New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3927418 =153 (0.920)4 (0.030) 3/490 15.4 2.7% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 146 (0.920)coding (303/993 nt) asnA asparagine synthetase A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGGCATCAAGATCCGGATAACGAGACAGTAACTCCTGGCTGTGTACGAAGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3927701‑3927418
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcccttcgcccCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAA  >  NC_000913/3927457‑3927726
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
TGGCATCAAGATCCGGATAACGAGACAGTAACTCCTGGCTGTGTACGAAGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTTCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCCCTGGTCAACATCGACCCAGTGCAACGCAGAAAGACGGTCATCCATCGGCGACAGGGCTTTCATGGCCGTGTACAACCC                                                                                                                                                                                                                                                                                       >  4:504014/1‑277
     TCAAGATCCGGATAACGAGACAGTAACTCCTGGCTGTGTGCGAAGTGGATCTGTTCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGACCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCC                                                                                                                                                                                                                                                                                 <  2:67778/278‑1
      CAAGATCCGGATAACGAGACAGTAACTCCTGGCTGTGTACGAAGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGAGTGTACAGCCCTTCG                                                                                                                                                                                                                                                                                   >  1:125133/1‑275
        cgacCCGGGTAACGAGACAGTACCTCCTGGCTGTGTACGCAGTGGTTCTGATCCGGCAGGAACGGTGCCAGTCCAAACTCTTTGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGCTCGCCTCTACCGTGCTTTTCAGAGTCGAGAAGTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCACCATAGGCCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCC                                                                                                                                                                                                                                                                                 <  3:913047/271‑1
                                        GTGTCCGATGGGGACTTGGTCCGGCAGGAACGGTGCCAGGGCAACCTCTTCGCTAACCGAAGCTTCGGTTGCTTTGATTCCCGCCCAGAGCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCGTTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGGGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACT                                                                                                                                                                                                                                                   <  2:352797/273‑1
                                         TGTACGAAGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTCTCCGAGTCGAGAATTTACGTTCACCGTCGCCCATTACGCGTTCCCAGTCCCCCTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCATCATCGGGGCGCAGGTCTTTCATGTGCGCTTACAGCCCTTCGCCCCGATCAACACCGTCTTTCTCCGTTCCCCTCG                                                                                                                                                                                                                                                 >  3:334314/1‑274
                                                AGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTT                                                                                                                                                                                                                                                                                     >  3:288787/1‑231
                                                    GATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCC                                                                                                                                                                                                                                                                                 <  1:288787/231‑1
                                                                  gggACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGAGCGCCTCTACCGTGCTTCTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGAAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGG                                                                                                                                                                                                                        <  1:334314/271‑1
                                                                                                                                                                                            TCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTACTGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGACCGGTAGAGGCGATCTGGTCGGGCATTAAAGACACCGAAGCTGAGGTTAGCGAAGAGTTTGCGCTGGTACtggtc                                                                                              >  3:821424/1‑269
                                                                                                                                                                                                                                                                                     CCTTCGCCCCGATGAAGACCGTCTTTCGCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAACGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGCAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAGCCACTGCGTAACCAGCCAGCAGTTACTGTCGCTTTATCCTGATAGTGATGCCACAGGGCTTGAGCGGGCGAAGGCGA   >  2:600074/1‑278
                                                                                                                                                                                                                                                                                      tTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGGGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAA  <  2:706719/277‑1
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCCGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCTGGTTCTTGATGCAACGGGGGGTGAGGGGGggttgccg    >  3:357403/1‑267
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCG    >  1:360174/1‑275
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCG    >  1:663356/1‑275
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCG    >  1:161633/1‑275
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGAGCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCc    >  1:396981/1‑274
                                                                                                                                                                                                                                                                                       TTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCAAGGAGTTACTGTCTCGTTATCCGGACCTTGACTCCAACGTCCGTGAGCGGGCGATAGCG    >  3:271291/1‑275
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
TGGCATCAAGATCCGGATAACGAGACAGTAACTCCTGGCTGTGTACGAAGTGGATCTGATCCGGCAGGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACTGGTCAACATAGACCGAGTGCAACGGAGAAAGACGGTCTTCATCGGGGCGAAGGGCTTTCATGTGCGTGTACAGCCCTTCGCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3927701‑3927418
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcccttcgcccCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAA  >  NC_000913/3927457‑3927726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.