Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,270,142 G→A 9.6% intergenic (+15/‑96) aphA → / → yjbQ acid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,1420GA 9.6% 279.8 / 16.6 94intergenic (+15/‑96)aphA/yjbQacid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (46/39);  new base A (5/4);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

TAATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCAT  >  NC_000913/4269995‑4270288
                                                                                                                                                   |                                                                                                                                                  
tAATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTTAGAATACTGACAGAGCGGGAGAGCGt                                                                                                                                                   <  3:560356/149‑1 (MQ=255)
    gATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGAt                                                                                                                                                <  2:137408/148‑1 (MQ=255)
     atatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATg                                                                                                                                               >  2:658654/1‑148 (MQ=255)
      tatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATATTGACAGAGCGGGAGAGCGTGATGct                                                                                                                                             >  4:309828/1‑149 (MQ=255)
      tatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGc                                                                                                                                              >  2:121906/1‑148 (MQ=255)
        ttACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGctct                                                                                                                                           <  3:333178/149‑1 (MQ=255)
         tACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTATAAACCCTTGCCACAAGCGGTTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCtctc                                                                                                                                          <  3:556275/149‑1 (MQ=255)
          aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCtctc                                                                                                                                          <  1:725731/148‑1 (MQ=255)
          aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc                                                                                                                                         <  3:355541/149‑1 (MQ=255)
           ccgccgCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTcc                                                                                                                                         >  1:506423/1‑148 (MQ=255)
           ccgccgCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGAGTGATGCTCTcc                                                                                                                                         <  1:305616/148‑1 (MQ=255)
            cgccgcACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTccc                                                                                                                                        >  1:675065/1‑148 (MQ=255)
              ccgcACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGa                                                                                                                                      <  2:506423/148‑1 (MQ=255)
                      gATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttt                                                                                                                             >  3:510637/1‑149 (MQ=255)
                       aTGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttt                                                                                                                             >  1:830752/1‑148 (MQ=255)
                         gTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttttt                                                                                                                           <  1:658654/148‑1 (MQ=255)
                            ggCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTa                                                                                                                        <  2:119644/148‑1 (MQ=255)
                             gcgcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAAt                                                                                                                      <  4:510637/149‑1 (MQ=255)
                                      gTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCttt                                                                                                             >  4:763300/1‑149 (MQ=255)
                                        aTCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt                                                                                                           >  3:281682/1‑149 (MQ=255)
                                        aTCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTAt                                                                                                           >  3:92299/1‑149 (MQ=255)
                                         tCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                          >  3:715358/1‑149 (MQ=255)
                                         tCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                          >  4:80653/1‑149 (MQ=255)
                                            gCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCt                                                                                                        >  1:54144/1‑148 (MQ=255)
                                             cATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCtt                                                                                                       >  1:29151/1‑148 (MQ=255)
                                              aTTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCttt                                                                                                      <  1:736893/148‑1 (MQ=255)
                                                    cgggcCTCCAAATCTACCTACAAACCCTTGCCACAAGCTGGTGGGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGGGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTc                                                                                               <  4:356032/146‑1 (MQ=255)
                                                       gcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                     >  3:503810/1‑140 (MQ=255)
                                                       gcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                     <  4:503810/140‑1 (MQ=255)
                                                        ccTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtg                                                                                            <  1:121906/148‑1 (MQ=255)
                                                           ccAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgctg                                                                                         >  2:265727/1‑148 (MQ=255)
                                                                   acctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAAt                                                                                                                      <  3:687878/111‑1 (MQ=255)
                                                                   acctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAAt                                                                                                                      >  4:687878/1‑111 (MQ=255)
                                                                   acctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAt                                                                                >  3:791098/1‑149 (MQ=255)
                                                                     ctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                     <  4:264675/126‑1 (MQ=255)
                                                                     ctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                     >  3:264675/1‑126 (MQ=255)
                                                                      tacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTa                                                                              >  1:8463/1‑148 (MQ=255)
                                                                       acAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTaa                                                                             >  2:849718/1‑148 (MQ=255)
                                                                        cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAag                                                                            >  2:813958/1‑148 (MQ=255)
                                                                          aaCCCTTGCCACAAGCGGGTGAGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagag                                                                          >  2:344728/1‑148 (MQ=255)
                                                                             ccTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGtt                                                                      <  3:763300/149‑1 (MQ=255)
                                                                               ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac                                                                                                                 <  4:258119/104‑1 (MQ=255)
                                                                               ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCacac                                                                                                                 >  3:258119/1‑104 (MQ=255)
                                                                               ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                         >  3:51018/1‑144 (MQ=255)
                                                                               ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                         <  4:51018/144‑1 (MQ=255)
                                                                               ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGtttt                                                                    <  3:80653/149‑1 (MQ=255)
                                                                                 gCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                   <  2:540955/148‑1 (MQ=255)
                                                                                    acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTaa                                                                             >  3:538100/1‑135 (MQ=255)
                                                                                    acaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTaa                                                                             <  4:538100/135‑1 (MQ=255)
                                                                                     caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAg                                                              <  3:256157/149‑1 (MQ=255)
                                                                                      aaGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAg                                                              >  1:727426/1‑148 (MQ=255)
                                                                                           ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagca                                                         >  2:554239/1‑148 (MQ=255)
                                                                                             tGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagcagc                                                       >  1:747868/1‑148 (MQ=255)
                                                                                                gTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGcacac                                                   >  3:78289/1‑148 (MQ=255)
                                                                                                 tttGGTGAAGAGGTGATCGTCAATTTAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc                                                                                                                                         <  2:691334/62‑1 (MQ=25)
                                                                                                 tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTaa                                                                                                                       >  1:714127/1‑80 (MQ=255)
                                                                                                 tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTaa                                                                                                                       <  2:714127/80‑1 (MQ=255)
                                                                                                 tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAt                                                   >  2:700685/1‑148 (MQ=255)
                                                                                                 tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTcc                                                                                                                                         >  1:691334/1‑62 (MQ=37)
                                                                                                   tGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtgt                                                 <  1:813958/148‑1 (MQ=255)
                                                                                                   tGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtgt                                                 <  2:429000/148‑1 (MQ=255)
                                                                                                    ggTGAAGAGGTGATCGTCAATTCAGAATATTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                               <  3:309828/149‑1 (MQ=255)
                                                                                                     gTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGTTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                               <  1:700665/148‑1 (MQ=255)
                                                                                                        aaGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATc                                           >  3:692863/1‑149 (MQ=255)
                                                                                                            ggTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCaaaa                                       >  3:300097/1‑149 (MQ=255)
                                                                                                            ggTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCaaaa                                       <  4:78289/149‑1 (MQ=255)
                                                                                                             gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCaaaa                                       <  2:8463/148‑1 (MQ=255)
                                                                                                             gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAg                                      >  4:383597/1‑149 (MQ=255)
                                                                                                             gTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAg                                      <  4:281682/149‑1 (MQ=255)
                                                                                                              tGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAg                                      <  2:516357/148‑1 (MQ=255)
                                                                                                                 tCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACg                                   >  1:790424/1‑148 (MQ=255)
                                                                                                                    tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                               <  3:578830/133‑1 (MQ=255)
                                                                                                                    tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                               >  4:578830/1‑133 (MQ=255)
                                                                                                                    tCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTc                                >  2:617679/1‑148 (MQ=255)
                                                                                                                         tCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCt                           >  2:461128/1‑148 (MQ=255)
                                                                                                                         tCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCt                           >  1:811297/1‑148 (MQ=255)
                                                                                                                            gAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAg                        <  1:344728/148‑1 (MQ=255)
                                                                                                                            gAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAg                        <  1:617679/148‑1 (MQ=255)
                                                                                                                             aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagcagc                                                       <  1:914316/116‑1 (MQ=255)
                                                                                                                             aaTACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagcagc                                                       >  2:914316/1‑116 (MQ=255)
                                                                                                                                  tGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagag                                                                          >  2:520886/1‑92 (MQ=255)
                                                                                                                                  tGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagag                                                                          <  1:520886/92‑1 (MQ=255)
                                                                                                                                    aCAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtc               >  3:873526/1‑149 (MQ=255)
                                                                                                                                        ttcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt           >  4:162972/3‑149 (MQ=255)
                                                                                                                                         gCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt           <  2:675065/148‑1 (MQ=255)
                                                                                                                                          cGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTa                                             >  2:678938/1‑113 (MQ=255)
                                                                                                                                          cGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTa                                             <  1:678938/113‑1 (MQ=255)
                                                                                                                                           gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTggg        >  4:439268/1‑149 (MQ=255)
                                                                                                                                           gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTg          >  4:843868/1‑147 (MQ=255)
                                                                                                                                           gggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTg          <  3:843868/147‑1 (MQ=255)
                                                                                                                                             gagaGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt      <  3:383597/149‑1 (MQ=255)
                                                                                                                                             gagaGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGt       >  1:524496/1‑148 (MQ=255)
                                                                                                                                             gagaGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGt       >  1:881387/1‑148 (MQ=255)
                                                                                                                                                  cGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCAt  >  1:334284/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCAT  >  NC_000913/4269995‑4270288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: