4008854‑4009147
                                                                                                                                                    |                                                                                                                                                 
aCTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTgg                                                                                                                                                   >  3:641024/1‑149 (MQ=255)
        ggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAgg                                                                                                                                            >  2:718645/1‑148 (MQ=255)
        ggAAAATAGCGTCACCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCTCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACCGGc                                                                                                                                           <  4:771083/149‑1 (MQ=255)
          aaaaTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtc                                                                                                                                         <  4:26433/149‑2 (MQ=255)
                gCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTTATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAAt                                                                                                                                    <  2:887049/148‑1 (MQ=255)
                 cGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGa                                                                                                                                  <  3:625774/149‑1 (MQ=255)
                  gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGa                                                                                                                                  <  1:11249/148‑1 (MQ=255)
                  gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  3:308546/149‑1 (MQ=255)
                  gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGCTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGTCTTAAAATGa                                                                                                                                  <  1:743035/148‑1 (MQ=255)
                   tCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  1:752635/148‑1 (MQ=255)
                   tCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                <  4:23131/149‑1 (MQ=255)
                    ccccAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                >  1:825036/1‑148 (MQ=255)
                      ccAACCCCCCGCCAACCCGCACAATATGAATCGCCAGTCCGGTCTAAAGCACAGCAATAGACGTCACCGCGCCGCGATAACCGTGGTTCAGCGATGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAACTGATCGAt                                                                                                                             <  4:322812/149‑1 (MQ=255)
                       cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTaaa                                                                                                                                      >  1:517980/1‑139 (MQ=255)
                       cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTaaa                                                                                                                                      <  2:517980/139‑1 (MQ=255)
                       cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGAt                                                                                                                             >  1:463340/1‑148 (MQ=255)
                        aaCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGt                                                                                                                           <  3:483480/149‑1 (MQ=255)
                            ccACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTcag                                                                                                                        <  2:259315/148‑1 (MQ=255)
                              aCGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTcagca                                                                                                                      >  2:596144/1‑148 (MQ=255)
                                 ccAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   >  2:622815/1‑148 (MQ=255)
                                 ccAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   <  1:768548/148‑1 (MQ=255)
                                  cAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  2:478308/148‑1 (MQ=255)
                                   aaCCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAgg                                                                                                                >  3:543682/1‑149 (MQ=255)
                                        gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  3:818553/127‑1 (MQ=255)
                                        gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 >  4:818553/1‑127 (MQ=255)
                                        gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAac                                                                                                           <  4:338570/149‑1 (MQ=255)
                                          acaATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACa                                                                                                                                              >  1:893615/1‑112 (MQ=255)
                                          acaATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACa                                                                                                                                              <  2:893615/112‑1 (MQ=255)
                                            aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtctg                                                                                                                                       <  4:798828/117‑4 (MQ=255)
                                            aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtctg                                                                                                                                       >  3:798828/1‑114 (MQ=255)
                                            aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAaccacc                                                                                                       >  4:31560/1‑149 (MQ=255)
                                               atGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCAt                                                                                                     <  1:115213/148‑1 (MQ=255)
                                               atGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCAt                                                                                                     >  1:445205/1‑148 (MQ=255)
                                                   aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTaa                                                                                                <  3:31560/149‑1 (MQ=255)
                                                   aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTa                                                                                                 <  2:677341/148‑1 (MQ=255)
                                                    tCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTaa                                                                                                >  2:352876/1‑148 (MQ=255)
                                                    tCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAg                                                                                               <  4:591094/149‑1 (MQ=255)
                                                     cGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  >  2:410477/1‑129 (MQ=255)
                                                     cGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  1:410477/129‑1 (MQ=255)
                                                       ccAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTc                                                                                            >  4:343980/1‑149 (MQ=255)
                                                       ccAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTc                                                                                            >  3:266055/1‑149 (MQ=255)
                                                           tCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCatg                                                                                         <  1:352876/148‑1 (MQ=255)
                                                                tCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGaa                                                                                    <  1:718645/148‑1 (MQ=255)
                                                                  tGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   >  4:876138/1‑115 (MQ=255)
                                                                  tGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   <  3:876138/115‑1 (MQ=255)
                                                                   gAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTcc                                                                                <  4:25395/149‑1 (MQ=255)
                                                                    aaGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATg                                                                                                                                   >  4:341866/1‑97 (MQ=255)
                                                                    aaGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATg                                                                                                                                   <  3:341866/97‑1 (MQ=255)
                                                                     aGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTccc                                                                               <  1:541088/148‑1 (MQ=255)
                                                                      gCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCg                                                                              <  2:571767/148‑1 (MQ=255)
                                                                       cccAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCgg                                                                             >  1:70040/1‑148 (MQ=255)
                                                                         cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgt                                                                          <  4:266055/149‑1 (MQ=255)
                                                                         cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtg                                                                           <  2:753795/148‑1 (MQ=255)
                                                                           gCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCAGCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgtg                                                                         <  2:417600/148‑1 (MQ=255)
                                                                           gCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgtg                                                                         >  1:417600/1‑148 (MQ=255)
                                                                                aGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAAAGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTAtt                                                                   >  3:596201/1‑149 (MQ=255)
                                                                                      cACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgtg                                                             >  3:257778/1‑149 (MQ=255)
                                                                                       aCCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  4:501126/1‑149 (MQ=255)
                                                                                        ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATCGTGTTGATTGCACCAGAGACTGGCGATAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  1:701043/1‑148 (MQ=255)
                                                                                        ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  2:774451/1‑148 (MQ=255)
                                                                                        ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  1:494265/1‑148 (MQ=255)
                                                                                               gcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              >  4:426514/1‑139 (MQ=255)
                                                                                               gcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              <  3:426514/139‑1 (MQ=255)
                                                                                                 gATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGc                                                  <  4:735475/149‑1 (MQ=255)
                                                                                                 gATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTATATTTTTGTGCCACAATACg                                                   <  1:505683/148‑1 (MQ=255)
                                                                                                   tAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCt                                                 <  2:686885/148‑1 (MQ=255)
                                                                                                       cGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGt                                            >  4:469067/1‑149 (MQ=255)
                                                                                                               aGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCg                                     <  1:596144/148‑1 (MQ=255)
                                                                                                                 cGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGtt                                   >  2:270177/1‑148 (MQ=255)
                                                                                                                   aGGTGGTCATGGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  3:821204/67‑1 (MQ=255)
                                                                                                                   aGGTGGTCATGGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  >  4:821204/1‑67 (MQ=255)
                                                                                                                     gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc                              <  3:146258/149‑1 (MQ=255)
                                                                                                                     gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc                              <  3:355795/149‑1 (MQ=255)
                                                                                                                      tgggCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAACGAGGTCGTCAGGTAGGCAAACCACCATGCTAAGGTCATGATGAACTCCCTGTTTGTTTATTTTTGTGCCACAATACTCTCCTGTCGCAACGTTGTGTc                              <  2:886856/148‑1 (MQ=255)
                                                                                                                        gtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAgg                           <  4:892881/149‑1 (MQ=255)
                                                                                                                        gtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAgg                           >  3:143589/1‑149 (MQ=255)
                                                                                                                          cATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGc                          >  2:851806/1‑148 (MQ=255)
                                                                                                                           aTAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                >  4:170483/1‑45 (MQ=255)
                                                                                                                           aTAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                <  3:170483/45‑1 (MQ=255)
                                                                                                                             aGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACg                       >  2:178539/1‑148 (MQ=255)
                                                                                                                              gtgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAg                            <  4:496834/142‑1 (MQ=255)
                                                                                                                              gtgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAg                            >  3:496834/1‑142 (MQ=255)
                                                                                                                               tgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCt                     >  1:867712/1‑148 (MQ=255)
                                                                                                                                 ttGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaa                   >  1:77464/1‑148 (MQ=255)
                                                                                                                                  tGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaa                  >  2:347278/1‑148 (MQ=255)
                                                                                                                                  tGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATCTCATCAGGGAGGGAAACCACCATTCTAAGGACATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaa                  >  2:843304/1‑148 (MQ=255)
                                                                                                                                   gATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              >  4:325008/1‑103 (MQ=255)
                                                                                                                                   gATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              <  3:325008/103‑1 (MQ=255)
                                                                                                                                      tGCACCAGAGCCTGGCGACAGGCTTAAAATGATGGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaaaaaaa             <  4:596201/149‑1 (MQ=255)
                                                                                                                                      tGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaaaaaa              >  1:708189/1‑148 (MQ=255)
                                                                                                                                       cattttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAAc            >  3:245586/8‑149 (MQ=255)
                                                                                                                                        cACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAAc            >  1:904481/1‑148 (MQ=255)
                                                                                                                                         aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCca                                                          >  4:369944/1‑101 (MQ=255)
                                                                                                                                         aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCca                                                          <  3:369944/101‑1 (MQ=255)
                                                                                                                                         aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGa          >  3:424259/1‑149 (MQ=255)
                                                                                                                                          tttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCa                             >  2:586476/5‑129 (MQ=255)
                                                                                                                                          tttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCa                             <  1:586476/125‑1 (MQ=255)
                                                                                                                                          ccAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGAt         >  3:484309/1‑149 (MQ=255)
                                                                                                                                              agCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGAtttt      >  2:662399/1‑148 (MQ=255)
                                                                                                                                                 cTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGt  >  3:82363/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGT  >  NC_000913/4008854‑4009147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: