Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,415,725 C→A 5.1% L327M (CTG→ATG) ‡ pta → phosphate acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,415,7250CA 5.1% 756.3 / 21.8 215L327M (CTG→ATG) ‡ptaphosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (87/117);  new base A (8/3);  total (95/120)
Fisher's exact test for biased strand distribution p-value = 6.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 217 total aligned reads displayed.

TCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAACTTGCGCGCAAAGCGGGCAAACGTATCGTACTGCCGGAAGGTGACGAACCGCGT  >  NC_000913/2415454‑2415994
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
  gCCCCCGGAATGCGACCATCATCAACGAAGGCTACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGAACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGcc                                                                                                                                                                                                                                                                           <  4:251891/274‑1 (MQ=255)
  gCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGAGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTTCTGGCCTGCCg                                                                                                                                                                                                                                                                          >  2:107327/1‑275 (MQ=255)
  gCCACATGAATGCGACCATCATCAACCAAGGCGACATCAAGACTCGCCGCGTTAAAGTCGTCACTTTCTGAGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTGCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGcc                                                                                                                                                                                                                                                                           <  2:45535/274‑1 (MQ=255)
                aCCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGCCGTAGCAATCGGTTCCCTGCTGCTGCCTGGAGGTTACGAAATGGACGCGCGCCGTTCCAAACTGTTCGCACAGTACTTCGCTACCGGCCTCCCGGTATTTTTgattg                                                                                                                                                                                                                                                             >  4:100146/1‑270 (MQ=255)
                               ggCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTgg                                                                                                                                                                                                                                               >  2:256185/1‑273 (MQ=255)
                                                                                       gccggtccTGGGGCCCTGCCGTGCCGGTTCGCTGCTGGTGACTTCCGCAGGCCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGaa                                                                                                                                                                                      <  2:158947/267‑1 (MQ=255)
                                                                                           aTGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCCCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACCCCTGGCCGACCGCCCTGAGCCTGCAGAGCCTCAACCTGGAATTTCCGGTGTACGCTCACGAACGTaa                                                                                                                                                                                 >  4:36206/1‑274 (MQ=255)
                                                                                                                 gTTCTTGGCTGGTGACGTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGGAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGtt                                                                                                                                                           <  4:293570/275‑1 (MQ=255)
                                                                                                                                cTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCg                                                                                                                                                                                                                                                                          <  6:1408852/149‑1 (MQ=255)
                                                                                                                                cTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGCCCTGCCg                                                                                                                                                                                                                                                                          <  6:807327/149‑1 (MQ=255)
                                                                                                                                 ttCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCgg                                                                                                                                                                                                                                                                         >  8:497986/1‑149 (MQ=255)
                                                                                                                                 ttCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCgg                                                                                                                                                                                                                                                                         >  8:66691/1‑149 (MQ=255)
                                                                                                                                 ttCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCAGGCCgg                                                                                                                                                                                                                                                                         >  8:1402862/1‑149 (MQ=255)
                                                                                                                                  tCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGt                                                                                                                                                                                                                                                                        >  7:32011/1‑149 (MQ=255)
                                                                                                                                    cGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTAt                                                                                                                                                                                                                                                                      >  5:802129/1‑149 (MQ=255)
                                                                                                                                     gCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTAtt                                                                                                                                                                                                                                                                     <  7:1203963/149‑1 (MQ=255)
                                                                                                                                       aGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTa                                                                                                                                                                                                                                                                   <  6:936048/149‑1 (MQ=255)
                                                                                                                                       aGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTa                                                                                                                                                                                                                                                                   <  8:855733/149‑1 (MQ=255)
                                                                                                                                          ccGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATgg                                                                                                                                                                                                                                                                <  8:518558/149‑1 (MQ=255)
                                                                                                                                            gTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTg                                                                                                                                                                                                                                                              <  6:10478/149‑1 (MQ=255)
                                                                                                                                             tCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGa                                                                                                                                                                                                                                                             >  7:492996/1‑149 (MQ=255)
                                                                                                                                              ccagACGTGCCGGTGGCCGCGTGCCTGGCAGCCATGAACGGCGTAGAACTCGGTGCCCTGCTTCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAGCGTGTTTTCGCTCCCGGCCTGCCGGTATTTATGGTGaa                                                                                                                                                                                                                                                            <  6:1445336/146‑1 (MQ=255)
                                                                                                                                              ccTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGaa                                                                                                                                                                                                                                                            <  6:247389/149‑1 (MQ=255)
                                                                                                                                                tGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGaaca                                                                                                                                                                                                                                                          >  7:1422286/1‑149 (MQ=255)
                                                                                                                                                    gTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGaacaccaa                                                                                                                                                                                                                                                      >  6:1242261/1‑149 (MQ=255)
                                                                                                                                                        tggtggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGaacaccaacacc                                                                                                                                                                                                                                                  >  7:468418/1‑149 (MQ=255)
                                                                                                                                                         ggtggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGATCGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCt                                                                                                                                                                                                                                                 <  8:1287670/149‑1 (MQ=255)
                                                                                                                                                         ggtggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCt                                                                                                                                                                                                                                                 <  8:492996/149‑1 (MQ=255)
                                                                                                                                                         ggtggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCt                                                                                                                                                                                                                                                 <  5:95062/149‑1 (MQ=255)
                                                                                                                                                               cGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGa                                                                                                                                                                                                                                           >  6:1130387/1‑149 (MQ=255)
                                                                                                                                                                gCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGAc                                                                                                                                                                                                                                          >  5:1007443/1‑149 (MQ=255)
                                                                                                                                                                 cTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAACGTTCAGGAATACGTTTCTAACTACATCAAAGCTGACTGGCACGACTCTTCGACCGCCATTTCt                                                                                                           >  2:96745/1‑275 (MQ=255)
                                                                                                                                                                      cTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACctctct                                                                                                                                                                                                                                    >  5:1414840/1‑149 (MQ=255)
                                                                                                                                                                      cTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAgcg                                                                                                      <  2:270679/275‑1 (MQ=255)
                                                                                                                                                                        ggCAGCCATGAACGGCGTAGAGATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGa                                                                                                                                                                                                                                  <  8:7126/149‑1 (MQ=255)
                                                                                                                                                                            gCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCt                                                                                                                                                                                                                              <  8:396217/149‑1 (MQ=255)
                                                                                                                                                                                tGAACGGCTGAGAAATCGGTGCCCTGCTGCTTACTGGCTGTTACGAAATCGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt                                                                                            <  1:37670/275‑1 (MQ=255)
                                                                                                                                                                                 gAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCaga                                                                                                                                                                                                                         >  8:1377276/1‑149 (MQ=255)
                                                                                                                                                                                    cGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCt                                                                                                                                                                                                                      <  7:650658/149‑1 (MQ=255)
                                                                                                                                                                                      gCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTc                                                                                                                                                                                                                    <  5:1130387/149‑1 (MQ=255)
                                                                                                                                                                                        gTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCaa                                                                                                                                                                                                                  >  8:259699/1‑149 (MQ=255)
                                                                                                                                                                                        gTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCaa                                                                                                                                                                                                                  >  6:1363930/1‑149 (MQ=255)
                                                                                                                                                                                          aGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAAcc                                                                                                                                                                                                                >  6:633277/1‑149 (MQ=255)
                                                                                                                                                                                               tCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCACCCTGGaa                                                                                                                                                                                                           <  8:736698/149‑1 (MQ=255)
                                                                                                                                                                                               tCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGaa                                                                                                                                                                                                           <  6:30177/149‑1 (MQ=255)
                                                                                                                                                                                               tCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGaa                                                                                                                                                                                                           <  5:633277/149‑1 (MQ=255)
                                                                                                                                                                                               tCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGaa                                                                                                                                                                                                           <  8:949111/149‑1 (MQ=255)
                                                                                                                                                                                                   tGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTc                                                                                                                                                                                                       <  7:152116/149‑1 (MQ=255)
                                                                                                                                                                                                     cccTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCg                                                                                                                                                                                                     <  8:1194705/149‑1 (MQ=255)
                                                                                                                                                                                                     cccTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCg                                                                                                                                                                                                     >  7:965329/1‑149 (MQ=255)
                                                                                                                                                                                                       ctgctgctgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTcc                                                                                                                                                                                                      >  6:438811/1‑146 (MQ=255)
                                                                                                                                                                                                       ctgctgctgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGt                                                                                                                                                                                                   >  8:302159/1‑149 (MQ=255)
                                                                                                                                                                                                        tgctgctgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGtt                                                                                                                                                                                                  >  8:121908/1‑149 (MQ=255)
                                                                                                                                                                                                            gctgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGa                                                                                                                                                                                                                                  >  5:1238035/1‑113 (MQ=255)
                                                                                                                                                                                                              tgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTTTGAGCCTGCAGAGCTACAACCTGGAAGTTCCGGTTGACGAt                                                                                                                                                                                            <  7:951514/149‑1 (MQ=255)
                                                                                                                                                                                                               gACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATc                                                                                                                                                                                           <  7:1000966/149‑1 (MQ=255)
                                                                                                                                                                                                                aCTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACGCCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCAGTTGACGATCa                                                                                                                                                                                          <  7:828796/149‑1 (MQ=255)
                                                                                                                                                                                                                    gCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGaa                                                                                                                                                                                      <  8:965329/149‑1 (MQ=255)
                                                                                                                                                                                                                    gCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGaa                                                                                                                                                                                      >  6:1349657/1‑149 (MQ=255)
                                                                                                                                                                                                                       gTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGt                                                                                                                                                                                   >  5:170978/1‑149 (MQ=255)
                                                                                                                                                                                                                        ttACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTg                                                                                                                                                                                                              >  6:35664/1‑121 (MQ=255)
                                                                                                                                                                                                                        ttACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTg                                                                                                                                                                                                              <  5:35664/121‑1 (MQ=255)
                                                                                                                                                                                                                          aCGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGt                                                                                                                                                                                                   >  5:748823/1‑130 (MQ=255)
                                                                                                                                                                                                                          aCGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATc                                                                                                                                                                                <  7:922223/149‑1 (MQ=255)
                                                                                                                                                                                                                               aTGGACGCGCGCATTTTTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGaa                                                                                                                                                                           >  7:1292846/1‑149 (MQ=255)
                                                                                                                                                                                                                                tGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGaaa                                                                                                                                                                          <  6:72108/149‑1 (MQ=255)
                                                                                                                                                                                                                                   aCGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGa                                                                                                                                                                                                >  8:224442/1‑124 (MQ=255)
                                                                                                                                                                                                                                   aCGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGa                                                                                                                                                                                                <  7:224442/124‑1 (MQ=255)
                                                                                                                                                                                                                                      cgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAg                                                                                                                                                                    <  8:148157/149‑1 (MQ=255)
                                                                                                                                                                                                                                               cTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGtt                                                                                                                                                           <  8:1002865/149‑1 (MQ=255)
                                                                                                                                                                                                                                               cTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGtt                                                                                                                                                           <  5:774361/149‑1 (MQ=255)
                                                                                                                                                                                                                                                tAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTg                                                                                                                                                          >  7:1443197/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 aaaCTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGaaa                                                                                                                                                                          >  7:1353630/1‑132 (MQ=255)
                                                                                                                                                                                                                                                 aaaCTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGaaa                                                                                                                                                                          <  8:1353630/132‑1 (MQ=255)
                                                                                                                                                                                                                                                       tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt                                                                                                                                                                       >  5:1037379/1‑129 (MQ=255)
                                                                                                                                                                                                                                                       tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt                                                                                                                                                                       <  6:1037379/129‑1 (MQ=255)
                                                                                                                                                                                                                                                       tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTa                                                                                                                                                   >  6:202910/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACAAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc                                                                                                                                                         >  4:233175/1‑143 (MQ=255)
                                                                                                                                                                                                                                                        gCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTAc                                                                                                                                                  >  8:1431771/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           aaCGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAAc                                                                                                                                                     <  2:233175/143‑1 (MQ=255)
                                                                                                                                                                                                                                                            aCGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCa                                                                                                                                              >  5:298794/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  cTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAAc                                                                                                                                            <  6:769161/146‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt                                                                                                                                         <  7:497986/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tttCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTg                                                                                                                                        >  6:270299/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    ttCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACCCCAACGCTGa                                                                                                                                       <  6:1069197/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ttCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc                                                                                                                     <  2:381033/167‑1 (MQ=255)
                                                                                                                                                                                                                                                                     taaatACCGGCAGGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGAc                                                                                                                                      >  8:797322/5‑149 (MQ=255)
                                                                                                                                                                                                                                                                      aaatACCGGCAGGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCACCCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCa                                                                                                                                                                     <  7:1047876/114‑1 (MQ=255)
                                                                                                                                                                                                                                                                        atACCGGCAGGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTgg                                                                                                                                   >  7:528622/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                        atACCGGCAGGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTgg                                                                                                                                   >  5:1472988/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                         tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGGGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACTTCAACGCTGACTgca                                                                                                                                  >  6:430609/1‑147 (MQ=255)
                                                                                                                                                                                                                                                                         tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGATCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa                                                                                                                                  <  7:78660/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa                                                                                                                                  >  5:444115/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa                                                                                                                                  >  8:820711/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                          aCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt                                                                                                                                 <  6:1302181/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCACTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt                                                                                                                                 <  7:119311/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ggCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa                                                                                                                              >  5:405279/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              gCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt                                                                                                                                 <  6:556380/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa                                                                                                                             <  5:519019/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa                                                                                                                             <  7:1316217/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
TCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAACTTGCGCGCAAAGCGGGCAAACGTATCGTACTGCCGGAAGGTGACGAACCGCGT  >  NC_000913/2415454‑2415994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: