Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,304 A→G 5.2% intergenic (‑241/+31) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3040AG 5.2% 449.0 / 9.8 135intergenic (‑241/+31)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base A (69/59);  new base G (4/3);  total (73/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

Only 100 of 135 total aligned reads displayed.

TACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATC  >  NC_000913/2704068‑2704574
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                              
tACAAATAAATAAATGCGTCAACAATTCATCACACGAAACAGACCTAATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGGTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGa                                                                                                                                                                                                                                          <  4:29551/275‑1 (MQ=255)
                                               atatACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCAAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGCTGCCATCACGGCGCAAAAGTGAACGAAGATGACTATTAATGGATGCTGCAAATGCGACTCAAGAGCAGACCAGTCGGCAAttacgcttc                                                                                                                                                                                            >  1:4458/1‑267 (MQ=255)
                                                            tGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGTATACCGCCAATGCGACTTAAGCGCAGACCAATCGGCCATTGCCCTTCTTTCTTATCGAAGc                                                                                                                                                                              >  2:263340/1‑275 (MQ=255)
                                                              cTAACTGCTTCGCTGCAGTTTGAGGTTACCGGTATATGGGGACCAACGCTGGTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAAAGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTc                                                                                                                                                                            <  2:330821/275‑1 (MQ=255)
                                                                                           cGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATaa                                                                                                                                                                                                                                                                             >  8:886045/1‑149 (MQ=255)
                                                                                           cGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATaa                                                                                                                                                                                                                                                                             >  8:1046829/1‑149 (MQ=255)
                                                                                               atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTACCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                                                                                                                                         <  7:345905/149‑1 (MQ=255)
                                                                                                   ggggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACa                                                                                                                                                                                                                                                                     >  7:499893/1‑149 (MQ=255)
                                                                                                    gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACaa                                                                                                                                                                                                                                                                    <  8:1180564/149‑1 (MQ=255)
                                                                                                    gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACaa                                                                                                                                                                                                                                                                    >  5:179447/1‑149 (MQ=255)
                                                                                                     ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGCCCGACCGGATTCCCTTc                                                                                                                                      >  4:39818/1‑274 (MQ=255)
                                                                                                       aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGCTTTATCTCATCAATAATTCACGTTGTCGCCATCACGGCGACAACGTGAACCAAGCTGCCTATTAATGAATGCATCTAATCCGACTTAAGCGCAGACCAGTCGGCCACTCGCCTTCTTTCCTACCGAAGCTCAACCAGATAGCCGGTACCCGCCGTCCCAGGTTCCCTTCCgc                                                                                                                                   >  4:146954/1‑274 (MQ=255)
                                                                                                       aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTAGGCCATTCGCCTTCTTGCTTATCGAAGCTGATCCAGATAACCGTTGCGCGACCGACCCGATACGCTTACgg                                                                                                                                   >  1:70537/1‑275 (MQ=255)
                                                                                                        ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTg                                                                                                                                                                                                                                                                <  8:491489/149‑1 (MQ=255)
                                                                                                        ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGCCCAGATTCGCTTCCGGc                                                                                                                                  >  2:162447/1‑275 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                                                                                                                                             >  7:457603/1‑149 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                                                                                                                                             <  6:154386/149‑1 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCAACAACTTACACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCCGATAGCCGTTGCCCGACCGACAGGATTATCTTCCGGccca                                                                                                                               >  2:340543/1‑275 (MQ=255)
                                                                                                                gTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGa                                                                                                                                                                                                                                                        <  8:457603/149‑1 (MQ=255)
                                                                                                                  ttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                                                                                                                                                      <  6:1187209/149‑1 (MQ=255)
                                                                                                                    cGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGc                                                                                                                                                                                                                                                    <  6:309988/149‑1 (MQ=255)
                                                                                                                          ccGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                                                                                                                                              <  7:697685/149‑1 (MQ=255)
                                                                                                                          ccGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                                                                                                                                              >  7:316801/1‑149 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                            <  6:996673/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                            <  7:608378/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                            <  7:816162/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                            <  7:967593/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                            >  8:680308/1‑149 (MQ=255)
                                                                                                                               gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                                                                                                                                         >  8:773825/1‑149 (MQ=255)
                                                                                                                               gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                                                                                                                                         <  8:316801/149‑1 (MQ=255)
                                                                                                                                 aaCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                                                                                                                                                       >  7:583827/1‑149 (MQ=255)
                                                                                                                                     tGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgccgcc                                                                                                                                                                                                                                   <  8:1085233/149‑1 (MQ=255)
                                                                                                                                       gAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCaa                                                                                                                                                                                                                                 <  8:1249400/149‑1 (MQ=255)
                                                                                                                                          gTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                                                                                                                                              >  5:57227/1‑149 (MQ=255)
                                                                                                                                          gTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                                                                                                                                              <  7:504016/149‑1 (MQ=255)
                                                                                                                                            gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                                                                                                                                            <  7:200362/149‑1 (MQ=255)
                                                                                                                                            gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                                                                                                                                            <  6:179447/149‑1 (MQ=255)
                                                                                                                                            gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGATCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTc                                                                                               >  2:223165/1‑274 (MQ=255)
                                                                                                                                             ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATAACGGCGACAACGTTAACGAAGATGGCTATTACTGGATGCCGCCAATGCGACTTAAGCGCAGACCCGTCGGCCATTCGCCTTCTTTCCTATCGAACCACATCCCGTTAGCCGTTCCCAGCCCACCCCGCTCCCCTTCCCGCACAACCCCCCCGCCACTGCTACCCGCGCttgttccc                                                                                              >  4:8597/1‑267 (MQ=255)
                                                                                                                                             ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                                                                                                                                                      >  2:82442/1‑122 (MQ=255)
                                                                                                                                                aCCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTa                                                                                                                                                                                                                        >  5:790686/1‑149 (MQ=255)
                                                                                                                                                 ccTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTa                                                                                                                                                                                                                                                  <  4:82442/122‑1 (MQ=255)
                                                                                                                                                  cTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                                                                                                                                                      >  7:545608/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                     >  8:847891/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                     >  5:520742/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACAAAACGACGGGGGATGTCGTTAGCCAAGGAAGATTTATCTCATAAATAATTGACGTTGCCGCAATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                     <  8:830325/149‑1 (MQ=255)
                                                                                                                                                      aaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                                                                                                                                                  >  5:1053621/1‑149 (MQ=255)
                                                                                                                                                       aCTTCGGGTGAATATTCTATACACAACGACGGGTGATGTCGTTAGCCACGGGCGATTTATCGCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGc                                                                                    <  2:288713/274‑1 (MQ=255)
                                                                                                                                                           cGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACCGCTGTCCGGCCCGTTTTCCCGGTTTTCCCCcaac                                                                               >  4:147086/1‑273 (MQ=255)
                                                                                                                                                              gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGt                                                                                                                                                                                                                                                                 <  5:193734/94‑1 (MQ=255)
                                                                                                                                                                gAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAAt                                                                                                                                                                                                                                >  5:955204/1‑125 (MQ=255)
                                                                                                                                                                gAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAAt                                                                                                                                                                                                                                <  6:955204/125‑1 (MQ=255)
                                                                                                                                                                 aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                                                                                                                                                   <  6:323338/105‑1 (MQ=255)
                                                                                                                                                                 aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                                                                                                                                                   >  5:323338/1‑105 (MQ=255)
                                                                                                                                                                   tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCa                                                                                                                                                                                                     <  5:1231829/149‑1 (MQ=255)
                                                                                                                                                                     ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                                   >  5:1115870/1‑149 (MQ=255)
                                                                                                                                                                     ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                                   <  6:83847/149‑1 (MQ=255)
                                                                                                                                                                        tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                                                                                                                                                <  5:245495/149‑1 (MQ=255)
                                                                                                                                                                        tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                                                                                                                                                >  5:780440/1‑149 (MQ=255)
                                                                                                                                                                          taCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCct                                                                                                                                                                                              <  5:1213053/149‑1 (MQ=255)
                                                                                                                                                                               aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                                                                                                                                         >  7:560280/1‑149 (MQ=255)
                                                                                                                                                                                acgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                                                                                                                                                        <  8:499893/149‑1 (MQ=255)
                                                                                                                                                                                        ggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                <  6:1115870/149‑1 (MQ=255)
                                                                                                                                                                                        ggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCcatcat                                                                             <  2:53864/248‑1 (MQ=255)
                                                                                                                                                                                            gTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgac                                                                                                                                                      >  2:120365/1‑171 (MQ=255)
                                                                                                                                                                                              cGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCAt                                                                                                                                                                          <  8:805754/149‑1 (MQ=255)
                                                                                                                                                                                               gTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGCTGGCTATTAATGGATGCCTCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATc                                                                                                                                                                         >  7:893942/1‑149 (MQ=255)
                                                                                                                                                                                                ttAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                                  <  4:120365/171‑1 (MQ=255)
                                                                                                                                                                                                  aGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGACGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTCTTGTCCCGGAGGAAAAATCCCGTTTGCCAg                                         >  2:184524/1‑274 (MQ=255)
                                                                                                                                                                                                   gCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                                                                                                                                                     <  7:769858/149‑1 (MQ=255)
                                                                                                                                                                                                           agaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGtt                                                                                                                                                             <  8:545608/149‑1 (MQ=255)
                                                                                                                                                                                                           agaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGtt                                                                                                                                                             <  6:780440/149‑1 (MQ=255)
                                                                                                                                                                                                             aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGc                                                                                                                                                           <  6:520742/149‑1 (MQ=255)
                                                                                                                                                                                                                tttctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGATCATCCAGATAGCCGTTGCccg                                                                                                                                                        >  5:1357683/3‑149 (MQ=255)
                                                                                                                                                                                                                  tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgac                                                                                                                                                      >  8:605892/1‑149 (MQ=255)
                                                                                                                                                                                                                   ctcATAGATAATTCACGTGGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATCGATGCCGCCAATGCGACTTAAGCGAAGGCCAGTCGGCCATTAACCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGGTTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGgctgct                        <  2:34191/274‑1 (MQ=255)
                                                                                                                                                                                                                    tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                                                                                                                                                    >  5:35590/1‑149 (MQ=255)
                                                                                                                                                                                                                     cATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAg                                                                                                                                                                  <  6:1339269/134‑1 (MQ=255)
                                                                                                                                                                                                                      ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                                  >  8:1278164/1‑149 (MQ=255)
                                                                                                                                                                                                                        aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                                                                                                                                                <  6:365215/149‑1 (MQ=255)
                                                                                                                                                                                                                        aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                                                                                                                                                >  6:746497/1‑149 (MQ=255)
                                                                                                                                                                                                                         aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTc                                                                                                                                                                                                  <  8:552692/98‑1 (MQ=255)
                                                                                                                                                                                                                         aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAg                                                                                                                                               >  7:1329147/1‑149 (MQ=255)
                                                                                                                                                                                                                          ataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGa                                                                                                                                              <  8:1438379/149‑1 (MQ=255)
                                                                                                                                                                                                                             gttCACGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                           >  6:303405/2‑149 (MQ=255)
                                                                                                                                                                                                                             atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCCACTTAAGCCCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAACCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCCTCCGGCACAAAGCCCCAGTCACCGCTGTCCGCGCTGTCGTCCCGGTTGTCGCCCATCATGAAGTATAGTCCCGGTCGAATAATCCAGGTTGCCAGGTGTTGCCCTGGCTGCTGGAAATACAt              >  4:39251/1‑274 (MQ=255)
                                                                                                                                                                                                                              ttCACGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGccc                                                                                                                                                         <  7:690389/134‑1 (MQ=255)
                                                                                                                                                                                                                              ttCACGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGccc                                                                                                                                                         >  8:690389/1‑134 (MQ=255)
                                                                                                                                                                                                                                cACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAGGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCACATCATGAAg                                                                         >  2:96023/1‑212 (MQ=255)
                                                                                                                                                                                                                                 aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCtt                                                                                                                                       >  7:440414/1‑149 (MQ=255)
                                                                                                                                                                                                                                    ttGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg                                                                                                                                    >  5:422053/1‑149 (MQ=255)
                                                                                                                                                                                                                                    ttGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg                                                                                                                                    >  6:546067/1‑149 (MQ=255)
                                                                                                                                                                                                                                       tCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATACCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                           >  8:1342771/1‑139 (MQ=255)
                                                                                                                                                                                                                                       tCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATACCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                           <  7:1342771/139‑1 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTc                                                                                                           >  2:345206/1‑169 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCCGTTGTCGCCGACCATGAAGTATTGTCCCGGAGGAACAATGCAGGTTGCCAGCTTTTGCCCTGGCCGCTGGCCATACCACCCCACCTGacc  >  4:45520/1‑272 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCAGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                >  8:488927/1‑100 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCAGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                <  7:488927/100‑1 (MQ=255)
                                                                                                                                                                                                                                          ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTa                                                                       <  4:254943/204‑1 (MQ=255)
                                                                                                                                                                                                                                           cATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa                                                                                                                             <  6:790686/149‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                              
TACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATC  >  NC_000913/2704068‑2704574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: