Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,318,095 C→G 100% L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG99.4% 677.5 / ‑4.6 185L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (85/97);  total (87/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGT  >  NC_000913/2317822‑2318342
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGCTAATCGCCCTCCGCGCGAACATGCAAACCTATACAGACGGCAACGAGGAATTTTTTGGCGTTACTGAAACGGTGGgaga                                                                                                                                                                                                                                            >  4:102484/1‑275 (MQ=255)
                                    cGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCGCCAGGAAATTACGCTATACGCCCGTTCCGACCAGGAAGAAGGGATCAAACAAGCGCACAACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATATAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAGCGCATCGGGt                                                                                                                                                                                                                    <  2:102484/275‑1 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCCTCGGGTCGCCCTTTCAGGc                                                                                                                                                                                                       >  1:14083/1‑275 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCCGGAAGAAGGGATTAAACAACCGCACCACTTCTTTCGCCGGTATCCCCACTCCGGTATCGCGAACACGGATAGGACGATAATCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGGTACCGGTGAATTTTATGGCGTTAACCAAAAGGTTGGAGATGACCTTCGGTAAACGAAGTGGGTCTCCATGAAAGGc                                                                                                                                                                                                       >  4:310814/1‑275 (MQ=255)
                                                                aGTTTTTCACCAATCGCCAGACCCAGCCCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                        <  2:112875/275‑1 (MQ=255)
                                                                       cacaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTgg                                                                                                                                                                                                 >  4:272285/1‑259 (MQ=255)
                                                                           aaTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                             <  2:272285/259‑1 (MQ=255)
                                                                                 cagacccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                        <  2:340690/274‑1 (MQ=255)
                                                                                      ccacaccGGTCCCCTGGAAAGTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCt                                                                                                                                                                  <  1:32349/275‑1 (MQ=255)
                                                                                       cagaccGGTCCCCGGGAAATTACGCGGTACGCCCGTTCCGACCGGGAAGAAGGGATCAAACAAGCGCAACACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTa                                                                                                                                                                 <  2:302697/275‑1 (MQ=255)
                                                                                              gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCTCCGGTATCCCCACGCCGGTATCTCGAACACGGATAGATAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGACTTTTCTGGCGTTACTTAACAGGTTGGAGATTCCCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACAt                                                                                                                                                                                            >  2:249309/1‑241 (MQ=255)
                                                                                                                        cGTTCCGAACTGGAAAAAGGGATCAAAAAAGCTCACCACTTCTTTTGCCGGGATCCCCCCGCCGGGATCGCGAACACGTATAGAGAGATAATCGCCATCCGCGCTAACATGCAAAACTATACAGCCGGGATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTgg                                                                                                                                <  4:43594/275‑1 (MQ=255)
                                                                                                                               aCCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATCCAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                       >  6:842312/1‑149 (MQ=255)
                                                                                                                                ccTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAc                                                                                                                                                                                                                                                      <  8:34591/149‑1 (MQ=255)
                                                                                                                                 cTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACa                                                                                                                                                                                                                                                     >  7:496309/1‑149 (MQ=255)
                                                                                                                                 cTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCACCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACa                                                                                                                                                                                                                                                     >  5:1101508/1‑149 (MQ=255)
                                                                                                                                  tGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAg                                                                                                                                                                                                                                                    <  5:895168/149‑1 (MQ=255)
                                                                                                                                  tGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAg                                                                                                                                                                                                                                                    >  5:625455/1‑149 (MQ=255)
                                                                                                                                  tGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAg                                                                                                                                                                                                                                                    <  5:376682/149‑1 (MQ=255)
                                                                                                                                  tGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAg                                                                                                                                                                                                                                                    <  5:120098/149‑1 (MQ=255)
                                                                                                                                    gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                  <  6:1119286/149‑1 (MQ=255)
                                                                                                                                    gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                  >  6:982775/1‑149 (MQ=255)
                                                                                                                                        aagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGga                                                                                                                                                                                                                                              <  8:91579/149‑1 (MQ=255)
                                                                                                                                            gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                                                                                                                                          >  8:1228313/1‑149 (MQ=255)
                                                                                                                                            gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                                                                                                                                          >  7:1299725/1‑149 (MQ=255)
                                                                                                                                            gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                                                                                                                                          >  5:668385/1‑149 (MQ=255)
                                                                                                                                             aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa                                                                                                                                                                                                                                         <  8:40285/149‑1 (MQ=255)
                                                                                                                                             aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa                                                                                                                                                                                                                                         >  8:1107979/1‑149 (MQ=255)
                                                                                                                                               cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                       <  8:1350617/149‑1 (MQ=255)
                                                                                                                                                   cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                                                                                                                                   <  7:1415205/149‑1 (MQ=255)
                                                                                                                                                   cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                                                                                                                                   <  7:1209387/149‑1 (MQ=255)
                                                                                                                                                            cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa                                                                                                                                                                                                                          >  7:1429013/1‑149 (MQ=255)
                                                                                                                                                              cttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATc                                                                                                                                                                                                                        >  6:1253150/1‑149 (MQ=255)
                                                                                                                                                               ttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                                                                                                                                                                                                                       >  6:586529/1‑149 (MQ=255)
                                                                                                                                                                cttttCGCCAGTACACCCACGCCGGTATCGCGAACACGGAGAGAGAGATAATAGCCATCATCGCGAACATGAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGGTGGGGATGACCTGCTGTAAACGCATGGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTGGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGGGATTCATCACTTCACGCGGTGAAAACTCAcg                                                                                        <  4:254964/273‑1 (MQ=255)
                                                                                                                                                                   ttCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                                                                                                                                   <  5:982775/149‑1 (MQ=255)
                                                                                                                                                                          gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt                                                                                                                                                                                                            <  5:586529/149‑1 (MQ=255)
                                                                                                                                                                          gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt                                                                                                                                                                                                            >  7:371385/1‑149 (MQ=255)
                                                                                                                                                                            aTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTaa                                                                                                                                                                                                          <  5:1083605/149‑1 (MQ=255)
                                                                                                                                                                             tCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                         <  6:437867/149‑1 (MQ=255)
                                                                                                                                                                             tCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                         >  8:494529/1‑149 (MQ=255)
                                                                                                                                                                             tCCCCACGCAGGTATCGCGAACTCGGATAGAGAGATAATCGCCATTCGCGCGAACATGCAAAACTATACGGCCATTATCAGTGAATTTTATGGCGTTACTGAACAGGGTGGAGATGACCTGCTGTAAACGAATCGGGGCGCCATTTAAg                                                                                                                                                                                                         <  7:604006/149‑1 (MQ=255)
                                                                                                                                                                               cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc                                                                                                                                                                                                       >  7:668458/1‑149 (MQ=255)
                                                                                                                                                                                ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc                                                                                                                                                                                                      >  6:817370/1‑149 (MQ=255)
                                                                                                                                                                                ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc                                                                                                                                                                                                      >  8:725356/1‑149 (MQ=255)
                                                                                                                                                                                  aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCAc                                                                                                                                                                                                    <  8:668458/149‑1 (MQ=255)
                                                                                                                                                                                  aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCAc                                                                                                                                                                                                    <  6:694190/149‑1 (MQ=255)
                                                                                                                                                                                    gCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTg                                                                                                                                                                                                  >  8:590832/1‑149 (MQ=255)
                                                                                                                                                                                       ggTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGca                                                                                                                                                                                               <  7:1186650/149‑1 (MQ=255)
                                                                                                                                                                                         tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTTGTTCATCACTTCACGCGGTGAAAACTCACCCGGTTCGTTCTTCAACTGTTCCGAt                                                               >  2:86980/1‑275 (MQ=255)
                                                                                                                                                                                           tCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                           >  8:325288/1‑149 (MQ=255)
                                                                                                                                                                                           tCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                           <  8:1429013/149‑1 (MQ=255)
                                                                                                                                                                                              cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGcc                                                                                                                                                                                                                <  7:640475/125‑1 (MQ=255)
                                                                                                                                                                                               gAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGt                                                                                                                                                                                       >  6:230353/1‑149 (MQ=255)
                                                                                                                                                                                               gAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGt                                                                                                                                                                                       <  5:1253150/149‑1 (MQ=255)
                                                                                                                                                                                                aaCACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt                                                                                                                                                                                      <  8:807247/149‑1 (MQ=255)
                                                                                                                                                                                                     ggATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTaaa                                                                                                                                                                                                                              <  7:1073232/104‑1 (MQ=255)
                                                                                                                                                                                                     ggATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATa                                                                                                                                                                                 >  7:462528/1‑149 (MQ=255)
                                                                                                                                                                                                       aTAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGGTCCTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCGATTTGAGGCCACAGGCACATCCGGTTCAATaaa                                                                                                                                                                               <  7:992290/149‑1 (MQ=255)
                                                                                                                                                                                                        tAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                              <  8:614147/149‑1 (MQ=255)
                                                                                                                                                                                                         agagagATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGc                                                                                                                                                                             >  8:869577/1‑149 (MQ=255)
                                                                                                                                                                                                               aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACa                                                                                                                                                                       <  5:1024064/149‑1 (MQ=255)
                                                                                                                                                                                                                tAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                                                                                                                                      >  8:1249614/1‑149 (MQ=255)
                                                                                                                                                                                                                tAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                                                                                                                                      <  7:304921/149‑1 (MQ=255)
                                                                                                                                                                                                                 aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                        >  2:382584/1‑146 (MQ=255)
                                                                                                                                                                                                                   tCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                  <  4:345297/70‑1 (MQ=255)
                                                                                                                                                                                                                   tCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGcc                                                                                                                                                                   >  5:1225190/1‑149 (MQ=255)
                                                                                                                                                                                                                     gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgaga                                                                                                                                                                                                                                            >  2:54523/1‑74 (MQ=255)
                                                                                                                                                                                                                      ccATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc                                                                                                                                                                                                       >  5:281821/1‑110 (MQ=255)
                                                                                                                                                                                                                      ccATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaaa                                                                                                                                                                               <  5:1461163/134‑1 (MQ=255)
                                                                                                                                                                                                                      ccATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaaa                                                                                                                                                                               >  6:1461163/1‑134 (MQ=255)
                                                                                                                                                                                                                      ccATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                                                                                                                                      <  7:1130519/143‑1 (MQ=255)
                                                                                                                                                                                                                      ccATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTaa                                                                                                                                                                >  6:1238941/1‑149 (MQ=255)
                                                                                                                                                                                                                            gcgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCtt                                                                                                                                                          <  6:1173938/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                                                                                                                                   <  6:64961/83‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                 >  5:238410/1‑149 (MQ=255)
                                                                                                                                                                                                                                     atgaaaaCTATACAGCCGGGTGTGGTGAATGTTGTGGCTGTACTGAACAGGTGGGAGGTGGCCTGGTGTAAAGGCATCGGTTCGCGATTTAAGGCCACTGGGACGTCCGGTTCACTAGAGCAGTACAAGCGTAACGGCTTGCGTACCACCAGAGGTAAAGATTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAAATTTcaacaa                   <  4:161086/272‑1 (MQ=255)
                                                                                                                                                                                                                                      gCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGGCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccacc                                                                                                                                                >  8:1159771/1‑149 (MQ=255)
                                                                                                                                                                                                                                       cAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGcc                                                                                                                                                                                                                >  8:1456172/1‑84 (MQ=255)
                                                                                                                                                                                                                                        aaaaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGt                                                                                                                                                      <  6:1357280/141‑1 (MQ=255)
                                                                                                                                                                                                                                        aaaaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                                                                                                                              >  6:669546/1‑149 (MQ=255)
                                                                                                                                                                                                                                          aaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTGAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCg                                                                                                                                            >  7:897705/1‑149 (MQ=255)
                                                                                                                                                                                                                                            cTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTGAACGCAGCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAGAAAGCAGTACAAGCCTAACTGCTTGCGTACCATCAGCGGt                                                                                                                                          <  8:50637/149‑1 (MQ=255)
                                                                                                                                                                                                                                            cTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGt                                                                                                                                          >  8:155548/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATa                                                                                                                                     <  6:725091/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGtt                                                                                                                                  <  8:122598/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCACTGATGTGGTTCATCACTTCACGCGGTGAACACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt  >  2:283072/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGAAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATCAATCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTCAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt  >  2:169072/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            ggTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGa                                                                                                                          >  8:220926/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             gTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt                                                                                                                         >  6:474682/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                        >  7:190824/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ttttATGGCGTTACTGAACAGGTTGGCGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGt                                                                                                                    >  8:1040399/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGt                                                                                                                    >  6:168388/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGt                                                                                                                    >  7:332402/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  tcttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGt                                                                                                                    <  6:729387/147‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAg                                                                                                                                                                     <  7:553153/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGtt                                                                                                                   <  5:15745/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtca                                                                                                                 <  6:625455/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                                                                                                                                      >  5:813146/1‑95 (MQ=255)
                                                                                                                                                                                                                                                                              aCTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAc                                                                                                        <  5:714735/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       
GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGT  >  NC_000913/2317822‑2318342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑