Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,707,321 A→T 6.0% intergenic (‑197/+1) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,707,3210AT 6.0% 708.8 / 25.7 203intergenic (‑197/+1)lepA/rseCback‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base A (108/80);  new base T (3/9);  total (112/91)
Fisher's exact test for biased strand distribution p-value = 3.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 209 total aligned reads displayed.

CGGTCAGACAGCGTCGATTTACCGTGGTCAATGTGAGCTATGATCGAAAAGTTACGTATATTCTTCATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAGCAGGC  >  NC_000913/2707057‑2707584
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                        
             tccaTTTGCCGTGGGCAAGTTATGCTATTATTGAAAAGTTACGTTAATTTTTCATAAAGTATGATTATTTTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAg                                                                                                                                                                                                                                                   <  4:322464/272‑1 (MQ=255)
                                                                                                                        cTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCAc                                                                                                                                                                                                                                                                      >  5:853875/1‑149 (MQ=255)
                                                                                                                              tGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtt                                                                                                                                                                                                                                                                <  6:862916/149‑2 (MQ=255)
                                                                                                                              tGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTc                                                                                                                                                                                                                                                                >  6:1095370/1‑149 (MQ=255)
                                                                                                                               gAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                                                                                                                                                                                               <  5:1314819/149‑3 (MQ=255)
                                                                                                                               gAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                                                                                                                                                                                               <  8:469061/149‑3 (MQ=255)
                                                                                                                                  cTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgtt                                                                                                                                                                                                                                                            <  7:273066/149‑6 (MQ=255)
                                                                                                                                      aCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTc                                                                                                                                                                                                                                                        <  5:443192/149‑1 (MQ=255)
                                                                                                                                        gTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCg                                                                                                                                                                                                                                                      >  7:577246/1‑149 (MQ=255)
                                                                                                                                           tGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgt                                                                                                                                                                                                                                                             <  8:511380/139‑5 (MQ=255)
                                                                                                                                           tGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgt                                                                                                                                                                                                                                                             >  7:511380/1‑135 (MQ=255)
                                                                                                                                             tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGAt                                                                                                                                                                                                                                                 >  7:93411/1‑149 (MQ=255)
                                                                                                                                              aTTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATg                                                                                                                                                                                                                                                >  5:728375/1‑149 (MQ=255)
                                                                                                                                               ttAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGt                                                                                                                                                                                                                                               >  5:781050/1‑149 (MQ=255)
                                                                                                                                                tAATAGAAACGCCCATTTAAAAACTACAACGTTTGGCCGAGGAAATATTCATAACTTAGGGTTTGGGGTATCTGGAAACTTCCTCTCATTTGTTATGCAAAATGCAACAAAGCCAGTGAACTCCCGGGCTCCCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGca                                                                                                                <  4:238906/275‑1 (MQ=255)
                                                                                                                                                  aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTc                                                                                                                                                                                                                                            >  8:699019/1‑149 (MQ=255)
                                                                                                                                                  aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTc                                                                                                                                                                                                                                            >  5:614160/1‑149 (MQ=255)
                                                                                                                                                   tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCa                                                                                                                                                                                                                                           >  6:1196370/1‑149 (MQ=255)
                                                                                                                                                   tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCa                                                                                                                                                                                                                                           >  8:1347546/1‑149 (MQ=255)
                                                                                                                                                    aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaa                                                                                                                                                                                                                                          >  7:1078968/1‑149 (MQ=255)
                                                                                                                                                    aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaa                                                                                                                                                                                                                                          >  6:122457/1‑149 (MQ=255)
                                                                                                                                                    aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaa                                                                                                                                                                                                                                          >  5:195390/1‑149 (MQ=255)
                                                                                                                                                    aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaa                                                                                                                                                                                                                                          >  5:1255339/1‑149 (MQ=255)
                                                                                                                                                     gAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaaa                                                                                                                                                                                                                                         <  8:1224912/149‑1 (MQ=255)
                                                                                                                                                      aaaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAAt                                                                                                                                                                                                                                        <  6:1458290/149‑1 (MQ=255)
                                                                                                                                                          gccgcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                                                                                                                                                                                                    <  8:93411/149‑1 (MQ=255)
                                                                                                                                                            cgcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCAAAAGGCCAGGCGGCAAGGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGAAGGAAACCTACGCAAGTAGCCGGCAGACATCAGGAACCCGCCAATACCACCGAGGATAGcacccaaatatgct                                                                                                    >  4:53234/1‑265 (MQ=255)
                                                                                                                                                             gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                                                                                                                                                                                                  <  7:475331/132‑1 (MQ=255)
                                                                                                                                                             gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACaa                                                                                                                                                                                                                                 >  7:358269/1‑149 (MQ=255)
                                                                                                                                                              cATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAg                                                                                                                                                                                                                                <  7:1436186/149‑1 (MQ=255)
                                                                                                                                                                   tacactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAg                                                                                                                                                                                                                           >  5:915294/1‑149 (MQ=255)
                                                                                                                                                                       ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                                                                                                                                                                                       >  7:704594/1‑149 (MQ=255)
                                                                                                                                                                              aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGcc                                                                                                                                                                                                                >  6:127471/1‑149 (MQ=255)
                                                                                                                                                                              aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGcc                                                                                                                                                                                                                >  7:156830/1‑149 (MQ=255)
                                                                                                                                                                               ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGc                                                                                                                                                                                                                                                                  <  7:286319/98‑1 (MQ=255)
                                                                                                                                                                               ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCa                                                                                                                                                                                                               >  8:590652/1‑149 (MQ=255)
                                                                                                                                                                                tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                                                                                                                                                                                              <  6:915294/149‑1 (MQ=255)
                                                                                                                                                                                tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                                                                                                                                                                                              <  6:614160/149‑1 (MQ=255)
                                                                                                                                                                                       aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGACCGCAGCACATAATGCTGCGACGTCAGAAGAAAAGAGTAACTGa                                                                         >  4:35092/1‑275 (MQ=255)
                                                                                                                                                                                       aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAAGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATCACACCGCGGATCGCACCACATAAGGCTGCGACGTCAGAAGCAGAGAGAAGCGGa                                                                         >  1:8165/1‑275 (MQ=255)
                                                                                                                                                                                        ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAga                                                                                                                                                                                                      >  7:763061/1‑149 (MQ=255)
                                                                                                                                                                                              gTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg                                                                                                                                                                                                >  6:1179062/1‑149 (MQ=255)
                                                                                                                                                                                                tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt                                                                                                                                                                                              >  8:1080022/1‑149 (MQ=255)
                                                                                                                                                                                                 cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGtt                                                                                                                                                                                             <  5:122457/149‑1 (MQ=255)
                                                                                                                                                                                                      cGTATGGATTGTGGTATCTGGAAACTTCCTCCCATTTGTTAGGCAAAATGCAACCAAGCCATTGAAATCACTGGCTGGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGAt                                                           <  2:53234/274‑1 (MQ=255)
                                                                                                                                                                                                       gTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGt                                                                                                                                                                                                                                               <  6:444028/93‑1 (MQ=255)
                                                                                                                                                                                                        tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAg                                                        >  2:174407/1‑275 (MQ=255)
                                                                                                                                                                                                        tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGACCGCACCACCTAATGCGTCGACGGCAGAAACAAAGAGTCGCTAAAATagccaacggaaaa                                                         >  4:278963/1‑267 (MQ=255)
                                                                                                                                                                                                         aTGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGCTGATCGGTTGCCATTCTGCCCGCGCAGCAAACTTACGCGAGTAGCCGCGCTCAATCAGGAACCCGCCAATACCACCGACGAACGCACCACATAATGCTGCGACGTCCGAAGCAAAGAGTAGCTGCAATAGCGAAGCGGTAAg                                                        >  1:64898/1‑274 (MQ=255)
                                                                                                                                                                                                         aTGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACg                                                                                              >  4:88982/1‑236 (MQ=255)
                                                                                                                                                                                                           ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc                                                                                                                                                                                   <  8:531733/149‑1 (MQ=255)
                                                                                                                                                                                                            gagtgtgGTAGCTGGAAACTTCCTCGCATTTGTTATGCAAAATGCAACAAGGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGAt                                                           <  2:278963/265‑1 (MQ=255)
                                                                                                                                                                                                             aTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcg                                                                                                                                                       >  2:39957/1‑175 (MQ=255)
                                                                                                                                                                                                             aTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAg                                                                                          <  2:88982/236‑1 (MQ=255)
                                                                                                                                                                                                              ttGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg                                                                                                                                                                                <  5:1191815/149‑1 (MQ=255)
                                                                                                                                                                                                                 tgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAGCGCACCACATAATGCTGCGACGTCAGAAGCAAAGCGTAGCTGAAATagcgaagc                                                              >  1:44087/1‑260 (MQ=255)
                                                                                                                                                                                                                       cgggAAACGTCCTGCAATTTGTTATGAAAAATGAAAAAAAGCCAGTGAAATCCCTGGCTAGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGAGGCAATGCCACGCTTAAGGTGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTCCGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGAACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAg                                         <  4:174407/273‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCaaa                                                                                                                                                                       <  8:128805/149‑1 (MQ=255)
                                                                                                                                                                                                                            aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAAGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAc                                                                                                                                                                  <  7:1208979/149‑1 (MQ=255)
                                                                                                                                                                                                                            aaCGCCCGCCCATTTTTTATGCAAAATGCAACAAAGCGAGTGAAACCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTGGCCATTCTGCCCGGGCAGCAAACTTACGCAGGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAgcggcg                                   <  2:35092/275‑1 (MQ=255)
                                                                                                                                                                                                                               gTCCTCGCATTTTTTATGCAAAATGCAACAAAGCGAGTGAAATCACTGGCACGCGTCTTCGGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGGTGATCGGTGGCAATTCTGCCCGGGCAGCAAACTTACGCGAGTAGAcgcg                                                                                                                                                     <  2:133537/159‑1 (MQ=255)
                                                                                                                                                                                                                               gTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgcg                                                                                                                                                               >  6:28300/1‑149 (MQ=255)
                                                                                                                                                                                                                                tCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCCCAAGGCCAGGCGGCAATGCCACGCTGAAGATGATCGGTGGCCATTCTGCCCGGGCAGCAAACTTACGCGa                                                                                                                                                              <  8:358269/149‑1 (MQ=255)
                                                                                                                                                                                                                                tCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGa                                                                                                                                                              <  5:1415209/149‑1 (MQ=255)
                                                                                                                                                                                                                                tCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGa                                                                                                                                                              <  7:1083322/149‑1 (MQ=255)
                                                                                                                                                                                                                                tCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGACAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgcaa                                                                                                                                                              <  8:760034/149‑3 (MQ=255)
                                                                                                                                                                                                                                 ccTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAg                                                                                                                                                             >  8:494439/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGt                                                                                                                                                            >  7:1001168/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTTTGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCGGGGCAGCAAACTTACGCGAGTa                                                                                                                                                           >  7:825082/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGTGGCAATGCCACGCTTAAga                                                                                                                                                                                                      <  8:1007277/106‑1 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAga                                                                                                                                                                                                      >  7:1007277/1‑106 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTa                                                                                                                                                           >  5:1444646/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTa                                                                                                                                                           >  7:1306346/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTa                                                                                                                                                           >  6:251098/1‑149 (MQ=255)
                                                                                                                                                                                                                                    cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAg                                                                                                                                                          <  8:778233/149‑1 (MQ=255)
                                                                                                                                                                                                                                        tttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatga                                                                                                                                                                                                   >  8:36831/1‑104 (MQ=255)
                                                                                                                                                                                                                                        tttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcgc                                                                                                                                                      >  5:1167215/1‑149 (MQ=255)
                                                                                                                                                                                                                                          tGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcgcgc                                                                                                                                                    <  6:781050/149‑1 (MQ=255)
                                                                                                                                                                                                                                          tGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATGGCACCACATAATGCTGCGACGTAGAAAGCAAAGAGTAGCCGAAATGAAGAAGGGAAAAGGAATAAAACCACCAGCGGCCGACataaagacagg                      >  4:72603/1‑267 (MQ=255)
                                                                                                                                                                                                                                          ggttagccAAAATCCACAAAAGACAGTGAAGCCCCTGGCCCGCGTCTTCTGAAGATTTTTCAAATCGCACAAGGCCGNGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATGCTGCCCGGGCAGCAAGCTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGt                     <  3:65476/268‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt                                                                                                                                                                                                                                              >  8:888213/1‑54 (MQ=255)
                                                                                                                                                                                                                                               tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAAt                                                                                                                                               <  5:1294217/149‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAAt                                                                                                                                               >  7:43483/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cAAAATGCAACAAAGCCAGTGAAATAACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCa                                                                                                                                             <  7:953827/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTGCAACAAATCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTACAAATCGCACAAGGCCAGGCGCCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAg                                                                                                                                            >  8:206797/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAg                                                                                                                                            >  6:632627/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAg                                                                                                                                            >  7:421461/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAg                                                                                                                                            <  8:156830/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGAGCAATCAg                                                                                                                                            <  5:103061/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGa                                                                                                                                          >  8:284017/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGaa                                                                                                                                         >  5:1333299/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGaa                                                                                                                                         >  5:32058/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       gCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggc                                                                                                                                                                                                                         <  8:666137/67‑1 (MQ=255)
                                                                                                                                                                                                                                                       gCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggc                                                                                                                                                                                                                         >  7:666137/1‑67 (MQ=255)
                                                                                                                                                                                                                                                              aGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAAt                                                                                                                                >  5:354088/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              aGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGATAAGCAGGc  >  2:302941/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                ccAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATac                                                                                                                              <  5:28300/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAACCAGGAACCCGCCAATaccac                                                                                                                           >  7:21312/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       aaTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGtt                                                                                                                                                                                             >  7:1132120/1‑79 (MQ=255)
                                                                                                                                                                                                                                                                       aaTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGtt                                                                                                                                                                                             <  8:1132120/79‑1 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                        
CGGTCAGACAGCGTCGATTTACCGTGGTCAATGTGAGCTATGATCGAAAAGTTACGTATATTCTTCATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGATCGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAGCAGGC  >  NC_000913/2707057‑2707584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: