Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,020,512 A→C 5.9% intergenic (+23/‑28) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,020,5120AC 5.9% 619.8 / 24.4 192intergenic (+23/‑28)ssnA/ygfMputative chlorohydrolase/aminohydrolase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (79/93);  new base C (7/4);  total (89/103)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

Only 100 of 195 total aligned reads displayed.

CTTCTGGAAGCCGGTTACAAAGCTGATTTAACCATTTGCGATTACAACTCGCCGACGCCGCTGCTGGCAGACAATATCGCCGGGCATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCG  >  NC_000913/3020244‑3020773
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                     
cTTTTGGGAGCAGGTTACAAAGCGGATGTAACCATGGGCGTGTACAGCTAGCCGGGGCCGATGCTGGCAGACAATATCGCCGGGCATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGGTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGaa                                                                                                                                                                                                                                                                  <  4:288903/274‑1 (MQ=255)
           cGGTTACAAAGTTGATTTAACCATTGGGGATTACAACTCGCCGAGGCCGGTGCGGGCAGACAAGATCGCCGGGCATATCGCGTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATa                                                                                                                                                                                                                                                      <  4:296814/275‑1 (MQ=255)
               ttaaaagcgcgtttACCCATTTGGGATTACAACTGCCGGACCCGCCTGCTGGCAGACAATATCGCCGGGCATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGGGAGGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                                                                                                                                                                                                                                                  <  4:148795/264‑1 (MQ=255)
                                   ttGCGATTACAACTCGCCGACGCCGCTGCTGGCAGACAATATCGCCGGGCATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTGGATTGCGATTACATTTATGCACAAGCCAGAAAAGCAGCTGCGAGAATGTGGCGTCGGATGTAGGCGCTGGCATAAAGGACAGATGCCATCTTCCCGCAGGGAAGAGGGTTAAATACGGCAAGAAGATGATTGAACAAtt                                                                                                                                                                                                                              >  1:14848/1‑275 (MQ=255)
                                                                                    cATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGCTGCCCTCTTCCCGCAGGGAAGAGGGTTAAATACGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGCCGCGGAAAcgtaaactccccca                                                                                                                                                                              >  4:244922/1‑261 (MQ=255)
                                                                                    cATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAg                                                                                                                                                                                      >  4:282290/1‑266 (MQ=255)
                                                                                                                 cGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGAAGCTTGAACAATTTTTCAGGCCAGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGACACCGTCTGGTTCGCCtggg                                                                                                                                                 >  2:275563/1‑274 (MQ=255)
                                                                                                                         gCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCCCTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                                                                                                                                      <  7:1240407/149‑1 (MQ=255)
                                                                                                                               tGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                                                                                                                                                                                                                                                <  8:655655/149‑1 (MQ=255)
                                                                                                                                ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                                                                                                                                               <  5:733778/149‑1 (MQ=255)
                                                                                                                                ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                                                                                                                                                                                                                                               <  7:1345788/149‑1 (MQ=255)
                                                                                                                                ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATGCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                                                                                                                                               <  5:355645/149‑1 (MQ=255)
                                                                                                                                ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGCTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCGGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa                                                                                                                                    <  2:30937/272‑1 (MQ=255)
                                                                                                                                 gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                                                                                                                                              <  8:706799/149‑1 (MQ=255)
                                                                                                                                 gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                                                                                                                                              <  7:557343/149‑1 (MQ=255)
                                                                                                                                 gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAgg                                                                                                                                                                                                                                                              <  5:1434866/149‑1 (MQ=255)
                                                                                                                                 gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAgg                                                                                                                                                                                                                                                              <  5:923557/149‑1 (MQ=255)
                                                                                                                                    aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                                                                                                                                                                                                                                               <  6:928678/145‑1 (MQ=255)
                                                                                                                                    aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                                                                                                                                                                                                                                               >  5:928678/1‑145 (MQ=255)
                                                                                                                                    aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                                                                                                                                           >  5:1014851/1‑149 (MQ=255)
                                                                                                                                    aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                                                                                                                                           >  7:412952/1‑149 (MQ=255)
                                                                                                                                        gtgtGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                                                                                                       <  2:286951/261‑1 (MQ=255)
                                                                                                                                          gtgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATaa                                                                                                                                                                                                                                                     <  5:1068096/149‑1 (MQ=255)
                                                                                                                                           tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                                                                                                                                                    <  8:1430183/149‑1 (MQ=255)
                                                                                                                                              tgtgtgaTGAAGACCGGCAGTGTAACTTCGATTGCGCTTCCATTTATCCACAAGCCCGAAAAGGCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGCTGCCTTCTTCCCGCAGGGAAGATGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTa                                                                                                                   <  4:167351/269‑1 (MQ=255)
                                                                                                                                                  cTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaa                                                                                                                                                                                                                                             <  7:1080579/149‑1 (MQ=255)
                                                                                                                                                  cTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaa                                                                                                                                                                                                                                             <  5:281626/149‑1 (MQ=255)
                                                                                                                                                   tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                                                                                                                                                                                                                                            >  6:1412073/1‑149 (MQ=255)
                                                                                                                                                   tATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAt                                                                                                                                                                                                                               >  2:226814/1‑162 (MQ=255)
                                                                                                                                                    aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                                                                                                                                                                                                                                           >  6:913257/1‑149 (MQ=255)
                                                                                                                                                     tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                                                                                                                                          >  7:912878/1‑149 (MQ=255)
                                                                                                                                                     tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                                                                                                                                          >  6:1347344/1‑149 (MQ=255)
                                                                                                                                                     tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                                                                                                                                          <  7:884521/149‑1 (MQ=255)
                                                                                                                                                      gAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatg                                                                                                                                                                                                                                         >  8:209444/1‑149 (MQ=255)
                                                                                                                                                         gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                                                                                                                                      <  7:727406/149‑1 (MQ=255)
                                                                                                                                                         gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                                                                                                                                      <  5:311383/149‑1 (MQ=255)
                                                                                                                                                         gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                                                                                                                                      <  7:122197/149‑1 (MQ=255)
                                                                                                                                                         gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                                                                                                                                      <  6:1111228/149‑1 (MQ=255)
                                                                                                                                                         gACCGACAGTTTAAATTCGATTGCGAATCCATATATGCAGAAGCCAGAAAAGACGCTGCCAGTATGTGGAGGCGAATGGATGCGCTAGAATAAATTACAGAAGCGCTCTTCCCGCAGGGAAGAGGTTTAAATAAAGAAAGAAGATGAtt                                                                                                                                                                                                                                      <  6:865838/149‑1 (MQ=255)
                                                                                                                                                           ccGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                                                                                                                                     >  2:47606/1‑116 (MQ=255)
                                                                                                                                                             gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                                                                                                                                              <  8:792094/121‑1 (MQ=255)
                                                                                                                                                               cAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAag                                                                                                                                                                                                                                                                 <  4:47606/116‑1 (MQ=255)
                                                                                                                                                               cAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACaa                                                                                                                                                                                                                                <  8:912878/149‑1 (MQ=255)
                                                                                                                                                                  tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGTATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGCAGAGGGTTAAATAAGGAAAGAAGATGACTGAACAAttt                                                                                                                                                                                                                             >  8:1271064/1‑149 (MQ=255)
                                                                                                                                                                  tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                                                                                                                                              >  6:231771/1‑116 (MQ=255)
                                                                                                                                                                  tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCa                                                                                                                                                                                                                                                                       >  6:713151/1‑107 (MQ=255)
                                                                                                                                                                      aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                                                                                                                                         <  8:1381861/149‑1 (MQ=255)
                                                                                                                                                                      aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                                                                                                                                         <  7:784292/149‑1 (MQ=255)
                                                                                                                                                                            cttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTCCATTGCGTCGGATGGATGCCCTGGCATAAATGACAGGTGCCCCCTTCCCGCCGGGTAGAGGACTAAATCAGGAAAGTAGATGATTGAACAATTTTTCATGACATACGCTGTCTAACAGGCGCAGGAACTGAAACGCCGCTCCCCAGATGCAGCCGACTGCTGCGCCCGGGGAGGCAGACTCAACGCTACCCCAACCAGTACGGCTAACAAGCTTGACAttcagtcaaac                                                                                      >  4:80065/2‑265 (MQ=255)
                                                                                                                                                                                 gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGActct                                                                                                                                                                                                              >  8:1231673/1‑149 (MQ=255)
                                                                                                                                                                                       aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                                                                                                                                        <  6:431610/149‑1 (MQ=255)
                                                                                                                                                                                       aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACCCTCTTCCCTGCGGGAAGAggg                                                                                                                                                                                                                                                             <  5:1240612/96‑1 (MQ=255)
                                                                                                                                                                                        tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                                                                                                                                         >  6:651145/1‑131 (MQ=255)
                                                                                                                                                                                        tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                                                                                                                                         <  5:651145/131‑1 (MQ=255)
                                                                                                                                                                                         ttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACa                                                                                                                                                                                                      <  7:209444/149‑1 (MQ=255)
                                                                                                                                                                                              cacaAGTCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCGATCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGgcgc                                                                                                                                                                                                 <  8:1311063/149‑1 (MQ=255)
                                                                                                                                                                                              cacaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGgcgc                                                                                                                                                                                                 <  5:1021278/149‑1 (MQ=255)
                                                                                                                                                                                               acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                                                                                                                                                                                                                                                <  6:685189/85‑1 (MQ=255)
                                                                                                                                                                                               acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCt                                                                                                                                                                                                >  7:645973/1‑149 (MQ=255)
                                                                                                                                                                                                 aaGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTgg                                                                                                                                                                                              >  6:520055/1‑149 (MQ=255)
                                                                                                                                                                                                   gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGATGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                                                                                                                                            >  8:1050279/1‑149 (MQ=255)
                                                                                                                                                                                                   gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGAc                                                              <  2:113112/275‑1 (MQ=255)
                                                                                                                                                                                                     cAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACt                                                                                                                                                                                          >  5:1326741/1‑149 (MQ=255)
                                                                                                                                                                                                      aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGACGCCGTCTGGTTCGCCGGGCGCAGCAAACTCAATGCTACACCAACCCGTACCGATACAAAGATTGCCATTTCCTTACAGGATCTGGACCTGGACTGGGTTgcccg                                                            >  4:254082/1‑270 (MQ=255)
                                                                                                                                                                                                        aaaaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGaa                                                                                                                                                                                       <  7:1440293/149‑1 (MQ=255)
                                                                                                                                                                                                                   ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTAcc                                                                                                                                                                            >  7:1027108/1‑149 (MQ=255)
                                                                                                                                                                                                                    cAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCa                                                                                                                                                                           <  7:682505/149‑1 (MQ=255)
                                                                                                                                                                                                                     aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                                                                                                                                          >  7:527002/1‑101 (MQ=255)
                                                                                                                                                                                                                     aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                                                                                                                                          <  8:527002/101‑1 (MQ=255)
                                                                                                                                                                                                                      gTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAgg                                                                                                                                                                         >  8:1102885/1‑149 (MQ=255)
                                                                                                                                                                                                                       tATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGa                                                                                                                                                                        <  7:391406/149‑1 (MQ=255)
                                                                                                                                                                                                                       tATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGa                                                                                                                                                                        <  5:1231718/149‑1 (MQ=255)
                                                                                                                                                                                                                        aTGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                                                                                                       <  5:565979/149‑1 (MQ=255)
                                                                                                                                                                                                                           tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGaa                                                                                                                                                                    <  5:1116216/149‑1 (MQ=255)
                                                                                                                                                                                                                              cGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGcc                                                                                                                                                                 <  6:964636/149‑1 (MQ=255)
                                                                                                                                                                                                                               gTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                                                                                                                                                >  5:1037544/1‑149 (MQ=255)
                                                                                                                                                                                                                               gTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAAAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAAGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTgca                                   >  2:91894/1‑272 (MQ=255)
                                                                                                                                                                                                                                  ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAGCTGAAGCGCCGCTACCAGGATGAAGCCGTCt                                                                                                                                                             >  6:1071520/1‑149 (MQ=255)
                                                                                                                                                                                                                                  ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt                                                                                                                                                             <  5:994756/149‑1 (MQ=255)
                                                                                                                                                                                                                                           cgcTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCg                                                                                                                                                    <  5:1357573/149‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgggg                                                                                                                                                 >  6:1456570/1‑149 (MQ=255)
                                                                                                                                                                                                                                                gCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgcc                                                                                                                                                                                  <  5:801502/114‑1 (MQ=255)
                                                                                                                                                                                                                                                   tAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                                                                                                                                                      >  5:429608/1‑59 (MQ=255)
                                                                                                                                                                                                                                                    aaaTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACa                                                                                                                                                                                                      >  8:1416152/1‑90 (MQ=255)
                                                                                                                                                                                                                                                    aaaTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACa                                                                                                                                                                                                      <  7:1416152/90‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaaTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcagca                                                                                                                                           >  8:1163759/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      aTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaaa                                                                                                                                         >  5:464731/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      aTGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCt                                                                                           >  1:35135/1‑195 (MQ=255)
                                                                                                                                                                                                                                                          cAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGCTTGGCGCAATGTCTCGCTTTCAGCCattcggt        >  4:184789/1‑268 (MQ=255)
                                                                                                                                                                                                                                                           aGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTGTGGTTCGCCGGGGGCAGCAAACTCaa                                                                                                                                    <  8:74746/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              tGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGc                                                                                                                                 <  4:107664/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac                                                                                                                              >  8:583076/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ctctTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca                                                                                                                             >  6:785469/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ctctTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca                                                                                                                             >  7:295597/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca                                                                                                                             >  7:1296616/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   tctTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTacac                                                                                                                            >  6:366418/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   tctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTacac                                                                                                                            <  8:412952/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAc                                                                                                                        <  7:1028373/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAcc                                                                                                                       >  5:689143/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc                                                                                                                      >  7:859979/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                     
CTTCTGGAAGCCGGTTACAAAGCTGATTTAACCATTTGCGATTACAACTCGCCGACGCCGCTGCTGGCAGACAATATCGCCGGGCATATCGCTTTCGGTATGGGCTCAGGCAGCGTTCACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCG  >  NC_000913/3020244‑3020773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: