Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,581,762 G→A 5.3% A339V (GCC→GTC)  ypfG ← DUF1176 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,581,7620GA 5.3% 591.1 / 13.8 184A339V (GCC→GTC) ypfGDUF1176 family protein
Reads supporting (aligned to +/- strand):  ref base G (90/82);  new base A (6/4);  total (96/88)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 188 total aligned reads displayed.

ATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAA  >  NC_000913/2581497‑2582032
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                              
  gTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGCATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGCATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTATTATCTGGCCTACTTGTCCCGCCATTTTGTTTAACGGGGGCTCCACCACGTGGGCAAGGctgccggcac                                                                                                                                                                                                                                                                      >  4:128569/1‑265 (MQ=255)
         tGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATTTCCCGCCATTTTGTTTAACGGGTGCTCCACAACTTGGGCCAGGCTTCTGGCCCATGccct                                                                                                                                                                                                                                                               >  2:191573/1‑272 (MQ=255)
              ggggCAATTGTCGGAATGACGGGTGACGGGGAAGCTGCTCAGGAGGATAAGCTGTTCCCGTTCTTTGGGTTTACCGCCGAGAATGGTGTGGCAAAAGCGAATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                                                                                                                                                                                                           <  2:243208/273‑1 (MQ=255)
                                       aCGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATTCCAGTTATCGACGGTGGGTTCGGTGGAGTAACGCa                                                                                                                                                                                                                                >  1:16085/1‑275 (MQ=255)
                                                                                                     gtgtgaaaaGGGCATAAGGTGCGTGTAGTGAAAGGTGTCTGTGGGTGATGTCGGGATGCGGGTTGCCGGTGGGGATGCTGAAGCGGCTTCTCCGGCTTACAGCGTCTTATGCGGCCTACAGGTCCCTCCAGTTTGTTTAACGGGTGATCCACAAGGTGGGCCAGGCGTCTGGCACATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGAACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcg                                                                                                                                                                  <  4:41725/270‑1 (MQ=255)
                                                                                                       ggCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCATGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGATTCTGCGGCGTAATGTACCAGGCGAAAACGTCGACCGGCAACGCGCCAGCGCCCCCGAAACCCCCAAtgccctacaccgc                                                                                                                                                                 >  4:104884/1‑262 (MQ=255)
                                                                                                        gCAAAAGGGACACAAGGTGATGGAGGGGAAAGGTGCCTGATTGGGAAGGCCGGTTGTTGATTTCTGGATGGAACGCTGACGCGTCTTACCCGTCCTACGGCGTCTTTTCCGGCCTACTGGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATAGCTTAATCcgcg                                                                                                                                                                <  4:258216/275‑1 (MQ=255)
                                                                                                                        gTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCg                                                                                                                                                                                                                                                                              >  7:802749/1‑149 (MQ=255)
                                                                                                                           cTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCt                                                                                                                                                                                                                                                                           <  6:109225/149‑1 (MQ=255)
                                                                                                                              ggAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGc                                                                                                                                                                                                                                                                        <  8:616060/149‑1 (MQ=255)
                                                                                                                               gAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGcc                                                                                                                                                                                                                                                                       <  5:690367/149‑1 (MQ=255)
                                                                                                                                   gAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATg                                                                                                                                                                                                                                                                   <  7:1091826/149‑1 (MQ=255)
                                                                                                                                        gTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGt                                                                                                                                                                                                                                                              >  8:1347008/1‑149 (MQ=255)
                                                                                                                                        gTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACATGGGCCAGACGCCTGGCCCacgttgtg                                                                                                                                                                                                                                                              <  6:241360/149‑8 (MQ=39)
                                                                                                                                          gCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGNCCAGGCGTCTGGCCCATGCCAGTNAACGNAGGTGGGTTCTGCGGAGTAACGCACCAGGCGAAAACGCTGAACGTCAAAggcc                                                                                                                                                                                                   >  4:347520/1‑203 (MQ=255)
                                                                                                                                          gCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCAACGCTGACGCTTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGAGCCCTTTCGCTAAGGTCACCAGTTCACGCGAttt                                                                                                                               <  2:128802/274‑1 (MQ=255)
                                                                                                                                           ccTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                                                                                                                                                                                                           <  6:1080758/149‑1 (MQ=255)
                                                                                                                                           ccTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                                                                                                                                                                                                           <  6:592568/149‑1 (MQ=255)
                                                                                                                                            cTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCACCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCCCGCAATTtctc                                                                                                                            >  2:219454/1‑275 (MQ=255)
                                                                                                                                               aTGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCAc                                                                                                                                                                                                                               <  2:362216/173‑1 (MQ=255)
                                                                                                                                                tGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAg                                                                                                                                                                                                                                                      <  5:1377625/149‑1 (MQ=255)
                                                                                                                                                   tgattgccggatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGt                                                                                                                                                                                                                                                    <  6:433548/148‑1 (MQ=255)
                                                                                                                                                   tgattgccggatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCAGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTg                                                                                                                                                                                                                                                   >  8:865454/1‑149 (MQ=255)
                                                                                                                                                    gattgccggatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTgg                                                                                                                                                                                                                                                  >  8:1119683/1‑149 (MQ=255)
                                                                                                                                                     attgccggatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTggg                                                                                                                                                                                                                                                 >  6:1260537/1‑149 (MQ=255)
                                                                                                                                                           ggatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgc                                                                                                                                                                                                                                           <  5:375190/149‑1 (MQ=255)
                                                                                                                                                            gatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcg                                                                                                                                                                                                                                          <  7:694782/149‑1 (MQ=255)
                                                                                                                                                            gatgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcg                                                                                                                                                                                                                                          <  8:1139359/149‑1 (MQ=255)
                                                                                                                                                              tgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGtt                                                                                                                                                                                                                                               >  8:727573/1‑142 (MQ=255)
                                                                                                                                                              tgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGtt                                                                                                                                                                                                                                               <  7:727573/142‑1 (MQ=255)
                                                                                                                                                              tgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcggc                                                                                                                                                                                                                                        <  7:306980/149‑1 (MQ=255)
                                                                                                                                                                 tgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTa                                                                                                                                                                                                                                     >  5:547954/1‑149 (MQ=255)
                                                                                                                                                                   attgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAAc                                                                                                                                                                                                                                   >  6:389838/1‑149 (MQ=255)
                                                                                                                                                                    ttgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACg                                                                                                                                                                                                                                  >  5:1071534/1‑149 (MQ=255)
                                                                                                                                                                          gatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAg                                                                                                                                                                                                                            >  6:180026/1‑149 (MQ=255)
                                                                                                                                                                          gatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGACAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAg                                                                                                                                                                                                                            <  8:135812/149‑1 (MQ=255)
                                                                                                                                                                               gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaa                                                                                                                                                                                                                       >  8:544839/1‑149 (MQ=255)
                                                                                                                                                                               gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaa                                                                                                                                                                                                                       >  5:440313/1‑149 (MQ=255)
                                                                                                                                                                                 cGCTGACGCGTCTTATCCGGCCTACACCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCAACAACGTGGGCCAGGCGTCTAGCCCATGCCAGTTATCGCAGGTGGTTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                                                                                                                                                     <  6:694303/149‑1 (MQ=255)
                                                                                                                                                                                  gCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAAc                                                                                                                                                                                                                                   <  6:1347754/134‑1 (MQ=255)
                                                                                                                                                                                  gCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAAc                                                                                                                                                                                                                                   >  5:1347754/1‑134 (MQ=255)
                                                                                                                                                                                   atgctgcgTCTTATCCTGCCTACAGCGTCTTATCCGGCCTACATGGCCCGCCATTTTGGTTACCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGtctc                                                                                     <  2:182957/270‑1 (MQ=255)
                                                                                                                                                                                       cgcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGa                                                                                                                                                                                                               <  8:878006/149‑1 (MQ=255)
                                                                                                                                                                                       cgcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGa                                                                                                                                                                                                               <  6:335734/149‑1 (MQ=255)
                                                                                                                                                                                        gcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                                                                                                                                              >  6:1008990/1‑149 (MQ=255)
                                                                                                                                                                                        gcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                                                                                                                                              >  6:62214/1‑149 (MQ=255)
                                                                                                                                                                                          gTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCg                                                                                                                                                                                                            >  7:1324847/1‑149 (MQ=255)
                                                                                                                                                                                           tCTTATCCGGCCTACAGCGTCTTATCTGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGt                                                                                                                                                                                                           >  8:1247367/1‑149 (MQ=255)
                                                                                                                                                                                           tCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGt                                                                                                                                                                                                           >  5:1040233/1‑149 (MQ=255)
                                                                                                                                                                                           tCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCttt                                                                                                                                                          >  2:81565/1‑198 (MQ=255)
                                                                                                                                                                                            cTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTc                                                                                                                                                                                                          <  5:180026/149‑1 (MQ=255)
                                                                                                                                                                                            cTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTc                                                                                                                                                                                                          <  7:995752/149‑1 (MQ=255)
                                                                                                                                                                                               aTCCGGCCTACGGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCt                                                                                                                                                      <  4:81565/198‑1 (MQ=255)
                                                                                                                                                                                               aTCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCaaa                                                                                                                                                                                                       <  8:1013655/149‑1 (MQ=255)
                                                                                                                                                                                                tCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAg                                                                                                                                                                                                      >  7:702837/1‑149 (MQ=255)
                                                                                                                                                                                                 ccGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgc                                                                                                                                                                                                     <  8:674799/149‑1 (MQ=255)
                                                                                                                                                                                                   ggCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgcgc                                                                                                                                                                                                   <  6:27045/149‑1 (MQ=255)
                                                                                                                                                                                                             gTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCt                                                                                                                                                                                         <  5:1309280/149‑1 (MQ=255)
                                                                                                                                                                                                             gTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCt                                                                                                                                                                                         <  5:867106/149‑1 (MQ=255)
                                                                                                                                                                                                              tCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAg                                                                                                                                                                                                                                                               >  2:124204/1‑78 (MQ=255)
                                                                                                                                                                                                              tCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg                                                                                                                                                                                        >  7:187283/1‑149 (MQ=255)
                                                                                                                                                                                                                     cGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGcc                                                                                                                                                                                                  <  7:73773/132‑1 (MQ=255)
                                                                                                                                                                                                                           aCATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCg                                                                                                                                                                           >  7:136708/1‑149 (MQ=255)
                                                                                                                                                                                                                            cATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                                                                                                                                          >  5:940506/1‑149 (MQ=255)
                                                                                                                                                                                                                            cATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                                                                                                                                          <  5:1260537/149‑1 (MQ=255)
                                                                                                                                                                                                                             aTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCt                                                                                                                                                                         <  7:914844/149‑1 (MQ=255)
                                                                                                                                                                                                                                 cccGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAg                                                                                                                                           >  2:286532/1‑175 (MQ=255)
                                                                                                                                                                                                                                   cGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                                                                                                                                                   >  6:985731/1‑149 (MQ=255)
                                                                                                                                                                                                                                    gCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcg                                                                                                                                                                  >  7:721219/1‑149 (MQ=255)
                                                                                                                                                                                                                                     ccATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCa                                                                                                                                       <  4:286532/175‑1 (MQ=255)
                                                                                                                                                                                                                                        ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGcc                                                                                                                                                              >  6:703831/1‑149 (MQ=255)
                                                                                                                                                                                                                                        ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGcc                                                                                                                                                              >  8:1399522/1‑149 (MQ=255)
                                                                                                                                                                                                                                        ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGcc                                                                                                                                                              >  6:1305832/1‑149 (MQ=255)
                                                                                                                                                                                                                                          ttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCt                                                                                                                                                            <  6:940506/149‑1 (MQ=255)
                                                                                                                                                                                                                                             tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                                                                                                                                              >  6:476980/1‑96 (MQ=255)
                                                                                                                                                                                                                                             tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                                                                                                                                                         >  5:758321/1‑149 (MQ=255)
                                                                                                                                                                                                                                               tAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGc                                                                                                                                                       <  7:544839/149‑1 (MQ=255)
                                                                                                                                                                                                                                                aaCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCt                                                                                                                                                      >  7:870618/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  cGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTaa                                                                                                                                                    <  6:1040233/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                                                                                                                                                   >  8:282026/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGAGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                                                                                                                                                   >  6:895327/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       gATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGc                                                                                                                                                                                   >  2:278380/1‑113 (MQ=255)
                                                                                                                                                                                                                                                         tCCAAAACGTGTGCCATGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTCCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCACCCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATc               <  1:37186/275‑1 (MQ=255)
                                                                                                                                                                                                                                                          ccACAGCGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCt                                                                                                                                                                                         <  6:84036/104‑1 (MQ=255)
                                                                                                                                                                                                                                                           cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCcaca                                                                                                                                                                               <  4:278380/113‑1 (MQ=255)
                                                                                                                                                                                                                                                           cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAg                                                                                                                                           >  7:1156749/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGt                                                                                                                                          >  5:635943/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              aaCGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTc                                                                                                                                        <  7:1347008/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                cGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAc                                                                                                                                      <  8:155606/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg                                                                                                                                     >  8:455963/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg                                                                                                                                     >  7:1424399/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                 atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg                                                                                                                                     >  5:722582/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                  tGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                                                                                                                                                   <  6:358197/134‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                                                                                                                                                   >  5:358197/1‑134 (MQ=255)
                                                                                                                                                                                                                                                                  tGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcgc                                                                                                                                    >  5:1141566/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    ggCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttccagtt                                                                                               >  4:190217/1‑184 (MQ=255)
                                                                                                                                                                                                                                                                       cAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCttt                                                                                                                                                          >  8:434253/1‑122 (MQ=255)
                                                                                                                                                                                                                                                                       cAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCttt                                                                                                                                                          <  7:434253/122‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cAGACGCCTGGCCCACGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCTCCTGAATGc                                                                                                                                                                                   <  8:938876/97‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cAGACGCCTGGCCCACGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGc                                                                                                                                                                                   >  7:938876/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                              
ATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAA  >  NC_000913/2581497‑2582032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: