| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,612,329 | A→G | 9.7% | intergenic (+40/‑82) | ytjA → / → yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,612,329 | 0 | A | G | 9.7% | 153.3 / 6.9 | 52 | intergenic (+40/‑82) | ytjA/yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
| Reads supporting (aligned to +/- strand): ref base A (29/17); new base G (2/3); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTG > NC_000913/4612194‑4612477 | cTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCtt < 2:853067/148‑1 (MQ=255)cTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCtt < 2:814027/148‑1 (MQ=255) gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTg < 1:436426/148‑1 (MQ=255) ggTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGt < 3:562372/149‑1 (MQ=255) cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCt < 2:867384/134‑1 (MQ=255) cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCt > 1:867384/1‑134 (MQ=255) tAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTg > 1:613929/1‑148 (MQ=255) aaTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGt > 1:335373/1‑148 (MQ=255) ttGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTAc > 4:510209/1‑149 (MQ=255) ttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt < 1:26866/148‑1 (MQ=255) ttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt > 2:40732/1‑148 (MQ=255) tcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCt > 2:252309/1‑148 (MQ=255) ggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc > 1:699688/1‑148 (MQ=255) attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCttt > 2:217569/1‑101 (MQ=255) attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcaca > 3:696910/1‑149 (MQ=255) attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCC‑GCTGGACTGGCttt < 1:217569/101‑1 (MQ=255) cTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACtt > 1:303497/1‑128 (MQ=255) cTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACtt < 2:303497/128‑1 (MQ=255) cTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACaaa > 2:857633/1‑148 (MQ=255) ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc < 1:641893/148‑1 (MQ=255) gTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCTAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAgg > 3:263749/1‑149 (MQ=255) gTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg < 2:144729/148‑1 (MQ=255) tGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACaaaaa < 1:614478/140‑1 (MQ=255) tGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACaaaaa > 2:614478/1‑140 (MQ=255) tttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCagag > 4:706814/1‑149 (MQ=255) tttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCaga < 2:335373/148‑1 (MQ=255) ttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCagag > 2:788052/1‑148 (MQ=255) ttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGA‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGAAAAGCagag > 2:50001/1‑148 (MQ=255) aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGc > 4:647233/1‑149 (MQ=255) gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTTCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc > 2:809778/1‑148 (MQ=255) gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc < 1:809778/148‑1 (MQ=255) cGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa < 1:857633/148‑1 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAAc > 2:358300/1‑148 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACg > 4:453824/1‑149 (MQ=255) tAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGc > 4:389977/1‑149 (MQ=255) aTTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTg < 3:510209/149‑1 (MQ=255) aTTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTg < 3:453824/149‑1 (MQ=255) tttCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTAGGGCAGCGAATACCTGTAACGCTTg < 2:613929/148‑1 (MQ=255) ttCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTgg > 1:707115/1‑148 (MQ=255) cATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTg > 4:176931/1‑149 (MQ=255) aaaaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg < 2:872079/109‑1 (MQ=255) aaaaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg > 1:872079/1‑109 (MQ=255) aaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGt < 3:176931/149‑1 (MQ=255) aaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGt > 4:740061/1‑149 (MQ=255) cAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg < 3:196919/98‑1 (MQ=255) cAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg > 4:196919/1‑98 (MQ=255) aaaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc > 3:304427/1‑149 (MQ=255) aaaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGc > 1:337637/1‑148 (MQ=255) aaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc > 2:623032/1‑148 (MQ=255) aaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc > 1:588379/1‑148 (MQ=255) aaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc > 1:33544/1‑148 (MQ=255) cAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTTCAGcc < 1:40732/148‑1 (MQ=255) aGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTg > 3:7999/1‑149 (MQ=255) | CTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTG > NC_000913/4612194‑4612477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |