Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,033,624 A→G 5.0% intergenic (+11/+33) idi → / ← lysS isopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6240AG 5.0% 724.3 / 16.5 215intergenic (+11/+33)idi/lysSisopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (99/105);  new base G (7/4);  total (106/109)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 217 total aligned reads displayed.

ACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCC  >  NC_000913/3033366‑3033876
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            
aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                                                                                                                                                                                                                              >  2:124885/1‑275 (MQ=255)
aCTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGAGCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCAAGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                                                                                                                                                                                                                              <  2:260205/275‑1 (MQ=255)
                             ggCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCAACGCCGTGGNCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAACCGATTATCTGCATTTACCCAGCTTAAATAANAAAACCCCGAAATTTGCCGGGGGTGNGGGCATAACGTAATGCTTATTTTTCCGGa                                                                                                                                                                                                                 >  3:62739/1‑275 (MQ=255)
                                                                  cacgcacCACTAGTGCTTTACAGTTCAATGATGATAAAGGGATGGTGTATATATGGTGGTATGTAGCAGATGGATTAAACGGGATTGATGCCACGCCGTGGGCGGTCAGTCCGTGGATGGTGATGCAGGAGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt                                                                                                                                                                            <  4:275388/275‑1 (MQ=255)
                                                                   acgcacCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                                                                                                                            >  4:187461/1‑242 (MQ=255)
                                                                       accacTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc                                                                                                                                                                                                        <  2:187461/242‑1 (MQ=255)
                                                                               tGCGTTACAGATAAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTAAACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGAGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa                                                                                                                                                               <  1:59452/275‑1 (MQ=255)
                                                                                  gTTAAAGGTCAATGATGATGAAGTGATGGATTAGCAATGGTGTGATTTAGCATGTGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg                                                                                                                                                            <  4:124885/275‑1 (MQ=255)
                                                                                         aTCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATTGCTGTTGGTGAACGACATTAcc                                                                                                                                                     >  1:36645/1‑275 (MQ=255)
                                                                                                           aTGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                                                                                                                          >  4:87200/1‑236 (MQ=255)
                                                                                                              gATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGa                                                                                                                                                                                                                                                              >  8:773968/1‑149 (MQ=255)
                                                                                                               aTTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt                                                                                                                                                                      <  2:87200/236‑1 (MQ=255)
                                                                                                                ttATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACa                                                                                                                                                                                                                                                            >  6:20602/1‑149 (MQ=255)
                                                                                                                  aTCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAtt                                                                                                                                                                                                                                                          <  6:1176978/149‑1 (MQ=255)
                                                                                                                   tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAttt                                                                                                                                                                                                                                                         >  5:124055/1‑149 (MQ=255)
                                                                                                                    cAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCaaatg                                                                                                                                                                                                                                                        >  6:58441/1‑145 (MQ=255)
                                                                                                                    cAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                                                                                                                                        >  6:1410876/1‑149 (MQ=255)
                                                                                                                        ggTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg                                                                                                                      >  4:36723/1‑275 (MQ=255)
                                                                                                                        ggTGAGATTTAGCAGATGTATTACACGGTATTGACGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTCTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCga                                                                                                                                                                                                                                                    <  8:433703/149‑2 (MQ=255)
                                                                                                                         gtgtGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCggg                                                                                                                                                                                                                                                   <  7:773968/149‑1 (MQ=255)
                                                                                                                         gtgtGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCggg                                                                                                                                                                                                                                                   <  5:25050/149‑1 (MQ=255)
                                                                                                                          tgtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCgggg                                                                                                                                                                                                                                                  >  5:187927/1‑149 (MQ=255)
                                                                                                                           gtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGt                                                                                                                                                                                                                                                 <  6:1243256/149‑1 (MQ=255)
                                                                                                                              aTTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAGAACCCCGACATTTGCCGGGGTtgt                                                                                                                                                                                                                                              <  6:401303/149‑1 (MQ=255)
                                                                                                                              aTTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                                                                                                                                                                                                                              <  8:791137/149‑1 (MQ=255)
                                                                                                                                ttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGa                                                                                                                                                                                                                                            <  5:51367/149‑1 (MQ=255)
                                                                                                                                ttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGa                                                                                                                                                                                                                                            <  7:1183248/149‑1 (MQ=255)
                                                                                                                                     aGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATa                                                                                                                                                                                                                                       >  6:1455853/1‑149 (MQ=255)
                                                                                                                                      gATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCTGTCCGTGGATGGTGATGCAGGCTACAAATCGCGACGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAACACCCCGACATTTGCCGGGGTTGAGAGCATaa                                                                                                                                                                                                                                      >  7:902503/1‑149 (MQ=255)
                                                                                                                                       aTGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAAc                                                                                                                                                                                                                                     <  5:745549/149‑1 (MQ=255)
                                                                                                                                           aTTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTaa                                                                                                                                                                                                                                 <  5:849418/149‑1 (MQ=255)
                                                                                                                                              acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                                                                                                                              <  5:1410876/149‑1 (MQ=255)
                                                                                                                                               cacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                                                                                                                             <  6:697733/149‑1 (MQ=255)
                                                                                                                                               cacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGAGGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                                                                                                                             >  8:842198/1‑149 (MQ=255)
                                                                                                                                                  ggTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAt                                                                                                                                                                                                                          >  6:1344585/1‑149 (MQ=255)
                                                                                                                                                  ggTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCCGATGGTATGGCTGTTGGTGAACAGCATTACCATCCGGTCGATACCAATTCCCAGACCTGCTCGCGGCGGTAAGCCATTGTCCAGttcc                                                                                            >  4:338288/1‑272 (MQ=255)
                                                                                                                                                  ggTATTGATGCCACGCCGTGGGCGTGCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAt                                                                                                                                                                                                                          <  5:21854/149‑1 (MQ=255)
                                                                                                                                                    tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGACGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgtg                                                                                                                                                                                                                                             >  2:365417/1‑128 (MQ=255)
                                                                                                                                                      ttGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCACAAAACTTCTGTCGGCGGTAAGCCATGTTCCAGTCCGGttc                                                                                        >  1:12485/1‑273 (MQ=255)
                                                                                                                                                         aTGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCg                                                                                                                                                                                                                   >  8:342126/1‑149 (MQ=255)
                                                                                                                                                          tGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg                                                                                                                                                            <  4:229925/203‑1 (MQ=255)
                                                                                                                                                            ccACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGAc                                                                                                                                                                                                                <  6:480601/149‑1 (MQ=255)
                                                                                                                                                             cACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACg                                                                                                                                                                                                               >  7:440778/1‑149 (MQ=255)
                                                                                                                                                              aCGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGc                                                                                                                                                                                                              >  5:1143206/1‑149 (MQ=255)
                                                                                                                                                              aCGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCCCGGAAGGTATGGCAGTTGGGTAACAGCATTACCAGACGGTCGTAACCAAGTCCCAGACCTGCCGTCAGCGGGAAGCCACGTTCCAGTGCgcgtagctaa                                                                                   >  4:10365/1‑263 (MQ=255)
                                                                                                                                                                gCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                                                                                                                            >  7:506107/1‑149 (MQ=255)
                                                                                                                                                                      ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCgg                                                                                                                                                                                                      <  5:295408/149‑1 (MQ=255)
                                                                                                                                                                        cGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa                                                                                                                                                                                                    <  8:255103/149‑1 (MQ=255)
                                                                                                                                                                           tCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACa                                                                                                                                                                                                 <  7:342126/149‑1 (MQ=255)
                                                                                                                                                                                ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATa                                                                                                                                                                                            >  5:1420944/1‑149 (MQ=255)
                                                                                                                                                                                  gTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAAc                                                                                                                                                                                          <  7:969550/149‑1 (MQ=255)
                                                                                                                                                                                    ggATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGt                                                                                                                                                                                        >  7:1363425/1‑149 (MQ=255)
                                                                                                                                                                                       tGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc                                                                                                                                                                                     <  8:311402/149‑1 (MQ=255)
                                                                                                                                                                                       tGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                                                                                                 <  2:373224/201‑1 (MQ=255)
                                                                                                                                                                                           gATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGAt                                                                                                                                                                                 <  8:1049318/149‑1 (MQ=255)
                                                                                                                                                                                                aGGCGACAAATCGCGAAGCCAGAAGACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCGTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt                                                                                                                                                                            >  8:1402822/1‑149 (MQ=255)
                                                                                                                                                                                                  gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGAc                                                                                                                                                                                                                >  6:957360/1‑111 (MQ=255)
                                                                                                                                                                                                  gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGAc                                                                                                                                                                                                                <  5:957360/111‑1 (MQ=255)
                                                                                                                                                                                                  gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                                                                                                                          <  8:14704/149‑1 (MQ=255)
                                                                                                                                                                                                    gACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCt                                                                                                                                                                        >  5:1027115/1‑149 (MQ=255)
                                                                                                                                                                                                     aCAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTa                                                                                                                                                                                                                           <  7:1240144/97‑1 (MQ=255)
                                                                                                                                                                                                     aCAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTa                                                                                                                                                                                                                           >  8:1240144/1‑97 (MQ=255)
                                                                                                                                                                                                      cAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt                                                                                                                                                                      <  7:1172043/149‑1 (MQ=255)
                                                                                                                                                                                                       aaaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                                                                                                                                     >  6:873422/1‑149 (MQ=255)
                                                                                                                                                                                                           cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg                                                                                                                                                                 <  6:282541/149‑1 (MQ=255)
                                                                                                                                                                                                              gAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATTACGTTATGCtcac                                                                                                                                                                                                                          >  8:1430933/1‑86 (MQ=255)
                                                                                                                                                                                                              gAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                                                                                                                              >  7:126622/1‑149 (MQ=255)
                                                                                                                                                                                                               aaGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                                                                                                             >  5:659342/1‑149 (MQ=255)
                                                                                                                                                                                                                    aGAAAACTATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCTGGGTTGTGAGCATATCGTAATGCTTATTTTACCGTACGCATCGCCGGGATCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                                                                                                                        <  6:1303943/149‑1 (MQ=255)
                                                                                                                                                                                                                    aGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                                                                                                                        <  5:791200/149‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                                                                                                    >  8:547645/1‑149 (MQ=255)
                                                                                                                                                                                                                                 cTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                                                                                                                           >  7:994210/1‑149 (MQ=255)
                                                                                                                                                                                                                                 cTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                                                                                                                           >  5:1404998/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                                                                                                                          >  5:1300023/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                                                                                                                          <  8:126622/149‑1 (MQ=255)
                                                                                                                                                                                                                                   gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACgg                                                                                                                                               <  5:32831/143‑1 (MQ=255)
                                                                                                                                                                                                                                   gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATa                                                                                                                                         >  5:344587/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tttACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCAGACGGTCGATACCa                                                                                                                                      >  8:924826/1‑149 (MQ=255)
                                                                                                                                                                                                                                         aCCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                                                                                                                   <  5:152246/149‑1 (MQ=255)
                                                                                                                                                                                                                                            cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                                                                                                                >  5:1446869/1‑149 (MQ=255)
                                                                                                                                                                                                                                              gCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt                                                                                                                                    <  7:711611/143‑1 (MQ=255)
                                                                                                                                                                                                                                              gCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt                                                                                                                                    >  8:711611/1‑143 (MQ=255)
                                                                                                                                                                                                                                              gCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAg                                                                                                                              >  7:820320/1‑149 (MQ=255)
                                                                                                                                                                                                                                              gCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTATTCTTCATCGTAGAACATCGCTTCGTCGTCCCCTGCGTCTTTCCCGGCCACCTGAACCAGGAAGCGTTGCGCCTGAATTTcc  >  2:39201/1‑273 (MQ=255)
                                                                                                                                                                                                                                                ttAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                                                                                                            >  5:1117211/1‑149 (MQ=255)
                                                                                                                                                                                                                                                ttAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                                                                                                            >  6:89142/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTGTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                                                                                                          <  6:423872/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                                                                                                          <  6:353488/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   aataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                                                                         >  5:354911/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                                                                                                                        <  6:320973/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     taaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                                                                                                    >  8:1182257/1‑120 (MQ=255)
                                                                                                                                                                                                                                                       tcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                                                                                                                     >  5:560711/5‑149 (MQ=255)
                                                                                                                                                                                                                                                        cacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                                                                                                             >  5:897914/4‑108 (MQ=255)
                                                                                                                                                                                                                                                        aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa                                                                                                                                                          >  6:1131645/1‑111 (MQ=255)
                                                                                                                                                                                                                                                        aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                                                                                                                    >  8:65931/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                                                                                                                    >  8:100129/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            aCCCCGGCAAATGTCGGGGTTTTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                                                                                                                          <  7:59800/123‑1 (MQ=255)
                                                                                                                                                                                                                                                            aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                                                                                <  6:1383351/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              cccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                                                                                                              >  6:772746/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa                                                                                                             >  6:1480652/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            
ACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCC  >  NC_000913/3033366‑3033876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: