Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 167,917 A→C 5.9% Q145P (CAG→CCG) ‡ fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913167,9170AC 5.9% 758.6 / 30.5 215Q145P (CAG→CCG) ‡fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (110/91);  new base C (7/6);  total (118/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 218 total aligned reads displayed.

GGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGGATGATGACGGTGTTTACTCTTATCGCCTGACCGGTCTTGCGCGT  >  NC_000913/167647‑168179
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                      
                               gTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAATAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGt                                                                                                                                                                                                                                     <  4:78085/275‑1 (MQ=255)
                                                                               gTCTTTTTCTGTTGTGCCCGCCGGAGGGGNAGGGCGGCATCAGCCGAAGTGGGTCNAAGAAGCGCTTAGCTACCCGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGGNGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCttt                                                                                                                                                                                     <  3:59993/275‑1 (MQ=255)
                                                                                         gttgtGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTTTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAg                                                                                                                                                                           <  2:66267/275‑1 (MQ=255)
                                                                                          ttgtGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTTAATGGCCTGAAGTTGCCGGGCAACTTCCATAACGATCCGGTCATTGACCCGTATACGTTGGATCCCGGCTAAATTATGCGGGGCCCGGTTTCCCTCCTTTACGGATAAAga                                                                                                                                                                          >  4:186946/1‑274 (MQ=255)
                                                                                                      aaGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggcggc                                                                                                                                                              <  2:194203/275‑1 (MQ=255)
                                                                                                                               gTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAg                                                                                                                                                                                                                                                                       >  7:608400/1‑145 (MQ=255)
                                                                                                                               gTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAg                                                                                                                                                                                                                                                                       <  8:608400/145‑1 (MQ=255)
                                                                                                                               gTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCa                                                                                                                                                                                                                                                                   >  8:257206/1‑149 (MQ=255)
                                                                                                                                  ggTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACt                                                                                                                                                                                                                                                                <  5:884441/149‑1 (MQ=255)
                                                                                                                                  ggTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACt                                                                                                                                                                                                                                                                >  8:778041/1‑149 (MQ=255)
                                                                                                                                    tAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTc                                                                                                                                                                                                                                                              >  5:919929/1‑149 (MQ=255)
                                                                                                                                    tAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTc                                                                                                                                                                                                                                                              >  6:438806/1‑149 (MQ=255)
                                                                                                                                     aaaaGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCGGGGCAACTTCt                                                                                                                                                                                                                                                             >  7:946923/1‑149 (MQ=255)
                                                                                                                                     aaaaGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCt                                                                                                                                                                                                                                                             >  5:775820/1‑149 (MQ=255)
                                                                                                                                      aaaGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCCCTGCAACTTcag                                                                                                                                                                                                                                                            <  6:642879/149‑3 (MQ=255)
                                                                                                                                              gcTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATaa                                                                                                                                                                                                                                                         >  7:1168593/1‑144 (MQ=255)
                                                                                                                                              gcTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATaa                                                                                                                                                                                                                                                         <  8:1168593/144‑1 (MQ=255)
                                                                                                                                                ttAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCg                                                                                                                                                                                                                                                  <  5:1287738/149‑1 (MQ=255)
                                                                                                                                                ttAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCg                                                                                                                                                                                                                                                  >  7:714483/1‑149 (MQ=255)
                                                                                                                                                ttAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCg                                                                                                                                                                                                                                                  <  8:644616/149‑1 (MQ=255)
                                                                                                                                                    cTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGAGCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCa                                                                                                                                                                                                                                              >  6:1265223/1‑149 (MQ=255)
                                                                                                                                                     tACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCAt                                                                                                                                                                                                                                             <  6:1091646/149‑1 (MQ=255)
                                                                                                                                                               gtgtgtCTGTTGGTACGCGTGGCGCATCCAAAACCTATGACCACATGATCATTCGCGGCTTTGCGGAAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGttt                                                                                                     <  4:60453/275‑1 (MQ=255)
                                                                                                                                                               gtgtCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGtat                                                                                                                                                                                                                                   >  6:460492/1‑149 (MQ=255)
                                                                                                                                                                tgtCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGtata                                                                                                                                                                                                                                  >  6:219448/1‑149 (MQ=255)
                                                                                                                                                                 gtCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCCCTGCAACTTcag                                                                                                                                                                                                                                                            >  5:145641/1‑120 (MQ=255)
                                                                                                                                                                  tctcTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCgg                                                                                                                                                                                                                                                 <  4:214811/132‑1 (MQ=255)
                                                                                                                                                                  tctcTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATg                                                                                                                                                                                                                                >  6:857847/1‑149 (MQ=255)
                                                                                                                                                                   ctctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGc                                                                                                                                                                                                                               <  7:673166/149‑1 (MQ=255)
                                                                                                                                                                    tctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCCCTGCAACTTcag                                                                                                                                                                                                                                                            <  7:719884/119‑3 (MQ=255)
                                                                                                                                                                    tctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAg                                                                                                                                                                                                                                                                       <  5:1156209/108‑1 (MQ=255)
                                                                                                                                                                    tctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCt                                                                                                                                                                                                                              >  8:1015930/1‑149 (MQ=255)
                                                                                                                                                                    tctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCt                                                                                                                                                                                                                              >  5:1081634/1‑149 (MQ=255)
                                                                                                                                                                    tctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCt                                                                                                                                                                                                                              >  5:753081/1‑149 (MQ=255)
                                                                                                                                                                     ctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTg                                                                                                                                                                                                                             <  5:519018/149‑1 (MQ=255)
                                                                                                                                                                     ctGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTg                                                                                                                                                                                                                             <  8:1414647/149‑1 (MQ=255)
                                                                                                                                                                         tGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAAc                                                                                                                                                                                                                         <  5:139649/149‑1 (MQ=255)
                                                                                                                                                                              cgcgTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATg                                                                                                                                                                                                                                                    <  2:138153/117‑1 (MQ=255)
                                                                                                                                                                                  tGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGTTCa                                                                                                                                                                                                                                              >  1:14732/1‑119 (MQ=255)
                                                                                                                                                                                  tGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGaaa                                                                                                                                                                                                                <  5:844295/149‑1 (MQ=255)
                                                                                                                                                                                      gcATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGa                                                                                                                                                                                                                                          <  3:14732/119‑1 (MQ=255)
                                                                                                                                                                                      gcATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTAt                                                                                                                                                                                                            <  7:1104389/149‑1 (MQ=255)
                                                                                                                                                                                        aTCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGc                                                                                                                                                                                                          <  6:1448520/149‑1 (MQ=255)
                                                                                                                                                                                        aTCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCACTGAAATTATGc                                                                                                                                                                                                          <  8:793269/149‑1 (MQ=255)
                                                                                                                                                                                         tCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCCACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCg                                                                                                                                                                                                         >  7:1033408/1‑149 (MQ=255)
                                                                                                                                                                                           cAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTg                                                                                                                                                                                                       >  5:661534/1‑149 (MQ=255)
                                                                                                                                                                                            aaCACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTgg                                                                                                                                                                                                      >  5:96761/1‑149 (MQ=255)
                                                                                                                                                                                            aaCACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTgg                                                                                                                                                                                                      >  5:240265/1‑149 (MQ=255)
                                                                                                                                                                                                ccTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCg                                                                                                                                                                                                  <  6:1367835/149‑1 (MQ=255)
                                                                                                                                                                                                ccTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCg                                                                                                                                                                                                  >  8:1334563/1‑149 (MQ=255)
                                                                                                                                                                                                 cTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTg                                                                                                                                                                                                                             <  6:933479/121‑1 (MQ=255)
                                                                                                                                                                                                 cTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGc                                                                                                                                                                                                          >  6:1174295/1‑140 (MQ=255)
                                                                                                                                                                                                 cTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGc                                                                                                                                                                                                          <  5:1174295/140‑1 (MQ=255)
                                                                                                                                                                                                    tGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTTACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTCCGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGCCCACCGCACCGCTGAAAGAAGTTCAGTTTAAAGCCGCTACTTACACTCTGTTCCAGACTGGTATTg                                                                >  2:251188/1‑275 (MQ=255)
                                                                                                                                                                                                    tGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAATTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGGACTGACAGCCTGTTCCAGCCTGGTTTTg                                                                >  2:100334/1‑275 (MQ=255)
                                                                                                                                                                                                      accaccTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTcc                                                                                                                                                                                            >  8:1217627/1‑149 (MQ=255)
                                                                                                                                                                                                        caccTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGt                                                                                                                                                                                          >  7:301489/1‑149 (MQ=255)
                                                                                                                                                                                                          ccTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGt                                                                                                                                                                                                                                     <  4:374649/104‑1 (MQ=255)
                                                                                                                                                                                                          ccTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGc                                                                                                                                                                                        <  8:823993/149‑1 (MQ=255)
                                                                                                                                                                                                          ccTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTg                                                                                                                                                        >  2:377755/1‑181 (MQ=255)
                                                                                                                                                                                                           cTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCt                                                                                                                                                                                       >  6:736034/1‑149 (MQ=255)
                                                                                                                                                                                                           cTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCt                                                                                                                                                                                       >  8:151380/1‑149 (MQ=255)
                                                                                                                                                                                                              aTCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTa                                                                                                                                                                                    <  6:512359/149‑1 (MQ=255)
                                                                                                                                                                                                                cATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCt                                                                                                                                                                                       <  7:83754/144‑1 (MQ=255)
                                                                                                                                                                                                                cATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACg                                                                                                                                                                                  <  7:489947/149‑1 (MQ=255)
                                                                                                                                                                                                                         cTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCa                                                                                                                                                                         <  8:476267/149‑1 (MQ=255)
                                                                                                                                                                                                                         cTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCa                                                                                                                                                                         >  7:1384731/1‑149 (MQ=255)
                                                                                                                                                                                                                               ggCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTg                                                                                                                                                                   >  8:629605/1‑149 (MQ=255)
                                                                                                                                                                                                                                 cAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggc                                                                                                                                                                 <  5:608402/149‑1 (MQ=255)
                                                                                                                                                                                                                                  aGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggcg                                                                                                                                                                >  6:1305227/1‑149 (MQ=255)
                                                                                                                                                                                                                                   gAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTCGCAGGGCAACTTCTATAACGATGCGGTCGTTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggcgg                                                                                                                                                               >  5:591990/1‑149 (MQ=255)
                                                                                                                                                                                                                                      ggCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGt                                                                                                                                                                                                        >  1:20085/1‑105 (MQ=255)
                                                                                                                                                                                                                                       gCCAAAGCTAAAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGGATGATGACGGTGTTTa                              <  2:102318/274‑1 (MQ=255)
                                                                                                                                                                                                                                       gCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTg                                                                                                                                                           >  6:57418/1‑149 (MQ=255)
                                                                                                                                                                                                                                         cAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGtt                                                                                                                                                         <  8:760485/149‑1 (MQ=255)
                                                                                                                                                                                                                                          aaaGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTg                                                                                                                                                        >  5:830008/1‑149 (MQ=255)
                                                                                                                                                                                                                                              ccAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAata                                                                                                                                                    <  5:20284/149‑1 (MQ=255)
                                                                                                                                                                                                                                              ccAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAata                                                                                                                                                    >  5:492627/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATg                                                                                                                                                  >  7:752005/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aaTAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGt                                                                                                                                                <  5:304327/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTc                                                                                                                                               <  6:879017/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    tAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGt                                                                                                                                                                       >  8:431675/1‑124 (MQ=255)
                                                                                                                                                                                                                                                    tAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATTTGGTCa                                                                                                                                              >  6:522475/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    tAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCa                                                                                                                                              >  8:528786/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    tAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCa                                                                                                                                              >  5:367282/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    tAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTAATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGt                                                                                                                                                                       <  7:431675/124‑1 (MQ=255)
                                                                                                                                                                                                                                                        tATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCaa                                                                                                                                          >  8:695194/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        tATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCaa                                                                                                                                          >  7:208345/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         aTCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggc                                                                                                                                                                 <  7:630342/125‑1 (MQ=255)
                                                                                                                                                                                                                                                         aTCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAg                                                                                                                                         >  7:727608/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           cTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCg                                                                                                                                       <  5:544214/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             gAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTc                                                                                                                                     >  5:1053248/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              aaTGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTcc                                                                                                                                    <  7:922600/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                tGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGa                                                                                                                                  <  8:1397564/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTcc                                                                                                                                                                                            >  7:1401491/1‑89 (MQ=255)
                                                                                                                                                                                                                                                                    cTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGaccac                                                                                                                              <  6:1053248/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGaccacc                                                                                                                             >  8:1141558/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      gAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTggcggc                                                                                                                                                              <  2:293223/115‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCg                                                                                                                            <  6:311707/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGc                                                                                                                      <  5:224152/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                      
GGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGGATGATGACGGTGTTTACTCTTATCGCCTGACCGGTCTTGCGCGT  >  NC_000913/167647‑168179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: