Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,066,319 Δ1 bp 7.1% coding (855/897 nt) ygfI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,066,3190C. 7.1% 686.2 / 44.9 198coding (855/897 nt)ygfIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (92/90);  new base . (6/8);  total (99/99)
Fisher's exact test for biased strand distribution p-value = 7.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-02

Only 100 of 201 total aligned reads displayed.

AACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCCCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGAAAG  >  NC_000913/3066081‑3066583
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                        
aaCAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCCCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa                                                                                                                                                                                                                                      >  2:339523/1‑275 (MQ=255)
              ggtCTGCACCGTATTTCCCCGCTTCAACGGGCCCGCACTATCATTGATAATTGTGCACATCCTATGAATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCat                                                                                                                                                                                                                        <  4:339523/273‑1 (MQ=255)
               aTCTGCGCGGTCTTTCCCCGCTGCAACGCGCCCGCACTATCATTGATAATTGTGCACAGCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCat                                                                                                                                                                                                                        <  2:75090/274‑1 (MQ=255)
                                                              aaTTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATCTTCACAACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAACGATATTCCTGCATATCACTCGGCCTGTGCGGTGCCTCAAAATATTTCAAACTTGCCGGaat                                                                                                                                                                        >  4:99002/1‑273 (MQ=255)
                                                                ttGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCa                                                                                                                                                                      >  2:224777/1‑275 (MQ=255)
                                                                   tgCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAtt                                                                                                                                                                                                                                            >  2:85785/1‑202 (MQ=255)
                                                                     cacaTCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAt                                                                                                                                                                                                                                             >  4:273485/1‑199 (MQ=255)
                                                                         tCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGaa                                                                                                                                                                                                                                         <  2:273485/199‑1 (MQ=255)
                                                                                         tATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCa                                                                                                                                                        <  2:176982/264‑1 (MQ=255)
                                                                                          aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGc                                                                                                                                                                                                                                                                          <  8:408461/149‑1 (MQ=255)
                                                                                            cTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCAt                                                                                                                                                                                                                                                                        <  5:1168822/149‑1 (MQ=255)
                                                                                             tGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                                                                                                                                                       <  5:1451105/149‑1 (MQ=255)
                                                                                                 tCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                                                                                                                                                                                                   >  8:836389/1‑149 (MQ=255)
                                                                                                 tCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                                                                                                                                                                                                   <  6:561255/149‑1 (MQ=255)
                                                                                                 tCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                                                                                                                                                                                                   >  5:862232/1‑149 (MQ=255)
                                                                                                   gCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                                                                                                                                                                                                   <  4:181821/147‑1 (MQ=255)
                                                                                                     tATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGt                                                                                                                                                                                                                                                               >  7:1138213/1‑149 (MQ=255)
                                                                                                       tCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTgac                                                                                                                                                                                                                                                                  <  5:590847/144‑3 (MQ=38)
                                                                                                       tCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTgac                                                                                                                                                                                                                                                                  >  6:590847/1‑142 (MQ=38)
                                                                                                          ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                                                                                                                                                                                          >  7:1428257/1‑149 (MQ=255)
                                                                                                              aaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgatg                                                                                                                                                                                                                                                      >  7:87051/1‑149 (MQ=255)
                                                                                                                tGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGc                                                                                                                                                                                                                                                                          >  7:157118/1‑127 (MQ=255)
                                                                                                                tGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgatgat                                                                                                                                                                                                                                                    <  8:495107/149‑1 (MQ=255)
                                                                                                                  cgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGAttt                                                                                                                                                                                                                                                  <  7:502010/149‑1 (MQ=255)
                                                                                                                   gcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTc                                                                                                                                                                                                                                                 <  5:343813/149‑1 (MQ=255)
                                                                                                                   gcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTGCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCa                                                                                                                   <  1:39132/275‑1 (MQ=255)
                                                                                                                    ccTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCt                                                                                                                                                                                                                                                <  5:1211299/149‑1 (MQ=255)
                                                                                                                       ggCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAt                                                                                                                                                                                                                                             <  8:250471/149‑1 (MQ=255)
                                                                                                                       ggCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAt                                                                                                                                                                                                                                             <  8:824079/149‑1 (MQ=255)
                                                                                                                           gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGaa                                                                                                                                                                                                                                         <  5:276008/149‑1 (MQ=255)
                                                                                                                           gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGaa                                                                                                                                                                                                                                         >  6:420451/1‑149 (MQ=255)
                                                                                                                               tatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAATCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                                                                                                                                                     <  8:1201969/149‑1 (MQ=255)
                                                                                                                               tatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                                                                                                                                                     <  6:1377297/149‑1 (MQ=255)
                                                                                                                                   caccacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                                                                                                                                                                                                 <  6:1290223/149‑1 (MQ=255)
                                                                                                                                   caccacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                                                                                                                                                                                                 >  8:1439724/1‑149 (MQ=255)
                                                                                                                                    accacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGatat                                                                                                                                                                                                                                >  5:804431/1‑149 (MQ=255)
                                                                                                                                      cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                                                                                                                                                                                              <  6:1205378/149‑1 (MQ=255)
                                                                                                                                          aTCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                                                                                                                                                                                                 <  4:79323/142‑1 (MQ=255)
                                                                                                                                           tCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                                                                                                                                                                                                         >  8:703476/1‑149 (MQ=255)
                                                                                                                                               tgCCACTTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATTCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTa                                                                                       <  1:13573/274‑1 (MQ=255)
                                                                                                                                                 ccACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACa                                                                                                                                                                                                                   >  8:1119268/1‑149 (MQ=255)
                                                                                                                                                  cACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACAt                                                                                                                                                                                                                  >  7:1197153/1‑149 (MQ=255)
                                                                                                                                                   aCGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATc                                                                                                                                                                                                                 <  8:774667/149‑1 (MQ=255)
                                                                                                                                                   aCGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATc                                                                                                                                                                                                                 >  7:1026305/1‑149 (MQ=255)
                                                                                                                                                    cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCg                                                                                                                                                                                                                <  5:1369776/149‑1 (MQ=255)
                                                                                                                                                      tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc                                                                                                                                                                                                              >  5:281651/1‑149 (MQ=255)
                                                                                                                                                       tttCGAATTACACCGTAATTTAATTGCAACCGGCTGTATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGat                                                                               <  4:224777/274‑1 (MQ=255)
                                                                                                                                                        ttCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                                                                                                                                                                                          <  8:181854/103‑1 (MQ=255)
                                                                                                                                                        ttCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                                                                                                                                                    <  5:513733/125‑1 (MQ=255)
                                                                                                                                                        ttCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                                                                                                                                                    >  6:513733/1‑125 (MQ=255)
                                                                                                                                                              ttACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg                                                                                                                                                                                                      >  7:703031/1‑149 (MQ=255)
                                                                                                                                                               tACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGt                                                                                                                                                                                                     <  8:1026305/149‑1 (MQ=255)
                                                                                                                                                                 cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGc                                                                                                                                                                                                   <  7:1247008/149‑1 (MQ=255)
                                                                                                                                                                  acCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCa                                                                                                                                                                                                  <  8:87051/149‑1 (MQ=255)
                                                                                                                                                                   ccGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt                                                                                                                                                                                                 <  6:471865/149‑1 (MQ=255)
                                                                                                                                                                    cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATGAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                                                                                                                                                                                                >  7:960031/1‑149 (MQ=255)
                                                                                                                                                                     gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAtt                                                                                                                                                                                                                                            <  5:1042199/104‑1 (MQ=255)
                                                                                                                                                                        atttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                                                                                                                                                                                            >  8:542/1‑149 (MQ=255)
                                                                                                                                                                        atttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                                                                                                                                                                                            <  7:836389/149‑1 (MQ=255)
                                                                                                                                                                              ttGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTc                                                                                                                                                                                      <  5:420451/149‑1 (MQ=255)
                                                                                                                                                                                      ggCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGc                                                                                                                                                                              <  6:717832/149‑1 (MQ=255)
                                                                                                                                                                                        cTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCg                                                                                                                                                                            >  7:203694/1‑149 (MQ=255)
                                                                                                                                                                                          cGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATAAAAATATTTCAGCGTTGCCGGa                                                                                                                                                                          >  6:367008/1‑149 (MQ=255)
                                                                                                                                                                                             tGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTc                                                                                                                                                                       >  8:156163/1‑149 (MQ=255)
                                                                                                                                                                                                 gggTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCa                                                                                                                                                                   <  5:677088/149‑1 (MQ=255)
                                                                                                                                                                                                       aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGcc                                                                                                                                                             >  7:229568/1‑149 (MQ=255)
                                                                                                                                                                                                       aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGNTGCCGGATTCACCATTCGCCAGGCAAAATGCGGGACCACCGTCAGAAAATGTCCGCGCTCCACGGCGATNATTTTGGCCATAAAACTGAAGGGACGATAAAAAAGGGTTGGGTTGNTNCGAAAAGGGCAGATATTTGcgacaaaac                               >  3:38341/1‑267 (MQ=255)
                                                                                                                                                                                                        tGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa                                                                                                                                                            <  6:1390620/149‑1 (MQ=255)
                                                                                                                                                                                                         gACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAg                                                                                                                                                           >  7:786683/1‑149 (MQ=255)
                                                                                                                                                                                                         gACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAg                                                                                                                                                           >  8:74831/1‑149 (MQ=255)
                                                                                                                                                                                                           cTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGc                                                                                                                                                         <  5:478513/149‑1 (MQ=255)
                                                                                                                                                                                                             cGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                                                                                                                                                                                              <  5:196000/78‑1 (MQ=255)
                                                                                                                                                                                                              gTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCaaa                                                                                                                                                      >  7:563103/1‑149 (MQ=255)
                                                                                                                                                                                                               tCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCaaaa                                                                                                                                                     >  7:476842/1‑149 (MQ=255)
                                                                                                                                                                                                                cccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                                                                                                                                                     >  6:1058480/1‑68 (MQ=255)
                                                                                                                                                                                                                  cGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAc                                                                                                                                                                     >  6:155330/1‑130 (MQ=255)
                                                                                                                                                                                                                  cGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGc                                                                                                                                                  >  6:1102613/1‑149 (MQ=255)
                                                                                                                                                                                                                   gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                                                                                                                                                 <  7:872428/149‑1 (MQ=255)
                                                                                                                                                                                                                   gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                                                                                                                                                 <  8:1155174/149‑1 (MQ=255)
                                                                                                                                                                                                                   gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                                                                                                                                                 <  6:281651/149‑1 (MQ=255)
                                                                                                                                                                                                                   gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGCTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                                                                                                                                                 >  6:586857/1‑149 (MQ=255)
                                                                                                                                                                                                                    tCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATc                                                                                                                                                                                                                 <  5:1306297/84‑1 (MQ=255)
                                                                                                                                                                                                                    ccACGATGCTTCAGCATATTGCTGAAGATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCgg                                                                                                                                                >  5:1100113/2‑149 (MQ=255)
                                                                                                                                                                                                                     cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCggg                                                                                                                                               <  8:1428257/149‑1 (MQ=255)
                                                                                                                                                                                                                        gatgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATc                                                                                                                                            >  8:369826/5‑149 (MQ=255)
                                                                                                                                                                                                                        gATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATc                                                                                                                                            <  7:280431/149‑1 (MQ=255)
                                                                                                                                                                                                                         atgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCgg                                                                                                                                                <  8:911669/141‑1 (MQ=255)
                                                                                                                                                                                                                         atgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCgg                                                                                                                                                >  7:911669/4‑144 (MQ=255)
                                                                                                                                                                                                                         atgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCa                                                                                                                                           >  6:1347813/4‑149 (MQ=255)
                                                                                                                                                                                                                         aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAttt                                                                                                                                                                                       >  8:103696/1‑105 (MQ=255)
                                                                                                                                                                                                                         aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAttt                                                                                                                                                                                       <  7:103696/105‑1 (MQ=255)
                                                                                                                                                                                                                             ttCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGt                                                                                                                                       <  7:64514/149‑1 (MQ=255)
                                                                                                                                                                                                                                   aaTATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa                                                                                                                                  <  7:763553/149‑1 (MQ=255)
                                                                                                                                                                                                                                    atattGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaaa                                                                                                                                 <  7:369826/145‑1 (MQ=255)
                                                                                                                                                                                                                                        tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGa                                                                                                          >  3:48793/1‑169 (MQ=255)
                                                                                                                                                                                                                                          cTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTc                                                                                                                            <  6:730634/149‑1 (MQ=255)
                                                                                                                                                                                                                                           tGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTcc                                                                                                                           >  8:525771/1‑149 (MQ=255)
                                                                                                                                                                                                                                            gAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCcg                                                                                                                          >  5:236829/1‑149 (MQ=255)
                                                                                                                                                                                                                                            gAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGAtttt                                                                                                      <  1:48793/169‑1 (MQ=255)
                                                                                                                                                                                                                                             aaGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                                                                                                                                                     >  2:166436/1‑41 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                        
AACAAGGGATCGCCGATCTGCGCGGTCTTTCCCCGCTTCAACGCGCCCGCACTATCATTGATAATTGTGCACATCCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGAAAG  >  NC_000913/3066081‑3066583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: