Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,315 C→G 6.2% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3150CG 6.2% 673.4 / 28.3 193intergenic (+28/+75)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (97/83);  new base G (3/9);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 7.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

Only 100 of 193 total aligned reads displayed.

CAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAAT  >  NC_000913/2286098‑2286549
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
                                                      ttAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTa                                                                                                                                                                             >  3:35113/1‑228 (MQ=255)
                                                          cGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtttt                                                                                                                                                                         <  1:35113/228‑1 (MQ=255)
                                                                       ttGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACgg                                                                                                                                                                                                                                           >  7:148746/1‑149 (MQ=255)
                                                                        tGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                                                                                                                                                                          <  6:435391/149‑1 (MQ=255)
                                                                         gCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCGCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggtt                                                                                                                                                                                                                                         <  8:1216697/149‑1 (MQ=255)
                                                                          cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                                                                                                                                                                        <  6:1214887/149‑1 (MQ=255)
                                                                          cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                                                                                                                                                                        <  6:1469588/149‑1 (MQ=255)
                                                                           aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttgg                                                                                                                                                                                                                                       >  8:1244682/1‑149 (MQ=255)
                                                                            aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggt                                                                                                                                                                                                                                      <  7:12176/149‑1 (MQ=255)
                                                                             tCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                                                                                                                     <  7:880524/149‑1 (MQ=255)
                                                                              cGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttt                                                                                                                                                                                                                                    <  5:678283/149‑1 (MQ=255)
                                                                               ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttt                                                                                                                                                                                                                                   >  7:511696/1‑149 (MQ=255)
                                                                                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                                                                                                                     <  7:611209/146‑1 (MQ=255)
                                                                                  gtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttc                                                                                                                                                                                                                                <  8:1275996/149‑2 (MQ=255)
                                                                                  gtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                                                                                                                                <  7:382671/149‑1 (MQ=255)
                                                                                   tgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                                                                                                                                                               <  8:451817/149‑1 (MQ=255)
                                                                                   tgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                                                                                                                                                               <  5:251495/149‑1 (MQ=255)
                                                                                   tgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCCATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCCGACCACACCCGCCCGACTTTTCCACGGCGATTCTTCCCCTTCCCCATTGCCAtc                                                                                                 >  4:300590/1‑274 (MQ=255)
                                                                                       aaaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATc                                                                                                                                                                                                                           >  8:96546/1‑149 (MQ=255)
                                                                                           ggTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCa                                                                                                                                                                                                                       >  6:1474692/1‑149 (MQ=255)
                                                                                            gtagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaa                                                                                                                                                                                                                      <  8:1336991/149‑1 (MQ=255)
                                                                                             tagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                                                                                                                                                                     <  5:786392/149‑1 (MQ=255)
                                                                                               gtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTATCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatta                                                                                                                                                                                                                   >  7:1121917/1‑149 (MQ=255)
                                                                                                taTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACg                                                                                                                                                                                                                                            >  8:88155/1‑123 (MQ=255)
                                                                                                taTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaa                                                                                                                                                                                                                  <  5:463276/149‑1 (MQ=255)
                                                                                                taTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaa                                                                                                                                                                                                                  >  6:25642/1‑149 (MQ=255)
                                                                                                 atacacagcCACAAGTCGTCACGAAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCTGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAAt                                                                                   <  2:132516/270‑1 (MQ=255)
                                                                                                 aTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaat                                                                                                                                                                                                                 <  8:1312876/149‑1 (MQ=255)
                                                                                                  ttAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                                                                                                                                                                <  7:1170102/149‑1 (MQ=255)
                                                                                                  ttAGCAGCAACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTGCAGTTGGATTTTTTATATCTGCaattaatt                                                                                                                                                                                                                <  6:1362832/149‑1 (MQ=255)
                                                                                                    agcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                                                                                                              >  8:1349778/1‑149 (MQ=255)
                                                                                                    agcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGAc                                                                                <  2:300590/275‑1 (MQ=255)
                                                                                                     gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGa                                                                                                                                                                                                             <  5:1301938/149‑1 (MQ=255)
                                                                                                       agcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAAACCTACCGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACATACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCATAACGTCCAGTCCACAACCCCCTGAGTATTCCACGCCGATTCTACCCCTGCCCCATTGACATAGCCGACTGACaccatcacgg                                                                             >  4:71982/1‑266 (MQ=255)
                                                                                                        gcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaa                                                                                                                                                                                                          >  8:514363/1‑149 (MQ=255)
                                                                                                         ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGCCCAAAAATCCCAAGAACAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                                                                                                                         <  5:1345185/149‑1 (MQ=255)
                                                                                                         ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                                                                                                                         >  5:1198042/1‑149 (MQ=255)
                                                                                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                                                                                                                                                                       >  7:24358/1‑149 (MQ=255)
                                                                                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                                                                                                                                                               <  2:191537/189‑1 (MQ=255)
                                                                                                            cGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTACACGGCGATTCTCCCCCTCCGCCCTTGCCATAGCTGACTGACAAATGACCGCTTCAc                                                                        >  2:26950/1‑275 (MQ=255)
                                                                                                                  ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg                                                                                                                                                                                                >  8:1022759/1‑149 (MQ=255)
                                                                                                                           cgaggCCTGTAAGCGCACCGTAATGGGGTGTCGGGGGTCGAAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTCACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGAt                                                         <  2:71982/271‑1 (MQ=255)
                                                                                                                            gcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca                                                                                                                                                                                      <  5:1397707/149‑1 (MQ=255)
                                                                                                                            gcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca                                                                                                                                                                                      >  7:753434/1‑149 (MQ=255)
                                                                                                                              aGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCTAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc                                                                                                                                                                                    <  7:993570/149‑1 (MQ=255)
                                                                                                                               gCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                                                                                                                                                                   >  5:749368/1‑149 (MQ=255)
                                                                                                                                ccTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCt                                                                                                                                                                                  <  8:598307/149‑1 (MQ=255)
                                                                                                                                  tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                                                                                                                >  8:1054447/1‑149 (MQ=255)
                                                                                                                                  tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                                                                                                                <  5:29795/149‑1 (MQ=255)
                                                                                                                                  tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                                                                                                                >  5:950369/1‑149 (MQ=255)
                                                                                                                                   ggTAGCGCACCGTCTTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGtt                                                                                                                                                                               >  7:112831/1‑149 (MQ=255)
                                                                                                                                     tAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac                                                                                                                                               >  2:235932/1‑179 (MQ=255)
                                                                                                                                         gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtttt                                                                                                                                                                         >  8:164804/1‑149 (MQ=255)
                                                                                                                                          cACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                                                                                                        <  5:1474692/149‑1 (MQ=255)
                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCTGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                       <  8:2336/149‑1 (MQ=255)
                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                       <  8:613271/149‑1 (MQ=255)
                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                       <  5:383329/149‑1 (MQ=255)
                                                                                                                                             cGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGt                                                                                                                                                                     >  5:108056/1‑149 (MQ=255)
                                                                                                                                               tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAACAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                                                                                                                                                                   >  7:1120193/1‑149 (MQ=255)
                                                                                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                                                                                                                 >  6:617423/1‑149 (MQ=255)
                                                                                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                                                                                                                 >  5:1028122/1‑149 (MQ=255)
                                                                                                                                                    gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                                                                                                                                                              >  8:1113206/1‑149 (MQ=255)
                                                                                                                                                     ggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATGCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGaa                                                                                                                                                             <  6:1424566/149‑1 (MQ=255)
                                                                                                                                                         tCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc                                                                                                                                                         <  7:735635/149‑1 (MQ=255)
                                                                                                                                                         tCGGGGGTCGGAGGTTCAAATAATCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATGCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTATGTTATCATAACTTCCAGACCAAACCCGCCTGAGTATTCCACGACGATTCTACACCTGCCCCGTTCCCATCGCTGACTGACAAATCACAGATTAGCGCCGGGGAAAACGATGACATTTAACAAGCCTGaaaacagacacag                           >  4:43855/1‑266 (MQ=255)
                                                                                                                                                            ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTAGCAGAACGTCCAg                                                                                                                                                      >  5:1171552/1‑149 (MQ=255)
                                                                                                                                                             gggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGAt                                                         <  4:217809/241‑1 (MQ=255)
                                                                                                                                                                 cGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTcc                                                                                                                                >  4:359012/1‑166 (MQ=255)
                                                                                                                                                                   gAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttt                                                                                                                                                                                                                                    <  6:1097419/64‑1 (MQ=255)
                                                                                                                                                                   gAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttt                                                                                                                                                                                                                                    >  5:1097419/1‑64 (MQ=255)
                                                                                                                                                                    aGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACAcc                                                                                                                                              <  8:753434/149‑1 (MQ=255)
                                                                                                                                                                     ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACAccc                                                                                                                                             <  7:121944/149‑1 (MQ=255)
                                                                                                                                                                       ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                                                                                                           <  5:86878/149‑1 (MQ=255)
                                                                                                                                                                       ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                                                                                                           >  6:360026/1‑149 (MQ=255)
                                                                                                                                                                        tCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGcc                                                                                                                                          >  7:643683/1‑149 (MQ=255)
                                                                                                                                                                         cAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                                                                                                                         <  6:1028122/149‑1 (MQ=255)
                                                                                                                                                                           aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                                                                                                                       <  8:1071633/149‑1 (MQ=255)
                                                                                                                                                                            aTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                                                                                                                      >  8:387227/1‑149 (MQ=255)
                                                                                                                                                                                tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                       >  6:531914/1‑112 (MQ=255)
                                                                                                                                                                                tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                       <  5:531914/112‑1 (MQ=255)
                                                                                                                                                                                 gtcatgacGATCAGAAATGCCAAAAAAAAACCAACTCTCACGGTAGGGGTACGTAAACCTGCAATTAATTCGATAAAAAGACTGTGACACACCACGGCCGGTTTATTTTCGGTTATGAGAACGTCCAGACCACAACCGCATGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGGCTGACAAATGACCGCTTAACGTAGGGAGAAAAGAATACCTTATAACAGCCTGAAACACGCCAATGGTACCAGACCACATATTATTAAAt  >  4:105693/8‑275 (MQ=255)
                                                                                                                                                                                 ctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTc                                                                                                                                 <  5:617423/149‑1 (MQ=255)
                                                                                                                                                                                    gTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCAc                                                                                                                              >  7:468946/1‑149 (MQ=255)
                                                                                                                                                                                        cGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCg                                                                                                                          >  7:1107799/1‑149 (MQ=255)
                                                                                                                                                                                         gACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGa                                                                                                                         >  8:1157420/1‑149 (MQ=255)
                                                                                                                                                                                              aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACgg                                                                                                                            <  2:381961/141‑1 (MQ=255)
                                                                                                                                                                                                    ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTg                                                                                                              >  8:993413/1‑149 (MQ=255)
                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGaca                                                                                                                                                                                             >  6:1169090/1‑69 (MQ=255)
                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGaca                                                                                                                                                                                             <  5:1169090/69‑1 (MQ=255)
                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGaa                                                                                                                                                             >  6:874907/1‑101 (MQ=255)
                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                                                                                                             >  7:579968/1‑149 (MQ=255)
                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                                                                                                             >  6:810566/1‑149 (MQ=255)
                                                                                                                                                                                                           aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt                                                                                                       >  6:984265/1‑149 (MQ=255)
                                                                                                                                                                                                           aaaaCCAACCGTAAGGCTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGCGACACATAACAGCCTGTTTATTTTCTGTTATCAGAACGTTCAGACCACAGCCGCCTGAGTATTCCACGGCGATTCTACCCCCGCGCCAtt                                                                                                       >  7:907338/1‑149 (MQ=255)
                                                                                                                                                                                                              accaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc                                                                                                    >  7:554550/1‑149 (MQ=255)
                                                                                                                                                                                                                  accCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAg                                                                                                <  8:543683/149‑1 (MQ=255)
                                                                                                                                                                                                                    ccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGct                                                                                              >  7:1326141/1‑149 (MQ=255)
                                                                                                                                                                                                                     cTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc                                                                                                    <  8:1282611/142‑1 (MQ=255)
                                                                                                                                                                                                                     cTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc                                                                                                    >  7:1282611/1‑142 (MQ=255)
                                                                                                                                                                                                                      ttACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                                                                                                                       >  7:277965/1‑106 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
CAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAAT  >  NC_000913/2286098‑2286549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: