Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 6.8% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A. 6.8% 776.2 / 44.4 219intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (116/88);  new base . (7/8);  total (123/96)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 222 total aligned reads displayed.

GCTGCTGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA  >  NC_000913/4520298‑4520768
                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                             
gctgctGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAACGACGGAGACATGATCCGGCTTTACAGTATTACTGAGAATACCACCATCAACAATAATACAAGGTTCAAAACCCCCCCttt                                                                                                                                                                                                      >  4:106706/1‑275 (MQ=255)
                                                  gggAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATGCGTCACCTTGCAGACGGTTGCCGTCCCTCACTaa                                                                                                                                                    >  1:33240/1‑275 (MQ=255)
                                                      aaTGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGgaga                                                                                                                                                                                                                                                                               <  8:105147/149‑1 (MQ=255)
                                                         gCTCGTATTGACTCTGTTTACACGGAGATGTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGACACAt                                                                                                                                                                                                                                                                            >  8:541107/1‑149 (MQ=255)
                                                           tCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGc                                                                                                                                                                                                                                                                         >  6:172671/1‑149 (MQ=255)
                                                              tCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTcc                                                                                                                                                                                                                                                                      >  6:349585/1‑149 (MQ=255)
                                                                  aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCt                                                                                                                                                                                                                                                                  <  7:244468/149‑1 (MQ=255)
                                                                   aCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                                                                                                                                                                                                 <  8:700011/149‑1 (MQ=255)
                                                                    cccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATTCTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                                                                                                                                                                                                <  8:830363/149‑1 (MQ=255)
                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                                                                                                                                                                                               <  6:1186192/149‑1 (MQ=255)
                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                                                                                                                                                                                               <  7:237117/149‑1 (MQ=255)
                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                                                                                                                                                                                               >  5:905501/1‑149 (MQ=255)
                                                                          ttACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTa                                                                                                                                                                                                                                                          <  7:1311200/149‑1 (MQ=255)
                                                                             cccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTAtgg                                                                                                                                                                                                                                                       <  5:102846/149‑3 (MQ=255)
                                                                              ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTAc                                                                                                                                                                                                                                                      >  5:546462/1‑149 (MQ=255)
                                                                               cGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                                                                                                                                                                                                     <  5:457801/149‑1 (MQ=255)
                                                                                   gagTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                                                                                                                     >  4:243342/1‑209 (MQ=255)
                                                                                    agTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                                                                                                                                                                                                <  5:1056135/149‑1 (MQ=255)
                                                                                     gTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCgg                                                                                                                                                                                                                                                                    >  5:346321/1‑128 (MQ=255)
                                                                                       tGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa                                                                                                                                                                                 <  2:243342/209‑1 (MQ=255)
                                                                                        gATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                                                                                                                                                                                            <  8:443388/149‑1 (MQ=255)
                                                                                          tGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                                                                                                                                          >  6:1127457/1‑149 (MQ=255)
                                                                                          tGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                                                                                                                                          >  7:661446/1‑149 (MQ=255)
                                                                                           gCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                                                                                                                                                                                         <  6:999782/149‑1 (MQ=255)
                                                                                              gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                                                                                                                                                                                      >  6:21876/1‑149 (MQ=255)
                                                                                                ttGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                    <  8:1084085/149‑1 (MQ=255)
                                                                                                ttGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                    <  8:1223304/149‑1 (MQ=255)
                                                                                                ttGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                    <  7:1117823/149‑1 (MQ=255)
                                                                                                   ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                                                                                                                                                 >  5:99733/1‑149 (MQ=255)
                                                                                                    cAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt                                                                                                                                                                                                                                >  8:685170/1‑149 (MQ=255)
                                                                                                         gCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                                                                                                                                                                                            >  3:42754/1‑101 (MQ=255)
                                                                                                         gCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                                                                                                                                                                                           >  7:570179/1‑149 (MQ=255)
                                                                                                           aTGCTTTTATTAAAATATTCTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGTTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTCCTGAGAATACACCCATCAAGCATAATCGaa                                                                                                                                                                                                                         <  8:1146155/149‑1 (MQ=255)
                                                                                                               ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                                                                                                                                                     >  7:1195965/1‑149 (MQ=255)
                                                                                                                tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                                                                                                                                                                                                    <  6:1368001/149‑1 (MQ=255)
                                                                                                                   ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                                                                                                                                                                                           >  5:1214129/1‑123 (MQ=255)
                                                                                                                      cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                                                                                                                                                                                              <  7:1006812/149‑1 (MQ=255)
                                                                                                                        gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                                                                                                                                                                                            >  6:819151/1‑149 (MQ=255)
                                                                                                                            ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCt                                                                                                                                                                                                        >  7:752963/1‑149 (MQ=255)
                                                                                                                            ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCt                                                                                                                                                                                                        >  6:427685/1‑149 (MQ=255)
                                                                                                                               tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                                                                                                                                                                                     <  6:41005/149‑1 (MQ=255)
                                                                                                                                aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                                                                                                                                                                                                    <  5:410407/149‑1 (MQ=255)
                                                                                                                                   cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGGCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGTCTTACTGGCAAGAACCCGATTATAAAACTAACATGGCAAGCAGAATAAAAAt                                                                   >  2:340475/1‑275 (MQ=255)
                                                                                                                                    ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                    <  7:379652/113‑1 (MQ=255)
                                                                                                                                         cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                                                                                                                           >  5:1147493/1‑149 (MQ=255)
                                                                                                                                         cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                                                                                                                           >  5:1356735/1‑149 (MQ=255)
                                                                                                                                         cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                                                                                                                           <  7:948186/149‑1 (MQ=255)
                                                                                                                                          aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                                                                                                                                          >  8:544678/1‑149 (MQ=255)
                                                                                                                                          aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                                                                                                                                          >  8:601649/1‑149 (MQ=255)
                                                                                                                                           tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                                                                                                                                                                                         >  6:625249/1‑149 (MQ=255)
                                                                                                                                           tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                                                                                                                                                                                         >  6:1300308/1‑149 (MQ=255)
                                                                                                                                             aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                                                                                                                                                                                       <  8:1296315/149‑1 (MQ=255)
                                                                                                                                             aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                                                                                                                                                                                       >  7:1269790/1‑149 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                    <  6:1036220/103‑1 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                                                                                                                                                                                                    >  7:450239/1‑135 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                      >  5:569300/1‑149 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                      >  7:1140723/1‑149 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                      >  7:1437277/1‑149 (MQ=255)
                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGa                                                        >  2:211935/1‑275 (MQ=255)
                                                                                                                                               aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                                                                                                                     <  6:1435614/149‑1 (MQ=255)
                                                                                                                                                   aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTTCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACCGTGAACAACAGGTTGGCTCACTGGCAAGAACCAGATTATAAAACTAAGCTGGCGCGCAGAATAAAAATCAACCAACTTATGGa                                                    >  4:67009/1‑274 (MQ=255)
                                                                                                                                                        cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAAAAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAagag                                              >  4:240340/1‑275 (MQ=255)
                                                                                                                                                          tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa                                                                                                                                                                          >  8:960983/1‑149 (MQ=255)
                                                                                                                                                           aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg                                                                                                                                                                         >  6:929941/1‑149 (MQ=255)
                                                                                                                                                             cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                                                                                                                                          >  7:32574/1‑130 (MQ=255)
                                                                                                                                                               tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACgg                                                                                                                                                                     <  5:281636/149‑1 (MQ=255)
                                                                                                                                                                 tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                                                                                                                                                                                           <  8:880833/77‑1 (MQ=255)
                                                                                                                                                                   aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc                                                                                                                                                                 <  8:1437277/149‑1 (MQ=255)
                                                                                                                                                                    gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                >  6:1449969/1‑149 (MQ=255)
                                                                                                                                                                    gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                >  8:232309/1‑149 (MQ=255)
                                                                                                                                                                     cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                                                                                                                                                                                        >  7:1044863/1‑92 (MQ=255)
                                                                                                                                                                     cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                                                                                                                                                                                        <  8:1044863/92‑1 (MQ=255)
                                                                                                                                                                       aTCGCCCGGTGTTCACACATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAGACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTAACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCg                                <  2:67009/274‑1 (MQ=255)
                                                                                                                                                                       aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                                                                                                                                             <  8:18550/149‑1 (MQ=255)
                                                                                                                                                                       aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                                                                                                                                             >  8:72890/1‑149 (MQ=255)
                                                                                                                                                                        tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                                                                                                                                            <  6:334812/149‑1 (MQ=255)
                                                                                                                                                                          gCCGGCTGTTCAACAATACTGTAAAGCAGGAGACATGCTACGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGACCCTGTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcact                                                                                                                                                          >  8:1349077/1‑149 (MQ=255)
                                                                                                                                                                            ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcactca                                                                                                                                                        <  7:687415/149‑1 (MQ=255)
                                                                                                                                                                                     ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                                                                                                                                               >  7:352884/1‑149 (MQ=255)
                                                                                                                                                                                     ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                                                                                                                                               <  8:353501/149‑1 (MQ=255)
                                                                                                                                                                                      cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                                                                                                                              <  5:699468/149‑1 (MQ=255)
                                                                                                                                                                                      cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                                                                                                                              <  8:838290/149‑1 (MQ=255)
                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                             >  5:855970/1‑149 (MQ=255)
                                                                                                                                                                                        cATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                                                                                                                                            <  8:1195965/149‑1 (MQ=255)
                                                                                                                                                                                           aCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                >  5:727714/1‑126 (MQ=255)
                                                                                                                                                                                           aCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                <  6:727714/126‑1 (MQ=255)
                                                                                                                                                                                           aCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa                                                                                                                                         <  6:1355272/149‑1 (MQ=255)
                                                                                                                                                                                             tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                                                                                                                       >  7:922264/1‑149 (MQ=255)
                                                                                                                                                                                             tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                                                                                                                       >  6:1239664/1‑149 (MQ=255)
                                                                                                                                                                                             tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                                                                                                                       >  7:177178/1‑149 (MQ=255)
                                                                                                                                                                                               tAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGATTCg                                                                                                                                                                                    >  1:65483/1‑102 (MQ=255)
                                                                                                                                                                                               tAAAGCCGGAGCATGTCTCCGTCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                                                                                                                                                   <  5:1463471/119‑1 (MQ=39)
                                                                                                                                                                                                 aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa                                                                                                                                   >  6:923383/1‑149 (MQ=255)
                                                                                                                                                                                                  aGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa                                                                                                                                  >  7:808997/1‑149 (MQ=255)
                                                                                                                                                                                                   gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAATCATAATCGAAGGTTCAATATCGCCCCTTCTTGATCACCAGCAATTCATCACCGAGCAGACGGTTGCCGTCACTCACTAATTTATAGGAGAAAGAGaac                                                                                                                                 >  8:981235/1‑149 (MQ=255)
                                                                                                                                                                                                   gCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaac                                                                                                                                 >  7:848297/1‑149 (MQ=255)
                                                                                                                                                                                                     cGGAGACATGCTCCGGCTTTACAGTATTCCTGCGAATCCACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTg                                                         <  4:349/219‑1 (MQ=255)
                                                                                                                                                                                                     cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                                                                                                                                                                                               <  6:716537/69‑1 (MQ=255)
                                                                                                                                                                                                      ggAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaacaac                                                                                                                              <  6:100509/149‑1 (MQ=255)
                                                                                                                                                                                                         gaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAgg                                                                                                                           <  6:556623/149‑1 (MQ=255)
                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                             
GCTGCTGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA  >  NC_000913/4520298‑4520768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: