Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,245,113 C→A 5.2% intergenic (‑38/+116) malF ← / ← malE maltose transporter subunit/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,245,1130CA 5.2% 415.2 / 8.9 116intergenic (‑38/+116)malF/malEmaltose transporter subunit/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (57/53);  new base A (3/3);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 118 total aligned reads displayed.

TTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCA  >  NC_000913/4244965‑4245260
                                                                                                                                                    |                                                                                                                                                   
ttGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCAc                                                                                                                                                     <  4:453065/149‑1 (MQ=255)
   tgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGcc                                                                                                                                                  <  4:887807/149‑1 (MQ=255)
      gTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTa                                                                                                                                               <  3:470544/149‑1 (MQ=255)
       tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                              >  4:675385/1‑149 (MQ=255)
        aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                             >  3:889623/1‑149 (MQ=255)
           ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                           >  2:876666/1‑148 (MQ=255)
           ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                           <  2:611260/148‑1 (MQ=255)
           ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                           >  1:86719/1‑148 (MQ=255)
           ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          >  4:399755/1‑149 (MQ=255)
            tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGc                                                                                                                                                   >  1:127868/1‑139 (MQ=255)
            tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGc                                                                                                                                                   <  2:127868/139‑1 (MQ=255)
            tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          >  1:67814/1‑148 (MQ=255)
              aaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaa                                                                                                                                       >  4:169992/1‑149 (MQ=255)
              aaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGaca                                                                                                                                        <  2:151823/148‑1 (MQ=255)
               aacaacAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaa                                                                                                                                       >  1:504238/1‑148 (MQ=255)
               aacaacAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaa                                                                                                                                       >  2:877173/1‑148 (MQ=255)
                acaacaAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaac                                                                                                                                      <  2:50983/148‑1 (MQ=255)
                  aacaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                                   >  4:610977/1‑149 (MQ=255)
                   acaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACt                                                                                                                                  >  3:141125/1‑149 (MQ=255)
                      aGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGc                                                                                                                                <  1:876666/148‑1 (MQ=255)
                        gTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCt                                                                                                                              >  1:614191/1‑148 (MQ=255)
                        gTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTg                                                                                                                             >  4:509726/1‑149 (MQ=255)
                          aaCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGAt                                                                                                                           <  3:599636/149‑1 (MQ=255)
                           aCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCTACAACAACTg                                                                                                                                 <  1:676922/142‑1 (MQ=255)
                           aCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTg                                                                                                                                 >  2:676922/1‑142 (MQ=255)
                            cccACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATg                                                                                                                          >  2:151324/1‑148 (MQ=255)
                              caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATc                                                                                                                                             <  3:447840/127‑1 (MQ=255)
                              caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATc                                                                                                                                             >  4:447840/1‑127 (MQ=255)
                               accaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGTACGGATAAGGCTTTCACGCCTTAt                                                                                                                                              >  2:749241/1‑125 (MQ=255)
                               accaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                              <  1:749241/125‑1 (MQ=255)
                               accaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGCTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGAc                                                                                                                      >  3:888827/1‑149 (MQ=255)
                                 cagcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGc                                                                                                                    <  4:185679/149‑1 (MQ=255)
                                   gcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATcc                                                                                                                                            <  3:823287/123‑1 (MQ=255)
                                   gcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATcc                                                                                                                                            >  4:823287/1‑123 (MQ=255)
                                    cagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTg                                                                                                                  <  2:67814/148‑1 (MQ=255)
                                      ggCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAc                                                                                                                >  2:616854/1‑148 (MQ=255)
                                       gCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcg                                                                                                               <  1:151324/148‑1 (MQ=255)
                                          gAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                           <  4:63441/149‑1 (MQ=255)
                                          gAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                           <  4:141125/149‑1 (MQ=255)
                                           aGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                           >  1:257509/1‑148 (MQ=255)
                                              aGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTa                                                                                                        <  1:780814/148‑1 (MQ=255)
                                              aGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATGCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTAt                                                                                                       >  4:884252/1‑149 (MQ=255)
                                                 ccTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                              >  1:355970/1‑107 (MQ=255)
                                                     gCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                                 <  1:877173/148‑1 (MQ=255)
                                                       aCTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTa                                                                                               <  2:914709/148‑1 (MQ=255)
                                                               tttCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGttt                                                                                      <  3:318439/149‑1 (MQ=255)
                                                                   aGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGt                                                                                  <  3:610977/149‑1 (MQ=255)
                                                                    gcgcGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                                 <  4:66341/149‑1 (MQ=255)
                                                                     cgcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                                 <  2:257509/148‑1 (MQ=255)
                                                                      gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                               <  3:639784/149‑1 (MQ=255)
                                                                         tCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                                 <  3:460574/144‑1 (MQ=255)
                                                                         tCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                                 >  4:460574/1‑144 (MQ=255)
                                                                             tttGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGcc                                                                        <  3:675385/149‑1 (MQ=255)
                                                                                  caccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                         <  4:357815/79‑1 (MQ=255)
                                                                                  caccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                         >  3:357815/1‑79 (MQ=255)
                                                                                    ccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                                  >  1:184816/1‑148 (MQ=255)
                                                                                     caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                          >  1:256949/1‑107 (MQ=255)
                                                                                     caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                          <  2:256949/107‑1 (MQ=255)
                                                                                       aTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCg                                                              <  4:484874/149‑1 (MQ=255)
                                                                                        tGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCg                                                              >  1:350836/1‑148 (MQ=255)
                                                                                           ttCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGttt                                                                                      >  1:642371/1‑121 (MQ=255)
                                                                                           ttCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGttt                                                                                      <  2:642371/121‑1 (MQ=255)
                                                                                           ttCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                                  >  2:46649/1‑141 (MQ=255)
                                                                                           ttCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                                  <  1:46649/141‑1 (MQ=255)
                                                                                              tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  3:699332/1‑149 (MQ=255)
                                                                                              tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                       >  4:377878/1‑149 (MQ=255)
                                                                                               ttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgcc                                                      >  3:641965/1‑149 (MQ=255)
                                                                                                     tGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATc                                                 <  2:350836/148‑1 (MQ=255)
                                                                                                      gACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACACTGACGCGTc                                                                                                           >  3:686967/1‑89 (MQ=255)
                                                                                                         aTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          <  3:318596/55‑1 (MQ=255)
                                                                                                         aTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                          >  4:318596/1‑55 (MQ=255)
                                                                                                         aTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTGTCCGGcc                                            <  3:875110/149‑2 (MQ=37)
                                                                                                          tCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCa                                            >  1:815647/1‑148 (MQ=255)
                                                                                                              tGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTc                                        <  1:355852/148‑1 (MQ=255)
                                                                                                                  gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGc                                   >  4:332079/1‑149 (MQ=255)
                                                                                                                      ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTATGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTa                               >  4:593058/1‑149 (MQ=255)
                                                                                                                      ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTa                               >  4:219048/1‑149 (MQ=255)
                                                                                                                         cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACtt                            >  4:630934/1‑149 (MQ=255)
                                                                                                                          tCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTg                           >  4:102624/1‑149 (MQ=255)
                                                                                                                           cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                          <  4:699332/149‑1 (MQ=255)
                                                                                                                           cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTg                           >  2:404621/1‑148 (MQ=255)
                                                                                                                             ttCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgcc                                                      <  3:881567/119‑1 (MQ=255)
                                                                                                                             ttCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgcc                                                      >  4:881567/1‑119 (MQ=255)
                                                                                                                              tCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                        >  2:604534/1‑148 (MQ=255)
                                                                                                                                 aGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATa                     <  1:616654/148‑1 (MQ=255)
                                                                                                                                   gACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGa                  <  3:102624/149‑1 (MQ=255)
                                                                                                                                     cGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAg                 <  1:604534/148‑1 (MQ=255)
                                                                                                                                      ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                          >  4:282785/1‑122 (MQ=25)
                                                                                                                                      ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                          <  3:282785/122‑1 (MQ=255)
                                                                                                                                        aTAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCt              >  1:761140/1‑148 (MQ=255)
                                                                                                                                        aTAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTg             >  3:39703/1‑149 (MQ=255)
                                                                                                                                         tAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTg             <  2:869564/148‑1 (MQ=255)
                                                                                                                                          aaGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATa                     <  1:119304/139‑1 (MQ=255)
                                                                                                                                           aGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTgcgc          <  4:39703/149‑1 (MQ=255)
                                                                                                                                            ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGcgcg         >  3:453554/1‑149 (MQ=255)
                                                                                                                                              cTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGt        >  1:522885/1‑148 (MQ=255)
                                                                                                                                               tttCACGCCTTATGCGACAACAACTGCCTGATGCGACGCTGACTCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc       <  1:459579/148‑1 (MQ=255)
                                                                                                                                               tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc       <  2:815647/148‑1 (MQ=255)
                                                                                                                                                ttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCt      >  1:912831/1‑148 (MQ=255)
                                                                                                                                                    cGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCa  <  1:404621/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCA  >  NC_000913/4244965‑4245260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: