BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,987,442	Δ1 bp	5.8%	intergenic (+14/+65)	ftnB → / ← yecJ	ferritin B, putative ferrous iron reservoir/DUF2766 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,987,442	0	T	.	5.8%	683.3 / 28.7	190	intergenic (+14/+65)	ftnB/yecJ	ferritin B, putative ferrous iron reservoir/DUF2766 family protein
Reads supporting (aligned to +/- strand): ref base T (86/93); new base . (4/7); total (90/100)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 193 total aligned reads displayed.

CAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAAC  >  NC_000913/1987186‑1987713

                aaTACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCg                                                                                                                                                                                                                                               <  2:33277/275‑1 (MQ=255)

                                        tGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCACTTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGACGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGCTCATAACCGGCTCTAATGCATT‑GCGCCGATCAAGGTTTTGAGAACCCGCTGCCTCATCTGTTTGAagccgc                                                                                                                                                                                                                        >  4:146405/1‑274 (MQ=255)

                                                        tGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCCCACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTTAGAAACCGCTGCCTCCTCTGTTTGAAGCAGCGGTTTTTTTATTGGGAt                                                                                                                                                                                                       >  1:10303/1‑275 (MQ=255)

                                                                   aaCTGAATGATTATTCAACCGTCAATTTCCCGCGCGATCTGGCAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtgtg                                                                                                                                                                                             <  1:48421/274‑1 (MQ=255)

                                                                            aggatTGAACCTTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCGTCGGCGCTAATGCATT‑GCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACt                                                                                                                                                                                    <  4:32161/272‑1 (MQ=255)

                                                                                              tCCTGCGCGATCTGGAAAAAGAACAGCAGCATGAGGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGa                                                                                                                                                                  <  2:99387/274‑1 (MQ=255)

                                                                                                              aaaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTaa                                                                                                                                                                                                                                                                                >  6:270781/1‑149 (MQ=255)

                                                                                                              aaaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTaa                                                                                                                                                                                                                                                                                <  5:851445/149‑1 (MQ=255)

                                                                                                               aaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACTATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTACTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAAt                                                                                                                                                                                                                                                                               <  7:996419/149‑1 (MQ=255)

                                                                                                               aaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAAt                                                                                                                                                                                                                                                                               >  8:1041394/1‑149 (MQ=255)

                                                                                                                aaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGc                                                                                                                                                                                                                                                                             <  7:1018829/149‑1 (MQ=255)

                                                                                                                    aCAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCAtt                                                                                                                                                                                                                                                                          >  5:339238/1‑149 (MQ=255)

                                                                                                                      agcagcATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑gc                                                                                                                                                                                                                                                                       <  8:1190132/149‑1 (MQ=255)

                                                                                                                      agcagcATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑gc                                                                                                                                                                                                                                                                       >  5:1479064/1‑149 (MQ=255)

                                                                                                                       gcagcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑gcg                                                                                                                                                                                                                                                                      >  5:1157340/1‑149 (MQ=255)

                                                                                                                          gcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCATTAGCGCCg                                                                                                                                                                                                                                                                   <  8:971645/149‑1 (MQ=255)

                                                                                                                           caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGa                                                                                                                                                                                                                                                                  <  8:433214/149‑1 (MQ=255)

                                                                                                                           caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGa                                                                                                                                                                                                                                                                  <  7:719143/149‑1 (MQ=255)

                                                                                                                            atgatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGAt                                                                                                                                                                                                                                                                 <  7:336106/149‑1 (MQ=255)

                                                                                                                            atgatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGAGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGAt                                                                                                                                                                                                                                                                 <  5:1461904/149‑1 (MQ=255)

                                                                                                                             tgatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTGTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGTTTGCCCGCATGGTGACCggg                                                                                                                                  >  2:264788/1‑275 (MQ=255)

                                                                                                                                   tCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGtt                                                                                                                                                                                                                                                          >  8:299721/1‑149 (MQ=255)

                                                                                                                                    cTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAAAATGTTCTGAATGTCGTGTCACACCAGGTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGttt                                                                                                                                                                                                                                                         <  6:598420/149‑1 (MQ=255)

                                                                                                                                     tGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGtttt                                                                                                                                                                                                                                                        <  5:951761/149‑1 (MQ=255)

                                                                                                                                           tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGaaa                                                                                                                                                                                                                                                  >  7:309977/1‑149 (MQ=255)

                                                                                                                                           tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGaaa                                                                                                                                                                                                                                                  >  5:363214/1‑149 (MQ=255)

                                                                                                                                            gcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAAc                                                                                                                                                                                                                                                 >  6:192413/1‑149 (MQ=255)

                                                                                                                                               aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGc                                                                                                                                                                                                                                              <  6:58980/149‑1 (MQ=255)

                                                                                                                                                  cATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGc                                                                                                                                                                                                                                           <  8:948230/149‑1 (MQ=255)

                                                                                                                                                   aTTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGcc                                                                                                                                                                                                                                          >  6:346043/1‑149 (MQ=255)

                                                                                                                                                   aTTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGcc                                                                                                                                                                                                                                          >  8:677494/1‑149 (MQ=255)

                                                                                                                                                        tgatgaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATc                                                                                                                                                                                                                                     <  7:1173658/149‑1 (MQ=255)

                                                                                                                                                          atgaAGTGCGGAGTGCGAAACTTGCGGGTATGTGACCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGACGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGt                                                                                                      <  2:149586/274‑1 (MQ=255)

                                                                                                                                                            gaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt                                                                                                                                                                                                                                 <  5:192413/149‑1 (MQ=255)

                                                                                                                                                             aaGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGttt                                                                                                                                                                                                                                <  6:318044/149‑1 (MQ=255)

                                                                                                                                                               gTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGatat                                                                                                >  2:45790/1‑275 (MQ=255)

                                                                                                                                                                tGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                                                                                                                                             <  5:386778/149‑1 (MQ=255)

                                                                                                                                                                         cGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtt                                                                                                                                                                                                                    <  6:363214/149‑1 (MQ=255)

                                                                                                                                                                         cGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                                                                                                                                                 <  2:207368/216‑1 (MQ=255)

                                                                                                                                                                           aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt                                                                                                                                                                                                                  >  7:1084610/1‑149 (MQ=255)

                                                                                                                                                                            aaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttttt                                                                                                                                                                                                                 <  8:406124/149‑1 (MQ=255)

                                                                                                                                                                               ttGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTa                                                                                                                                                                                                              >  5:1358095/1‑149 (MQ=255)

                                                                                                                                                                                tGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTaa                                                                                                                                                                                                             <  7:608912/149‑1 (MQ=255)

                                                                                                                                                                                tGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTaa                                                                                                                                                                                                             <  8:1343986/149‑1 (MQ=255)

                                                                                                                                                                                tGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTaa                                                                                                                                                                                                             <  7:785250/149‑1 (MQ=255)

                                                                                                                                                                                 gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                                                                                                                                            <  7:1195096/149‑1 (MQ=255)

                                                                                                                                                                                     gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                                                                                                                                                                                                        <  6:47472/149‑1 (MQ=255)

                                                                                                                                                                                     gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTc                                                                                                                                                                                                     <  4:364191/152‑1 (MQ=255)

                                                                                                                                                                                          tgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAc                                                                                                                                                                                                   >  6:737619/1‑149 (MQ=255)

                                                                                                                                                                                            cccTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAccc                                                                                                                                                                                                 <  5:270166/149‑1 (MQ=255)

                                                                                                                                                                                                  gCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                                                                                                                                           >  6:787595/1‑149 (MQ=255)

                                                                                                                                                                                                  gCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                                                                                                                                           <  8:1360107/149‑1 (MQ=255)

                                                                                                                                                                                                      accgaccAACATGTTCTGAATGTCGTGTCACACCAGCTTCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGATGatg                                                                                                                                                                                                                                                             <  5:1020458/79‑3 (MQ=25)

                                                                                                                                                                                                      accgaccAACATGTTCTGAATGTCGTGTCACACCAGCTTCATTGATCATCATCGGCGC‑AATGCATTAGCGCCGATGatg                                                                                                                                                                                                                                                             >  6:1020458/1‑77 (MQ=25)

                                                                                                                                                                                                          accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                                                                                                                                           >  7:1259176/1‑141 (MQ=255)

                                                                                                                                                                                                          accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                                                                                                                                           <  8:1259176/141‑1 (MQ=255)

                                                                                                                                                                                                          accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                                                                                                                                                   <  5:22971/149‑1 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGa                                                                                                                                                                                                                                                                  >  6:671611/1‑69 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGa                                                                                                                                                                                                                                                                  <  5:671611/69‑1 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAcc                                                                                                                                                                                                  <  8:884577/133‑1 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAAAGGgcggg                                                    >  2:123747/1‑273 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGAGGGCTTTTTGGATTGCCCGCATGGTGACCGGGTAGGCGGCGGCGGGGTGGGTAGTTAAGTCGAGACGCTTCAGCTGACTGGACCTTTGTTCACGAAGTTCAACgggggac                                                    >  4:245512/1‑271 (MQ=255)

                                                                                                                                                                                                           ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGATTGAAGCAGCGGTTTTTTTAATGGGATTCAcc                                                                                                                                                                                                  >  7:884577/1‑133 (MQ=255)

                                                                                                                                                                                                            cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAg                                                                                                                                                                                                                                                             >  7:840393/1‑73 (MQ=255)

                                                                                                                                                                                                            cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCg                                                                                                                                                                                                                       <  7:948725/111‑1 (MQ=255)

                                                                                                                                                                                                             aaCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                                                                                                                                                >  7:604789/1‑149 (MQ=255)

                                                                                                                                                                                                             aaCATGTTCTGAATGTCGTGGCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                                                                                                                                                <  8:855890/149‑1 (MQ=255)

                                                                                                                                                                                                               cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGtt                                                                                                                                                                              >  5:886477/1‑149 (MQ=255)

                                                                                                                                                                                                                aTGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                                                                                                                                                 <  8:1012209/145‑1 (MQ=255)

                                                                                                                                                                                                                   ttCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAAt                                                                                                                                                                          >  7:1328223/1‑149 (MQ=255)

                                                                                                                                                                                                                   ttCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAAt                                                                                                                                                                          >  5:856868/1‑149 (MQ=255)

                                                                                                                                                                                                                       gAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttttt                                                                                                                                                                                                                >  7:1347821/1‑107 (MQ=255)

                                                                                                                                                                                                                             cGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                                                                                                                                            >  4:359701/1‑121 (MQ=255)

                                                                                                                                                                                                                                 tCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTa                                                                                                                                                                                        <  2:359701/121‑1 (MQ=255)

                                                                                                                                                                                                                                 tCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtt                                                                                                                                                            <  6:1288498/149‑1 (MQ=255)

                                                                                                                                                                                                                                   acacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt                                                                                                                                                          >  7:211254/1‑149 (MQ=255)

                                                                                                                                                                                                                                   acacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt                                                                                                                                                          >  7:485642/1‑149 (MQ=255)

                                                                                                                                                                                                                                    cacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                                                                                                                                                         <  5:305613/149‑1 (MQ=255)

                                                                                                                                                                                                                                    cacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                                                                                                                                                         <  8:621630/149‑1 (MQ=255)

                                                                                                                                                                                                                                    cacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                                                                                                                                                         >  5:1255279/1‑149 (MQ=255)

                                                                                                                                                                                                                                    cacCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                                                                                                                                                         <  7:1435822/149‑1 (MQ=255)

                                                                                                                                                                                                                                     acCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGt                                                                                                                                                                                                                                  >  7:1223317/1‑75 (MQ=255)

                                                                                                                                                                                                                                     acCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATCCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTg                                                                                                                                                        <  5:632797/149‑1 (MQ=255)

                                                                                                                                                                                                                                      ccAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTg                                                                                                                                                        >  8:1400533/1‑148 (MQ=255)

                                                                                                                                                                                                                                       cAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCACCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGa                                                                                                                                                      >  8:732207/1‑149 (MQ=255)

                                                                                                                                                                                                                                           tGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGc                                                                                                                                                  <  7:677494/149‑1 (MQ=255)

                                                                                                                                                                                                                                            gCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                                                                                                                                                 >  6:1164486/1‑149 (MQ=255)

                                                                                                                                                                                                                                             cATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgcgc                                                                                                                                                                    >  7:11208/1‑129 (MQ=255)

                                                                                                                                                                                                                                              aTTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCg                                                                                                                                               >  8:533190/1‑149 (MQ=255)

                                                                                                                                                                                                                                                 gATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCAt                                                                                                                                            >  5:477599/1‑149 (MQ=255)

                                                                                                                                                                                                                                                  atcatcatcGGCGATAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATg                                                                                                                                           >  8:19336/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   tcatcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATgg                                                                                                                                          >  8:345765/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    catcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGt                                                                                                                                         >  5:58773/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    catcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGt                                                                                                                                         <  7:179425/149‑1 (MQ=255)

                                                                                                                                                                                                                                                     atcatcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg                                                                                                                                        >  6:238943/1‑149 (MQ=255)

                                                                                                                                                                                                                                                         tcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGa                                                                                                                                                      <  6:223511/131‑1 (MQ=255)

                                                                                                                                                                                                                                                         tcGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGa                                                                                                                                                      >  5:223511/1‑131 (MQ=255)

                                                                                                                                                                                                                                                          cGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCgg                                                                                                                                   <  7:414364/149‑1 (MQ=255)

                                                                                                                                                                                                                                                             cgcTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCACCGGGCGCGATTACATCGAGACAATCCATAATTTGTTTGATAACCAACTGGCAAGCAAc  >  4:114820/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                tAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACAGCTGCCTAATCTGTTTGAAGCAGCGGTTTTTTTAAAGGGATTCACCCTGTGGGGTAAAATTGAGTTCAATAAGCGCGATGGCTTTCTGGATTGCCCGCATGGTGACCGGGTCTgc                                                                                                                             >  6:1474868/1‑149 (MQ=255)

CAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATT‑GCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAAC  >  NC_000913/1987186‑1987713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`