Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,314,227 A→T 6.4% intergenic (+30/+117) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,314,2270AT 6.4% 432.6 / 15.8 125intergenic (+30/+117)yjdP/phnPputative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (55/62);  new base T (4/4);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

Only 100 of 129 total aligned reads displayed.

CGCCGCCAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAA  >  NC_000913/4314081‑4314373
                                                                                                                                                  |                                                                                                                                                  
    gccAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCaa                                                                                                                                              >  3:434252/1‑149 (MQ=255)
     ccAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAAt                                                                                                                                             <  4:312856/149‑1 (MQ=255)
      cAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAAt                                                                                                                                             <  1:634906/148‑1 (MQ=255)
        gTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATggg                                                                                                                                          <  3:268726/149‑1 (MQ=255)
        gTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATgg                                                                                                                                           <  1:523707/148‑1 (MQ=255)
         tttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATggg                                                                                                                                          >  2:96297/1‑148 (MQ=255)
         tttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGACCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATggg                                                                                                                                          <  1:27318/148‑1 (MQ=255)
            gAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                      >  4:186068/1‑149 (MQ=255)
            gAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                      <  3:723808/149‑1 (MQ=255)
             aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtga                                                                                                                                      <  1:546590/148‑1 (MQ=255)
             aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagag                                                                                                                                     <  4:866319/149‑1 (MQ=255)
             aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                      <  2:755702/148‑1 (MQ=255)
              aGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagagg                                                                                                                                    <  3:705835/149‑1 (MQ=255)
               gatagtcgcAGGCAGCTGGACGATCGACAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGTGGATGAAGAGCGGAGAATGAAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                   >  4:772324/7‑149 (MQ=255)
                aTCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                   <  2:145133/148‑1 (MQ=255)
                 tCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggga                                                                                                                                  >  1:497933/1‑148 (MQ=255)
                  cgccgcAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagagggaga                                                                                                                                <  3:7662/149‑1 (MQ=255)
                      gcaggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGaaaa                                                                                                                             <  2:832419/148‑1 (MQ=255)
                        aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACg                                                                                                                           <  2:497933/148‑1 (MQ=255)
                        aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                          <  4:514101/149‑1 (MQ=255)
                         ggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAg                                                                                                                         <  3:731218/149‑1 (MQ=255)
                           cagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAgcg                                                                                                                       >  4:889289/1‑149 (MQ=255)
                                 gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAata                                                                                                                  >  1:243855/1‑148 (MQ=255)
                                 gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGAAGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                            <  3:617389/138‑1 (MQ=255)
                                 gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATCAAGAGCGGAGAATGGAAGATGAGTATGGGCGGTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGAAGAGGGAGAAAACGAGCGCAatat                                                                                                                 <  3:745834/149‑1 (MQ=255)
                                    gATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                              >  3:449734/1‑149 (MQ=255)
                                     aTCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                              >  2:1749/1‑148 (MQ=255)
                                     aTCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                              >  2:343875/1‑148 (MQ=255)
                                              gccagcTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGca                                                                                                    >  4:51594/1‑149 (MQ=255)
                                                 agcTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGcaca                                                                                                  >  2:366500/1‑148 (MQ=255)
                                                  gcTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAAt                                                                                                >  4:467046/1‑149 (MQ=255)
                                                       aTCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGt                                                                                            <  1:124073/148‑1 (MQ=255)
                                                        tCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTcc                                                                                          >  4:435926/1‑149 (MQ=255)
                                                            gAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggggc                                                                                                                                 <  3:65618/106‑3 (MQ=255)
                                                            gAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggggc                                                                                                                                 >  4:65618/1‑104 (MQ=255)
                                                              gCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGc                                                                                    >  3:659493/1‑149 (MQ=255)
                                                                       aCTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTggg                                                                           <  3:186068/149‑1 (MQ=255)
                                                                          ggaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                         >  1:80898/1‑148 (MQ=255)
                                                                           gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                               <  3:461750/125‑1 (MQ=255)
                                                                            aggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                      <  4:673028/149‑1 (MQ=255)
                                                                             ggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                     <  4:153892/149‑1 (MQ=255)
                                                                              gaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                     <  2:132948/148‑1 (MQ=255)
                                                                               aTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                      <  1:380141/146‑1 (MQ=255)
                                                                               aTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                      >  2:380141/1‑146 (MQ=255)
                                                                                  aaGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                 >  2:444496/1‑148 (MQ=255)
                                                                                  aaGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                <  3:558140/149‑1 (MQ=255)
                                                                                  aaGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                <  3:422226/149‑1 (MQ=255)
                                                                                   agagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                               <  4:39838/149‑1 (MQ=255)
                                                                                   agagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                               <  3:52928/149‑1 (MQ=255)
                                                                                    gagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                              >  1:121826/1‑101 (MQ=255)
                                                                                    gagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                              <  2:121826/101‑1 (MQ=255)
                                                                                     agCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                              >  1:93639/1‑148 (MQ=255)
                                                                                       cGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                            >  1:584994/1‑148 (MQ=255)
                                                                                        ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                          <  4:449734/149‑1 (MQ=255)
                                                                                             aTGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                     <  3:335814/149‑1 (MQ=255)
                                                                                              tGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                     >  2:656167/1‑148 (MQ=255)
                                                                                                gAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAg                                                  >  4:768295/1‑149 (MQ=255)
                                                                                                   gATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                     <  3:198372/127‑1 (MQ=255)
                                                                                                      gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAg                                                                                                      <  4:735082/91‑1 (MQ=255)
                                                                                                      gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                          >  1:823436/1‑135 (MQ=255)
                                                                                                      gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                          <  2:823436/135‑1 (MQ=255)
                                                                                                        gTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                               <  3:198846/96‑1 (MQ=255)
                                                                                                        gTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAACGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                               >  4:198846/1‑96 (MQ=255)
                                                                                                         tATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACAt                                         >  4:862379/1‑149 (MQ=255)
                                                                                                            gggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTcc                                      >  4:742779/1‑149 (MQ=255)
                                                                                                              gCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                    >  4:127153/1‑149 (MQ=255)
                                                                                                                gATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                       >  3:367293/1‑128 (MQ=255)
                                                                                                                gATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                       <  4:367293/128‑1 (MQ=255)
                                                                                                                 aTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCtctc                                 <  4:601262/149‑1 (MQ=255)
                                                                                                                     ggTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcatc                              >  1:620843/1‑148 (MQ=255)
                                                                                                                      gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcatca                             >  1:153485/1‑148 (MQ=255)
                                                                                                                        tGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                         <  2:43434/102‑1 (MQ=255)
                                                                                                                         gggTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGc                          <  2:506147/148‑1 (MQ=255)
                                                                                                                          ggTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGcc                         >  1:371864/1‑148 (MQ=255)
                                                                                                                          ggTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACACTCTCTCTCATCACGcc                         >  1:372932/1‑148 (MQ=255)
                                                                                                                           gTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                    <  4:430998/136‑1 (MQ=255)
                                                                                                                               ggAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCa                   >  4:350521/1‑149 (MQ=255)
                                                                                                                                gAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                   >  3:594083/1‑100 (MQ=255)
                                                                                                                                gAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                   <  4:594083/100‑1 (MQ=255)
                                                                                                                                 aGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCaa                  <  2:243855/148‑1 (MQ=255)
                                                                                                                                  gCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAt                 <  1:444496/148‑1 (MQ=255)
                                                                                                                                   cAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtc                >  2:59390/1‑148 (MQ=255)
                                                                                                                                    aGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtctc              >  4:455365/1‑149 (MQ=255)
                                                                                                                                     ggCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtctc              <  1:366500/148‑1 (MQ=255)
                                                                                                                                      gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAata                                                                                                           <  4:484339/54‑1 (MQ=255)
                                                                                                                                      gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAata                                                                                                           >  3:484339/1‑54 (MQ=255)
                                                                                                                                      gCATCACCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTcca            >  3:280338/1‑149 (MQ=255)
                                                                                                                                        aTCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatc          >  4:565216/1‑149 (MQ=255)
                                                                                                                                          cGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcc         >  1:254398/1‑148 (MQ=255)
                                                                                                                                            tctctctCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGCCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat      >  4:69312/4‑149 (MQ=38)
                                                                                                                                            ccccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat      <  3:742779/149‑1 (MQ=255)
                                                                                                                                             cccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                    <  4:119364/118‑1 (MQ=255)
                                                                                                                                             cccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                    >  3:119364/1‑118 (MQ=255)
                                                                                                                                              ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa    >  3:391309/1‑149 (MQ=255)
                                                                                                                                              ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa    >  4:291746/1‑149 (MQ=255)
                                                                                                                                               ctctCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa    >  1:142932/1‑148 (MQ=255)
                                                                                                                                               cTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTc                                                                                      <  3:323504/66‑1 (MQ=255)
                                                                                                                                                tCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCaa   >  2:208526/1‑148 (MQ=255)
                                                                                                                                                 cACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCaaa  <  2:93639/148‑1 (MQ=255)
                                                                                                                                                  aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                    <  3:544278/81‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGCCGCCAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAA  >  NC_000913/4314081‑4314373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: