Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,314,233 A→T 9.0% intergenic (+36/+111) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,314,2330AT 9.0% 431.7 / 26.5 133intergenic (+36/+111)yjdP/phnPputative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (57/64);  new base T (7/5);  total (64/69)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 136 total aligned reads displayed.

GCCAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACC  >  NC_000913/4314085‑4314380
                                                                                                                                                    |                                                                                                                                                   
gccAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCaa                                                                                                                                                     >  3:434252/1‑149 (MQ=255)
 ccAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAAt                                                                                                                                                    <  4:312856/149‑1 (MQ=255)
  cAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGA‑‑GGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATgg                                                                                                                                                  <  2:103933/148‑1 (MQ=255)
    gTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATgg                                                                                                                                                  <  1:523707/148‑1 (MQ=255)
     tttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATgggg                                                                                                                                                <  4:434252/149‑1 (MQ=255)
     tttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATggg                                                                                                                                                 >  2:96297/1‑148 (MQ=255)
     tttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGACCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATggg                                                                                                                                                 <  1:27318/148‑1 (MQ=255)
        gAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                             >  4:186068/1‑149 (MQ=255)
        gAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                             <  3:723808/149‑1 (MQ=255)
         aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtga                                                                                                                                             <  1:546590/148‑1 (MQ=255)
         aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagag                                                                                                                                            <  4:866319/149‑1 (MQ=255)
         aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                             <  2:755702/148‑1 (MQ=255)
          aGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgagg                                                                                                                                           <  3:506323/149‑1 (MQ=255)
          aGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagagg                                                                                                                                           <  3:705835/149‑1 (MQ=255)
           gatagtcgcAGGCAGCTGGACGATCGACAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGTGGATGAAGAGCGGAGAATGAAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                          >  4:772324/7‑149 (MQ=255)
            aTCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                          <  2:194316/148‑1 (MQ=255)
             tCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggga                                                                                                                                         >  1:497933/1‑148 (MQ=255)
                  gcaggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGaaaa                                                                                                                                    <  2:832419/148‑1 (MQ=255)
                    aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACg                                                                                                                                  <  2:497933/148‑1 (MQ=255)
                    aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                 <  4:514101/149‑1 (MQ=255)
                       cagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAgcg                                                                                                                              >  4:889289/1‑149 (MQ=255)
                             gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                                   >  4:617389/1‑138 (MQ=255)
                             gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAata                                                                                                                         >  1:243855/1‑148 (MQ=255)
                             gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGAAGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                                   <  3:617389/138‑1 (MQ=255)
                             gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATCAAGAGCGGAGAATGGAAGATGAGTATGGGCGGTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGAAGAGGGAGAAAACGAGCGCAatat                                                                                                                        <  3:745834/149‑1 (MQ=255)
                                gATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                                     >  3:449734/1‑149 (MQ=255)
                                 aTCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                                     >  2:1749/1‑148 (MQ=255)
                                 aTCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                                     >  2:343875/1‑148 (MQ=255)
                                                ttGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCg                                                                                                     <  3:889289/149‑1 (MQ=255)
                                                   aTCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGt                                                                                                   <  1:124073/148‑1 (MQ=255)
                                                        gAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggggc                                                                                                                                        <  3:65618/106‑3 (MQ=255)
                                                        gAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggggc                                                                                                                                        >  4:65618/1‑104 (MQ=255)
                                                          gCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGc                                                                                           >  3:659493/1‑149 (MQ=255)
                                                            gTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGccc                                                                                         >  3:224608/1‑149 (MQ=255)
                                                                   aCTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                   <  1:1749/148‑1 (MQ=255)
                                                                   aCTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                   <  1:343875/148‑1 (MQ=255)
                                                                      ggaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                                >  1:80898/1‑148 (MQ=255)
                                                                       gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                                      >  4:461750/1‑125 (MQ=255)
                                                                        aggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgaga                                                                              <  2:823576/148‑1 (MQ=255)
                                                                          gaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                           >  3:569572/1‑149 (MQ=255)
                                                                          gaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                            <  2:132948/148‑1 (MQ=255)
                                                                           aTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                             >  2:380141/1‑146 (MQ=255)
                                                                           aTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                             <  1:380141/146‑1 (MQ=255)
                                                                              aaGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                        >  2:444496/1‑148 (MQ=255)
                                                                              aaGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                       <  3:558140/149‑1 (MQ=255)
                                                                                gagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                                     >  1:121826/1‑101 (MQ=255)
                                                                                gagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCa                                                                                                                     <  2:121826/101‑1 (MQ=255)
                                                                                   cGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                   >  1:584994/1‑148 (MQ=255)
                                                                                    ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                 <  4:449734/149‑1 (MQ=255)
                                                                                         aTGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                            <  3:335814/149‑1 (MQ=255)
                                                                                          tGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                            >  2:656167/1‑148 (MQ=255)
                                                                                            gAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAg                                                         >  4:768295/1‑149 (MQ=255)
                                                                                               gATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                            >  4:198372/1‑127 (MQ=255)
                                                                                               gATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                            <  3:198372/127‑1 (MQ=255)
                                                                                                  gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAg                                                                                                             <  4:735082/91‑1 (MQ=255)
                                                                                                  gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAg                                                                                                             >  3:735082/1‑91 (MQ=255)
                                                                                                  gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                 >  1:823436/1‑135 (MQ=255)
                                                                                                    gTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                                      <  3:198846/96‑1 (MQ=255)
                                                                                                    gTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAACGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                                      >  4:198846/1‑96 (MQ=255)
                                                                                                     tATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGcaca                                                 >  2:354466/1‑148 (MQ=255)
                                                                                                        gggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTc                                              <  1:656167/148‑1 (MQ=255)
                                                                                                          gCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           >  4:127153/1‑149 (MQ=255)
                                                                                                            gATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                              <  4:367293/128‑1 (MQ=255)
                                                                                                             aTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCtctc                                        <  4:601262/149‑1 (MQ=255)
                                                                                                                 ggTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcatc                                     >  1:620843/1‑148 (MQ=255)
                                                                                                                  gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCAc                                   <  3:127153/149‑1 (MQ=255)
                                                                                                                    tGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                                <  2:43434/102‑1 (MQ=255)
                                                                                                                    tGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                                >  1:43434/1‑102 (MQ=255)
                                                                                                                     gggTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGc                                 <  2:506147/148‑1 (MQ=255)
                                                                                                                      ggTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACACTCTCTCTCATCACGcc                                >  1:372932/1‑148 (MQ=255)
                                                                                                                       gTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           <  4:430998/136‑1 (MQ=255)
                                                                                                                       gTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           >  3:430998/1‑136 (MQ=255)
                                                                                                                           ggAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCa                          >  4:350521/1‑149 (MQ=255)
                                                                                                                             aGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCaa                         <  2:243855/148‑1 (MQ=255)
                                                                                                                               cAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtc                       >  2:59390/1‑148 (MQ=255)
                                                                                                                                aGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtctc                     >  4:455365/1‑149 (MQ=255)
                                                                                                                                 ggCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtctc                     <  1:366500/148‑1 (MQ=255)
                                                                                                                                 ggCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTcc                    <  4:736381/149‑1 (MQ=255)
                                                                                                                                  gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAata                                                                                                                  >  3:484339/1‑54 (MQ=255)
                                                                                                                                  gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAata                                                                                                                  <  4:484339/54‑1 (MQ=255)
                                                                                                                                  gCATCACCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTcca                   >  3:280338/1‑149 (MQ=255)
                                                                                                                                    aTCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatc                 >  4:565216/1‑149 (MQ=255)
                                                                                                                                      cGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcc                >  1:254398/1‑148 (MQ=255)
                                                                                                                                        tctctctCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGCCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat             >  4:69312/4‑149 (MQ=38)
                                                                                                                                        tcccTCTCCCCATTGGGGTGAGGGGGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat             >  4:842459/2‑149 (MQ=255)
                                                                                                                                        ccccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat             <  3:742779/149‑1 (MQ=255)
                                                                                                                                         cccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           <  4:119364/118‑1 (MQ=255)
                                                                                                                                         cccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           >  3:119364/1‑118 (MQ=255)
                                                                                                                                         cccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat             <  2:620843/148‑1 (MQ=255)
                                                                                                                                          ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa           >  3:391309/1‑149 (MQ=255)
                                                                                                                                          ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa           >  4:291746/1‑149 (MQ=255)
                                                                                                                                           ctctCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa           >  1:142932/1‑148 (MQ=255)
                                                                                                                                           cTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTc                                                                                             <  3:323504/66‑1 (MQ=255)
                                                                                                                                              tccccATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTCAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           <  3:415804/112‑1 (MQ=38)
                                                                                                                                              tccccATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTCAGGGTGAGGGGGCCAGCGCGCACATTCCct                                           >  4:415804/2‑113 (MQ=255)
                                                                                                                                              aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                           >  4:544278/1‑81 (MQ=255)
                                                                                                                                              aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                           <  3:544278/81‑1 (MQ=255)
                                                                                                                                                cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAcccc     <  3:512160/149‑1 (MQ=255)
                                                                                                                                                cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGAGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAcccc     >  4:512160/1‑149 (MQ=255)
                                                                                                                                                  cAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCAc   >  4:389019/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCAGTTTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACC  >  NC_000913/4314085‑4314380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: