Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,952,587 T→A 5.0% W108R (TGG→AGG)  yecN → MAPEG family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,952,5870TA 5.0% 625.1 / 13.7 176W108R (TGG→AGG) yecNMAPEG family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (82/85);  new base A (4/5);  total (86/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 177 total aligned reads displayed.

CGCGTTGCCTATGGCGACGGCGGTTTTAGCGAACTGCAAAGCGCTATTCGCATTCATGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAAT  >  NC_000913/1952350‑1952824
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
cgcgTTGGCTATGGGGAATGCGGGTTTTGCGCACTGGAATGCGGTATTCGCATGCATTGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGCGGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCGGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGGGCTGGCGAATCttt                                                                                                                                                                                                           <  4:177635/274‑1 (MQ=255)
                                              ttCGCATTCATGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTTGCGACGTTCCGGCCTGAGCGCCACCTGGTCTGCGCTGTTGCTGATGGTTCTGGCGAATCTTTGGTTTATGCCCTGGGCGTTGGTTTTTTCCCTGCGTTAgccccccatc                                                                                                                                                            >  4:172084/1‑267 (MQ=255)
                                                                   tGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCATGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                                                       >  2:235216/1‑275 (MQ=255)
                                                                           atTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCCTATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGGGTTGGTTTTCTCCCTGCGTTAGCGCCAAATACGCCACTTTCTTTTTCCCGGCttttttc                                                                                                                                >  4:294605/1‑272 (MQ=255)
                                                                                           tgCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTgg                                                                                                                                                                                                                                             <  5:601928/149‑1 (MQ=255)
                                                                                           tgCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGc                                                                                                                                                                                                  >  2:259205/1‑192 (MQ=255)
                                                                                              tGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGgtgt                                                                                                                                                                                                                                          <  7:1301334/149‑1 (MQ=255)
                                                                                               gATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGtgtg                                                                                                                                                                                                                                         >  5:118029/1‑149 (MQ=255)
                                                                                               gATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTg                                                                                                                                                                                              <  4:259205/192‑1 (MQ=255)
                                                                                                aTGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTgc                                                                                                                                                                                                                                        <  5:1437680/149‑1 (MQ=255)
                                                                                                  gCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCACACCAGgtggcgc                                                                                                                                                                                                                                       <  8:1226513/149‑5 (MQ=255)
                                                                                                   cTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCt                                                                                                                                                                                                                                     >  8:506800/1‑149 (MQ=255)
                                                                                                    tGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTg                                                                                                                                                                                                                                    <  6:491690/149‑1 (MQ=255)
                                                                                                      tttATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGtt                                                                                                                                                                                                                                  >  7:1120512/1‑149 (MQ=255)
                                                                                                      tttATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGtt                                                                                                                                                                                                                                  <  7:320110/149‑1 (MQ=255)
                                                                                                        tATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCACACCAGGTGGCGCtcatgc                                                                                                                                                                                                                                <  8:233678/149‑6 (MQ=255)
                                                                                                          tGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGCTGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTTCGCTGTTGCTTATGGTGCTGGCGAATCTTTGGTATATGCCCTGGTAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                                                 <  2:260183/274‑1 (MQ=255)
                                                                                                           ggAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCGTTATTACGGTTTTCAGCACCGTCTGTTCCGCTGACGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGa                                                                                                                                                                                                                             >  6:1191943/1‑149 (MQ=255)
                                                                                                            gAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCACACCAGgtggcgc                                                                                                                                                                                                                                       >  8:1443175/1‑135 (MQ=255)
                                                                                                             aaaTGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATg                                                                                                                                                                                                                           <  6:213514/149‑1 (MQ=255)
                                                                                                                    tgGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTgg                                                                                                                                                                                                                    <  7:1389244/149‑1 (MQ=255)
                                                                                                                        gcAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGaa                                                                                                                                                                                                                >  8:479099/1‑149 (MQ=255)
                                                                                                                          aGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATc                                                                                                                                                                                                              >  6:239264/1‑149 (MQ=255)
                                                                                                                             aaCCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCttt                                                                                                                                                                                                           <  6:1296146/149‑1 (MQ=255)
                                                                                                                             aaCCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCttt                                                                                                                                                                                                           <  5:5902/149‑1 (MQ=255)
                                                                                                                              aCCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTg                                                                                                                                                                                                          <  6:904773/149‑1 (MQ=255)
                                                                                                                              aCCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTg                                                                                                                                                                                                          >  7:571916/1‑149 (MQ=255)
                                                                                                                               ccTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTgg                                                                                                                                                                                                         <  7:1152921/149‑1 (MQ=255)
                                                                                                                                      ggTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGc                                                                                                                                                                                                  <  5:770895/149‑1 (MQ=255)
                                                                                                                                         gCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGa                                                                                                                                                                                                                             >  6:1415408/1‑119 (MQ=255)
                                                                                                                                         gCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCt                                                                                                                                                                                               >  7:1084142/1‑149 (MQ=255)
                                                                                                                                          cATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTg                                                                                                                                                                                              <  7:180819/149‑1 (MQ=255)
                                                                                                                                           atatTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGt                                                                                                                                                                                                                         >  5:260426/1‑121 (MQ=255)
                                                                                                                                             atTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGa                                                                                                                                                                                           <  8:1138474/149‑1 (MQ=255)
                                                                                                                                             atTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGa                                                                                                                                                                                           <  5:974722/149‑1 (MQ=255)
                                                                                                                                              tttGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAg                                                                                                                                                                                          >  5:895194/1‑149 (MQ=255)
                                                                                                                                                 gCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTg                                                                                                                                                                                       <  5:1014971/149‑1 (MQ=255)
                                                                                                                                                  cGGCATCGTTTTGCTTGCTGGTCGTCTGATGCTTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGAATGGCCCTTTGATGAACgg                                                        >  2:341121/1‑274 (MQ=255)
                                                                                                                                                   ggCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCACACCAGgtggcgc                                                                                                                                                                                                                                       >  6:531125/1‑96 (MQ=255)
                                                                                                                                                    gCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGtt                                                                                                                                                                                    <  6:1002114/149‑1 (MQ=255)
                                                                                                                                                       tCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTgc                                                                                                                                                                                                                                        >  6:614935/1‑94 (MQ=255)
                                                                                                                                                        cGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTtct                                                                                                                                                                                <  7:32755/149‑1 (MQ=255)
                                                                                                                                                          ttttGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTcc                                                                                                                                                                              <  7:224184/149‑1 (MQ=255)
                                                                                                                                                              gcttgctGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGc                                                                                                                                                                          <  7:566795/149‑1 (MQ=255)
                                                                                                                                                                 gtgtGGTCTTCTGATGCATTATTCCGTTTTTCAGCACCGGTTGTTCCGCTGGCGCCGTTCCGGCATGAGGGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTTCGTTAGCGCACAATTCGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc                                         <  4:202794/272‑1 (MQ=255)
                                                                                                                                                                    tGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAgc                                                                                                                                                                    >  5:1258094/1‑149 (MQ=255)
                                                                                                                                                                    tGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAgc                                                                                                                                                                    >  5:1455703/1‑149 (MQ=255)
                                                                                                                                                                    tGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAgc                                                                                                                                                                    >  5:334325/1‑149 (MQ=255)
                                                                                                                                                                      gtcgtcTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAgcgc                                                                                                                                                                  >  8:461760/1‑149 (MQ=255)
                                                                                                                                                                      gtcgtcTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAgcgc                                                                                                                                                                  >  5:395756/1‑149 (MQ=255)
                                                                                                                                                                       tcgtcTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                                                 <  6:334325/149‑1 (MQ=255)
                                                                                                                                                                               tGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCTTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                                                         <  7:66526/149‑1 (MQ=255)
                                                                                                                                                                               tGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCAGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGGCCTGGGAGCTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                                                         >  8:1191181/1‑149 (MQ=255)
                                                                                                                                                                                gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGcc                                                                                                                                                        >  6:29337/1‑149 (MQ=255)
                                                                                                                                                                                 cATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCa                                                                                                                                                       <  5:29337/149‑1 (MQ=255)
                                                                                                                                                                                  attattACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTg                                                                                                                                                                                                          >  8:337115/1‑97 (MQ=255)
                                                                                                                                                                                   ttattaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAct                                                                                                                                                     >  5:1372842/1‑149 (MQ=255)
                                                                                                                                                                                   ttattaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAct                                                                                                                                                     >  7:883644/1‑149 (MQ=255)
                                                                                                                                                                                    tattaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGCCCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGtt                      >  4:52245/1‑275 (MQ=255)
                                                                                                                                                                                     atgaCGGTTTTCGCTACGGTCTGGTCCGCTGGCGACGGTCCGGCATGAGGGCAACCTGGTTGGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTATTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGtt                      <  4:121665/271‑1 (MQ=255)
                                                                                                                                                                                       taCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActttct                                                                                                                                                 >  7:125781/1‑149 (MQ=255)
                                                                                                                                                                                          ggTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGt                                                                                                                                                                                     <  5:494911/110‑1 (MQ=255)
                                                                                                                                                                                           gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCttttt                                                                                                                                             <  8:835751/149‑1 (MQ=255)
                                                                                                                                                                                           gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTAcc               >  2:167807/1‑275 (MQ=255)
                                                                                                                                                                                            ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTc                                                                                                                                            <  5:406727/149‑1 (MQ=255)
                                                                                                                                                                                            ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTc                                                                                                                                            >  7:1385066/1‑149 (MQ=255)
                                                                                                                                                                                            ttttCATCACCGACTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTc                                                                                                                                            >  8:642617/1‑149 (MQ=255)
                                                                                                                                                                                              ttctacgccGTCTGTTCCGCTGGCGACGTTCCGGCATGGGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTTGTTTTCGCCCTGCTTTAGCGCACAATACGCCACTTTCTTTGTCCCGGATTTTTACGTTATGTCTAACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTACCGAATATGATCCAGCGTTCCGTACCCgg            <  1:63273/268‑1 (MQ=255)
                                                                                                                                                                                                 atcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                                                       <  6:395756/149‑1 (MQ=255)
                                                                                                                                                                                                 atcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                                                       >  5:359941/1‑149 (MQ=255)
                                                                                                                                                                                                 atcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                                                       <  6:485185/149‑1 (MQ=255)
                                                                                                                                                                                                  tcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAt                                                                                                                                      >  8:1338250/1‑149 (MQ=255)
                                                                                                                                                                                                  tcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAt                                                                                                                                      >  6:336089/1‑149 (MQ=255)
                                                                                                                                                                                                   caCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAtt                                                                                                                                     >  6:740763/1‑149 (MQ=255)
                                                                                                                                                                                                    aCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAttt                                                                                                                                    <  8:1206648/149‑1 (MQ=255)
                                                                                                                                                                                                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                                                >  7:576979/1‑149 (MQ=255)
                                                                                                                                                                                                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                                                <  5:445956/149‑1 (MQ=255)
                                                                                                                                                                                                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                                                <  5:239264/149‑1 (MQ=255)
                                                                                                                                                                                                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACATTTGATGCACGGGTAGCTGAAGTCTTACCGGGTATATTCCATCGTTCCGTACCCCGCTATTcacat  >  1:36049/1‑271 (MQ=255)
                                                                                                                                                                                                            ttCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTa                                                                                                                            >  8:459823/1‑149 (MQ=255)
                                                                                                                                                                                                             tCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTAt                                                                                                                           <  8:1084142/149‑1 (MQ=255)
                                                                                                                                                                                                              ccGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATg                                                                                                                          <  8:571916/149‑1 (MQ=255)
                                                                                                                                                                                                                      gACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGGGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATg                                                                                                                          <  6:813979/141‑1 (MQ=255)
                                                                                                                                                                                                                      gACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATg                                                                                                                          >  5:813979/1‑141 (MQ=255)
                                                                                                                                                                                                                         gTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTACGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGa                                                                                                               >  5:1054667/1‑149 (MQ=255)
                                                                                                                                                                                                                          ttCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                                              >  5:65091/1‑149 (MQ=255)
                                                                                                                                                                                                                             cGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACg                                                                                                           >  8:553775/1‑149 (MQ=255)
                                                                                                                                                                                                                              ggCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGc                                                                                                          >  6:365662/1‑149 (MQ=255)
                                                                                                                                                                                                                              ggCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGc                                                                                                          <  7:479099/149‑1 (MQ=255)
                                                                                                                                                                                                                               gCATGAGCGCCACCTGGTGTGCACTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTTTGTCTCACCGCGACACGCt                                                                                                         >  6:1127546/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTAtt                                                                                                      >  8:1028214/1‑149 (MQ=255)
                                                                                                                                                                                                                                    agcgcacACCAGGTGGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTAtttt                                                                                                    >  8:1362062/7‑149 (MQ=255)
                                                                                                                                                                                                                                      cgcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCt                                                                                                  <  6:271019/149‑1 (MQ=255)
                                                                                                                                                                                                                                      cgcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCt                                                                                                  <  6:468246/149‑1 (MQ=255)
                                                                                                                                                                                                                                       gcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                                                 <  8:1055447/149‑1 (MQ=255)
                                                                                                                                                                                                                                          cACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcc                                                                                               <  8:907166/149‑1 (MQ=255)
                                                                                                                                                                                                                                             cTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                                                <  5:760024/113‑1 (MQ=255)
                                                                                                                                                                                                                                             cTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                                                >  6:760024/1‑113 (MQ=255)
                                                                                                                                                                                                                                             cTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCt                                                                                            <  7:461760/149‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                                                           >  8:1107632/1‑149 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
CGCGTTGCCTATGGCGACGGCGGTTTTAGCGAACTGCAAAGCGCTATTCGCATTCATGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAAT  >  NC_000913/1952350‑1952824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: