| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,193,185 | G→C | 7.1% | T131S (ACT→AGT) | thiF ← | adenylyltransferase, modifies ThiS C‑terminus |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,193,185 | 0 | G | C | 7.1% | 393.2 / 17.2 | 112 | T131S (ACT→AGT) | thiF | adenylyltransferase, modifies ThiS C‑terminus |
| Reads supporting (aligned to +/- strand): ref base G (52/52); new base C (5/3); total (57/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GGCTCCTGGTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCG > NC_000913/4193038‑4193332 | ggCTCCTGGTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAg > 2:473382/1‑148 (MQ=255) gCTCCTGGTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCATCTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTCAGTGCCACGCAGGCGGCATTAATGTCCTGGCGAGTc < 4:566370/149‑1 (MQ=255) cTCCTGGTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCg > 3:150084/1‑149 (MQ=255) gTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCata < 2:137683/148‑1 (MQ=255) gTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCGCCagga < 4:150084/149‑4 (MQ=255) ttATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCGCCagg > 1:9127/1‑145 (MQ=255) ttATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCGCCagg < 2:9127/147‑3 (MQ=255) aTCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGt < 3:502214/149‑1 (MQ=255) cTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGCGTCGCCATATTGTCg < 4:668358/149‑1 (MQ=255) cTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTc < 1:528504/148‑1 (MQ=255) tGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCgg > 3:457598/1‑149 (MQ=255) acaGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAg > 3:324846/1‑149 (MQ=255) gCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTc > 4:686363/1‑147 (MQ=255) gCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTc < 3:686363/147‑1 (MQ=255) cAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGa > 1:240307/1‑148 (MQ=255) cAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCGCCaggag < 2:311524/134‑5 (MQ=255) cAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGACTCGCCaggag > 1:311524/1‑130 (MQ=255) cGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGcac < 4:444042/149‑1 (MQ=255) acCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCg > 3:33805/1‑149 (MQ=255) ccccTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCatat > 2:310362/1‑122 (MQ=255) ccccTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCatat < 1:310362/122‑1 (MQ=255) cccTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTTATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGc > 3:274650/1‑149 (MQ=255) cccTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTAGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGc > 3:756976/1‑149 (MQ=255) cTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGcc > 2:42976/1‑148 (MQ=255) cTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTAAAGTCGAGCACCACATCGGcc < 2:201646/148‑1 (MQ=255) tGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGccc > 2:838641/1‑148 (MQ=255) gCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGCACAGTCGAGCACCACATCGGCCCg < 2:136590/148‑1 (MQ=255) gCTCCCAGGGCAGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGt < 4:756976/149‑1 (MQ=255) ccAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCaa > 4:142887/1‑149 (MQ=255) ccAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCaa > 4:409443/1‑149 (MQ=255) cAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCaa < 2:638709/148‑1 (MQ=255) aGGACGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAAcc > 3:483384/1‑149 (MQ=255) cggcgTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCa < 1:42976/148‑1 (MQ=255) ggcgTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATc < 3:346174/149‑1 (MQ=255) ggcgTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATc < 4:580747/149‑1 (MQ=255) gcgTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCt < 4:314940/149‑1 (MQ=255) gTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCa > 1:48713/1‑100 (MQ=255) gTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCa < 2:48713/100‑1 (MQ=255) gTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTaa > 1:219303/1‑148 (MQ=255) aCCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACg > 1:179664/1‑148 (MQ=255) cATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCt > 4:244220/1‑149 (MQ=255) cAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCa > 1:129548/1‑148 (MQ=255) tGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGt > 3:158/1‑149 (MQ=255) gACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGt < 2:394508/148‑1 (MQ=255) cGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAAc > 3:595571/1‑149 (MQ=255) ccAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTc > 4:204583/1‑75 (MQ=255) ccAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTc < 3:204583/75‑1 (MQ=255) ccAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCg > 4:799393/1‑149 (MQ=255) cAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACa > 2:713134/1‑91 (MQ=255) cAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACa < 1:713134/91‑1 (MQ=255) cAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCg > 2:18654/1‑148 (MQ=255) cAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCg > 1:459043/1‑148 (MQ=255) aaaTCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGcac < 1:508538/99‑1 (MQ=255) aaaTCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGcac > 2:508538/1‑99 (MQ=255) tCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCgttgtt < 4:715902/149‑1 (MQ=255) gACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTtgttgt > 1:479998/1‑148 (MQ=255) gACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTa > 4:789803/1‑149 (MQ=255) aCCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTa > 1:687244/1‑148 (MQ=255) aCCGCGCTGGCGGTGATAAGCGGCGTGATGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTa > 1:96717/1‑148 (MQ=255) tGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGt < 1:26570/148‑1 (MQ=255) ggTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGtt < 4:595571/149‑1 (MQ=255) aTAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGaa > 2:13998/1‑148 (MQ=255) gcggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCg < 4:158/149‑1 (MQ=255) gcggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCg < 3:799393/149‑1 (MQ=255) cggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCgg < 3:568491/149‑1 (MQ=255) ggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTg > 2:67693/1‑140 (MQ=255) ggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTg < 1:67693/140‑1 (MQ=255) gcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGa < 3:535475/149‑1 (MQ=255) gcgTGTTGAGTCCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCggg < 1:741171/148‑1 (MQ=255) tgtTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGAtt < 1:473382/148‑1 (MQ=255) ttGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCaa < 3:789803/149‑1 (MQ=255) tGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTc < 3:186122/139‑1 (MQ=255) tGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTc > 4:186122/1‑139 (MQ=255) tGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACtgtg > 4:315966/1‑149 (MQ=255) cACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCa < 4:324846/149‑1 (MQ=255) cACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCa < 3:142887/149‑1 (MQ=255) cggcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTg < 2:28933/148‑1 (MQ=255) ggcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGtt < 4:33805/149‑1 (MQ=255) gcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGAtt > 4:599030/1‑128 (MQ=255) gcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGAtt < 3:599030/128‑1 (MQ=255) gcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTg < 4:457598/149‑1 (MQ=255) ttAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCt < 3:64679/149‑1 (MQ=255) aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCgtt < 3:457437/91‑1 (MQ=255) aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCgtt > 4:457437/1‑91 (MQ=255) aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAc > 3:31935/1‑149 (MQ=255) tctcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAcc > 3:374610/1‑149 (MQ=255) tctcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAc > 1:789000/1‑148 (MQ=255) ctcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCt < 3:451089/149‑1 (MQ=255) tcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACt < 4:281436/124‑1 (MQ=255) tcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACt > 3:281436/1‑124 (MQ=255) tcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTg > 4:548366/1‑149 (MQ=255) tatGGCGACTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGc > 3:904635/3‑149 (MQ=255) ccTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCa < 2:301897/74‑1 (MQ=255) ccTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCa > 1:301897/1‑74 (MQ=255) ccTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTg < 2:129548/148‑1 (MQ=255) atGGCGACTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGc > 1:806931/2‑148 (MQ=255) tGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGa > 4:84553/1‑149 (MQ=255) gCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGAtt > 3:38712/1‑149 (MQ=255) aGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTc < 1:42547/148‑1 (MQ=255) gTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCg < 2:240307/148‑1 (MQ=255) | GGCTCCTGGTTATCTGGCCACAGGCAGCGGTAACACCCCTGCTCCCAGGGCGGCGTCAGTACCATCAACTGACCGCCAAATCCGACCGCGCTGGCGGTGATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCG > NC_000913/4193038‑4193332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |