Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,691,171 T→A 5.5% noncoding (170/184 nt) glmY ← sRNA activator of glmS mRNA, glmZ processing antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,1710TA 5.5% 813.0 / 25.4 231noncoding (170/184 nt)glmYsRNA activator of glmS mRNA, glmZ processing antagonist
Reads supporting (aligned to +/- strand):  ref base T (102/115);  new base A (9/4);  total (111/120)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 232 total aligned reads displayed.

TCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGTTACTATTATGCAGTAACTGTGCCAATAAAGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTC  >  NC_000913/2690897‑2691429
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                  
     aGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTCACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTAcca                                                                                                                                                                                                                                                               >  2:161231/1‑274 (MQ=255)
                                               aGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTAAGATACAACAACGCCGGGAGTTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTca                                                                                                                                                                                                                     >  2:153876/1‑274 (MQ=255)
                                                                        ccTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGAAATTACCCGGATTTGTTATGGAAGAAGGCGGTGCCTAACTCGACGATTCACCCGAAGGGTGATATAGGTaagatg                                                                                                                                                                                            >  1:29182/1‑270 (MQ=255)
                                                                                       cATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAACTACGCTGATATCAGAAGTTGGa                                                                                                                                                                             >  2:219452/1‑275 (MQ=255)
                                                                                                  tGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCCGATATCAGAAGTTGGACGGCAGGCAcc                                                                                                                                                                  >  2:137391/1‑275 (MQ=255)
                                                                                                                        ccAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATGACCGGTTGTCGGAATGGGCCGGGAAAAACGGGCCAGCGTTTCAAGGGGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAtt                                                                                                                                             <  4:219452/274‑1 (MQ=255)
                                                                                                                              ggggCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCt                                                                                                                                                                                                                                                           <  4:317386/158‑1 (MQ=255)
                                                                                                                                    gcagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCg                                                                                                                                                                                                                                                              >  7:1346482/1‑149 (MQ=255)
                                                                                                                                     cagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGTCGGCCGGCACCTTTTTGTGCGTCATTCGTAtt                                                                                                                                             >  4:58962/1‑261 (MQ=255)
                                                                                                                                       gAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCt                                                                                                                                                                                                                                                           >  8:926621/1‑149 (MQ=255)
                                                                                                                                        aaTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCtt                                                                                                                                                                                                                                                          <  6:231599/149‑1 (MQ=255)
                                                                                                                                         aTCAGCAAAAAGGCCAGCATTACCAGTGTTCGTAAGGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGGGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTAt                                                                                                                                         <  2:58962/261‑1 (MQ=255)
                                                                                                                                           cAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTGt                                                                                                                                                                                                                                                       <  8:669592/149‑1 (MQ=255)
                                                                                                                                              cAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCCCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTAt                                                                                                                                                                                                                                                    <  8:690540/149‑1 (MQ=255)
                                                                                                                                               aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATg                                                                                                                                                                                                                                                   >  5:295348/1‑149 (MQ=255)
                                                                                                                                               aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATg                                                                                                                                                                                                                                                   >  7:366215/1‑149 (MQ=255)
                                                                                                                                                  aaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTGttgtatct                                                                                                                                                                                                                                                <  8:570945/149‑7 (MQ=39)
                                                                                                                                                       cAGCATTACCAGTTGACGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTAc                                                                                                                                                                                                                                                                 <  7:611825/127‑1 (MQ=255)
                                                                                                                                                        aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGc                                                                                                                                                                                                                                          >  5:777813/1‑149 (MQ=255)
                                                                                                                                                        aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGc                                                                                                                                                                                                                                          >  6:897997/1‑149 (MQ=255)
                                                                                                                                                           aTTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCAGt                                                                                                                                                                                                                                       <  7:722649/149‑1 (MQ=255)
                                                                                                                                                             tatcAGTTGGTGGAATGAGCGGGGAAAAACGGGCCAGCGTTGCAAGGGGGTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCAAGGGGAATGGCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCg                                                                                                         <  4:153876/270‑1 (MQ=255)
                                                                                                                                                             tACCGGTTGGCGGGATGGGCGGGAAAAAACGGGCCAGCTGTTCAAGGTGTTACTCGCGTCAGACGCGAAGAGCATGATGCTAACCGAGGGGAAGTGCAGATGCAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTGCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGt                                                                                                       <  4:137391/275‑1 (MQ=255)
                                                                                                                                                               ccAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGGTATGGAATAAGGCGGTGCCTAACTCGGCGTTTCGCCCGATGGTTGATATATCTACGCTGATATCAGAAGTTGGACGGGAGGCACCTTGTTGGGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGCCATAAGTCGATgt                                                                                                     >  2:301467/1‑274 (MQ=255)
                                                                                                                                                                 agatGTCGTAATGACCGGGGAAAAACGGGCTAGCATTTCAAGGTGTTACTCTCGTCAGACGTGAATAGCCTGATGCTAACCGAGGGGAAGGTCAGATACCACAAAACCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTaa                                                                                                                                                                                                                                 <  8:899578/146‑1 (MQ=255)
                                                                                                                                                                 aGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTaa                                                                                                                                                                                                                                 >  7:424587/1‑149 (MQ=255)
                                                                                                                                                                 aGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTaa                                                                                                                                                                                                                                 >  8:154420/1‑149 (MQ=255)
                                                                                                                                                                  gTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAc                                                                                                                                                                                                                                >  6:964140/1‑149 (MQ=255)
                                                                                                                                                                      tCGTAATGAGCGGGGAAAATCGGGGCAGCGTGTCAAGGTGTTACTCTCGTCAGTCGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGa                                                                                                                                                                                                                            <  5:44729/149‑1 (MQ=255)
                                                                                                                                                                       cGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAc                                                                                                                                                                                                                           <  6:207882/149‑1 (MQ=255)
                                                                                                                                                                       cGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAc                                                                                                                                                                                                                           <  7:1318165/149‑1 (MQ=255)
                                                                                                                                                                         tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAc                                                                                                                                                                                                                                >  5:311326/1‑142 (MQ=255)
                                                                                                                                                                            tgggCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGGGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGt                                                                                        <  2:53589/272‑1 (MQ=255)
                                                                                                                                                                            tggagggggAAAAACGAGCCAGCGTTTCAAGGGGTTACTACCCGTAGGCGAGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGGGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGGGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGt                                                                                        <  2:250816/270‑1 (MQ=255)
                                                                                                                                                                            tGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTc                                                                                                                                                                                                                      <  8:1013065/149‑1 (MQ=255)
                                                                                                                                                                            ggggCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCCCGGCAGACGCTAATAGCCTGAGGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGTTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGt                                                                                        <  4:161231/272‑1 (MQ=255)
                                                                                                                                                                                cGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGccc                                                                                                                                                                                                                  <  6:295348/149‑1 (MQ=255)
                                                                                                                                                                                  ggggAAGACGGGCCACAGTTTAAAGTTGTTACGCTCTAAAGGCGTACATGGCGTATTGGTAACGTAGGGGAATGTCAGATACATCAAAGCCGGGAATGACACGGCTTTGTTATGGAATAAGACGGTGACTAACTCGACGTTTCGCAAGATGGATGATATCGCTACGCTGATATCAGAAGTTGGACGGCAGGAACCTTGTTGTGCGTCATTCGTATTTTATGTAGCAAGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGTTAc                                                                                     <  4:277399/272‑1 (MQ=255)
                                                                                                                                                                                   ggAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGAt                                                                                                                                                                                                               <  7:926621/149‑1 (MQ=255)
                                                                                                                                                                                    gAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATg                                                                                                                                                                                                              >  5:1031121/1‑149 (MQ=255)
                                                                                                                                                                                    gAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATg                                                                                                                                                                                                              <  7:159801/149‑1 (MQ=255)
                                                                                                                                                                                       aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAg                                                                                                                                                                                                                                            >  5:846071/1‑116 (MQ=255)
                                                                                                                                                                                       aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAg                                                                                                                                                                                                                                            <  6:846071/116‑1 (MQ=255)
                                                                                                                                                                                        aaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTg                                                                                                                                                                                                          <  5:112478/149‑1 (MQ=255)
                                                                                                                                                                                        aaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAATCGACGTTTCGCCCGATGGTTg                                                                                                                                                                                                          >  7:826452/1‑149 (MQ=255)
                                                                                                                                                                                             gCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCttt                                                                                                                                                                                                                                                         <  5:627283/97‑1 (MQ=39)
                                                                                                                                                                                             gCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCttt                                                                                                                                                                                                                                                         >  6:627283/1‑97 (MQ=39)
                                                                                                                                                                                               cAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGc                                                                                                                                                                                                   <  5:413262/149‑1 (MQ=255)
                                                                                                                                                                                                aGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                                                                                                                                           <  8:310243/140‑1 (MQ=255)
                                                                                                                                                                                                aGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCt                                                                                                                                                                                                  >  7:876399/1‑149 (MQ=255)
                                                                                                                                                                                                           gtgtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATc                                                                                                                                                                                       >  8:724750/1‑149 (MQ=255)
                                                                                                                                                                                                            tgtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGAtata                                                                                                                                                                                                     <  7:856743/134‑1 (MQ=255)
                                                                                                                                                                                                            tgtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCa                                                                                                                                                                                      <  8:5966/149‑1 (MQ=255)
                                                                                                                                                                                                             gtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGttt                                                                                                                                                                                                                       <  7:1128404/115‑1 (MQ=255)
                                                                                                                                                                                                             gtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGttt                                                                                                                                                                                                                       >  8:1128404/1‑115 (MQ=255)
                                                                                                                                                                                                             gtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTg                                                                                                                   >  2:71639/1‑215 (MQ=255)
                                                                                                                                                                                                                 ctctCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGt                                                                                                                                                                                 <  7:1280696/149‑1 (MQ=255)
                                                                                                                                                                                                                 ctctCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGt                                                                                                                                                                                 >  8:435891/1‑149 (MQ=255)
                                                                                                                                                                                                                 ctctCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACAt                                                                                                               <  4:71639/215‑1 (MQ=255)
                                                                                                                                                                                                                  tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAc                                                                                                                                                                                                                                <  7:66998/101‑1 (MQ=255)
                                                                                                                                                                                                                  tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                                                                                                                                                >  6:1037705/1‑149 (MQ=255)
                                                                                                                                                                                                                        tCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACg                                                                                                                                                                           >  7:1037258/1‑149 (MQ=255)
                                                                                                                                                                                                                           gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGatat                                                                                                                                                                                        >  5:70233/1‑133 (MQ=255)
                                                                                                                                                                                                                           gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGatat                                                                                                                                                                                        <  6:70233/133‑1 (MQ=255)
                                                                                                                                                                                                                           gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggca                                                                                                                                                                        <  5:906886/149‑1 (MQ=255)
                                                                                                                                                                                                                           gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggca                                                                                                                                                                        <  8:526617/149‑1 (MQ=255)
                                                                                                                                                                                                                            aCGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcag                                                                                                                                                                       >  7:484136/1‑149 (MQ=255)
                                                                                                                                                                                                                                gAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCAc                                                                                                                                                                   >  6:420538/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCtt                                                                                                                                                                <  7:1452442/149‑1 (MQ=255)
                                                                                                                                                                                                                                      ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCg                                                                                                                                                                                                                                         >  6:954659/1‑73 (MQ=255)
                                                                                                                                                                                                                                      ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCg                                                                                                                                                                                                                                         <  5:954659/73‑1 (MQ=255)
                                                                                                                                                                                                                                      ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                                                                                                                                             <  7:266407/149‑1 (MQ=255)
                                                                                                                                                                                                                                        tGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                                                                                                                                           <  8:103301/149‑1 (MQ=255)
                                                                                                                                                                                                                                        tGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                                                                                                                                           >  7:192761/1‑149 (MQ=255)
                                                                                                                                                                                                                                          aTGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGc                                                                                                                                                         >  5:975684/1‑149 (MQ=255)
                                                                                                                                                                                                                                           tGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCg                                                                                                                                                        >  5:1442277/1‑149 (MQ=255)
                                                                                                                                                                                                                                              tAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCa                                                                                                                                                     >  8:1388865/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aCCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCAtt                                                                                                                                                   <  6:683085/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 ccGAGGGGAAGTTCAGATTCAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTc                                                                                                                                                  <  7:219731/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                                                                                                                                           <  7:316390/139‑1 (MQ=255)
                                                                                                                                                                                                                                                   gAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                                                                                                                                           >  6:411614/1‑90 (MQ=255)
                                                                                                                                                                                                                                                   gAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGt                                                                                                                                                >  7:282292/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     ggggAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAt                                                                                                                                              <  8:166688/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       ggAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACGCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAttt                                                                                                                                            <  8:826452/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           gTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                                                                                                                                            >  7:448998/1‑113 (MQ=255)
                                                                                                                                                                                                                                                              cAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAg                                                                                                                                     <  8:1346482/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGc                                                                                                                                    <  6:1288305/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ataacaaAGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGt                                                                                                                                >  5:1309924/3‑149 (MQ=255)
                                                                                                                                                                                                                                                                   ataacaaAGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGt                                                                                                                                >  5:1283002/3‑149 (MQ=255)
                                                                                                                                                                                                                                                                     aacaaAGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGATACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc                                                                                                                              >  8:25016/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     aacaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc                                                                                                                              >  8:1280666/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      acaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc                                                                                                                             >  6:760118/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        aaaGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGa                                                                                                                           >  7:346498/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        aaaGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGa                                                                                                                           >  5:877049/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         aaGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa                                                                                                                          >  8:355396/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         aaGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa                                                                                                                          <  8:192761/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAg                                                                                                                         <  8:393508/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAg                                                                                                                         <  8:280680/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACgg                                                                                                                                                                          >  5:484032/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                             cGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACgg                                                                                                                                                                          <  6:484032/97‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                  
TCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATAAGTCGGTGAATGAGCCACTGGTTACTATTATGCAGTAACTGTGCCAATAAAGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTC  >  NC_000913/2690897‑2691429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: