Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,311 A→G 5.8% intergenic (+87/+27) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3110AG 5.8% 328.5 / 7.6 103intergenic (+87/+27)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base A (54/43);  new base G (3/3);  total (57/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 106 total aligned reads displayed.

AATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGC  >  NC_000913/4519163‑4519457
                                                                                                                                                    |                                                                                                                                                  
aaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCata                                                                                                                                                    >  4:601035/1‑149 (MQ=255)
 aTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCatat                                                                                                                                                   >  3:906242/1‑149 (MQ=255)
   cGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCt                                                                                                                                                 <  4:906242/149‑1 (MQ=255)
     gTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTAc                                                                                                                                               >  4:266082/1‑149 (MQ=255)
      tGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACa                                                                                                                                              >  3:156744/1‑149 (MQ=255)
         aGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATg                                                                                                                                            <  2:809984/148‑1 (MQ=255)
           ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATgggg                                                                                                                                         >  4:430738/1‑149 (MQ=255)
             gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGc                                                                                                                                        <  1:246541/148‑1 (MQ=255)
               aTGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACAt                                                                                                                                             >  2:800998/1‑141 (MQ=255)
               aTGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACAt                                                                                                                                             <  1:800998/141‑1 (MQ=255)
                tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgtt                                                                                                                                    >  3:240490/1‑149 (MQ=255)
                tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                     >  2:658831/1‑148 (MQ=255)
                  aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgt                                                                                                                                  <  4:602690/149‑1 (MQ=255)
                  aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgt                                                                                                                                  <  4:173144/149‑1 (MQ=255)
                   cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGTCCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAGATCTACATGGGGCAgttgt                                                                                                                                  <  2:903138/148‑1 (MQ=255)
                    aaGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAggatttt                                                                                                                                <  4:54935/149‑7 (MQ=37)
                            cGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                        <  4:705720/149‑1 (MQ=255)
                            cGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                        <  4:268569/149‑1 (MQ=255)
                            cGGGCATTATCTGAACATAAAACACTATCAGTAAGCTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                        <  3:617036/149‑1 (MQ=255)
                             gggCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCAGTACCGACCATGTTTATTTCATACATTGTGGGTACTGTTCTTATTATCGCTGCTCATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTGTa                                                                                                                       <  4:561604/149‑1 (MQ=38)
                              ggCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                      >  3:830777/1‑149 (MQ=255)
                              ggCATTATCTGAAAATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTa                                                                                                                       >  1:684292/1‑148 (MQ=255)
                                 aTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                    <  1:658831/148‑1 (MQ=255)
                                 aTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                    <  1:59446/148‑1 (MQ=255)
                                      cTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTaa                                                                                                              >  3:816295/1‑149 (MQ=255)
                                      cTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCATGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTa                                                                                                               >  1:266390/1‑148 (MQ=255)
                                       tGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAt                                                                                                             <  3:430738/149‑1 (MQ=255)
                                         aaCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                            >  1:475470/1‑148 (MQ=255)
                                         aaCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATCGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTc                                                                                                           <  4:892598/149‑1 (MQ=255)
                                            aTAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                        >  3:134340/1‑149 (MQ=255)
                                               aaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACg                                                                                                      >  2:402071/1‑148 (MQ=255)
                                                   acTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGc                                                                                                 >  3:538113/1‑149 (MQ=255)
                                                      aTCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaa                                                                                               >  2:442049/1‑148 (MQ=255)
                                                        cAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCcaaa                                                                                             <  2:408016/148‑1 (MQ=255)
                                                        cAGTAAGTTCGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTGCATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaaaa                                                                                             <  2:752333/148‑1 (MQ=255)
                                                           tAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAATGTGGTAATTCACACGCCAGCAAAAActc                                                                                         >  4:201434/1‑149 (MQ=255)
                                                             aGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTg                                                                                       <  4:203458/149‑1 (MQ=255)
                                                              gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                            >  3:722965/1‑127 (MQ=255)
                                                              gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                            <  4:722965/127‑1 (MQ=255)
                                                                 ggAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCg                                                                                    <  1:402071/148‑1 (MQ=255)
                                                                   aGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtt                                                                                                                          <  1:254362/108‑1 (MQ=255)
                                                                   aGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtt                                                                                                                          >  2:254362/1‑108 (MQ=255)
                                                                   aGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                 >  3:666138/1‑149 (MQ=255)
                                                                   aGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                 <  4:156744/149‑1 (MQ=255)
                                                                    gTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTcc                                                                                <  4:830777/149‑1 (MQ=255)
                                                                    gTCATTACCAACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                 >  2:708384/1‑148 (MQ=255)
                                                                         tACCGAGCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtca                                                                            >  2:494065/1‑148 (MQ=255)
                                                                          aCCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatc                                                                          <  3:601035/149‑1 (MQ=255)
                                                                             gACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaac                                                                       <  4:103387/149‑1 (MQ=255)
                                                                              aCCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTa                                                                                                                       >  3:272915/1‑100 (MQ=255)
                                                                              aCCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTa                                                                                                                       <  4:272915/100‑1 (MQ=255)
                                                                               ccATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaaca                                                                      >  2:458814/1‑148 (MQ=255)
                                                                                cATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaacaa                                                                     <  1:22062/148‑1 (MQ=255)
                                                                                cATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAAt                                                                    >  4:347220/1‑149 (MQ=255)
                                                                                 aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAAt                                                                    >  1:500584/1‑148 (MQ=255)
                                                                                 aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATc                                                                   >  3:759096/1‑149 (MQ=255)
                                                                                 aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATc                                                                   <  4:134340/149‑1 (MQ=255)
                                                                                   gTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAg                                                                 <  3:266082/149‑1 (MQ=255)
                                                                                    tttATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                                >  3:371777/1‑149 (MQ=255)
                                                                                    tttATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAg                                                                 >  1:751876/1‑148 (MQ=255)
                                                                                         ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGt                                                           <  4:240490/149‑1 (MQ=255)
                                                                                           cATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                          <  2:475470/148‑1 (MQ=255)
                                                                                             tACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAc                                                        >  2:446846/1‑148 (MQ=255)
                                                                                                        tATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACa                                                       <  4:476612/138‑1 (MQ=255)
                                                                                                        tATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACa                                                       >  3:476612/1‑138 (MQ=255)
                                                                                                            gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAgg                                        <  3:90536/149‑1 (MQ=255)
                                                                                                             ttCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcat                                                                           <  4:405022/113‑1 (MQ=255)
                                                                                                             ttCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                       >  4:548360/1‑149 (MQ=255)
                                                                                                             ttCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCACCAAAAACTCTGCCGTTCCTtcat                                                                           >  3:405022/1‑113 (MQ=255)
                                                                                                               cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTATTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCa                                     >  4:861764/1‑149 (MQ=255)
                                                                                                                 tattatCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCAc                                    >  1:401214/1‑148 (MQ=255)
                                                                                                                   ttatCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCt                                                                               <  3:866278/103‑1 (MQ=255)
                                                                                                                   ttatCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCt                                                                               >  4:866278/1‑103 (MQ=255)
                                                                                                                        gccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                            <  3:861764/149‑1 (MQ=255)
                                                                                                                         ccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                            >  2:657142/1‑148 (MQ=255)
                                                                                                                           gcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGc                          <  2:401214/148‑1 (MQ=255)
                                                                                                                            cTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGt                        >  4:510661/1‑149 (MQ=255)
                                                                                                                             tAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTc                       >  3:466509/1‑149 (MQ=255)
                                                                                                                               aTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                     <  4:704316/149‑1 (MQ=255)
                                                                                                                                tCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                     <  2:409823/148‑1 (MQ=255)
                                                                                                                                  aaTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGcc                 >  3:98970/1‑149 (MQ=255)
                                                                                                                                   aTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGcc                 >  3:453938/1‑149 (MQ=255)
                                                                                                                                   aTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGc                  >  1:337573/1‑148 (MQ=255)
                                                                                                                                    tAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCct                <  3:590611/149‑1 (MQ=255)
                                                                                                                                       acaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTccc                                              >  3:598529/5‑116 (MQ=255)
                                                                                                                                       acaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTccc                                              <  4:598529/112‑1 (MQ=255)
                                                                                                                                        aTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCttt            >  3:387759/1‑149 (MQ=255)
                                                                                                                                         tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTc           >  4:433037/1‑149 (MQ=255)
                                                                                                                                          acTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtca                                                                            >  1:247354/2‑83 (MQ=38)
                                                                                                                                           cTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc         >  3:696626/1‑149 (MQ=255)
                                                                                                                                            tGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc        >  3:186618/1‑149 (MQ=255)
                                                                                                                                               cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacc     >  4:874162/1‑149 (MQ=255)
                                                                                                                                               cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccac      >  2:880847/1‑148 (MQ=255)
                                                                                                                                                ccATATCTACGTGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca    >  3:56698/1‑149 (MQ=255)
                                                                                                                                                 cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                       >  3:357752/1‑49 (MQ=255)
                                                                                                                                                 cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                       <  4:357752/49‑1 (MQ=255)
                                                                                                                                                 cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca    >  2:118956/1‑148 (MQ=255)
                                                                                                                                                 cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca    >  2:384860/1‑148 (MQ=255)
                                                                                                                                                 cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca    >  1:2712/1‑148 (MQ=255)
                                                                                                                                                  atatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGc  >  4:591741/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGC  >  NC_000913/4519163‑4519457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: