Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,009,005 A→C 5.9% S19A (TCG→GCG)  rhtB ← homoserine, homoserine lactone and S‑methyl‑methionine efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,009,0050AC 5.9% 361.3 / 11.5 102S19A (TCG→GCG) rhtBhomoserine, homoserine lactone and S‑methyl‑methionine efflux pump
Reads supporting (aligned to +/- strand):  ref base A (47/49);  new base C (3/3);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 102 total aligned reads displayed.

GGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGT  >  NC_000913/4008862‑4009147
                                                                                                                                               |                                                                                                                                              
ggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAgg                                                                                                                                            >  2:718645/1‑148 (MQ=255)
ggAAAATAGCGTCACCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCTCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACCGGc                                                                                                                                           <  4:771083/149‑1 (MQ=255)
  aaaaTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtc                                                                                                                                         <  4:26433/149‑2 (MQ=255)
        gCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTTATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAAt                                                                                                                                    <  2:887049/148‑1 (MQ=255)
         cGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGa                                                                                                                                  <  3:625774/149‑1 (MQ=255)
          gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGa                                                                                                                                  <  1:11249/148‑1 (MQ=255)
          gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  3:308546/149‑1 (MQ=255)
          gTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGCTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGTCTTAAAATGa                                                                                                                                  <  1:743035/148‑1 (MQ=255)
           tCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  1:752635/148‑1 (MQ=255)
           tCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                <  4:23131/149‑1 (MQ=255)
            ccccAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                >  1:825036/1‑148 (MQ=255)
              ccAACCCCCCGCCAACCCGCACAATATGAATCGCCAGTCCGGTCTAAAGCACAGCAATAGACGTCACCGCGCCGCGATAACCGTGGTTCAGCGATGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAACTGATCGAt                                                                                                                             <  4:322812/149‑1 (MQ=255)
               cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTaaa                                                                                                                                      >  1:517980/1‑139 (MQ=255)
               cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTaaa                                                                                                                                      <  2:517980/139‑1 (MQ=255)
               cAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGAt                                                                                                                             >  1:463340/1‑148 (MQ=255)
                aaCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGt                                                                                                                           <  3:483480/149‑1 (MQ=255)
                    ccACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTcag                                                                                                                        <  2:259315/148‑1 (MQ=255)
                      aCGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTcagca                                                                                                                      >  2:596144/1‑148 (MQ=255)
                         ccAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   >  2:622815/1‑148 (MQ=255)
                         ccAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   <  1:768548/148‑1 (MQ=255)
                          cAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  2:478308/148‑1 (MQ=255)
                           aaCCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAgg                                                                                                                >  3:543682/1‑149 (MQ=255)
                                gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 <  3:818553/127‑1 (MQ=255)
                                gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAt                                                                                                                                 >  4:818553/1‑127 (MQ=255)
                                gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAac                                                                                                           <  4:338570/149‑1 (MQ=255)
                                  acaATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACa                                                                                                                                              >  1:893615/1‑112 (MQ=255)
                                  acaATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACa                                                                                                                                              <  2:893615/112‑1 (MQ=255)
                                   caATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAaccac                                                                                                        >  3:591094/1‑149 (MQ=255)
                                    aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtctg                                                                                                                                       <  4:798828/117‑4 (MQ=255)
                                    aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGTCGCCAGGCtctg                                                                                                                                       >  3:798828/1‑114 (MQ=255)
                                    aaTATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAaccacc                                                                                                       >  4:31560/1‑149 (MQ=255)
                                       atGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCAt                                                                                                     <  1:115213/148‑1 (MQ=255)
                                       atGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCAt                                                                                                     >  1:445205/1‑148 (MQ=255)
                                           aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTaa                                                                                                <  3:31560/149‑1 (MQ=255)
                                           aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTa                                                                                                 <  2:677341/148‑1 (MQ=255)
                                            tCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTaa                                                                                                >  2:352876/1‑148 (MQ=255)
                                            tCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAg                                                                                               <  4:591094/149‑1 (MQ=255)
                                             cGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  >  2:410477/1‑129 (MQ=255)
                                             cGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  1:410477/129‑1 (MQ=255)
                                               ccAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTc                                                                                            >  3:266055/1‑149 (MQ=255)
                                               ccAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTc                                                                                            >  4:343980/1‑149 (MQ=255)
                                                   tCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCatg                                                                                         <  1:352876/148‑1 (MQ=255)
                                                        tCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGaa                                                                                    <  1:718645/148‑1 (MQ=255)
                                                          tGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   <  3:876138/115‑1 (MQ=255)
                                                          tGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGt                                                                                                                   >  4:876138/1‑115 (MQ=255)
                                                           gAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTcc                                                                                <  4:25395/149‑1 (MQ=255)
                                                            aaGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATg                                                                                                                                   >  4:341866/1‑97 (MQ=255)
                                                            aaGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATg                                                                                                                                   <  3:341866/97‑1 (MQ=255)
                                                             aGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTccc                                                                               <  1:541088/148‑1 (MQ=255)
                                                              gCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCg                                                                              <  2:571767/148‑1 (MQ=255)
                                                               cccAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCgg                                                                             >  1:70040/1‑148 (MQ=255)
                                                                 cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgt                                                                          <  4:266055/149‑1 (MQ=255)
                                                                 cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtg                                                                           <  2:753795/148‑1 (MQ=255)
                                                                   gCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCAGCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgtg                                                                         <  2:417600/148‑1 (MQ=255)
                                                                   gCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGgtgtg                                                                         >  1:417600/1‑148 (MQ=255)
                                                                        aGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAAAGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTAtt                                                                   >  3:596201/1‑149 (MQ=255)
                                                                           cGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTAtttt                                                                 <  2:567625/148‑1 (MQ=255)
                                                                              cACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgtg                                                             >  3:257778/1‑149 (MQ=255)
                                                                               aCCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  4:501126/1‑149 (MQ=255)
                                                                                ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATCGTGTTGATTGCACCAGAGACTGGCGATAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  1:701043/1‑148 (MQ=255)
                                                                                ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  2:774451/1‑148 (MQ=255)
                                                                                ccgcgccgcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGc                                                            >  1:494265/1‑148 (MQ=255)
                                                                                       gcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              >  4:426514/1‑139 (MQ=255)
                                                                                       gcgATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              <  3:426514/139‑1 (MQ=255)
                                                                                         gATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGc                                                  <  4:735475/149‑1 (MQ=255)
                                                                                         gATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTATATTTTTGTGCCACAATACg                                                   <  1:505683/148‑1 (MQ=255)
                                                                                           tAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCt                                                 <  2:686885/148‑1 (MQ=255)
                                                                                               cGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGt                                            >  4:469067/1‑149 (MQ=255)
                                                                                                       aGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCg                                     <  1:596144/148‑1 (MQ=255)
                                                                                                         cGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGtt                                   >  2:270177/1‑148 (MQ=255)
                                                                                                           aGGTGGTCATGGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  >  4:821204/1‑67 (MQ=255)
                                                                                                           aGGTGGTCATGGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTa                                                                                                                  <  3:821204/67‑1 (MQ=255)
                                                                                                             gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc                              <  3:146258/149‑1 (MQ=255)
                                                                                                             gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc                              <  3:355795/149‑1 (MQ=255)
                                                                                                              tgggCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGAACGAGGTCGTCAGGTAGGCAAACCACCATGCTAAGGTCATGATGAACTCCCTGTTTGTTTATTTTTGTGCCACAATACTCTCCTGTCGCAACGTTGTGTc                              <  2:886856/148‑1 (MQ=255)
                                                                                                                gtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAgg                           <  4:892881/149‑1 (MQ=255)
                                                                                                                  cATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGc                          >  2:851806/1‑148 (MQ=255)
                                                                                                                   aTAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                >  4:170483/1‑45 (MQ=255)
                                                                                                                   aTAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATc                                                                                                                                <  3:170483/45‑1 (MQ=255)
                                                                                                                     aGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACg                       >  2:178539/1‑148 (MQ=255)
                                                                                                                      gtgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAg                            >  3:496834/1‑142 (MQ=255)
                                                                                                                      gtgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAg                            <  4:496834/142‑1 (MQ=255)
                                                                                                                       tgtTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCt                     >  1:867712/1‑148 (MQ=255)
                                                                                                                         ttGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaa                   >  1:77464/1‑148 (MQ=255)
                                                                                                                          tGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaa                  >  2:347278/1‑148 (MQ=255)
                                                                                                                          tGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATCTCATCAGGGAGGGAAACCACCATTCTAAGGACATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaa                  >  2:843304/1‑148 (MQ=255)
                                                                                                                           gATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              >  4:325008/1‑103 (MQ=255)
                                                                                                                           gATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTtgt                                                              <  3:325008/103‑1 (MQ=255)
                                                                                                                              tGCACCAGAGCCTGGCGACAGGCTTAAAATGATGGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaaaaaaa             <  4:596201/149‑1 (MQ=255)
                                                                                                                              tGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTaaaaaaa              >  1:708189/1‑148 (MQ=255)
                                                                                                                               cattttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAAc            >  3:245586/8‑149 (MQ=255)
                                                                                                                                cACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAAc            >  1:904481/1‑148 (MQ=255)
                                                                                                                                 aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCca                                                          >  4:369944/1‑101 (MQ=255)
                                                                                                                                 aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCca                                                          <  3:369944/101‑1 (MQ=255)
                                                                                                                                 aCCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGa          >  3:424259/1‑149 (MQ=255)
                                                                                                                                  tttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCa                             >  2:586476/5‑129 (MQ=255)
                                                                                                                                  tttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCa                             <  1:586476/125‑1 (MQ=255)
                                                                                                                                  ccAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGAt         >  3:484309/1‑149 (MQ=255)
                                                                                                                                      agCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGAtttt      >  2:662399/1‑148 (MQ=255)
                                                                                                                                         cTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGt  >  3:82363/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGT  >  NC_000913/4008862‑4009147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: