Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 5.2% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT 5.2% 665.3 / 17.9 189I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (82/97);  new base T (6/4);  total (88/101)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01

Only 100 of 194 total aligned reads displayed.

TCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTG  >  NC_000913/2081442‑2081905
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                         
tCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccc                                                                                                                                                                                               >  2:74216/1‑275 (MQ=255)
     ggTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTAGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATTTTTTCTTTATCGCCATAACAGATGCGTTCCGTTCCGACCTTTTTCCCCCGGTGCAAAATTTTCACCGTAGGAAAGTTTTGTTGCCCCCAAc                                                                                                                                                                                          >  4:322186/1‑275 (MQ=255)
         aCCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGc                                                                                                                                                                                        >  2:110456/1‑273 (MQ=255)
                                                    cAATTTCCCCTACCCGCGGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCGTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                                                                                           <  2:31231/275‑1 (MQ=255)
                                                                 ccGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCAc                                                                                                                                <  4:74216/273‑1 (MQ=255)
                                                                                                  tttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATGCCCATACCAGTTGCGTTCCTTTCCGACCTTTTCCCCCCGCTGCaaaa                                                                                                                                                                                                                           >  5:1249157/1‑149 (MQ=255)
                                                                                                  tttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                                                                                           >  8:308777/1‑149 (MQ=255)
                                                                                                    tACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt                                                                                                                                                                                                                         <  7:51131/149‑1 (MQ=255)
                                                                                                        aTCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                                                                                                                                                     >  5:942325/1‑149 (MQ=255)
                                                                                                            atggTATATTTGGATTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTATTCCATTACCAGGTCTATATTTTATTTATCGCAATACCAGATGAGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgt                                                                                                                                                                                                                 <  6:352205/147‑1 (MQ=255)
                                                                                                            aGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgt                                                                                                                                                                                                                 <  6:622892/149‑1 (MQ=255)
                                                                                                             gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                                                                                                <  6:602386/149‑2 (MQ=255)
                                                                                                             gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                                                                                                <  5:1383716/149‑2 (MQ=255)
                                                                                                             gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                                                                                                                                       >  6:1353044/1‑142 (MQ=255)
                                                                                                             gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                                                                                                                                       <  5:1353044/142‑1 (MQ=255)
                                                                                                               gTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgtagt                                                                                                                                                                                                              >  5:894952/1‑149 (MQ=255)
                                                                                                                    tttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGt                                                                                                                                                                                                         >  8:399290/1‑149 (MQ=255)
                                                                                                                     ttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCTCCCAACGCAGTAATTTTCTTGCCTCTCCCTCCCGACCTTTTGCACTGTCAGCCCCCTTTACCACTGCAATGAGACGACGACGCCCCNNNACCGCCGAACAAAGGAGAGAAAAccccc                                                                           >  3:74360/1‑272 (MQ=255)
                                                                                                                      tGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGtgt                                                                                                                                                                                                       <  8:184963/149‑3 (MQ=255)
                                                                                                                       ggCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGtttt                                                                                                                                                                                                      <  7:614486/149‑1 (MQ=255)
                                                                                                                        gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                                                                                                                                                     <  6:408305/149‑1 (MQ=255)
                                                                                                                          tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                                                                                                                                                   >  6:1023569/1‑149 (MQ=255)
                                                                                                                          tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                                                                                                                                                   >  6:528346/1‑149 (MQ=255)
                                                                                                                            tGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGc                                                                                                                                                                                                 <  7:70758/149‑1 (MQ=255)
                                                                                                                            tGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAAt                                                                                         <  4:98898/253‑1 (MQ=255)
                                                                                                                             gATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGATCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcc                                                                                                                                                                                                <  7:996014/149‑1 (MQ=255)
                                                                                                                             gATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcc                                                                                                                                                                                                <  8:707768/149‑1 (MQ=255)
                                                                                                                              atatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccc                                                                                                                                                                                               <  7:598165/149‑1 (MQ=255)
                                                                                                                                   tgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAAc                                                                                                                                                                                          >  5:210791/1‑149 (MQ=255)
                                                                                                                                    ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACg                                                                                                                                                                                         >  7:1401970/1‑149 (MQ=255)
                                                                                                                                    ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACg                                                                                                                                                                                         >  7:802315/1‑149 (MQ=255)
                                                                                                                                      aaTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCa                                                                                                                                                                                       <  6:1196870/149‑1 (MQ=255)
                                                                                                                                              cTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAtttt                                                                                                                                                                               >  6:1235250/1‑149 (MQ=255)
                                                                                                                                               tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTc                                                                                                                                                                              >  8:337314/1‑149 (MQ=255)
                                                                                                                                                ttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGACGAAGCAATGAGCTTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGcc                                               >  2:37155/1‑275 (MQ=255)
                                                                                                                                                tataaggtccagaTCCAGAACTCTTGTTCCGTTCCCATGTCTATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCTCGGTGCAAAATTTTCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCATGACCTTTTGCACTTTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGc                                                <  2:253422/262‑1 (MQ=255)
                                                                                                                                                   ggTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                                                                                                                                          <  6:210791/149‑1 (MQ=255)
                                                                                                                                                   ggTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                                                                                                                                          <  6:1050969/149‑1 (MQ=255)
                                                                                                                                                    gTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                                                                                                                                         >  7:763123/1‑149 (MQ=255)
                                                                                                                                                    gTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                                                                                                                                         >  7:1008156/1‑149 (MQ=255)
                                                                                                                                                      aGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                                                                                                                                                                       <  5:39333/149‑1 (MQ=255)
                                                                                                                                                          ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                                                                                                                                                    >  8:116275/1‑100 (MQ=255)
                                                                                                                                                          ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                                                                                                                                                    <  7:116275/100‑1 (MQ=255)
                                                                                                                                                           cATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCt                                                                                                                                                                  <  8:136892/149‑1 (MQ=255)
                                                                                                                                                             tCCAGAACTCTTGTTCCGTTCCCAGGTCTATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACATTTTTCCCACGGTGCAAAATTTGCACCGAAGTAATGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCtaa                                                                                                                                                                >  6:602351/1‑147 (MQ=255)
                                                                                                                                                             tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                                                                                                                                                >  7:489616/1‑149 (MQ=255)
                                                                                                                                                              ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCAc                                                                                                                                                               <  5:820371/149‑1 (MQ=255)
                                                                                                                                                                aGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGa                                                                                                                                                             >  5:498438/1‑149 (MQ=255)
                                                                                                                                                                 gAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc                                                                                                                                                            >  6:141089/1‑149 (MQ=255)
                                                                                                                                                                  aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAcc                                                                                                                                                           <  8:956422/149‑1 (MQ=255)
                                                                                                                                                                      ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt                                                                                                                                                       <  7:815829/149‑1 (MQ=255)
                                                                                                                                                                       ttGTTCCGTTCCCAGGTCGATATTTTCTTTTTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                                                                                                                                             >  1:61819/1‑110 (MQ=255)
                                                                                                                                                                       ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCATGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAAGGAGATTAAACCAGGCACCAGCAGTATGACCCGTTTTCAGGCCGTCAACATTCCTgtcacttata                         >  4:253422/1‑265 (MQ=255)
                                                                                                                                                                       ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTg                                                                                                                                                 >  4:178845/1‑154 (MQ=255)
                                                                                                                                                                       ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCa                                                                                                          >  2:353441/1‑193 (MQ=255)
                                                                                                                                                                         gttccgttcaCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                                                                                                    >  6:1118546/1‑149 (MQ=255)
                                                                                                                                                                          ttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                                                                                            <  8:544722/124‑1 (MQ=255)
                                                                                                                                                                          ttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTGTGCAc                                                                                                                                                   >  6:1147872/1‑149 (MQ=255)
                                                                                                                                                                           tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                                                                                                                                             <  2:178845/154‑1 (MQ=255)
                                                                                                                                                                                 cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGc                                                                                                                                            <  5:1057685/149‑1 (MQ=255)
                                                                                                                                                                                   ccggtccgtatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGGAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGt            <  4:37155/267‑1 (MQ=255)
                                                                                                                                                                                   cAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGccc                                                                                                                                          >  5:977292/1‑149 (MQ=255)
                                                                                                                                                                                        cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGccc                                                                                                                                          <  5:1209589/144‑1 (MQ=255)
                                                                                                                                                                                         gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                                                                                                    >  8:1402968/1‑149 (MQ=255)
                                                                                                                                                                                            atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa                                                                                                                                 <  7:381197/149‑1 (MQ=255)
                                                                                                                                                                                            atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa                                                                                                                                 <  6:856208/149‑1 (MQ=255)
                                                                                                                                                                                               ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTg                                                                                                                              <  8:191610/149‑1 (MQ=255)
                                                                                                                                                                                                tCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCCCGACCTTTTGCACTGNCAGCCCCCNTTACCCCTGCAATGAGACGACCCTGCCCATCCACAGCCTaa                                                                                              >  3:24416/1‑180 (MQ=255)
                                                                                                                                                                                                  tttATCGCCATACCAGATGCGTTCCGTTCTGACCTTTTTCGCGCGAAGCTTGATTTGCACCGTAGTTTTGTTTTGTTCCACCCAACAAAGTAATTTCCTTTCCTTTTCCTCCTTTCCTTTTGCCATGTCCCACTCCATTACCACTGCaa                                                                                                                           >  5:581737/1‑149 (MQ=255)
                                                                                                                                                                                                  tttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCaa                                                                                                                           <  8:1036531/149‑1 (MQ=255)
                                                                                                                                                                                                    tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCACACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCaa                                                                                          <  1:24416/180‑1 (MQ=255)
                                                                                                                                                                                                     aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATga                                                                                                                        >  8:1251465/1‑149 (MQ=255)
                                                                                                                                                                                                       cGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATgaga                                                                                                                      <  5:141089/149‑1 (MQ=255)
                                                                                                                                                                                                             aCCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGc                                                                                                                >  5:1434721/1‑149 (MQ=255)
                                                                                                                                                                                                               cAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg                                                                                                              <  7:308777/149‑1 (MQ=255)
                                                                                                                                                                                                                aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGc                                                                                                             >  8:130195/1‑149 (MQ=255)
                                                                                                                                                                                                                 gATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                                                                                                                                             >  4:12601/1‑68 (MQ=255)
                                                                                                                                                                                                                 gATGCGTTCCGTTCCGACCTTTCTCCCACGGTGCAAAATTTGCACCGTAGTGAAGTTTTTTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGGTGcc                                                                                                            >  8:909997/1‑149 (MQ=255)
                                                                                                                                                                                                                  aTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGccc                                                                                                           <  7:1372224/149‑1 (MQ=255)
                                                                                                                                                                                                                     cgttccgttcCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACg                                                                                                                                                                                         <  2:12601/68‑1 (MQ=255)
                                                                                                                                                                                                                      gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCt                                                                                                       <  8:829995/149‑1 (MQ=255)
                                                                                                                                                                                                                      gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCt                                                                                                       >  6:783134/1‑149 (MQ=255)
                                                                                                                                                                                                                          cgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAg                                                                                                   >  7:691325/1‑149 (MQ=255)
                                                                                                                                                                                                                               cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGaa                                                                                              <  6:894952/149‑1 (MQ=255)
                                                                                                                                                                                                                                gACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                                                                                                    <  4:361090/94‑1 (MQ=255)
                                                                                                                                                                                                                                gACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAg                                                                                             <  8:1346669/149‑1 (MQ=255)
                                                                                                                                                                                                                                gACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAg                                                                                             >  8:657814/1‑149 (MQ=255)
                                                                                                                                                                                                                                    tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAAt                                                                                         >  6:248370/1‑149 (MQ=255)
                                                                                                                                                                                                                                    tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAAt                                                                                         <  7:562244/149‑1 (MQ=255)
                                                                                                                                                                                                                                      tttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                                                                                       <  7:1067463/149‑1 (MQ=255)
                                                                                                                                                                                                                                      tttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                                                                                       <  7:1316793/149‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgag                                                                                      <  8:61158/149‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgag                                                                                      <  7:932942/149‑1 (MQ=255)
                                                                                                                                                                                                                                          ccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGAtt                                                                                   >  8:440830/1‑149 (MQ=255)
                                                                                                                                                                                                                                           taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa                                                                                  >  8:24272/2‑149 (MQ=255)
                                                                                                                                                                                                                                           taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa                                                                                  >  8:1358821/2‑149 (MQ=255)
                                                                                                                                                                                                                                           cACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa                                                                                  >  5:992987/1‑149 (MQ=255)
                                                                                                                                                                                                                                              ggTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAAc                                                                               >  7:1227871/1‑149 (MQ=255)
                                                                                                                                                                                                                                               gTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAAcc                                                                              <  6:184584/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAc                                                                        <  7:190154/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                         
TCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTG  >  NC_000913/2081442‑2081905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑