Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,016:1 +C 6.9% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0161.C 6.9% 744.5 / 52.3 175intergenic (+52/+32)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base . (84/79);  new base C (9/3);  total (93/82)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 175 total aligned reads displayed.

TGATGCAGATGTGTGTTGATACTTATTTTGCGCAGAGAAAACCGCTTCAGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCACGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGC  >  NC_000913/2389762‑2390279
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                        
agATGCAGATTTGTGTTGATACTTATTTTGCGCAGAGAAAACCGCTTCAGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTAc                                                                                                                                                                                                                                                     <  1:32078/274‑1 (MQ=255)
                                     aaaaCCTCTTTAGTCATTTACCCTTTTAANNAATTATGCCTGGATAATGTCCAGTGAAACGNCTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATNTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGca                                                                                                                                                                                                                <  3:41757/275‑1 (MQ=255)
                                                aGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                                                                                                                                            <  1:45543/220‑1 (MQ=255)
                                                            tGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCCTGTCGGCGGCTTTCTGACTTACaaa                                                                                                                                                                                                                                                  >  2:5070/1‑218 (MQ=255)
                                                                           ccTGGATAAGGGCCAGGGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                                                                                                                                          <  4:231385/275‑1 (MQ=255)
                                                                                   aTGTGCAGGGAAAAGAGTGGAATTCAGGAGCGGTAAGAGTTTAATAACGATGAATGGCGGGCAAAATTCAGGGAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCaa                                                                                                                                                                  <  4:184411/275‑1 (MQ=255)
                                                                                               aCGACTTCATTTACGGAACGCTAGTATTTTATTATCGATGAATGGCGGGCAAAATTAAGGCAGTTATTTTTAGAGGATTTTGGCGATAGTCGTACACAGTTCACCTAACGCTCTGCGTCTATCTACGATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                                                                                                                                      <  4:322039/275‑1 (MQ=255)
                                                                                                     gCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCCTACACCCAGCACCAGTACCAACACTGCAAAGATCAGca                                                                                                                                                 >  4:217515/1‑274 (MQ=255)
                                                                                                            aGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTc                                                                                                                                                                                                                                                                        >  6:1474909/1‑149 (MQ=255)
                                                                                                             ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcg                                                                                                                                                                                                                                                                       >  8:186676/1‑149 (MQ=255)
                                                                                                                 cGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGACCAACAGCCTAGCATGTCGGCGGCTTTCTGACTTACCAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGATAATCAGCGGTTGCTGCCATACACCCCGCACCCGTACCAACAGTACAGCGATCAGCCACACGATAccgc                                                                                                                                     >  2:33432/1‑274 (MQ=255)
                                                                                                                   cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGCGTATTTTGACGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTGTTAAGAAAAACCGCCGACATGCTcggcggtt                                                                                                                                                                                                                                                                 <  8:894955/149‑3 (MQ=255)
                                                                                                                       gATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTCAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                                                                                                                                            <  8:101604/149‑1 (MQ=255)
                                                                                                                            tAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACCGTCCCAAGATCAGCCCAACGATACCGCCCGCGCtctcct                                                                                                                         >  4:244234/1‑270 (MQ=255)
                                                                                                                                atCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                                                                                                                                   >  7:63073/1‑149 (MQ=255)
                                                                                                                                 tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                                                                                                                                         <  8:476148/142‑1 (MQ=255)
                                                                                                                                  cGATGAATGGCAGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGTCTTTCTGACTTACAAAg                                                                                                                                                                                                                                                 <  8:1220256/149‑1 (MQ=255)
                                                                                                                                   gATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                                                                                                                                                <  6:568052/149‑1 (MQ=255)
                                                                                                                                    aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTa                                                                                                                                                                                                                                               <  5:443068/149‑1 (MQ=255)
                                                                                                                                     tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                                                                                                                                              <  5:842743/149‑1 (MQ=255)
                                                                                                                                     tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCCACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                                                                                                                                              <  6:1416423/149‑1 (MQ=255)
                                                                                                                                     tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCAATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                                                                                                                                              <  6:801375/149‑1 (MQ=255)
                                                                                                                                              ggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTCGCGATAGTCGTACACAGTTCACTTAACGCTCTTCGTCTATCTACTATCCCTTCTTTTTCATAAAAACCGCCGAGCATGTCGGCTGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCTTTTCCAAACTCTCAATTCTAATCACCGGTTGTGCCTATACACCCAGCCCCAGTCCCAACAGTGCAGAGATCAGCACAACGATAACGCCCGCCCCTTCCTGCCCGTTTGATGTTGCac                                                                                                       >  4:113118/1‑274 (MQ=255)
                                                                                                                                                 aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                                                                                                                                   <  6:1204453/132‑1 (MQ=255)
                                                                                                                                                 aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                                                                                                                                   >  5:1204453/1‑132 (MQ=255)
                                                                                                                                                        aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                                                                                                                                              <  6:853484/130‑1 (MQ=255)
                                                                                                                                                        aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAt                                                                                                                                                                                                                           >  8:461139/1‑149 (MQ=255)
                                                                                                                                                         ggCAGTTAGGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTCTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTCACCGATTACATCAGCGGCAtt                                                                                                                                                                                                                          <  6:260253/149‑1 (MQ=255)
                                                                                                                                                                  gTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                                                                                                                                                 <  5:1429147/149‑1 (MQ=255)
                                                                                                                                                                   tttAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGca                                                                                                                                                                                                                >  6:20647/1‑149 (MQ=255)
                                                                                                                                                                        agTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaaa                                                                                                                                                                                                                    >  1:71014/1‑140 (MQ=255)
                                                                                                                                                                        agTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTg                                                                              >  2:315724/1‑274 (MQ=255)
                                                                                                                                                                            ttttGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGca                                                                                                                                                                                                                <  3:71014/140‑1 (MQ=255)
                                                                                                                                                                                     tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                                                                                                                                              <  7:7079/149‑1 (MQ=255)
                                                                                                                                                                                       gTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                                                                                                                                            <  5:1277872/149‑1 (MQ=255)
                                                                                                                                                                                        tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                                                                                                                                                      >  7:1351222/1‑138 (MQ=255)
                                                                                                                                                                                        tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                                                                                                                                                      <  8:1351222/138‑1 (MQ=255)
                                                                                                                                                                                        tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                                                                                                                                                  <  6:914887/142‑1 (MQ=255)
                                                                                                                                                                                        tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                                                                                                                                                  >  5:914887/1‑142 (MQ=255)
                                                                                                                                                                                        tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                                                                                                                                           >  7:481236/1‑149 (MQ=255)
                                                                                                                                                                                         cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                                                                                                                                          <  6:628419/149‑1 (MQ=255)
                                                                                                                                                                                         cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                                                                                                                                          <  6:555531/149‑1 (MQ=255)
                                                                                                                                                                                            acacAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCa                                                                                                                                                                                       <  8:1121417/149‑1 (MQ=255)
                                                                                                                                                                                            acacAGCTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCa                                                                                                                                                                                       >  7:272953/1‑149 (MQ=255)
                                                                                                                                                                                                 gttaccTTTACCCTCGGCTTTTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCCGGCATGTCGGCGGCTTTCTGACTTACAAAGTACAAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCGGCGGTGGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCACGCGCa                                                    <  4:258048/270‑1 (MQ=255)
                                                                                                                                                                                                 gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                                                                                                                                                  >  5:1081042/1‑149 (MQ=255)
                                                                                                                                                                                                  ttCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACNATGCTAANAAGNGGTTGTNGCCATa                                                                                                                                                                                     >  3:19229/1‑145 (MQ=255)
                                                                                                                                                                                                    cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                                                                                                                                               >  7:381611/1‑149 (MQ=255)
                                                                                                                                                                                                      cTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACAcc                                                                                                                                                                                 <  1:19229/145‑1 (MQ=255)
                                                                                                                                                                                                      cTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGc                                                                                                                                                                             >  6:702265/1‑149 (MQ=255)
                                                                                                                                                                                                       ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                                                                                                                                                      >  8:1319991/1‑123 (MQ=255)
                                                                                                                                                                                                       ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                                                                                                                                            >  7:1085226/1‑149 (MQ=255)
                                                                                                                                                                                                         aaCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                                                                                                                                          >  5:1020505/1‑149 (MQ=255)
                                                                                                                                                                                                          aCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCCTCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGt                                                                                                                                                                       >  2:367271/1‑151 (MQ=255)
                                                                                                                                                                                                          aCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGgttg                                                                                          >  2:143978/1‑228 (MQ=255)
                                                                                                                                                                                                            gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                                                                                                                                            <  8:671068/144‑1 (MQ=255)
                                                                                                                                                                                                              tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACa                                                                                                                                                                                                                                     <  7:487427/85‑1 (MQ=255)
                                                                                                                                                                                                              tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCATCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCa                                                                                                                                                                   <  4:367270/151‑1 (MQ=255)
                                                                                                                                                                                                                tGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                                                                                                                                              <  5:654769/90‑1 (MQ=255)
                                                                                                                                                                                                                tGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                                                                                                                                              >  6:654769/1‑90 (MQ=255)
                                                                                                                                                                                                                    tctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCAGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCaa                                                                                                                                                                  <  2:183885/146‑1 (MQ=255)
                                                                                                                                                                                                                     ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGt                                                                                                                                                              <  7:1172885/149‑1 (MQ=255)
                                                                                                                                                                                                                        tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                                                                                                                                           <  8:459118/149‑1 (MQ=255)
                                                                                                                                                                                                                        tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                                                                                                                                           >  5:993959/1‑149 (MQ=255)
                                                                                                                                                                                                                          tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGTGGTtgt                                                                                                                                                                                            >  7:476562/1‑114 (MQ=255)
                                                                                                                                                                                                                            ctaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtg                                                                                                                                                                                             <  2:243230/111‑1 (MQ=255)
                                                                                                                                                                                                                            ctaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAgaga                                                                                                                                                       <  6:468145/149‑1 (MQ=255)
                                                                                                                                                                                                                             taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                                                                                                                                      <  5:489069/149‑1 (MQ=255)
                                                                                                                                                                                                                               tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                                                                                                                                    <  7:911179/149‑1 (MQ=255)
                                                                                                                                                                                                                                cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                                                                                                                                                   <  5:1335276/149‑1 (MQ=255)
                                                                                                                                                                                                                                 ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTCATCAGCGGTTGTGGCCATACACCCAGCACCAGTATCAACAGTGCAGAGATCAGc                                                                                                                                                  <  8:1008760/149‑1 (MQ=255)
                                                                                                                                                                                                                                  cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCCTACAccc                                                                                                                                                                                >  4:157823/1‑118 (MQ=255)
                                                                                                                                                                                                                                  cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                                                                                                                                                 >  7:10539/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                                                                                                                                                 >  6:361040/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACACTGCAGAGATCAGca                                                                                                                                                 >  7:1224578/1‑149 (MQ=255)
                                                                                                                                                                                                                                   ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                                                                                                                                            <  7:141320/57‑1 (MQ=255)
                                                                                                                                                                                                                                   ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                                                                                                                                            >  8:141320/1‑57 (MQ=255)
                                                                                                                                                                                                                                   ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                                                                                                                                                >  5:1467016/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGTACCAGTACCAACAGTGCAGAGATCAGCACAAc                                                                                                                                             >  6:607636/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                                                                                                                                             >  5:732462/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTAGCCATACACCCAGCa                                                                                                                                                                            <  2:157823/118‑1 (MQ=255)
                                                                                                                                                                                                                                        tttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGa                                                                                                                                           >  7:461839/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCaa                                                                                                                                                                  >  7:765737/1‑122 (MQ=255)
                                                                                                                                                                                                                                            aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCACGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGcacc         <  2:194685/275‑1 (MQ=255)
                                                                                                                                                                                                                                             gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCa                                                                                                                                                                         >  6:1280633/1‑114 (MQ=255)
                                                                                                                                                                                                                                             gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                                                                                                                                      >  6:34300/1‑149 (MQ=255)
                                                                                                                                                                                                                                              gaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                                                                                                                                      >  6:1023111/2‑149 (MQ=255)
                                                                                                                                                                                                                                              gaaagCCGCCGACATGCTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc                                                                                                                                     >  6:34576/2‑149 (MQ=255)
                                                                                                                                                                                                                                               aaagccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                                                                                                                                           <  6:1196107/123‑1 (MQ=38)
                                                                                                                                                                                                                                               aaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                                                                                                                                           >  5:1196107/1‑127 (MQ=38)
                                                                                                                                                                                                                                               aaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc                                                                                                                                     >  8:798811/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                                                                                                                                             >  7:702937/1‑138 (MQ=255)
                                                                                                                                                                                                                                                 aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                                                                                                                                             <  8:702937/138‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccgccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCACGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGc  <  2:245280/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccgc                                                                                                                                 >  5:275520/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      ccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTg                                                                                                                             >  8:483404/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       cgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGt                                                                                                                            <  6:732462/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa                                                                                                                           <  7:172260/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa                                                                                                                           >  6:1480563/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         aGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTAc                                                                                                                          <  6:1108480/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                        
TGATGCAGATGTGTGTTGATACTTATTTTGCGCAGAGAAAACCGCTTCAGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCACGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGC  >  NC_000913/2389762‑2390279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: