Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,558 T→A 5.4% intergenic (+24/‑172) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5580TA 5.4% 786.4 / 25.3 222intergenic (+24/‑172)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (101/107);  new base A (8/4);  total (110/112)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 234 total aligned reads displayed.

TGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTAC  >  NC_000913/164290‑164831
                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                 
tGAGAATCGCAGAGGTGTTTGGCCAGGCCCCCAATCCGACGACCCCCCAGGGGCCGGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                                                                                                                                                             <  4:64448/275‑1 (MQ=255)
   gaATGCAAGAGATGTTTGGGGGAGCCACCAATCCGACGATCGCCCCGGGGCGCGGGCCGCTGGTGGTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAgga                                                                                                                                                                                                                                                                          <  4:299950/275‑2 (MQ=255)
     tggCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGGGGGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                                                                                                                                         <  2:322612/272‑1 (MQ=255)
                                                  gggCGCGGGCAGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATGTGGGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                                                                                                           <  2:134555/275‑1 (MQ=255)
                                                                                 ccacTGCCCAAAGGCCATTACAAATCACACGAGATTTTAGCGACTTCTGGAAAGGAGCTGACCGGGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGGGca                                                                                                                                                                                             <  4:273031/271‑1 (MQ=255)
                                                                                                     cAAATCCNAAGAGAGTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                                                                                                                                              <  3:68203/205‑1 (MQ=255)
                                                                                                                        gCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCtgt                                                                                                                                                                                                                                                                                   >  5:456669/1‑149 (MQ=255)
                                                                                                                        gCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCtgt                                                                                                                                                                                                                                                                                   <  6:1279740/149‑1 (MQ=255)
                                                                                                                                tGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAgg                                                                                                                                                                                                                                                                           >  7:661565/1‑149 (MQ=255)
                                                                                                                                tGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAgg                                                                                                                                                                                                                                                                           <  5:1047522/149‑1 (MQ=255)
                                                                                                                                 ggAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                                                                                                                                                          <  7:631642/149‑1 (MQ=255)
                                                                                                                                  gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                                                                                                                                         >  8:1416233/1‑149 (MQ=255)
                                                                                                                                  gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                                                                                                                                         >  6:1124404/1‑149 (MQ=255)
                                                                                                                                  gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                                                                                                                                         >  5:551180/1‑149 (MQ=255)
                                                                                                                                   aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                                                                                                                                                        >  6:1198853/1‑149 (MQ=255)
                                                                                                                                     aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                                                                                                                                                      <  5:1378151/149‑1 (MQ=255)
                                                                                                                                      ggAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                                                                                                                                                     <  6:456669/149‑1 (MQ=255)
                                                                                                                                         gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCATTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAACGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTTGGAGCGGGCCGGGGTg                                                                                                                                                                                                                                                                  >  8:668794/1‑149 (MQ=255)
                                                                                                                                          cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                                                                                                                                                 <  8:1308472/149‑1 (MQ=255)
                                                                                                                                          cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                                                                                                                                                 <  8:685483/149‑1 (MQ=255)
                                                                                                                                             aCCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                                                                                                                                              >  7:1458038/1‑149 (MQ=255)
                                                                                                                                               cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTAGCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGGGTGAGGGCa                                                                                                                                                                                                                                                            <  8:1237891/149‑1 (MQ=255)
                                                                                                                                                ggtaGGTTCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGTACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAATGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                                                                                                              <  2:27874/271‑1 (MQ=255)
                                                                                                                                                gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATGCCAAACATGTCTGGCCGAACGACCCCGCAAATAATGCACCGACGCGAAGGACGAAAAAGTATGCGTAAGTTTGGTTTTGTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGAGCAt                                                                                                                                                                                                                                                           <  6:851842/149‑1 (MQ=255)
                                                                                                                                                gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                                                                                                                                           >  6:1010941/1‑149 (MQ=255)
                                                                                                                                                  gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                                                                                                                                         <  8:36829/149‑1 (MQ=255)
                                                                                                                                                        cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct                                                                                                                                                                                                                          >  3:53204/1‑174 (MQ=255)
                                                                                                                                                         aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca                                                                                                                                                                                                                                            >  2:96627/1‑155 (MQ=255)
                                                                                                                                                            aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                                                                                                                                      <  1:53204/174‑1 (MQ=255)
                                                                                                                                                             agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCtgt                                                                                                                                                                                                                                                                                   >  5:1315901/1‑112 (MQ=255)
                                                                                                                                                                gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcac                                                                                                                                                                                                                                           >  6:941687/1‑149 (MQ=255)
                                                                                                                                                                    aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc                                                                                                                                                                                                                                       <  7:771195/149‑1 (MQ=255)
                                                                                                                                                                      aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac                                                                                                                                                                                                                                     >  6:1394719/1‑149 (MQ=255)
                                                                                                                                                                      aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                                                                                                                                         <  7:1384288/145‑1 (MQ=255)
                                                                                                                                                                        ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc                                                                                                                                                                                                                                   >  7:1312242/1‑149 (MQ=255)
                                                                                                                                                                           gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGcc                                                                                                  <  2:218172/275‑1 (MQ=255)
                                                                                                                                                                            ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGagag                                                                                                                                                                                                                                                                            <  6:652839/104‑1 (MQ=255)
                                                                                                                                                                            ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGagag                                                                                                                                                                                                                                                                            >  5:652839/1‑104 (MQ=255)
                                                                                                                                                                            ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                                                                                                                                                               <  5:635795/149‑1 (MQ=255)
                                                                                                                                                                                 ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAGAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGagag                                                                                                                                                                                                                                                                            >  5:753717/1‑99 (MQ=255)
                                                                                                                                                                                 ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAGAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGagag                                                                                                                                                                                                                                                                            <  6:753717/99‑1 (MQ=255)
                                                                                                                                                                                  cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                                                                                                                                         >  6:785074/1‑149 (MQ=255)
                                                                                                                                                                                    aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAc                                                                                         <  4:225147/275‑1 (MQ=255)
                                                                                                                                                                                        tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCttga                                                                                                                                                                                                                   <  8:418040/149‑4 (MQ=255)
                                                                                                                                                                                        tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                                                                                   <  8:136659/149‑1 (MQ=255)
                                                                                                                                                                                         gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                                                                                                                  <  7:1086382/149‑1 (MQ=255)
                                                                                                                                                                                          tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                                                                                                                                                 <  5:817303/149‑1 (MQ=255)
                                                                                                                                                                                          tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                                                                                                                                                 <  5:372907/149‑1 (MQ=255)
                                                                                                                                                                                           cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                                                                                                                                                >  5:800632/1‑149 (MQ=255)
                                                                                                                                                                                             ggCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg                                                                                                                                                                                                                                                                                 <  4:215816/82‑1 (MQ=255)
                                                                                                                                                                                              gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga                                                                                                                                                                                                             >  6:341442/1‑149 (MQ=255)
                                                                                                                                                                                              gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga                                                                                                                                                                                                             <  7:1343030/149‑1 (MQ=255)
                                                                                                                                                                                               ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTAGTTTTCTCCCTCTCCCTGTGGGAGAGGGACGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                                                                                                            <  6:33039/149‑1 (MQ=255)
                                                                                                                                                                                                cGGGCGACCCGGCAAATACTGCACCGACGCGAGTGACGAACAAGTATTCGTAAGGTTGGTTTGCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGGGCGCACGTTCACCCTCACCCTATCCCTCTCCCTTGAGGGAGAgg                                                                                                                                                                                                           <  6:738315/149‑1 (MQ=37)
                                                                                                                                                                                                cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                                                                                                                           <  5:1198853/149‑1 (MQ=255)
                                                                                                                                                                                                cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                                                                                                                           <  6:545109/149‑1 (MQ=255)
                                                                                                                                                                                                cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg                                                                                                                                                                                                           <  7:683112/149‑1 (MQ=255)
                                                                                                                                                                                                 ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg                                                                                                                                                                                                          <  5:1390068/149‑1 (MQ=255)
                                                                                                                                                                                                 ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                                                                                                                          <  5:341442/149‑1 (MQ=255)
                                                                                                                                                                                                     gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc                                                                                                                                                                                                      <  7:763986/149‑1 (MQ=255)
                                                                                                                                                                                                         cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                                                                                                                                                     <  7:1215176/82‑1 (MQ=255)
                                                                                                                                                                                                         cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                                                                                                                                                     >  8:1215176/1‑82 (MQ=255)
                                                                                                                                                                                                             aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc                                                                                                                                                                                                                       <  8:1026918/124‑1 (MQ=255)
                                                                                                                                                                                                             aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc                                                                                                                                                                                              >  5:150626/1‑149 (MQ=255)
                                                                                                                                                                                                              aaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                                                                                                                             >  5:1336319/1‑149 (MQ=255)
                                                                                                                                                                                                               aTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcac                                                                                                                                                                                            >  8:1338001/1‑149 (MQ=255)
                                                                                                                                                                                                                     cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc                                                                                                                                                                                                                                    <  5:696596/103‑1 (MQ=255)
                                                                                                                                                                                                                     cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGCGAGAGGGGACCTATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGCACAGACAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGTGAATGACCGCGAGCCAATTCGCCGTAAATGGACACCGAcg                                                        >  2:249503/1‑275 (MQ=255)
                                                                                                                                                                                                                        cgacgcggcgGACGAAAAAGTAGCCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGTGCCGGACCGAGGGTATCGGCGCGCACGTTGACCCTCATCTTAACCCTCACCCTCAAGGGAGAGGGGACCGAGCGAGTACAAATTCTga                                                                                                                                                                                   <  8:1403853/149‑1 (MQ=255)
                                                                                                                                                                                                                          acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                                                                                                                                                                                 <  6:837431/149‑1 (MQ=255)
                                                                                                                                                                                                                             gaagggagtaaaaaGGTTGCGTAAGGTTGGGTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGt                                                <  4:167550/266‑1 (MQ=255)
                                                                                                                                                                                                                              gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata                                                                                                                                                                             >  8:295865/1‑149 (MQ=255)
                                                                                                                                                                                                                                   acgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt                                                                                                                                                                                      <  8:450564/135‑1 (MQ=255)
                                                                                                                                                                                                                                     gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg                                                                                                                                                                                                          <  6:1348859/113‑1 (MQ=255)
                                                                                                                                                                                                                                     gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCGAGGGTGAGGGGACCGACCGAGGACACATCATGAGAGATAtcttctt                                                                                                                                                                      >  5:124268/1‑149 (MQ=37)
                                                                                                                                                                                                                                      aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                                                                                                                                     >  8:652304/1‑149 (MQ=255)
                                                                                                                                                                                                                                      aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                                                                                                                                     >  5:708763/1‑149 (MQ=255)
                                                                                                                                                                                                                                           gTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                                                                                                                >  5:94848/1‑149 (MQ=255)
                                                                                                                                                                                                                                            tATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                                                                                                                               <  5:1140502/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                                                                                                                                      >  7:1266763/1‑89 (MQ=255)
                                                                                                                                                                                                                                                 gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                                                                                                                                          >  6:1468610/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  tAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa                                                                                                                                                         <  8:1458038/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg                                                                                                                                                                                                     <  7:536976/102‑6 (MQ=25)
                                                                                                                                                                                                                                                     ggttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                                                                                                                      <  7:433935/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                                                                                                                                                  >  7:1410192/1‑104 (MQ=255)
                                                                                                                                                                                                                                                       ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                                                                                                                                                >  6:680272/1‑41 (MQ=255)
                                                                                                                                                                                                                                                         ggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                                                                                                                                  <  5:516525/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                                                                                                                                                >  8:172467/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                gccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt                                                                                                                                           >  8:539148/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                gccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt                                                                                                                                           >  5:1122556/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                 ccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                                                                                                             >  6:707583/1‑130 (MQ=255)
                                                                                                                                                                                                                                                                 ccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                                                                                                                                          >  7:245891/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  cctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                                                                                                                         >  7:734202/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  cctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                                                                                                                         >  7:604714/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   ctctccctGTGGGAGAGGGACGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTAGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg                                                                                                                                        >  6:58220/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt                                                                                                                                     >  7:285920/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           gtgGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCGCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGGCGTGTAACAGAGGAACAGAAAATCGGGGTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTac  <  1:12716/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                                                                                                                                   >  6:490099/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                            tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                                                                                                                                   <  5:490099/97‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGCTACGAAGATGACGATGATTa   >  2:60436/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                 
TGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTAC  >  NC_000913/164290‑164831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑