Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,522 G→T 5.5% A26A (GCC→GCA yadK ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,5220GT 5.5% 714.5 / 23.1 201A26A (GCC→GCAyadKputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (85/105);  new base T (3/8);  total (88/113)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 203 total aligned reads displayed.

AGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGC  >  NC_000913/151263‑151792
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
    agcagcCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGTTTCCGTTTTTGCAGGAGACGTTTTTTACATTGGGAACAAGGTCAATATTCTGTCCATCAAGGGCTGGAGCGGCGATGCaaa                                                                                                                                                                                                                                                             >  4:26689/1‑275 (MQ=255)
         ccATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGt                                                                                                                                                                                                                                                        >  2:188962/1‑275 (MQ=255)
                 cctcTAACGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCACTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGac                                                                                                                                                                                                                                                  <  1:73437/270‑1 (MQ=255)
                                     aaTATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACGCTTTTCCACCTGGGTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGGCGGCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCGGTCCAGCAATGGATGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca                                                                                                                                                                                                                            <  2:4906/275‑1 (MQ=255)
                                                                 gTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatata                                                                                                                                                                                                <  2:20785/275‑1 (MQ=255)
                                                                                        tACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTg                                                                                                                                                                         <  4:188962/275‑1 (MQ=255)
                                                                                             tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTCCGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAg                                                                                                                                                                             >  4:220352/1‑266 (MQ=255)
                                                                                             tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCAAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAACCAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttagt                                                                                                                                                                    >  2:169090/1‑275 (MQ=255)
                                                                                                 aGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGac                                                                                                                                                                                                                                                  <  2:253090/193‑1 (MQ=255)
                                                                                                 aGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtata                                                                                                                                                                >  2:160889/1‑275 (MQ=255)
                                                                                                     aaCTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt                                                                                                                                                                                                                              >  4:150529/1‑209 (MQ=255)
                                                                                                         ctTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc                                                                                                                                                                                                                          <  2:150529/209‑1 (MQ=255)
                                                                                                                aCCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATgg                                                                                                                                                                                                                                                                               >  5:1108452/1‑149 (MQ=255)
                                                                                                                  cTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt                                                                                                                                                                                                                                                                             >  7:259297/1‑149 (MQ=255)
                                                                                                                       tGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa                                                                                                                                                                                                                                                                        <  5:991839/149‑1 (MQ=255)
                                                                                                                        gATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAg                                                                                                                                                                                                                                                                       >  8:1308526/1‑149 (MQ=255)
                                                                                                                           aaCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCg                                                                                                                                                                                                                                                                    >  6:1324412/1‑149 (MQ=255)
                                                                                                                           aaCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGGGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCg                                                                                                                                                                                                                                                                    >  8:1253577/1‑149 (MQ=255)
                                                                                                                              tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg                                                                                                                                                                                                                                                                 >  8:223046/1‑149 (MQ=255)
                                                                                                                                 cAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaa                                                                                                                                                                                                                                                              <  5:280314/149‑1 (MQ=255)
                                                                                                                                   ggAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaaa                                                                                                                                                                                                                                                            <  6:1393823/149‑1 (MQ=255)
                                                                                                                                     aGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa                                                                                                                                                                                                                                                          <  8:669719/149‑1 (MQ=255)
                                                                                                                                      gTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAg                                                                                                                                                                                                                                                         >  6:909747/1‑149 (MQ=255)
                                                                                                                                         aCATTACCTGAAAAATATCCCTCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTaa                                                                                                                                                                                                                                                      <  7:1195729/149‑1 (MQ=255)
                                                                                                                                          cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAAt                                                                                                                                                                                                                                                     <  7:74172/149‑1 (MQ=255)
                                                                                                                                          cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAAt                                                                                                                                                                                                                                                     <  6:520641/149‑1 (MQ=255)
                                                                                                                                          cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCCGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAAt                                                                                                                                                                                                                                                     <  7:664231/149‑1 (MQ=255)
                                                                                                                                           aTTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGc                                                                                                                                                                                                                                                                >  5:1386801/1‑137 (MQ=255)
                                                                                                                                           aTTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATg                                                                                                                                                                                                                                                    >  8:342179/1‑149 (MQ=255)
                                                                                                                                           aTTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc                                                                                                                                                                                                                          <  4:172191/175‑1 (MQ=255)
                                                                                                                                                 tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                                                                                                                                                                                                                     <  8:1452290/126‑1 (MQ=255)
                                                                                                                                                 tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaa                                                                                                                                                                                                                                              >  8:1184893/1‑149 (MQ=255)
                                                                                                                                                 tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaa                                                                                                                                                                                                                                              >  5:656169/1‑149 (MQ=255)
                                                                                                                                                  gAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaa                                                                                                                                                                                                                                             <  6:756006/149‑1 (MQ=255)
                                                                                                                                                  gAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGGCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaa                                                                                                                                                                                                                                             <  6:1269989/149‑1 (MQ=255)
                                                                                                                                                  gAAAAATATCCCACCCCAACAACGCCAAGATCAATATGACCCTGGTTACTTTTTCCGCTTTTGCAGCGGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGCCTGGTCAGGCGATGCAAAACAGTAATGacagaaa                                                                                                                                                                                                                                             >  6:1206970/1‑149 (MQ=255)
                                                                                                                                                       atatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacagaa                                                                                                                                                                                                                                        <  5:1075394/149‑1 (MQ=255)
                                                                                                                                                         atCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCTTGTCCAGCCATTGCTg                                                                                                                                                                                                                                                                            >  5:931650/1‑111 (MQ=255)
                                                                                                                                                            ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                                                                                                                                                   >  8:1444666/1‑149 (MQ=255)
                                                                                                                                                            ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                                                                                                                                                   >  6:277778/1‑149 (MQ=255)
                                                                                                                                                              aCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct                                                                                                                                                                                                                                 <  6:957319/149‑1 (MQ=255)
                                                                                                                                                              aCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct                                                                                                                                                                                                                                 >  6:839121/1‑149 (MQ=255)
                                                                                                                                                              aCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct                                                                                                                                                                                                                                 <  8:259297/149‑1 (MQ=255)
                                                                                                                                                               ccccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                                                                                                                                                                                                                <  5:141483/149‑1 (MQ=255)
                                                                                                                                                                    acgacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat                                                                                                                                                                                                                           <  5:909747/149‑1 (MQ=255)
                                                                                                                                                                      gacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatca                                                                                                                                                                                                                         >  6:617058/1‑149 (MQ=255)
                                                                                                                                                                         gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCt                                                                                                                                                                                                                      >  5:1273737/1‑149 (MQ=255)
                                                                                                                                                                            aaGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAAt                                                                                                                                                                                                                                                     >  7:976482/1‑115 (MQ=255)
                                                                                                                                                                            aaGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAc                                                                                                                                                                                                                   <  7:1074659/149‑1 (MQ=255)
                                                                                                                                                                                   atatTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt                                                                                                                                                                                                            <  8:762187/149‑1 (MQ=255)
                                                                                                                                                                                     atTACCCTGGTTACTGATTCCGCTTTTGGAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCGATGGCTGGACAGGCGATGCAAAACAGGAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTGGAGTgg                                                                                                                                                                                                          <  8:1040895/149‑1 (MQ=255)
                                                                                                                                                                                       tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                        <  5:243885/149‑1 (MQ=255)
                                                                                                                                                                                        aCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt                                                                                                                                                                                                       <  7:346375/149‑1 (MQ=255)
                                                                                                                                                                                         cccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTc                                                                        >  2:319686/1‑275 (MQ=255)
                                                                                                                                                                                            tGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCat                                                                                                                                                                                                   >  8:8352/1‑149 (MQ=255)
                                                                                                                                                                                            tGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCat                                                                                                                                                                                                   <  7:1444666/149‑1 (MQ=255)
                                                                                                                                                                                             ggTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCata                                                                                                                                                                                                  <  6:1462892/149‑1 (MQ=255)
                                                                                                                                                                                             ggTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGc                                                                                                                                              <  4:37631/201‑1 (MQ=255)
                                                                                                                                                                                              gTTACTGATTCCGCTTTTGCAGTTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCGCTTCATCAGCTTACGTTGAGTGGGATGCatat                                                                                                                                                                                                 <  6:1448751/149‑1 (MQ=255)
                                                                                                                                                                                              gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTNACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATTTGNGTGGTGCAGAAAACTTACCCGCAGTGACATTTTTTACGGTTTTTTNTNTTTCTACGACCCGGCgatcaccccttg                                                                    >  3:34918/1‑263 (MQ=255)
                                                                                                                                                                                               ttACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatata                                                                                                                                                                                                <  7:333884/149‑1 (MQ=255)
                                                                                                                                                                                                tACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatatat                                                                                                                                                                                               <  7:405871/149‑1 (MQ=255)
                                                                                                                                                                                                 aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa                                                                                                                                                                                                                                                                        <  5:565393/75‑1 (MQ=39)
                                                                                                                                                                                                 aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCAtatata                                                                                                                                                                                              >  7:1337990/1‑149 (MQ=255)
                                                                                                                                                                                                   tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGc                                                                                                                                                                                                                                                                <  7:464403/81‑1 (MQ=255)
                                                                                                                                                                                                   tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGt                                                                                                                                                                                            >  7:1320115/1‑149 (MQ=255)
                                                                                                                                                                                                   tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGt                                                                                                                                                                                            >  6:969124/1‑149 (MQ=255)
                                                                                                                                                                                                   tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGt                                                                                                                                                                                            >  5:373892/1‑149 (MQ=255)
                                                                                                                                                                                                      ttCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTcc                                                                                                                                                                                         <  5:617058/149‑1 (MQ=255)
                                                                                                                                                                                                            ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTcc                                                                                                                                                                                         >  6:929038/1‑143 (MQ=255)
                                                                                                                                                                                                                 cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                              >  6:1096505/1‑149 (MQ=255)
                                                                                                                                                                                                                 cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                              <  8:458621/149‑1 (MQ=255)
                                                                                                                                                                                                                     tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCt                                                                                                                                                                          <  5:1346701/149‑1 (MQ=255)
                                                                                                                                                                                                                     tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCt                                                                                                                                                                          <  7:775185/149‑1 (MQ=255)
                                                                                                                                                                                                                       cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTacagattc                                                                                                                                                                        >  7:1307848/1‑142 (MQ=255)
                                                                                                                                                                                                                       cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAAAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCGCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgt                                                                                                                                                                        >  5:795663/1‑149 (MQ=255)
                                                                                                                                                                                                                         gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtta                                                                                                                                                                      <  8:520426/149‑1 (MQ=255)
                                                                                                                                                                                                                         gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCACTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTTTAAGTCTgtta                                                                                                                                                                      <  5:1206970/149‑1 (MQ=255)
                                                                                                                                                                                                                          ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAg                                                                                                                                                                                                                                                         >  8:28985/1‑65 (MQ=255)
                                                                                                                                                                                                                          ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAg                                                                                                                                                                                                                                                         <  7:28985/65‑1 (MQ=255)
                                                                                                                                                                                                                          ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                       <  4:319686/275‑1 (MQ=255)
                                                                                                                                                                                                                              ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtat                                                                                                                                                                 <  7:999589/149‑1 (MQ=255)
                                                                                                                                                                                                                                aCATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAg                                                                                                                                                               <  6:393866/149‑1 (MQ=255)
                                                                                                                                                                                                                                   ttGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt                                                                                                                                                                                           >  7:880579/1‑118 (MQ=255)
                                                                                                                                                                                                                                     ggCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTAc                                                                                                                                                          <  8:885061/149‑1 (MQ=255)
                                                                                                                                                                                                                                        aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGCAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGCGATGAATATATAGTACCTGAATCGGTAAGTCCGTTAGTTAAAGGTTACTGt                                                                                                                                                       <  8:589035/149‑1 (MQ=255)
                                                                                                                                                                                                                                        aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTa                                                                                                                                                           >  5:726997/1‑145 (MQ=255)
                                                                                                                                                                                                                                        aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTa                                                                                                                                                           <  6:726997/145‑1 (MQ=255)
                                                                                                                                                                                                                                        aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGt                                                                                                                                                       <  7:1253577/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGc                                                                                                                                              >  7:537094/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  atatCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCt                                                                                                                                             <  7:138297/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   tatCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTg                                                                                                                                            <  6:1377348/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         gTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtt                                                                                                                                                                       >  2:370652/1‑116 (MQ=255)
                                                                                                                                                                                                                                                          tCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGa                                                                                                                                     <  6:578774/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgt                                                                                                                                                                        >  6:25032/1‑111 (MQ=255)
                                                                                                                                                                                                                                                             aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa                                                                                                                                  <  6:656169/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa                                                                                                                                  >  7:886257/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              gCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAAc                                                                                                                                 >  8:171174/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGAAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGc  >  2:268769/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                  tGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTAc                                                                                                                             <  7:448218/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
AGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGC  >  NC_000913/151263‑151792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: