BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,573,189	T→C	5.5%	pseudogene (776/1503 nt)	yjiT →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,189	0	T	C	5.5%	432.6 / 10.5	129	pseudogene (776/1503 nt)	yjiT	pseudogene
Reads supporting (aligned to +/- strand): ref base T (62/59); new base C (5/2); total (68/61)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 134 total aligned reads displayed.

TTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTACGCGCTTT  >  NC_000913/4573041‑4573337

ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                      >  3:404786/1‑149 (MQ=255)

 tAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                      <  1:213120/148‑1 (MQ=255)

 tAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                      >  2:262316/1‑148 (MQ=255)

 tAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                      >  1:413994/1‑148 (MQ=255)

   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                   <  4:630868/149‑1 (MQ=255)

   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTag                                                                                                                                                    <  2:478899/148‑1 (MQ=255)

     acttactCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAgg                                                                                                                                                 <  4:642009/149‑1 (MQ=255)

      cttactCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGc                                                                                                                                                <  3:309777/149‑1 (MQ=255)

           tCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGc                                                                                                                                           >  3:138005/1‑149 (MQ=255)

            cacaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAgg                                                                                                                                                 <  1:194265/142‑1 (MQ=255)

            cacaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAgg                                                                                                                                                 >  2:194265/1‑142 (MQ=255)

            cacaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGcc                                                                                                                                          >  4:529549/1‑149 (MQ=255)

              caACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAACCCt                                                                                                                                         <  2:91801/148‑1 (MQ=255)

                aCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTa                                                                                                                                              >  2:501837/1‑141 (MQ=255)

                aCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCg                                                                                                                                       >  1:298470/1‑148 (MQ=255)

                  cTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGAc                                                                                                                                     >  2:525926/1‑148 (MQ=255)

                   taataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAGACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                                                                   >  3:556355/1‑149 (MQ=255)

                   taataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                                                                   <  4:283088/149‑1 (MQ=255)

                    aataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                   >  2:140590/1‑132 (MQ=255)

                    aataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                   <  1:140590/132‑1 (MQ=255)

                      taaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACaa                                                                                                                                >  4:52157/1‑149 (MQ=255)

                        aaTCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACaaaa                                                                                                                              >  4:421352/1‑149 (MQ=255)

                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                        <  3:584571/149‑1 (MQ=255)

                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                        <  3:450096/149‑1 (MQ=255)

                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                        >  3:627742/1‑149 (MQ=255)

                                aGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                      >  4:346560/1‑149 (MQ=255)

                                 gAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                      <  1:134316/148‑1 (MQ=255)

                                    ccAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTa                                                                                                                   >  2:904096/1‑148 (MQ=255)

                                     cAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTAcg                                                                                                                 >  4:177536/1‑149 (MQ=255)

                                         acaacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGtt                                                                                                             <  3:421352/149‑1 (MQ=255)

                                          caacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAAGGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTg                                                                                                            >  3:596113/1‑149 (MQ=255)

                                           aacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGa                                                                                                                                      <  3:340884/122‑1 (MQ=255)

                                           aacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGa                                                                                                                                      >  4:340884/1‑122 (MQ=255)

                                            acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAag                                                                                                                       <  1:355690/136‑1 (MQ=255)

                                            acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAag                                                                                                                       >  2:355690/1‑136 (MQ=255)

                                            acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTg                                                                                                            <  2:884490/147‑1 (MQ=255)

                                            acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTg                                                                                                            >  1:884490/1‑147 (MQ=255)

                                            acaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGc                                                                                                           >  1:147003/1‑148 (MQ=255)

                                               gCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCaa                                                                                                        >  1:179210/1‑148 (MQ=255)

                                                 ttGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGc                                                                                                           >  3:698464/1‑143 (MQ=255)

                                                 ttGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGc                                                                                                           <  4:698464/143‑1 (MQ=255)

                                                 ttGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAAtt                                                                                                      >  1:373317/1‑148 (MQ=255)

                                                   gagaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCt                                                                                                    <  1:760488/148‑1 (MQ=255)

                                                   gagaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTa                                                                                                   >  4:679039/1‑149 (MQ=255)

                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACCGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAt                                                                                                  <  1:904096/148‑1 (MQ=255)

                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                                                                 >  4:740611/1‑149 (MQ=255)

                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                                                                 <  3:424211/149‑1 (MQ=255)

                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                                                                 <  4:138005/149‑1 (MQ=255)

                                                      aaGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTg                                                                                                <  3:52157/149‑1 (MQ=255)

                                                         gTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTc                                                                                             >  3:760751/1‑149 (MQ=255)

                                                          ttCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTc                                                                                             <  1:623083/148‑1 (MQ=255)

                                                             aCCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGaa                                                                                         >  4:420083/1‑149 (MQ=255)

                                                              cccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                      >  4:409403/1‑119 (MQ=255)

                                                                  aCCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGc                                                                                     <  1:471152/148‑1 (MQ=255)

                                                                       gCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATACGAGTACGCTTTGCCAATTCTATTGGTCAGAAAAGCATCCAg                                                                               >  4:541937/1‑149 (MQ=255)

                                                                         gAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGa                                                                              <  2:413994/148‑1 (MQ=255)

                                                                         gAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGAt                                                                             <  3:679039/149‑1 (MQ=255)

                                                                           aGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAACCATCCAGATGa                                                                           <  3:794172/149‑1 (MQ=255)

                                                                             cGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAg                                                                          <  2:147003/148‑1 (MQ=255)

                                                                              gAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCt                                                                        >  3:549145/1‑149 (MQ=255)

                                                                                 tttCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTa                                                                      >  2:889266/1‑148 (MQ=255)

                                                                                 tttCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAg                                                                     <  3:765123/149‑1 (MQ=255)

                                                                                    ccAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAggg                                                                   >  1:798881/1‑148 (MQ=255)

                                                                                    ccAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGt                                                                  >  3:740453/1‑149 (MQ=255)

                                                                                               aGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTAtctc                                                       >  3:862669/1‑149 (MQ=255)

                                                                                                   gatgaAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTc                                                    <  4:878925/148‑1 (MQ=255)

                                                                                                      gaAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCgg                                                 >  1:48113/1‑148 (MQ=255)

                                                                                                      gaAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGa                                                >  4:364343/1‑149 (MQ=255)

                                                                                                       aaaCAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGa                                                >  2:778744/1‑148 (MQ=255)

                                                                                                          cAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGa                                             <  2:99568/148‑1 (MQ=255)

                                                                                                               aCGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttc                                       >  3:39383/1‑149 (MQ=255)

                                                                                                               aCGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttc                                       <  3:177536/149‑1 (MQ=255)

                                                                                                               aCGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAACAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCCGACGAAGtttc                                       <  3:595798/149‑1 (MQ=255)

                                                                                                                 gCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcct                                     <  4:774096/149‑1 (MQ=255)

                                                                                                                  cATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcct                                     <  1:902341/148‑1 (MQ=255)

                                                                                                                    ttttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttccttt                                   <  1:525926/148‑1 (MQ=255)

                                                                                                                     tttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                      >  4:132156/1‑64 (MQ=255)

                                                                                                                     tttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                  <  1:778744/148‑1 (MQ=255)

                                                                                                                            aTGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTACTGTAAc                           >  1:834379/1‑148 (MQ=255)

                                                                                                                                aCTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTa                                                                      <  2:291604/101‑1 (MQ=255)

                                                                                                                                aCTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCg                      >  3:306249/1‑149 (MQ=255)

                                                                                                                                aCTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCg                      <  4:596113/149‑1 (MQ=255)

                                                                                                                                 cTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCg                      <  2:798881/148‑1 (MQ=255)

                                                                                                                                 cTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCg                      <  2:63877/148‑1 (MQ=255)

                                                                                                                                      aTGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAg                        >  2:176351/1‑141 (MQ=255)

                                                                                                                                        gcttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTc               >  2:880444/5‑148 (MQ=255)

                                                                                                                                         cttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCaa             >  3:247224/4‑149 (MQ=255)

                                                                                                                                          ttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                  <  4:365388/125‑1 (MQ=255)

                                                                                                                                          ttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                  >  3:365388/3‑127 (MQ=255)

                                                                                                                                          ttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAAc            >  3:248815/3‑149 (MQ=255)

                                                                                                                                          gcAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                      <  2:666578/123‑1 (MQ=255)

                                                                                                                                          gcAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                      >  1:666578/1‑123 (MQ=255)

                                                                                                                                             gCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCAt                                                                                   >  2:631139/1‑75 (MQ=255)

                                                                                                                                             gCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCAt                                                                                   <  1:631139/75‑1 (MQ=255)

                                                                                                                                             gCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTa          >  1:765817/1‑148 (MQ=255)

                                                                                                                                               ctctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                      >  4:227185/1‑118 (MQ=255)

                                                                                                                                               ctctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                      <  3:227185/118‑1 (MQ=255)

                                                                                                                                                tctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttcc                                 <  2:770782/122‑1 (MQ=255)

                                                                                                                                                tctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCa              >  2:384604/1‑141 (MQ=255)

                                                                                                                                                tctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTAcgcg      >  3:871814/1‑149 (MQ=255)

TTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTACGCGCTTT  >  NC_000913/4573041‑4573337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`