Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,229 G→T 5.4% L278M (CTG→ATG)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,2290GT 5.4% 654.2 / 19.9 186L278M (CTG→ATG) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base G (94/82);  new base T (2/8);  total (96/90)
Fisher's exact test for biased strand distribution p-value = 5.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 186 total aligned reads displayed.

GAACCACTTCCCAGACCCGCAGTCAGAAACAGCCCCATAAATACGGCGTAAAAGGCGATGAAATTACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTA  >  NC_000913/1542967‑1543495
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                           
gAACCACTTCCCAGACCCGCAGTCAGAAACAGCCCCATAAATACGGCGTAAAAGGCGATGAAATTACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATACGGGAACTGGGTTTTTGCCAGCATGGCAAAAcc                                                                                                                                                                                                                                                                >  2:271148/1‑275 (MQ=255)
      cTTCCCAGACCCGCAGTCAGAAACAGCCCCATAAATACGGCGTAAAAGGCGATGAAATTACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAACTAGCACCACCAACCGACCGCGCGATGGCACCGAAAAATGGGCCAAAGAACGCCAGGACCAGAATCTTCCCATCCGGGAACTGGGTTTTTGCCAGGATGGCAAAACCCGCaca                                                                                                                                                                                                                                                          >  4:58896/1‑273 (MQ=255)
                                                                                                agcagGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGAGAACTGGGTTTTTGCCAGCATGGCAAAACCCGAAGAAAAACCGATAAACGAACCGAAGGGGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGAAGGAGTTGTAAGACAGGGAGCTGGTAGGCact                                                                                                                                                                >  1:33124/1‑273 (MQ=255)
                                                                                                 gcagGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGGTGAAAGACCGGGGCCTGGTCGGCCAtt                                                                                                                                                               >  4:42399/1‑275 (MQ=255)
                                                                                                 gcagGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCCCAGCCGCCAGAGATGCCAGCGGTGTAAGACAGGGAGCGGGTCGGGAAtt                                                                                                                                                               >  4:170842/1‑275 (MQ=255)
                                                                                                 gcagGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCCCAGCAGCCAGAGCTGCAGGCGTTGGAAGAACGGGAGCTGGGCGCCAAt                                                                                                                                                                >  4:56043/1‑274 (MQ=255)
                                                                                                                 cGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTgg                                                                                                                                               <  2:42399/275‑1 (MQ=255)
                                                                                                                    cATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCa                                                                                                                                                                                                                                                                           >  5:15320/1‑149 (MQ=255)
                                                                                                                     aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTTGCCAGCa                                                                                                                                                                                                                                                                           >  8:571790/1‑149 (MQ=255)
                                                                                                                      tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATg                                                                                                                                                                                                                                                                         >  5:659338/1‑149 (MQ=255)
                                                                                                                      tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATg                                                                                                                                                                                                                                                                         <  6:1441762/149‑1 (MQ=255)
                                                                                                                        aaaaTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGc                                                                                                                                                                                                                                                                       >  8:1332514/1‑149 (MQ=255)
                                                                                                                          aaTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCATGCTGGCaa                                                                                                                                                                                                                                                                    >  7:784930/1‑149 (MQ=255)
                                                                                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCaaaa                                                                                                                                                                                                                                                                  <  6:618973/149‑1 (MQ=255)
                                                                                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCaaaa                                                                                                                                                                                                                                                                  >  6:1192196/1‑149 (MQ=255)
                                                                                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCaaaa                                                                                                                                                                                                                                                                  <  7:1031558/149‑1 (MQ=255)
                                                                                                                            tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTGCCATGCTGGCaaaaa                                                                                                                                                                                                                                                                 <  6:134372/149‑2 (MQ=255)
                                                                                                                             aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCATGCTGGCaaaaa                                                                                                                                                                                                                                                                 <  5:238393/149‑2 (MQ=255)
                                                                                                                             aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAACAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAAc                                                                                                                                                                                                                                                                 <  5:1268252/149‑1 (MQ=255)
                                                                                                                                gTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTGCCATGCTGGCAAAAaccca                                                                                                                                                                                                                                                             <  8:1098635/149‑3 (MQ=255)
                                                                                                                                 ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGc                                                                                                                                                                                                                                                             >  7:307952/1‑149 (MQ=255)
                                                                                                                                 ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTGCCATGCTGGCAAAAacccag                                                                                                                                                                                                                                                            <  5:136626/149‑4 (MQ=255)
                                                                                                                                 ttGATCAACGTCACCCGCACGCCACCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGc                                                                                                                                                                                                                                                             <  7:1314691/149‑1 (MQ=255)
                                                                                                                                  tGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc                                                                                                                               >  2:315276/1‑274 (MQ=255)
                                                                                                                                     tCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTGCCATGCTGGCAAAAaccca                                                                                                                                                                                                                                                             <  5:1331260/144‑3 (MQ=255)
                                                                                                                                       aaCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGaaaa                                                                                                                                                                                                                                                       >  5:486329/1‑149 (MQ=255)
                                                                                                                                          gTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAAcc                                                                                                                                                                                                                                                    >  6:923421/1‑149 (MQ=255)
                                                                                                                                          gTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGCCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCTGAAAAAcc                                                                                                                                                                                                                                                    >  6:1208479/1‑149 (MQ=255)
                                                                                                                                            ctcccGCAACCTGCCGAACGTATCGGATATAGCGCCACCAGCCGACCGCGCGAAGACACCGACAAATGGGCCAAAGAACGCCGGGCGCAGAATATTCACATCCGGCAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAAACGa                                                                                                                                                                                                                                                  <  6:948708/147‑1 (MQ=255)
                                                                                                                                            cACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGCCCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCAAGCATGGCAAAACCCGCAGAAAAACCgc                                                                                                                                                                                                                                                  <  6:689654/149‑2 (MQ=255)
                                                                                                                                             aCCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGAt                                                                                                                                                                                                                                                 >  7:167628/1‑149 (MQ=255)
                                                                                                                                                cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGa                                                                                                                                                                                                                                                  >  6:214885/1‑145 (MQ=255)
                                                                                                                                                cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGa                                                                                                                                                                                                                                                  <  5:214885/145‑1 (MQ=255)
                                                                                                                                                    cgccgcCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGaa                                                                                                                                                                                                                                          <  8:610515/149‑1 (MQ=255)
                                                                                                                                                     gccgccGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAAc                                                                                                                                                                                                                                         <  7:193140/149‑1 (MQ=255)
                                                                                                                                                           gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAgg                                                                                                                                                                                                                                   <  5:1192196/149‑1 (MQ=255)
                                                                                                                                                             aCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACAAACCGAAGGTg                                                                                                                                                                                                                                 <  7:962052/149‑1 (MQ=255)
                                                                                                                                                               tgaTCGGAAAAAGCACCCCCAACCGGCCGCGGGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAg                                                                                                 <  4:271148/273‑1 (MQ=255)
                                                                                                                                                               gtATCGGTAATAGCACCACCAACCGAACGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGACGCAGAATATACACATCCGGGAACTTGGTTTTTGCCAGAATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAATGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAGTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAg                                                                                                 <  2:52546/274‑1 (MQ=255)
                                                                                                                                                                  tCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAg                                                                                                                                                                                                                            >  5:784256/1‑149 (MQ=255)
                                                                                                                                                                   cGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAgg                                                                                                                                                                                                                           >  5:63430/1‑149 (MQ=255)
                                                                                                                                                                     gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                                                                                                                                         >  7:371218/1‑149 (MQ=255)
                                                                                                                                                                     gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                                                                                                                                         >  8:1165860/1‑149 (MQ=255)
                                                                                                                                                                     gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                                                                                                                                         >  8:739490/1‑149 (MQ=255)
                                                                                                                                                                           gCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGAt                                                                                                                                                                                                                                                 <  5:360499/119‑1 (MQ=255)
                                                                                                                                                                           gCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAg                                                                                                                                                                                                                   >  8:809994/1‑149 (MQ=255)
                                                                                                                                                                            caccacCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAgg                                                                                                                                                                                                                  >  6:1123757/1‑149 (MQ=255)
                                                                                                                                                                               caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                                                                                                                                               >  8:772355/1‑149 (MQ=255)
                                                                                                                                                                                accaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                                                                                                                                              <  6:486329/149‑1 (MQ=255)
                                                                                                                                                                                    accgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGc                                                                            >  4:237798/1‑275 (MQ=255)
                                                                                                                                                                                      cgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGcc                                                                                                                                                                                                        <  8:1307425/149‑1 (MQ=255)
                                                                                                                                                                                        accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCCGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGAGGCGATCGt                                                                                                             >  1:57109/1‑238 (MQ=255)
                                                                                                                                                                                        accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCag                                                                                                                                                                                                      <  6:763372/149‑1 (MQ=255)
                                                                                                                                                                                        accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCag                                                                                                                                                                                                      <  7:174268/149‑1 (MQ=255)
                                                                                                                                                                                        accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGata                                                                                                                                          <  2:16176/209‑1 (MQ=255)
                                                                                                                                                                                         ccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCaga                                                                                                                                                                                                     <  6:721663/149‑1 (MQ=255)
                                                                                                                                                                                           gcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                                                                                                                                                   <  6:437057/149‑1 (MQ=255)
                                                                                                                                                                                               gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCa                                                                                                                                                                                               <  5:212087/149‑1 (MQ=255)
                                                                                                                                                                                                    cACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGt                                                                                                                                                                                          <  6:265173/149‑1 (MQ=255)
                                                                                                                                                                                                     aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                                                                                                                                         >  8:1073518/1‑149 (MQ=255)
                                                                                                                                                                                                     aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                                                                                                                                         >  5:202359/1‑149 (MQ=255)
                                                                                                                                                                                                     aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                                                                                                                                         <  7:670137/149‑1 (MQ=255)
                                                                                                                                                                                                     aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                                                                                                                                         >  6:1271434/1‑149 (MQ=255)
                                                                                                                                                                                                      ccGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                                                                                                                                        >  6:656768/1‑149 (MQ=255)
                                                                                                                                                                                                       cGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCagag                                                                                                                                                                                                    >  5:860495/1‑136 (MQ=255)
                                                                                                                                                                                                        gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                                                                                                                                              >  6:1157276/1‑93 (MQ=255)
                                                                                                                                                                                                        gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                                                                                                                                      >  6:810483/1‑149 (MQ=255)
                                                                                                                                                                                                        gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                                                                                                                                      >  8:339388/1‑149 (MQ=255)
                                                                                                                                                                                                         aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                                                                                                                                     <  7:739490/149‑1 (MQ=255)
                                                                                                                                                                                                         aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                                                                                                                                     <  6:1367679/149‑1 (MQ=255)
                                                                                                                                                                                                         aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGAATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                                                                                                                                     <  8:1181305/149‑1 (MQ=255)
                                                                                                                                                                                                               gggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                                                                                                                                               <  8:159067/149‑1 (MQ=255)
                                                                                                                                                                                                                  ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                                                                                                                                            >  5:1363102/1‑149 (MQ=255)
                                                                                                                                                                                                                   cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                                                                                                                                           <  7:284776/149‑1 (MQ=255)
                                                                                                                                                                                                                   cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                                                                                                                                           >  7:145491/1‑149 (MQ=255)
                                                                                                                                                                                                                    aaaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                                                                                                                                          >  7:1415113/1‑149 (MQ=255)
                                                                                                                                                                                                                     aaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                                                                                                                                               <  6:782507/143‑1 (MQ=255)
                                                                                                                                                                                                                      aGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTgg                                                                                                                                                                        >  5:1381766/1‑149 (MQ=255)
                                                                                                                                                                                                                         aCGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAGCGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCg                                                                                                                                                                     >  5:1066545/1‑149 (MQ=255)
                                                                                                                                                                                                                         aCGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCg                                                                                                                                                                     >  8:1142828/1‑149 (MQ=255)
                                                                                                                                                                                                                               ggCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGAAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAtt                                                                                                                                                               <  7:423540/149‑1 (MQ=255)
                                                                                                                                                                                                                                 cgcAGAATATTGACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAATCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                                                                                                                                                 >  5:1207054/1‑145 (MQ=255)
                                                                                                                                                                                                                                 cgcAAAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                                                                                                                                                 <  6:1207054/145‑1 (MQ=255)
                                                                                                                                                                                                                                  gcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAg                                                                                                                                                            >  8:1047127/1‑149 (MQ=255)
                                                                                                                                                                                                                                    aGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                                                                                                                                          <  8:367544/149‑1 (MQ=255)
                                                                                                                                                                                                                                     gAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                                                                                                                                      <  2:65992/120‑1 (MQ=255)
                                                                                                                                                                                                                                     gAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcg                                                                                                                                                         >  6:66953/1‑149 (MQ=255)
                                                                                                                                                                                                                                     gAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                                                                                                                                          <  7:1141462/148‑1 (MQ=255)
                                                                                                                                                                                                                                      aaTATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcgc                                                                                                                                                        >  7:1055693/1‑149 (MQ=255)
                                                                                                                                                                                                                                        tatTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGt                                                                                                                                                      <  5:1456042/149‑1 (MQ=255)
                                                                                                                                                                                                                                              caTCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTg                                                                                                                                                <  5:576238/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATTCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACCTCACCGGAACGTCGGCCTGCGGTACGCCATTGACGccagggaagg              >  2:304885/1‑267 (MQ=255)
                                                                                                                                                                                                                                                        cTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcat                                                                                                                                      <  7:718067/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         tGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcatt                                                                                                                                     >  5:1146885/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         tGGGTTTTGGCCAGAATGGCAAAACCCGCAGAAAAACCGAGAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTAAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATAc       <  2:170842/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            gggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                                                                                                                                            <  7:151807/106‑1 (MQ=255)
                                                                                                                                                                                                                                                              tttttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc                                                                                                                                 <  8:145491/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              tttttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc                                                                                                                                 <  7:1142828/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               ttttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATcc                                                                                                                                <  8:785578/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                tttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc                                                                                                                               <  5:517924/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                           
GAACCACTTCCCAGACCCGCAGTCAGAAACAGCCCCATAAATACGGCGTAAAAGGCGATGAAATTACCGGAGCCTGTGCCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTA  >  NC_000913/1542967‑1543495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: