Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,377,791 A→T 10.9% intergenic (‑69/+20) blc ← / ← ampC outer membrane lipoprotein cell division and growth lipocalin/penicillin‑binding protein; beta‑lactamase, intrinsically weak

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,377,7910AT10.9% 288.2 / 19.1 91intergenic (‑69/+20)blc/ampCouter membrane lipoprotein cell division and growth lipocalin/penicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base A (35/46);  new base T (4/6);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCTACCAAATC  >  NC_000913/4377646‑4377935
                                                                                                                                                 |                                                                                                                                                
cgcgGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAAt                                                                                                                                                <  2:819571/148‑1 (MQ=255)
 gcggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAAtt                                                                                                                                               <  2:604764/148‑1 (MQ=255)
 gcggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAAtt                                                                                                                                               <  1:197800/148‑1 (MQ=255)
 gcggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTc                                                                                                                                              <  3:182188/149‑1 (MQ=255)
  cggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTTTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCg                                                                                                                                             <  4:64138/149‑1 (MQ=255)
  cggcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTc                                                                                                                                              <  1:326594/148‑1 (MQ=255)
    gcggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCgg                                                                                                                                            <  1:590043/148‑1 (MQ=255)
     aggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTATGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGa                                                                                                                                           <  2:710989/147‑1 (MQ=255)
      ggcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCCGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGAcc                                                                                                                                         <  4:637882/149‑1 (MQ=255)
       gcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcct                                                                                                                                        <  3:238513/149‑2 (MQ=255)
       gcgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcct                                                                                                                                        <  4:236694/149‑2 (MQ=255)
        cgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcct                                                                                                                                        <  2:134353/148‑2 (MQ=255)
        cgTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCg                                                                                                                                       >  3:197434/1‑149 (MQ=255)
         gTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCg                                                                                                                                       >  1:246962/1‑148 (MQ=255)
         gTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCg                                                                                                                                       <  2:556312/148‑1 (MQ=255)
             gagaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATgg                                                                                                                                   >  1:72777/1‑148 (MQ=255)
              agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGaa                                                                                                                                 <  3:13635/149‑1 (MQ=255)
               gaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGaa                                                                                                                                 >  1:43083/1‑148 (MQ=255)
                 aCTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAAGGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGAAAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAtt                                                                                                                               <  2:705785/148‑1 (MQ=255)
                  cTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAtttt                                                                                                                             >  4:600583/1‑149 (MQ=255)
                   tGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTa                                                                                                                            >  4:176482/1‑149 (MQ=255)
                    gCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTa                                                                                                                            >  2:885979/1‑148 (MQ=255)
                     cAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTAc                                                                                                                           <  1:792847/148‑1 (MQ=255)
                        gCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGt                                                                                                                        <  1:763076/148‑1 (MQ=255)
                         cAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGAt                                                                                                                                     >  2:571956/1‑134 (MQ=255)
                         cAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGAt                                                                                                                                     <  1:571956/134‑1 (MQ=255)
                          aaCGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTaga                                                                                                                     >  4:549109/1‑149 (MQ=255)
                          aaCGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTaga                                                                                                                     >  3:322847/1‑149 (MQ=255)
                           aCGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTaga                                                                                                                     >  2:756026/1‑148 (MQ=255)
                             gACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCg                                                                                                                  >  4:396183/1‑149 (MQ=255)
                               ccAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGtt                                                                                                                <  3:396183/149‑1 (MQ=255)
                               ccAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGtt                                                                                                                <  3:301583/149‑1 (MQ=255)
                                 aGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTa                                                                                                               >  2:780522/1‑148 (MQ=255)
                                      tGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAAt                                                                                                          <  1:756026/148‑1 (MQ=255)
                                       gCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATc                                                                                                         <  1:902641/148‑1 (MQ=255)
                                         aGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTg                                                                                                       <  1:885979/148‑1 (MQ=255)
                                         aGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGc                                                                                                      <  4:757820/149‑1 (MQ=255)
                                          gCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGcc                                                                                                     >  3:92628/1‑149 (MQ=255)
                                            tGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCa                                                                                                    <  2:246962/148‑1 (MQ=255)
                                            tGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAg                                                                                                   <  4:197434/149‑1 (MQ=255)
                                                 cTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAggcgg                                                                                               >  1:275563/1‑148 (MQ=255)
                                                  tGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAggcggc                                                                                              >  1:72499/1‑148 (MQ=255)
                                                     ggCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCg                                                                                          >  3:391590/1‑149 (MQ=255)
                                                        aaCGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcctt                                                                                                                                       <  4:364616/101‑3 (MQ=255)
                                                        aaCGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcctt                                                                                                                                       >  3:364616/1‑99 (MQ=255)
                                                         aCGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGAct                                                                                       >  2:39098/1‑148 (MQ=255)
                                                         aCGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGAct                                                                                       >  1:274273/1‑148 (MQ=255)
                                                           gagaGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcctt                                                                                                                                       <  3:340638/98‑3 (MQ=255)
                                                           gagaGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAATTTTCGGAcctt                                                                                                                                       >  4:340638/1‑96 (MQ=255)
                                                              agGGAGTAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCCCt                                                                                 <  3:600583/149‑1 (MQ=255)
                                                                 gAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg                                                                               >  2:695294/1‑148 (MQ=255)
                                                                     aGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTg                                                                           <  1:126344/148‑1 (MQ=255)
                                                                     aGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTg                                                                           <  2:87884/148‑1 (MQ=255)
                                                                     aGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTg                                                                           <  2:455668/148‑1 (MQ=255)
                                                                      ggCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTggg                                                                         <  3:549109/149‑1 (MQ=255)
                                                                      ggCGCATAAATGTATCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTggg                                                                         <  4:572360/149‑1 (MQ=255)
                                                                       gcgcATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGa                                                                        >  4:736014/1‑149 (MQ=255)
                                                                         gcATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGAt                                                                       <  1:780522/148‑1 (MQ=255)
                                                                            tAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTa                                                                                                               >  2:425152/1‑105 (MQ=255)
                                                                            tAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTa                                                                                                               <  1:425152/105‑1 (MQ=255)
                                                                            tAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGc                                                                                                      <  4:315342/114‑1 (MQ=255)
                                                                            tAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGc                                                                                                      >  3:315342/1‑114 (MQ=255)
                                                                            tAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGt                                                                    <  1:541122/148‑1 (MQ=255)
                                                                                 gTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgt                                                              <  4:160921/149‑1 (MQ=255)
                                                                                   ttCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgttt                                                            <  4:391590/149‑1 (MQ=255)
                                                                                   ttCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgttt                                                            <  4:92628/149‑1 (MQ=255)
                                                                                   ttCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtt                                                             <  2:93847/148‑1 (MQ=255)
                                                                                      cTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGc                                                          >  2:175394/1‑148 (MQ=255)
                                                                                       ttACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGcc                                                         <  1:543168/148‑1 (MQ=255)
                                                                                           tGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCAt                                                    >  3:326378/1‑149 (MQ=255)
                                                                                            ggTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCAt                                                    >  1:171805/1‑148 (MQ=255)
                                                                                                tttttCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACg                                                <  2:72777/148‑1 (MQ=255)
                                                                                                          tgtAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGActc                                                                                      >  4:255524/1‑100 (MQ=255)
                                                                                                          tgtAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGActc                                                                                      <  3:255524/100‑1 (MQ=255)
                                                                                                          tgtAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGctc                                     >  4:83463/1‑149 (MQ=255)
                                                                                                           gtAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGctc                                     >  1:145760/1‑148 (MQ=255)
                                                                                                            tAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCtt                                   >  4:580640/1‑149 (MQ=255)
                                                                                                             aGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCtt                                   <  2:72499/148‑1 (MQ=255)
                                                                                                              gATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTc                                                                                                                                              <  2:793847/40‑1 (MQ=255)
                                                                                                              gATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTc                                                                                                                                              >  1:793847/1‑40 (MQ=255)
                                                                                                                 gACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTc                               <  2:752337/148‑1 (MQ=255)
                                                                                                                 gACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTc                               <  1:695294/148‑1 (MQ=255)
                                                                                                                  aCAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCt                              <  2:171805/148‑1 (MQ=255)
                                                                                                                    aGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTgg                            >  2:550993/1‑148 (MQ=255)
                                                                                                                                    gaAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCtttt                                 >  2:874103/1‑127 (MQ=255)
                                                                                                                                    gaAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCtttt                                 <  1:874103/127‑1 (MQ=255)
                                                                                                                                     atcgggTCCGAATTTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGc           >  1:133070/5‑148 (MQ=255)
                                                                                                                                     aaaaGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCt          <  3:580640/149‑1 (MQ=255)
                                                                                                                                        gggcccGAATTTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCTAcc       >  4:523185/5‑149 (MQ=255)
                                                                                                                                         ggTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCTAcc       >  1:160415/1‑148 (MQ=255)
                                                                                                                                              gAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCTTCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCTACCAAATc  >  2:256231/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCGGCGGCGTAGGAGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATAAACGCGACATAGCTACCAAATC  >  NC_000913/4377646‑4377935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑