Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,873,917 G→C 6.6% H26D (CAT→GAT) ‡ cysC ← adenosine 5'‑phosphosulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,873,9170GC 6.6% 714.4 / 32.8 206H26D (CAT→GAT) ‡cysCadenosine 5'‑phosphosulfate kinase
Reads supporting (aligned to +/- strand):  ref base G (93/97);  new base C (6/8);  total (100/106)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

Only 100 of 207 total aligned reads displayed.

GTTCGGCGCGGTGTGGCGAGATAAATGCGGTCAGCACCACCAGTCCGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGATCGATAAAAATCAGCCCACCCGTCACCGGATTTTGT  >  NC_000913/2873650‑2874190
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                 
         ggTGTGGCGAGATAAATGCGGTCAGCACCACCAGTCCGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTcg                                                                                                                                                                                                                                                                   <  2:280673/275‑1 (MQ=255)
                             gTCAGCACCACCAGTCCGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTGGCTTTACCGGACCCGGAGAGGCCGGTAAACCCAAGCACTACCCCCCGCACCACGGGGGGTAGCTCGGCTTGTTTCACAGGGACCgg                                                                                                                                                                                                                                               >  4:334332/1‑275 (MQ=255)
                                 gCACCACCAGTCCGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGca                                                                                                                                                                                                                                                         <  2:334332/261‑1 (MQ=255)
                                            ccGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAg                                                                                                                                                                                                                                                                       >  4:12266/1‑236 (MQ=255)
                                                                                                                       agaTCGCTGCAGACACCGTGGCGAACATTCACGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCGCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGTAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcgacg                                                                                                                                                      <  2:318439/274‑1 (MQ=255)
                                                                                                                           cGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                                                                                                                                                            <  4:213465/168‑1 (MQ=255)
                                                                                                                               gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                                                                                                                                                           <  8:1349904/149‑1 (MQ=255)
                                                                                                                                  tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                                                                                                                                                        <  5:611360/149‑1 (MQ=255)
                                                                                                                                  tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                                                                                                                                                        <  6:1424209/149‑1 (MQ=255)
                                                                                                                                    aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTAgt                                                                                                                                                                                                                                                                      <  7:660136/149‑2 (MQ=255)
                                                                                                                                    aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGc                                                                                                                                                                                                                                                                      <  6:63168/149‑1 (MQ=255)
                                                                                                                                    aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGc                                                                                                                                                                                                                                                                      >  5:44862/1‑149 (MQ=255)
                                                                                                                                          ggCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgt                                                                                                                                                                                                                                                                <  6:1003347/149‑1 (MQ=255)
                                                                                                                                          ggCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTCTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACCAACCAGAGCATTCAATTCCa                                                                                                                                  >  2:185880/1‑275 (MQ=255)
                                                                                                                                             gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGAcc                                                                                                                                                                                                                                                                                <  6:992398/130‑1 (MQ=255)
                                                                                                                                              aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                                                                                                                                                            <  6:670984/149‑1 (MQ=255)
                                                                                                                                              aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                                                                                                                                                            <  5:1018178/149‑1 (MQ=255)
                                                                                                                                                     tCGCCATCCAGCAGATACGTACTGACGCCCAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCa                                                                                                                       <  2:38671/275‑1 (MQ=255)
                                                                                                                                                        ccATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGAc                                                                                                                                                                                                                                                  <  7:785707/149‑1 (MQ=255)
                                                                                                                                                         cATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGAcc                                                                                                                                                                                                                                                 >  6:43545/1‑149 (MQ=255)
                                                                                                                                                         cATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGAcc                                                                                                                                                                                                                                                 <  6:525536/149‑1 (MQ=255)
                                                                                                                                                           tCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCgg                                                                                                                                                                                                                                               >  5:244010/1‑149 (MQ=255)
                                                                                                                                                             cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                                                                                                                                                             <  8:699075/149‑1 (MQ=255)
                                                                                                                                                             cagcagATACGTACTGACGCCGAATTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                                                                                                                                                           <  7:950945/119‑1 (MQ=255)
                                                                                                                                                                   aTACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCg                                                                                                                                                                                                                                                >  5:1473531/1‑140 (MQ=255)
                                                                                                                                                                   aTACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacg                                                                                                                                                                                                                               <  1:53701/157‑1 (MQ=255)
                                                                                                                                                                          cTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgac                                                                                                                                                                                                                                >  6:793128/1‑149 (MQ=255)
                                                                                                                                                                           tGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtgg                                                                                                                                                                                                                                                                            <  7:975909/104‑1 (MQ=255)
                                                                                                                                                                             aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacgac                                                                                                                                                                                                                             <  6:44862/149‑1 (MQ=255)
                                                                                                                                                                             aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTg                                                                                                                                                                     >  2:87024/1‑205 (MQ=255)
                                                                                                                                                                             aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGt                                                                                                >  2:206330/1‑274 (MQ=255)
                                                                                                                                                                               gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                                                                                                                                                           <  6:1183347/149‑1 (MQ=255)
                                                                                                                                                                               gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                                                                                                                                                           >  5:756042/1‑149 (MQ=255)
                                                                                                                                                                                ccGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGca                                                                                                                                                                                                                                                         >  5:872685/1‑118 (MQ=255)
                                                                                                                                                                                ccGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtt                                                                                                                                                                                                                          >  7:471182/1‑149 (MQ=255)
                                                                                                                                                                                 cGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGttt                                                                                                                                                                                                                         <  7:1324656/149‑1 (MQ=255)
                                                                                                                                                                                 cGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGa                                                                                                                                                                 <  4:87024/205‑1 (MQ=255)
                                                                                                                                                                                   aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                                                                                                                                                       <  6:1073524/149‑1 (MQ=255)
                                                                                                                                                                                   aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCATGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                                                                                                                                                       <  7:776493/149‑1 (MQ=255)
                                                                                                                                                                                     tttATGTAACGCCTCCTCCATCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACTGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                                                                                                                                                     >  8:797731/1‑149 (MQ=255)
                                                                                                                                                                                     tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTc                                                                                                                                                                                                                                                                    <  8:387259/102‑1 (MQ=255)
                                                                                                                                                                                     tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTc                                                                                                                                                                                                                                                                    >  7:387259/1‑102 (MQ=255)
                                                                                                                                                                                     tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                                                                                                                                                     >  7:106326/1‑149 (MQ=255)
                                                                                                                                                                                        aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                                                                                                                                                        <  6:173929/95‑1 (MQ=255)
                                                                                                                                                                                         tGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACt                                                                                   <  2:170478/275‑1 (MQ=255)
                                                                                                                                                                                         actctccagtactccAGCTCCCCGGCCAACATTGATTTACCGGACCCGGAGGGGTCGGTAAACCACAGCACTACACCCCTATGACCGTGGTGTAGCTCGCGTTGCTGCACAGTGACCGGATTGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCGAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACt                                                                                   <  1:31813/264‑1 (MQ=255)
                                                                                                                                                                                          gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                                                                                                                                                    >  6:860621/1‑145 (MQ=255)
                                                                                                                                                                                          gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                                                                                                                                                    <  5:860621/145‑1 (MQ=255)
                                                                                                                                                                                            aaCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTAgt                                                                                                                                                                                                                                                                      >  7:13053/1‑92 (MQ=39)
                                                                                                                                                                                            aaCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTAgt                                                                                                                                                                                                                                                                      <  8:13053/93‑2 (MQ=39)
                                                                                                                                                                                            aaCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTAgt                                                                                                                                                                                                                                                                      <  8:1160992/93‑2 (MQ=38)
                                                                                                                                                                                             aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCa                                                                                                                                                                                                                                   >  6:255353/1‑127 (MQ=255)
                                                                                                                                                                                             aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                                                                                                                                                             >  7:1267838/1‑149 (MQ=255)
                                                                                                                                                                                             aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTCGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                                                                                                                                                             >  8:1101686/1‑149 (MQ=255)
                                                                                                                                                                                               gCCTCTCCCAGCGCCCCGGGGACCGGTGATTGACCGGACCCGGAGAGGCCGGTAAACCAAAGAACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCg                                                                             <  4:302378/275‑1 (MQ=255)
                                                                                                                                                                                               gCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgcgc                                                                                                                                                                                                           <  6:747981/149‑1 (MQ=255)
                                                                                                                                                                                               gCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgcgc                                                                                                                                                                                                           >  8:394751/1‑149 (MQ=255)
                                                                                                                                                                                                 ctcctcCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCa                                                                                                                                                                                                         >  6:1482476/1‑149 (MQ=255)
                                                                                                                                                                                                  tcctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCat                                                                                                                                                                                                        >  8:652476/1‑149 (MQ=255)
                                                                                                                                                                                                     tccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                                                                                                                                                     <  5:185789/149‑1 (MQ=255)
                                                                                                                                                                                                     tccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                                                                                                                                                     <  7:170261/149‑1 (MQ=255)
                                                                                                                                                                                                     tccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                                                                                                                                                     <  5:1132289/149‑1 (MQ=255)
                                                                                                                                                                                                     tccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                                                                                                                                                     <  8:199689/149‑1 (MQ=255)
                                                                                                                                                                                                         gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttatttat                                                                                                                                                                                                 >  7:1184702/1‑149 (MQ=255)
                                                                                                                                                                                                         gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttatttat                                                                                                                                                                                                 <  5:793128/149‑1 (MQ=255)
                                                                                                                                                                                                           gcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                                                                                                                                                               <  6:1296970/149‑1 (MQ=255)
                                                                                                                                                                                                              ccGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATccccc                                                                                                                                                                                            <  5:159539/149‑1 (MQ=255)
                                                                                                                                                                                                                ggCGACCGTTGATTTACCGGACCCGGGGAGGCCGGGAAACAACAGGACAAAACCACGTTGACCGTGGGGTAGCTGGCGTTGTGGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGGGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCAcc                                                            <  4:188293/275‑1 (MQ=255)
                                                                                                                                                                                                                      cGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                                                                                                                                                    >  5:59994/1‑149 (MQ=255)
                                                                                                                                                                                                                       gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                                                                                                                                                                   >  7:879309/1‑149 (MQ=255)
                                                                                                                                                                                                                          gATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgc                                                                                                                                                                                >  5:807352/1‑149 (MQ=255)
                                                                                                                                                                                                                                 cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcccc                                                                                                                                                                                             <  8:764361/129‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGc                                                                                                                                                                    <  7:1350002/149‑1 (MQ=255)
                                                                                                                                                                                                                                       cGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCGCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGcgat                                     <  2:277155/275‑1 (MQ=255)
                                                                                                                                                                                                                                              gCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTg                                                                                                                                                            <  5:687794/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 ggTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcg                                                                                                                                                         >  8:939889/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   tAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcgac                                                                                                                                                       >  8:287226/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   tAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcgac                                                                                                                                                       <  5:1283725/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTCGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcgacga                                                                                                                                                     >  7:39728/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       ccACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAc                                                                                                                                                   <  8:1115231/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                                                                                                                                                      >  8:156138/1‑97 (MQ=255)
                                                                                                                                                                                                                                                        cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                                                                                                                                                      <  7:156138/97‑1 (MQ=255)
                                                                                                                                                                                                                                                           aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga                                                                                                                                               >  5:1438770/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            gCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCagag                                                                                                                                              <  7:1108779/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCa                                                                                                                                            >  5:130965/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCa                                                                                                                                            <  8:1184702/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCa                                                                                                                                            >  8:716254/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATc                                                                                                                                                                                                >  6:321259/1‑95 (MQ=255)
                                                                                                                                                                                                                                                                tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATc                                                                                                                                                                                                <  5:321259/95‑1 (MQ=255)
                                                                                                                                                                                                                                                                  caccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCCCACGCGAACGATAAAAATCAGCCCCCCCGCCACCg          >  4:203551/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    ccacGATGACCGTGGTGTAGCTCTCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAt                                                                                                                                      >  5:390543/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAt                                                                                                                                      >  8:465047/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAtt                                                                                                                                     >  7:394350/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAtt                                                                                                                                     >  6:883644/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAtt                                                                                                                                     >  5:912638/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAtt                                                                                                                                     >  5:1355897/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      acGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTc                                                                                                                                    <  5:583250/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCa                                                                                                                                  <  5:43545/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                          tGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGt                                                                                                                                <  5:138983/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gACCCTGGTGTAGCTCGCGCTTTTGCACAGTGTCCGGATGGCTATCCCGGACGACGTTTTCGTCATGCAGCGCCATTCTTTATCCCCCAGCAAATCCCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGtt                                                                                                                               >  6:388669/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                 
GTTCGGCGCGGTGTGGCGAGATAAATGCGGTCAGCACCACCAGTCCGGCTTCAACCATCAAATTCGCCACTTCACCGACGCGACGGATATTCTCTTTACGATCGGCATCGCTAAAACCGAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGATCGATAAAAATCAGCCCACCCGTCACCGGATTTTGT  >  NC_000913/2873650‑2874190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: