Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,308 C→T 5.1% intergenic (‑245/+27) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3080CT 5.1% 450.6 / 10.1 138intergenic (‑245/+27)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base C (68/62);  new base T (4/3);  total (72/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-02

Only 100 of 138 total aligned reads displayed.

TACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCG  >  NC_000913/2704068‑2704579
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
tACAAATAAATAAATGCGTCAACAATTCATCACACGAAACAGACCTAATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGGTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGa                                                                                                                                                                                                                                               <  4:29551/275‑1 (MQ=255)
                                               atatACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCAAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGCTGCCATCACGGCGCAAAAGTGAACGAAGATGACTATTAATGGATGCTGCAAATGCGACTCAAGAGCAGACCAGTCGGCAAttacgcttc                                                                                                                                                                                                 >  1:4458/1‑267 (MQ=255)
                                                            tGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGTATACCGCCAATGCGACTTAAGCGCAGACCAATCGGCCATTGCCCTTCTTTCTTATCGAAGc                                                                                                                                                                                   >  2:263340/1‑275 (MQ=255)
                                                              cTAACTGCTTCGCTGCAGTTTGAGGTTACCGGTATATGGGGACCAACGCTGGTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAAAGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTc                                                                                                                                                                                 <  2:330821/275‑1 (MQ=255)
                                                                                                   ggggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACa                                                                                                                                                                                                                                                                          >  7:499893/1‑149 (MQ=255)
                                                                                                    gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACaa                                                                                                                                                                                                                                                                         <  6:69065/149‑1 (MQ=255)
                                                                                                    gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACaa                                                                                                                                                                                                                                                                         <  8:1180564/149‑1 (MQ=255)
                                                                                                     ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGCCCGACCGGATTCCCTTc                                                                                                                                           >  4:39818/1‑274 (MQ=255)
                                                                                                     ggACCAACGCTGTTTCGTGAGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                                                                                                                                                        <  8:834006/149‑1 (MQ=255)
                                                                                                       aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGCTTTATCTCATCAATAATTCACGTTGTCGCCATCACGGCGACAACGTGAACCAAGCTGCCTATTAATGAATGCATCTAATCCGACTTAAGCGCAGACCAGTCGGCCACTCGCCTTCTTTCCTACCGAAGCTCAACCAGATAGCCGGTACCCGCCGTCCCAGGTTCCCTTCCgc                                                                                                                                        >  4:146954/1‑274 (MQ=255)
                                                                                                       aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTAGGCCATTCGCCTTCTTGCTTATCGAAGCTGATCCAGATAACCGTTGCGCGACCGACCCGATACGCTTACgg                                                                                                                                        >  1:70537/1‑275 (MQ=255)
                                                                                                        ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTg                                                                                                                                                                                                                                                                     <  8:491489/149‑1 (MQ=255)
                                                                                                        ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGCCCAGATTCGCTTCCGGc                                                                                                                                       >  2:162447/1‑275 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAAGGAGGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTAACGTGGTCGCCATACCGGCGACAACGTGAAc                                                                                                                                                                                                                                                                  >  8:1280772/1‑149 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                                                                                                                                                  <  6:154386/149‑1 (MQ=255)
                                                                                                           aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCAACAACTTACACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCCGATAGCCGTTGCCCGACCGACAGGATTATCTTCCGGccca                                                                                                                                    >  2:340543/1‑275 (MQ=255)
                                                                                                                gTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGa                                                                                                                                                                                                                                                             <  8:457603/149‑1 (MQ=255)
                                                                                                                 tttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGAt                                                                                                                                                                                                                                                            >  5:1068796/1‑149 (MQ=255)
                                                                                                                 tttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGAt                                                                                                                                                                                                                                                            >  5:83847/1‑149 (MQ=255)
                                                                                                                  ttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                                                                                                                                                           <  6:1187209/149‑1 (MQ=255)
                                                                                                                    cGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGc                                                                                                                                                                                                                                                         <  6:309988/149‑1 (MQ=255)
                                                                                                                         gCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACTTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAACGCCCCAGTACCg                                                                                                                      >  2:336983/1‑275 (MQ=255)
                                                                                                                          ccGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                                                                                                                                                   >  7:316801/1‑149 (MQ=255)
                                                                                                                          ccGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                                                                                                                                                   <  7:697685/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                                 <  7:608378/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                                 <  6:996673/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                                 <  7:967593/149‑1 (MQ=255)
                                                                                                                            gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                                 >  8:680308/1‑149 (MQ=255)
                                                                                                                               gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                                                                                                                                              <  8:316801/149‑1 (MQ=255)
                                                                                                                               gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                                                                                                                                              >  8:773825/1‑149 (MQ=255)
                                                                                                                                     tGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgccgcc                                                                                                                                                                                                                                        >  7:496966/1‑149 (MQ=255)
                                                                                                                                     tGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgccgcc                                                                                                                                                                                                                                        <  8:1085233/149‑1 (MQ=255)
                                                                                                                                          gTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                                                                                                                                                   >  5:57227/1‑149 (MQ=255)
                                                                                                                                            gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                                                                                                                                                 <  7:200362/149‑1 (MQ=255)
                                                                                                                                            gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                                                                                                                                                 <  6:179447/149‑1 (MQ=255)
                                                                                                                                             ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATAACGGCGACAACGTTAACGAAGATGGCTATTACTGGATGCCGCCAATGCGACTTAAGCGCAGACCCGTCGGCCATTCGCCTTCTTTCCTATCGAACCACATCCCGTTAGCCGTTCCCAGCCCACCCCGCTCCCCTTCCCGCACAACCCCCCCGCCACTGCTACCCGCGCttgttccc                                                                                                   >  4:8597/1‑267 (MQ=255)
                                                                                                                                             ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                                                                                                                                                           >  2:82442/1‑122 (MQ=255)
                                                                                                                                             ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGAc                                                                                                                                                                                                                                <  6:356525/149‑1 (MQ=255)
                                                                                                                                                aCCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTa                                                                                                                                                                                                                             >  5:790686/1‑149 (MQ=255)
                                                                                                                                                 ccTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTa                                                                                                                                                                                                                                                       <  4:82442/122‑1 (MQ=255)
                                                                                                                                                  cTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                                                                                                                                                           >  7:545608/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGTTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                          >  8:1146367/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                          >  8:847891/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                          >  5:520742/1‑149 (MQ=255)
                                                                                                                                                   ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                                                                                                                                          >  8:769858/1‑149 (MQ=255)
                                                                                                                                                        cTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGa                                                                                                                                                                                                                     <  7:773825/149‑1 (MQ=255)
                                                                                                                                                        cTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGcc                                                                                        <  2:39818/274‑1 (MQ=255)
                                                                                                                                                         ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAc                                                                                                                                                                                                                    >  8:385040/1‑149 (MQ=255)
                                                                                                                                                           cGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACCGCTGTCCGGCCCGTTTTCCCGGTTTTCCCCcaac                                                                                    >  4:147086/1‑273 (MQ=255)
                                                                                                                                                              gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGt                                                                                                                                                                                                                                                                      <  5:193734/94‑1 (MQ=255)
                                                                                                                                                                 aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                                                                                                                                                        >  5:323338/1‑105 (MQ=255)
                                                                                                                                                                   tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCa                                                                                                                                                                                                          <  5:1231829/149‑1 (MQ=255)
                                                                                                                                                                     ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                                        >  5:1115870/1‑149 (MQ=255)
                                                                                                                                                                     ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                                        <  6:83847/149‑1 (MQ=255)
                                                                                                                                                                        tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                                                                                                                                                     >  5:780440/1‑149 (MQ=255)
                                                                                                                                                                               aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                                                                                                                                              >  7:560280/1‑149 (MQ=255)
                                                                                                                                                                                acgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                                                                                                                                                             <  8:499893/149‑1 (MQ=255)
                                                                                                                                                                                    cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCca                                                                                      >  4:53864/1‑248 (MQ=255)
                                                                                                                                                                                       gggATGTCTTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCCCATCCAGATAGCCGTTGCCCGACCGACCAGATCCGCTTCCGGCACACAGCCCCAGTAACGGTTGTCCGACCTGTTGTCGCGGTTGTCGCCCATCACGAAGATTTTTCCCGGGGAAACaacac                                                        >  4:34191/1‑272 (MQ=255)
                                                                                                                                                                                        ggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                     <  6:1115870/149‑1 (MQ=255)
                                                                                                                                                                                            gTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgac                                                                                                                                                           >  2:120365/1‑171 (MQ=255)
                                                                                                                                                                                              cGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCAt                                                                                                                                                                               <  8:805754/149‑1 (MQ=255)
                                                                                                                                                                                               gTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGCTGGCTATTAATGGATGCCTCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATc                                                                                                                                                                              >  7:893942/1‑149 (MQ=255)
                                                                                                                                                                                                ttAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                                       <  4:120365/171‑1 (MQ=255)
                                                                                                                                                                                                  aGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGACGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTCTTGTCCCGGAGGAAAAATCCCGTTTGCCAg                                              >  2:184524/1‑274 (MQ=255)
                                                                                                                                                                                                   gCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                                                                                                                                                          <  7:769858/149‑1 (MQ=255)
                                                                                                                                                                                                          gagaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                                                                                                                                                   >  7:380180/1‑149 (MQ=255)
                                                                                                                                                                                                           agaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGtt                                                                                                                                                                  <  6:780440/149‑1 (MQ=255)
                                                                                                                                                                                                           agaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGtt                                                                                                                                                                  <  8:545608/149‑1 (MQ=255)
                                                                                                                                                                                                             aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGc                                                                                                                                                                <  6:520742/149‑1 (MQ=255)
                                                                                                                                                                                                             aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGc                                                                                                                                                                <  7:680308/149‑1 (MQ=255)
                                                                                                                                                                                                                tttctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGATCATCCAGATAGCCGTTGCccg                                                                                                                                                             >  5:1357683/3‑149 (MQ=255)
                                                                                                                                                                                                                  tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgac                                                                                                                                                           >  8:605892/1‑149 (MQ=255)
                                                                                                                                                                                                                    tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                                                                                                                                                         >  5:35590/1‑149 (MQ=255)
                                                                                                                                                                                                                    tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                                                                                                                                                         >  5:22481/1‑149 (MQ=255)
                                                                                                                                                                                                                     cATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAg                                                                                                                                                                       <  6:1339269/134‑1 (MQ=255)
                                                                                                                                                                                                                     cATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAg                                                                                                                                                                       >  5:1339269/1‑134 (MQ=255)
                                                                                                                                                                                                                      ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                                       >  8:1278164/1‑149 (MQ=255)
                                                                                                                                                                                                                        aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                                                                                                                                                     <  6:365215/149‑1 (MQ=255)
                                                                                                                                                                                                                        aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                                                                                                                                                     >  6:746497/1‑149 (MQ=255)
                                                                                                                                                                                                                         aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTc                                                                                                                                                                                                       >  7:552692/1‑98 (MQ=255)
                                                                                                                                                                                                                         aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTc                                                                                                                                                                                                       <  8:552692/98‑1 (MQ=255)
                                                                                                                                                                                                                         aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAg                                                                                                                                                    >  7:1329147/1‑149 (MQ=255)
                                                                                                                                                                                                                             gttCACGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                                >  6:303405/2‑149 (MQ=255)
                                                                                                                                                                                                                             atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCCACTTAAGCCCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAACCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCCTCCGGCACAAAGCCCCAGTCACCGCTGTCCGCGCTGTCGTCCCGGTTGTCGCCCATCATGAAGTATAGTCCCGGTCGAATAATCCAGGTTGCCAGGTGTTGCCCTGGCTGCTGGAAATACAt                   >  4:39251/1‑274 (MQ=255)
                                                                                                                                                                                                                              ttCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCg                                                                                                                                               >  7:15593/1‑149 (MQ=255)
                                                                                                                                                                                                                               tCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATTGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCTACCAGATTCGc                                                                                                                                              <  7:1278164/149‑1 (MQ=255)
                                                                                                                                                                                                                                cACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAGGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCACATCATGAAg                                                                              >  2:96023/1‑212 (MQ=255)
                                                                                                                                                                                                                                 aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCtt                                                                                                                                            >  7:440414/1‑149 (MQ=255)
                                                                                                                                                                                                                                    ttGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg                                                                                                                                         >  5:422053/1‑149 (MQ=255)
                                                                                                                                                                                                                                      gTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCcatcatta                                                                                >  2:254943/1‑202 (MQ=255)
                                                                                                                                                                                                                                       tCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATACCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                                >  8:1342771/1‑139 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTc                                                                                                                >  2:345206/1‑169 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCAGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                     >  8:488927/1‑100 (MQ=255)
                                                                                                                                                                                                                                         gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCAGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                                     <  7:488927/100‑1 (MQ=255)
                                                                                                                                                                                                                                          ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTa                                                                            <  4:254943/204‑1 (MQ=255)
                                                                                                                                                                                                                                           cATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa                                                                                                                                  >  6:804033/1‑149 (MQ=255)
                                                                                                                                                                                                                                             tAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATcc                 <  2:45520/260‑1 (MQ=255)
                                                                                                                                                                                                                                             tAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAAACATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCCACTGATCCTgag  >  2:149822/1‑273 (MQ=255)
                                                                                                                                                                                                                                               aCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGccc                                                                                                                              <  5:205358/149‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
TACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCG  >  NC_000913/2704068‑2704579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: