Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,621 T→G 6.2% Q203P (CAA→CCA)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,6210TG 6.2% 395.9 / 15.9 113Q203P (CAA→CCA) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base T (54/52);  new base G (3/4);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 115 total aligned reads displayed.

GGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATCG  >  NC_000913/4080473‑4080767
                                                                                                                                                    |                                                                                                                                                  
ggCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAAttt                                                                                                                                                    >  3:419458/1‑149 (MQ=255)
  cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTg                                                                                                                                                   >  1:68650/1‑148 (MQ=255)
  cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGc                                                                                                                                                  >  4:538126/1‑149 (MQ=255)
      aaTCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCa                                                                                                                                              <  4:830736/149‑1 (MQ=255)
          gcgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactca                                                                                                                                          <  4:495198/149‑5 (MQ=255)
           cgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCt                                                                                                                                          <  2:613515/148‑1 (MQ=255)
                       cATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCAt                                                                                                                              <  1:622514/148‑1 (MQ=255)
                         tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAg                                                                                                                            >  1:862246/1‑148 (MQ=255)
                         tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                           >  4:847286/1‑149 (MQ=255)
                          ccAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                           <  2:700639/148‑1 (MQ=255)
                            aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAcc                                                                                                                        >  3:705604/1‑149 (MQ=255)
                             gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                           >  1:447105/1‑145 (MQ=255)
                             gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                           <  2:447105/145‑1 (MQ=255)
                             gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGACACCGGCATAGAACcac                                                                                                                      <  3:111749/149‑1 (MQ=255)
                                 ccAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACaaa                                                                                                                   <  3:847286/149‑1 (MQ=255)
                                  cAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAc                                                                                                                  >  3:852695/1‑149 (MQ=255)
                                     tAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg                                                                                                               <  4:374892/149‑1 (MQ=255)
                                        tGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCa                                                                                                            >  3:399948/1‑149 (MQ=255)
                                        tGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCa                                                                                                            >  4:822838/1‑149 (MQ=255)
                                         gCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCaa                                                                                                           >  3:61923/1‑149 (MQ=255)
                                          cAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAat                                                                                                          >  3:841319/1‑149 (MQ=255)
                                               tGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGa                                                                                                     <  4:794090/149‑1 (MQ=255)
                                                gCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAc                                                                                                    <  4:841319/149‑1 (MQ=255)
                                                gCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAc                                                                                                    <  3:557153/149‑1 (MQ=255)
                                                   gCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCAc                                                                                                                                                <  4:537211/102‑1 (MQ=255)
                                                   gCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCAc                                                                                                                                                >  3:537211/1‑102 (MQ=255)
                                                     tctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGc                                                                                               <  4:705604/149‑1 (MQ=255)
                                                          tCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGTAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGcc                                                                                          >  4:101693/1‑149 (MQ=255)
                                                          tCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGcc                                                                                          >  4:626715/1‑149 (MQ=255)
                                                            gCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGt                                                                                        <  3:702766/149‑1 (MQ=255)
                                                             cAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGt                                                                                        >  2:615862/1‑148 (MQ=255)
                                                             cAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTa                                                                                       <  4:650046/149‑1 (MQ=255)
                                                                ttGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTc                                                                                    >  4:247814/1‑149 (MQ=255)
                                                                 tgctgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTc                                                                                    <  1:488352/148‑1 (MQ=255)
                                                                  gctgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTAt                                                                                      <  4:720742/145‑1 (MQ=255)
                                                                  gctgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTAt                                                                                      >  3:720742/1‑145 (MQ=255)
                                                                    tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACaaa                                                                                                                   >  4:811901/1‑114 (MQ=255)
                                                                    tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                                >  3:244968/1‑149 (MQ=255)
                                                                     gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                                <  1:175582/148‑1 (MQ=255)
                                                                     gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                                >  2:412421/1‑148 (MQ=255)
                                                                     gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                                >  1:301490/1‑148 (MQ=255)
                                                                     gcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                                >  2:499562/1‑148 (MQ=255)
                                                                      cATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa                                                                                                                                                 >  3:55518/1‑82 (MQ=255)
                                                                      cATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa                                                                                                                                                 <  4:55518/82‑1 (MQ=255)
                                                                                 cACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgg                                                                   <  3:573618/149‑1 (MQ=255)
                                                                                    tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgggg                                                                 <  1:499562/148‑1 (MQ=255)
                                                                                    tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                                <  3:231736/149‑1 (MQ=255)
                                                                                    tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATACTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg                                                                  >  4:395511/1‑147 (MQ=255)
                                                                                    tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATACTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg                                                                  <  3:395511/147‑1 (MQ=255)
                                                                                     aCGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                                <  2:32701/148‑1 (MQ=255)
                                                                                         gCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa                                                           <  4:266407/149‑1 (MQ=255)
                                                                                           cATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAt                                                          <  2:68650/148‑1 (MQ=255)
                                                                                            aTTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                        <  3:456936/149‑1 (MQ=255)
                                                                                             ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                        <  1:844732/148‑1 (MQ=255)
                                                                                              tCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGc                                                      >  3:656755/1‑149 (MQ=255)
                                                                                                  tttCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATc                                                  >  4:762402/1‑149 (MQ=255)
                                                                                                   ttCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATc                                                  >  1:309843/1‑148 (MQ=255)
                                                                                                   ttCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCt                                                 <  3:775705/149‑1 (MQ=255)
                                                                                                   ttCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCt                                                 <  3:822838/149‑1 (MQ=255)
                                                                                                    tCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAg                                                            <  2:350534/137‑1 (MQ=255)
                                                                                                    tCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAg                                                            >  1:350534/1‑137 (MQ=255)
                                                                                                     cacacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATc                                                              >  1:165934/1‑134 (MQ=255)
                                                                                                     cacacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATc                                                              <  2:165934/134‑1 (MQ=255)
                                                                                                      acacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                              >  4:258803/1‑149 (MQ=255)
                                                                                                       cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcgc                                             <  3:846032/149‑1 (MQ=255)
                                                                                                       cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                              >  1:398367/1‑148 (MQ=255)
                                                                                                       cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                              >  1:402721/1‑148 (MQ=255)
                                                                                                       cacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                              >  1:623827/1‑148 (MQ=255)
                                                                                                        acaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                          <  2:413499/71‑1 (MQ=255)
                                                                                                        acaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                          >  1:413499/1‑71 (MQ=255)
                                                                                                        acaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGcc                                            >  4:829558/1‑149 (MQ=255)
                                                                                                         caGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGcc                                            <  1:615862/148‑1 (MQ=255)
                                                                                                          aGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCa                                          <  3:762402/149‑1 (MQ=255)
                                                                                                           ggTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCa                                          >  1:217705/1‑148 (MQ=255)
                                                                                                             ttGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGt                                        >  2:784770/1‑148 (MQ=255)
                                                                                                                  gtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCg                                  >  3:387480/1‑149 (MQ=255)
                                                                                                                   tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa                                 <  4:244968/149‑1 (MQ=255)
                                                                                                                      agGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAg                              >  3:27612/1‑149 (MQ=255)
                                                                                                                      agGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAg                              >  4:768993/1‑149 (MQ=255)
                                                                                                                       ggTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGt                             >  3:328244/1‑149 (MQ=255)
                                                                                                                          aaCGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCa                          >  3:810891/1‑149 (MQ=255)
                                                                                                                             gTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGc                        <  2:623827/148‑1 (MQ=255)
                                                                                                                              tAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGcc                       >  1:659720/1‑148 (MQ=255)
                                                                                                                              tAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGcc                       >  1:649833/1‑148 (MQ=255)
                                                                                                                              tAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGcc                       <  2:659720/148‑1 (MQ=255)
                                                                                                                                 ccccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGa                   >  4:204393/1‑149 (MQ=255)
                                                                                                                                   ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                                <  4:165604/102‑1 (MQ=255)
                                                                                                                                   ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa                                                                >  3:165604/1‑102 (MQ=255)
                                                                                                                                   ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGccc                      <  1:82986/144‑1 (MQ=255)
                                                                                                                                   ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGccc                      >  2:82986/1‑144 (MQ=255)
                                                                                                                                    cTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATa                 >  2:19726/1‑148 (MQ=255)
                                                                                                                                     tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa                                 >  2:832710/1‑131 (MQ=255)
                                                                                                                                     tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa                                 <  1:832710/131‑1 (MQ=255)
                                                                                                                                      gcatggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATaaa               >  1:238272/4‑148 (MQ=255)
                                                                                                                                       catggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                        <  1:312329/104‑1 (MQ=255)
                                                                                                                                       catggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAAc              >  2:472577/3‑148 (MQ=255)
                                                                                                                                         tggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACgg            <  2:217705/148‑1 (MQ=255)
                                                                                                                                           gtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGgcgc         <  3:538126/149‑1 (MQ=255)
                                                                                                                                               cAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTa     >  3:82737/1‑149 (MQ=255)
                                                                                                                                                 aTTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATc   >  4:772953/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATCG  >  NC_000913/4080473‑4080767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: