Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,036 G→C 5.0% T104S (ACT→AGT)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0360GC 5.0% 557.7 / 17.2 162T104S (ACT→AGT) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base G (79/70);  new base C (6/2);  total (88/74)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01

Only 100 of 165 total aligned reads displayed.

ATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGAGTGTGTTGAGATCCTGGCGAATGGTAACTTCAGAAACTCCAGTGGCTTTTGCCAGATCGG  >  NC_000913/1343792‑1344285
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         
aTCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAt                                                                                                                                                                                                                             >  4:94299/1‑275 (MQ=255)
     tACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGCTACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGATTCCGGGCGAGTAGGGCATTGCTGCTGCGATTTTct                                                                                                                                                                                                                        >  1:46148/1‑274 (MQ=255)
          atcatATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                                                                                                                                                                                   >  2:113466/1‑275 (MQ=255)
               atCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACTGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCCCGCCCAGATGCCGGGCGa                                                                                                                                                                                                                                                  >  4:144150/1‑239 (MQ=255)
                                                         aCCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGACCGGGCTGTTCAAAGGGCCAACCATACTTTCGCTTTTTTTCGGACACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGGGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt                                                                                                                                                                    <  4:231886/275‑1 (MQ=255)
                                                                aaTGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCCGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCtt                                                                                                                                                             <  2:94299/275‑1 (MQ=255)
                                                                       tGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGACACCTCGGCGAGTTAACGCttgcgggta                                                                                                                                                      >  2:252800/1‑268 (MQ=255)
                                                                              aTGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCCGGTTCACGCTTCAGCGTATAGTTGc                                                                                                                                               >  2:39104/1‑275 (MQ=255)
                                                                                                     gtgtCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                                                                                                                                                                                      >  6:627672/1‑149 (MQ=255)
                                                                                                         cAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                                                                                                                                                                                  <  8:1141953/149‑1 (MQ=255)
                                                                                                         cAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                                                                                                                                                                                  <  5:148836/149‑1 (MQ=255)
                                                                                                          aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                                                                                                                                                                                 >  8:1016675/1‑149 (MQ=255)
                                                                                                          aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                                                                                                                                                                                 >  6:555191/1‑149 (MQ=255)
                                                                                                           aaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGGCATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc                                                                                                                                                                                                                                                >  6:850126/1‑148 (MQ=255)
                                                                                                            aGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGGCATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgca                                                                                                                                                                                                                                               <  5:850126/149‑3 (MQ=255)
                                                                                                             gggCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                                                                                                                                                                              <  8:555450/149‑1 (MQ=255)
                                                                                                               gCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                                                                                                                                                                            >  5:910327/1‑149 (MQ=255)
                                                                                                                     cATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                                                                                                                                                                      >  6:174694/1‑149 (MQ=255)
                                                                                                                      aTACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgct                                                                                                                                                                                                                                     >  5:1409396/1‑149 (MQ=255)
                                                                                                                        aCTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTa                                                                                                                                                                                                                                               <  5:1240425/137‑1 (MQ=255)
                                                                                                                        aCTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTa                                                                                                                                                                                                                                               >  6:1240425/1‑137 (MQ=255)
                                                                                                                          tttCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTTACATTTTTCTTCTGCGCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                                                                                                                                                                                 >  5:44540/1‑149 (MQ=255)
                                                                                                                               cTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGGCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                                                                                                                                                                            >  7:949588/1‑149 (MQ=255)
                                                                                                                               cTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                                                                                                                                                                            >  5:1101360/1‑149 (MQ=255)
                                                                                                                                tttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt                                                                                                                                                                                                                           >  7:466150/1‑149 (MQ=255)
                                                                                                                                tttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt                                                                                                                                                                                                                           >  7:846975/1‑149 (MQ=255)
                                                                                                                                  tttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                                                                                                                                                                         <  5:69062/149‑1 (MQ=255)
                                                                                                                                    tttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                                                                                                                                                                       <  7:142363/149‑1 (MQ=255)
                                                                                                                                        tGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                                                                                                                                                                       <  5:1333373/145‑1 (MQ=255)
                                                                                                                                         gATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                                                                                                                                                                                  >  5:1216142/1‑149 (MQ=255)
                                                                                                                                         gATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                                                                                                                                                                                  <  5:988511/149‑1 (MQ=255)
                                                                                                                                          aTACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGa                                                                                                                                                                                                                 >  7:665532/1‑149 (MQ=255)
                                                                                                                                          aTACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGa                                                                                                                                                                                                                 <  8:1230054/149‑1 (MQ=255)
                                                                                                                                           tACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAt                                                                                                                                                                                                                >  8:61361/1‑149 (MQ=255)
                                                                                                                                            acacGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATg                                                                                                                                                                                                               >  7:11824/1‑149 (MQ=255)
                                                                                                                                             cacGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                                                                                                                                                                              <  6:1290651/149‑1 (MQ=255)
                                                                                                                                              acGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGt                                                                                                                                                                                                             >  6:676506/1‑149 (MQ=255)
                                                                                                                                                gCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                                                                                                                                                                          >  2:381832/1‑134 (MQ=255)
                                                                                                                                                 ccACCGAGCAAAATAATTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                                                                                                                                                                          <  7:1160764/149‑1 (MQ=255)
                                                                                                                                                   aCCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGc                                                                                                                                                                                                        >  7:1005140/1‑149 (MQ=255)
                                                                                                                                                    ccGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                                                                                                                                                                      <  4:381831/134‑1 (MQ=255)
                                                                                                                                                    ccGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                                                                                                                                                                       >  6:372729/1‑149 (MQ=255)
                                                                                                                                                        gCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                                                                                                                                                                                   <  7:618838/149‑1 (MQ=255)
                                                                                                                                                         cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                                                                                                                                                                                  <  8:1290081/149‑1 (MQ=255)
                                                                                                                                                           aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                                                                                                                                                                                   <  8:653667/130‑1 (MQ=255)
                                                                                                                                                              tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                                                                                                                                                                             <  5:1356983/149‑1 (MQ=255)
                                                                                                                                                               aaCTCCACAATGCGCGTGTTTCAGCAAATGGGCGATGTAGCTGCTGACCGGGATGATAGTGACAGTTTTCGTCTGCTCGCCCAGATTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGGTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGa                                                              <  4:77493/275‑1 (MQ=255)
                                                                                                                                                                aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg                                                                                                                                                                                           <  7:304167/149‑1 (MQ=255)
                                                                                                                                                                aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg                                                                                                                                                                                           <  8:724260/149‑1 (MQ=255)
                                                                                                                                                                aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg                                                                                                                                                                                           <  8:77155/149‑1 (MQ=255)
                                                                                                                                                                 cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc                                                                                                                                                                                          <  5:735736/149‑1 (MQ=255)
                                                                                                                                                                  ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCg                                                                                                                                                                                         >  5:958673/1‑149 (MQ=255)
                                                                                                                                                                    cacaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCAGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                                                                                                                                                                                       <  7:1354922/149‑1 (MQ=255)
                                                                                                                                                                     acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcg                                                                                                                                                                                      >  6:1105806/1‑149 (MQ=255)
                                                                                                                                                                       aaGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                                                                                                                                                                                    >  8:34240/1‑149 (MQ=255)
                                                                                                                                                                         ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                                                                                                                                                                             >  4:364008/1‑90 (MQ=255)
                                                                                                                                                                            gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                                                                                                                                                                               <  5:806131/149‑1 (MQ=255)
                                                                                                                                                                             cgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAt                                                                                                                                                                                                                                         <  2:364009/90‑1 (MQ=255)
                                                                                                                                                                                  ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                                                                                                                                         <  5:1082696/149‑1 (MQ=255)
                                                                                                                                                                                   tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatc                                                                                                                                          >  2:323858/1‑179 (MQ=255)
                                                                                                                                                                                    cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                                                                                                                                                                       <  7:707899/149‑1 (MQ=255)
                                                                                                                                                                                     aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                                                                                                                                                                      >  6:138931/1‑149 (MQ=255)
                                                                                                                                                                                      gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                                                                                                                                                                                   <  6:156097/135‑1 (MQ=255)
                                                                                                                                                                                      gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                                                                                                                                                                                   >  5:156097/1‑135 (MQ=255)
                                                                                                                                                                                       cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATAc                                                                                                                                      <  4:323858/179‑1 (MQ=255)
                                                                                                                                                                                          aTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGAGTGTGTTGAGATCCTGGCgctgtctc                                    >  2:272602/1‑267 (MQ=255)
                                                                                                                                                                                              gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGAGTGTGTTg                                                      <  4:272602/252‑1 (MQ=255)
                                                                                                                                                                                                gATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCa                                                                                                                                                           >  5:709677/1‑149 (MQ=255)
                                                                                                                                                                                                     aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGta                                                                                                                                                      <  7:933279/149‑1 (MQ=255)
                                                                                                                                                                                                      gctgctGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGtat                                                                                                                                                     >  5:260640/1‑149 (MQ=255)
                                                                                                                                                                                                          ttggccGTGATGTTAGTAGTATTTTGCTTCTGCTCGCCGAAGGTCCGGGGGATAAGGGCATTACTGCTGCCATTTTGGATAAAGAGGGTTTCGCCCGGTTGAGCCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGATCATCATACGGGGTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGAGTGTGTTGAGATCCTGGCGAATGGTAACTTCAGAAACTCCAg                    <  4:306629/270‑1 (MQ=255)
                                                                                                                                                                                                            gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCAATATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTAAGTTTTTCGCGTGTGTTGAGATCCTGGCGCTGGAGAACTTCAGAAACTCCAGTgg                 >  4:35046/1‑275 (MQ=255)
                                                                                                                                                                                                             aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCt                                                                                                                                              >  7:86249/1‑149 (MQ=255)
                                                                                                                                                                                                                  gatgatAGTGACATTTTTCTTCTGCTCGCACAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAGAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatca                                                                                                                                         <  8:466150/149‑1 (MQ=255)
                                                                                                                                                                                                                         gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCAc                                                                                                                                                                 >  1:38027/1‑118 (MQ=255)
                                                                                                                                                                                                                           gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACgg                                                                                                                                    <  6:1452059/145‑1 (MQ=255)
                                                                                                                                                                                                                           gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACgg                                                                                                                                    >  5:1452059/1‑145 (MQ=255)
                                                                                                                                                                                                                            aCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTc                                                                                                                               >  6:713998/1‑149 (MQ=255)
                                                                                                                                                                                                                            aCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACg                                                                                                                           >  2:119136/1‑153 (MQ=255)
                                                                                                                                                                                                                             cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCTCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCg                                                                                                                              <  6:706488/149‑1 (MQ=255)
                                                                                                                                                                                                                              aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGa                                                                                                                             >  6:136472/1‑149 (MQ=255)
                                                                                                                                                                                                                              aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGa                                                                                                                             >  5:817057/1‑149 (MQ=255)
                                                                                                                                                                                                                                ttttCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACg                                                                                                                           >  7:801801/1‑149 (MQ=255)
                                                                                                                                                                                                                                ttttCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcat                                                                                                                       <  4:119136/153‑1 (MQ=255)
                                                                                                                                                                                                                                   tcttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtca                                                                                                                        <  7:1266656/149‑1 (MQ=255)
                                                                                                                                                                                                                                     ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                                                                                                                                                                   <  5:1279272/104‑1 (MQ=255)
                                                                                                                                                                                                                                     ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                                                                                                                                                                   >  6:1279272/1‑104 (MQ=255)
                                                                                                                                                                                                                                      cctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcatca                                                                                                                     >  8:860120/5‑149 (MQ=255)
                                                                                                                                                                                                                                       ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCAc                                                                                                                    >  6:653309/4‑149 (MQ=255)
                                                                                                                                                                                                                                        tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACgg                                                                                                                                    <  7:645941/130‑1 (MQ=255)
                                                                                                                                                                                                                                        tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACgg                                                                                                                                    >  8:645941/3‑132 (MQ=255)
                                                                                                                                                                                                                                        tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTCCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACt                                                                                                                   >  5:388402/1‑149 (MQ=255)
                                                                                                                                                                                                                                         gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                                                                                                                                                                               >  7:755456/1‑88 (MQ=255)
                                                                                                                                                                                                                                         gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                                                                                                                                                                               <  8:755456/88‑1 (MQ=255)
                                                                                                                                                                                                                                          cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTAt                                                                                                                 >  7:201209/1‑149 (MQ=255)
                                                                                                                                                                                                                                           tCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                                                                                                                                                                                    >  6:1338283/1‑81 (MQ=255)
                                                                                                                                                                                                                                             gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCaa                                                                                                              <  8:1005140/149‑1 (MQ=255)
                                                                                                                                                                                                                                             gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCaa                                                                                                              <  6:1216142/149‑1 (MQ=255)
                                                                                                                                                                                                                                               ccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGc                                                                                                            <  8:1039109/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 agagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGa                                                                                                          >  7:350583/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         
ATCGGTACGCATCATATCGCGACCGGTAAATCCAGTTTCAGGTTGCCAGCCATCAATACCAATAAATGCTTTGCTGAAATGCACCTGTTGGATGCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCGAAACAGCAAAGCCATGTGCACGGCGGAGGTAACTCAGTTTTTCGAGTGTGTTGAGATCCTGGCGAATGGTAACTTCAGAAACTCCAGTGGCTTTTGCCAGATCGG  >  NC_000913/1343792‑1344285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑