BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,051,723	C→G	5.4%	D199H (GAT→CAT)	ubiI ←	2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,051,723	0	C	G	5.4%	655.7 / 22.9	187	D199H (GAT→CAT)	ubiI	2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand): ref base C (92/84); new base G (8/2); total (100/87)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

Only 100 of 195 total aligned reads displayed.

CCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCA  >  NC_000913/3051455‑3051994

cccACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGAAAGTGACCAGACAATCGAGCAAAGATCCGGATCGCTAAGCGGTACAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCCCCACCACACCAAGCgg                                                                                                                                                                                                                                                                           >  4:284610/1‑275 (MQ=255)

                    cGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCCACATGGAAAACCTGCCGCGCCCCCGCACCACGCGGTTCTTCCGTGCGAGTGGTCg                                                                                                                                                                                                                                                       >  4:269522/1‑275 (MQ=255)

                                                  gtcagggAACACCCGGCGGCGCCCGCCCACCCTGGCGTAAGCCCAGCGGATTATCAAAAGCTGTATTGAGCGCGAGATTAAATTCGTTTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGATAAGCGGTAACAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAg                                                                                                                                                                                                                          <  4:158444/271‑1 (MQ=255)

                                                         aaCACCTGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGCTTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGCCGACAGTGACCACACAAACGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCCGAATGCCTTCGCCATGGGAGACCTGCCGCGCAACCGCATCATGCGATTCTTCCGTCGGATTGGACGGTCCCCGCGCGTGATGCTTAGAACACCACAAAGTcgg                                                                                                                                                                                                                  >  4:131252/1‑273 (MQ=255)

                                                               tGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGACAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGCGATGCTGATAATCCCAGAAAGTCAGCGGAat                                                                                                                                                                                                            >  2:25904/1‑275 (MQ=255)

                                                                              aaCCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTgc                                                                                                                                                                                              <  2:66109/275‑1 (MQ=255)

                                                                                    gCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAAc                                                                                                                                                                                         >  2:185226/1‑274 (MQ=255)

                                                                                                            aGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCg                                                                                                                                                                                                                                                                            <  2:382295/167‑1 (MQ=255)

                                                                                                                      aaggcgcgATTAAATGCGTCTTACCCTGCCCGCTGCATCCGCGTCGGTTCCACTGGCGCCAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGAcg                                                                                                                                                      <  4:51701/272‑1 (MQ=255)

                                                                                                                       aCGCGCGATTAAATTCGTCTTCACTTGCCGGCTGAATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGAcgc                                                                                                                                                     <  2:193033/275‑1 (MQ=255)

                                                                                                                         gcgcgATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGcatc                                                                                                                                                                                                                                                                                 <  7:138567/149‑1 (MQ=255)

                                                                                                                         gaacgATTAAATTCGTCTGCGCTTCACTGCTGCAGTAGCTGCGCTTCCTTTGGCGACAGTGACCTGACAATCGAGCAAAGCTGCGTATCGCTAAGCGGTAAAAAGGCCATAATTCCTTCGCCATGGAAAACCTGCCGCGCGACCGcatc                                                                                                                                                                                                                                                                                 >  6:800962/4‑149 (MQ=255)

                                                                                                                          cgcgATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGcatca                                                                                                                                                                                                                                                                                <  8:95351/149‑1 (MQ=255)

                                                                                                                           gcgATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCAAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTACCCCCGTCCGCCCCCATCACCCTACGcccc                                                                                                                                                  >  4:188262/1‑274 (MQ=255)

                                                                                                                             gATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGc                                                                                                                                                                                                                                                                             <  7:469538/149‑1 (MQ=255)

                                                                                                                                   aTTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGttct                                                                                                                                                                                                                                                                       >  6:540860/1‑149 (MQ=255)

                                                                                                                                    ttCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGttctt                                                                                                                                                                                                                                                                      >  8:420080/1‑149 (MQ=255)

                                                                                                                                          ttCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGc                                                                                                                                                                                                                                                                >  7:112346/1‑149 (MQ=255)

                                                                                                                                             aCTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGaa                                                                                                                                                                                                                                                             >  5:524459/1‑149 (MQ=255)

                                                                                                                                             aCTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGaa                                                                                                                                                                                                                                                             <  6:299934/149‑1 (MQ=255)

                                                                                                                                                  ccTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGGGAATGGTc                                                                                                                                                                                                                                                        >  6:405465/1‑149 (MQ=255)

                                                                                                                                                      ctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGAGAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGCATTAGCGCCGTCCGACCCAATCACCCGCCGCGCCGTTAACCGGCGTCCATCttttaaccccc                                                                                                                      >  4:80453/1‑267 (MQ=255)

                                                                                                                                                           tCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTTCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGACATCTTTCAGCGTCAGGaa                                                                                                                  >  2:215719/1‑274 (MQ=255)

                                                                                                                                                             cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAgcgc                                                                                                                                                                                                                                             <  5:207634/149‑1 (MQ=255)

                                                                                                                                                              gCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGcgcg                                                                                                                                                                                                                                            >  5:1047962/1‑149 (MQ=255)

                                                                                                                                                              gCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGcgcg                                                                                                                                                                                                                                            >  5:366617/1‑149 (MQ=255)

                                                                                                                                                                tGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTg                                                                                                                                                                                                                                          >  5:1273499/1‑149 (MQ=255)

                                                                                                                                                                 gcgcTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGa                                                                                                                                                                                                                                         <  5:983794/149‑1 (MQ=255)

                                                                                                                                                                  cgcTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGAt                                                                                                                                                                                                                                        <  5:334967/149‑1 (MQ=255)

                                                                                                                                                                   gcTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATg                                                                                                                                                                                                                                       >  7:248480/1‑149 (MQ=255)

                                                                                                                                                                       ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTg                                                                                                                                                                                                                                                                 >  1:20714/1‑119 (MQ=255)

                                                                                                                                                                       ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGa                                                                                                                                                                                                                                   <  7:1250484/149‑1 (MQ=255)

                                                                                                                                                                       ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGa                                                                                                                                                                                                                                   <  6:1461404/149‑1 (MQ=255)

                                                                                                                                                                        ctctGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGAt                                                                                                                                                                                                                                  >  8:1199204/1‑149 (MQ=255)

                                                                                                                                                                        ctctGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGAt                                                                                                                                                                                                                                  <  7:823371/149‑1 (MQ=255)

                                                                                                                                                                         tctGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATa                                                                                                                                                                                                                                 >  8:21101/1‑149 (MQ=255)

                                                                                                                                                                         tctGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATa                                                                                                                                                                                                                                 >  5:482602/1‑149 (MQ=255)

                                                                                                                                                                         tctGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGCCGCTACCAGCGCGTGATGCTGATa                                                                                                                                                                                                                                 >  7:961839/1‑149 (MQ=255)

                                                                                                                                                                           tGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGaa                                                                                                                                                                                                                                                             <  3:20714/119‑1 (MQ=255)

                                                                                                                                                                            ggCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATc                                                                                                                                                                                                                              <  5:475280/149‑1 (MQ=255)

                                                                                                                                                                               gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCa                                                                                                                                                                                                                           <  6:47931/149‑1 (MQ=255)

                                                                                                                                                                               gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCa                                                                                                                                                                                                                           >  6:1294739/1‑149 (MQ=255)

                                                                                                                                                                                 cAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGa                                                                                           <  2:80453/275‑1 (MQ=255)

                                                                                                                                                                                  aGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGaa                                                                                                                                                                                                                        >  6:1455891/1‑149 (MQ=255)

                                                                                                                                                                                  aGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGGGCCGTCCGCTCCCATCACCAGACGCGCCGTTAACATGCTGCCACCTTTCAGCGTCAGGCAGGTTTCTTTTTCTCCCGCGGCGa                                                                                           >  1:60454/1‑274 (MQ=255)

                                                                                                                                                                                   gggACCCGACAATCGAGAAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGGATGCCTTCGCCATGGAAAACATTCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGAcc                                                                                         <  2:278235/273‑1 (MQ=255)

                                                                                                                                                                                   gTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGaaa                                                                                                                                                                                                                       <  6:711152/149‑1 (MQ=255)

                                                                                                                                                                                     gACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCa                                                                                                                                                                                                                           >  5:864827/1‑143 (MQ=255)

                                                                                                                                                                                     gACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCa                                                                                                                                                                                                                           <  6:864827/143‑1 (MQ=255)

                                                                                                                                                                                     gACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGaaa                                                                                                                                                                                                                       >  8:180217/1‑147 (MQ=255)

                                                                                                                                                                                     gACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGaaa                                                                                                                                                                                                                       <  7:180217/147‑1 (MQ=255)

                                                                                                                                                                                         aGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATc                                                                                                                                                                                                                              >  8:704141/1‑136 (MQ=255)

                                                                                                                                                                                         aGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATc                                                                                                                                                                                                                              <  7:704141/136‑1 (MQ=255)

                                                                                                                                                                                         aGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGc                                                                                                                                                                                                                 <  7:216292/149‑1 (MQ=255)

                                                                                                                                                                                          gACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCg                                                                                                                                                                                                                <  7:603437/149‑1 (MQ=255)

                                                                                                                                                                                           aCAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAg                                                                                                                                                                                                                                                >  1:23589/1‑116 (MQ=255)

                                                                                                                                                                                             aaTCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGaa                                                                                                                                                                                                             <  5:1455891/149‑1 (MQ=255)

                                                                                                                                                                                                   ggaagaaTGCGGATCGGTAAGGCGTAGAAAGGCCGGAATGCAGTCGACATGGAAGTACTCGCACTCAACCGCAGTATGCAGTGCTTCCGTGCGAATGGTCGCTACCAGTGCGTGATGCTGATAATCCCAGAAAGTCCGCGGAATATCGGCTTTGTTGCGCAACCAGGAATGAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTGCTCCCCAGGCGACCTGGTGTAATTCTGCg                                                                          <  4:255705/268‑1 (MQ=255)

                                                                                                                                                                                                   gCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCgg                                                                                                                                                                                                       >  7:675763/1‑149 (MQ=255)

                                                                                                                                                                                                    cAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGc                                                                                                                                                                                                      >  5:1336512/1‑149 (MQ=255)

                                                                                                                                                                                                      aaGATGCGGATCGCTAAGCGGGAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCtt                                                                                                                                                                                                    >  8:256451/1‑149 (MQ=255)

                                                                                                                                                                                                           gCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTttgttg                                                                                                                                                                                               <  6:482602/149‑1 (MQ=255)

                                                                                                                                                                                                            cGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTgc                                                                                                                                                                                              <  6:588504/149‑1 (MQ=255)

                                                                                                                                                                                                            cGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTgc                                                                                                                                                                                              <  5:540860/149‑1 (MQ=255)

                                                                                                                                                                                                            cGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTgc                                                                                                                                                                                              <  7:635762/149‑1 (MQ=255)

                                                                                                                                                                                                            cGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTgc                                                                                                                                                                                              <  6:1336512/149‑1 (MQ=255)

                                                                                                                                                                                                            cGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACa                                                                <  2:144030/275‑1 (MQ=255)

                                                                                                                                                                                                                   cTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCa                                                                                                                                                                                       <  8:675763/149‑1 (MQ=255)

                                                                                                                                                                                                                   cTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCa                                                                                                                                                                                       <  8:1343959/149‑1 (MQ=255)

                                                                                                                                                                                                                    tAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAg                                                                                                                                                                                      >  7:576721/1‑149 (MQ=255)

                                                                                                                                                                                                                    tAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAg                                                                                                                                                                                      >  6:1367390/1‑149 (MQ=255)

                                                                                                                                                                                                                    tAAGCGGTAAAAAGGCCAGAATGCCTTCGACATGGGAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAGTATCGGCTTTGTTGCGCAACCAg                                                                                                                                                                                      >  5:800150/1‑149 (MQ=255)

                                                                                                                                                                                                                       gCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGaa                                                                                                                                                                                   <  5:951346/149‑1 (MQ=255)

                                                                                                                                                                                                                       gCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGaa                                                                                                                                                                                   <  5:1103479/149‑1 (MQ=255)

                                                                                                                                                                                                                          gTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTc                                                                                                                                                                                                                                                        >  5:1133178/1‑77 (MQ=255)

                                                                                                                                                                                                                          gTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTc                                                                                                                                                                                                                                                        <  6:1133178/77‑1 (MQ=255)

                                                                                                                                                                                                                          gTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAg                                                                                                                                                                                      <  7:352550/143‑1 (MQ=255)

                                                                                                                                                                                                                                  gCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCACCCAGGAATTAGCGCCGTCCGCGCCCATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATGTTCTCCCCAGGCGACCTGCGGTCATTCTGCGGGGGCTAACAGAGTGATATTTAACACCTGAtc                                          >  1:62574/1‑274 (MQ=255)

                                                                                                                                                                                                                                     aGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGaa                                                                                                                                                                                                             <  8:1094027/109‑1 (MQ=255)

                                                                                                                                                                                                                                     aGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCcgc                                                                                                                                                                     >  8:378041/1‑149 (MQ=255)

                                                                                                                                                                                                                                       aaTGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCgcgc                                                                                                                                                                   >  6:1116570/1‑149 (MQ=255)

                                                                                                                                                                                                                                          gCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCaa                                                                                                                                                                >  5:1154421/1‑149 (MQ=255)

                                                                                                                                                                                                                                            cTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATc                                                                                                                                                              >  7:1452719/1‑149 (MQ=255)

                                                                                                                                                                                                                                                  cATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGa                                                                                                                                                        <  7:1357589/149‑1 (MQ=255)

                                                                                                                                                                                                                                                       aaaaCCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACgcgc                                                                                                                                                   >  6:1240784/1‑149 (MQ=255)

                                                                                                                                                                                                                                                          aCCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAAt                                                                                                                                                                                                                                                            >  8:16121/1‑41 (MQ=255)

                                                                                                                                                                                                                                                            cTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTa                                                                                                                                              >  8:4892/1‑149 (MQ=255)

                                                                                                                                                                                                                                                             tGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTaa                                                                                                                                             <  7:161983/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              gCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAAc                                                                                                                                            >  7:1269346/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               ccGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGCGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCCCGTAGTGAATcc             >  2:148877/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                ggaagaaCCGCATGATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACAt                                                                                                                                          >  5:188328/7‑149 (MQ=255)

                                                                                                                                                                                                                                                                    gaaCCGCATGATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACAtgctg                                                                                                                                      >  5:627143/3‑149 (MQ=255)

                                                                                                                                                                                                                                                                     cACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACAtgctgc                                                                                                                                     <  6:1190343/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                     aaCCGCATGATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCANACGCGCCGTTAACATGCTGCCATCCTTCACCGTCCGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGNACTTCTGCGGGGGCTCACAGAGTGATATCTGACGCCTGATGCACANTNTTTCCAAGCGCGTAGTCaaaaactcaa        >  3:39097/2‑266 (MQ=255)

                                                                                                                                                                                                                                                                     aaCCGCATGATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACAtgctgc                                                                                                                                     >  8:632850/2‑149 (MQ=255)

                                                                                                                                                                                                                                                                     aaCCGCATGATGCGGTTCTTCCGCGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGtt                                                                                                                                               <  8:307502/138‑1 (MQ=255)

                                                                                                                                                                                                                                                                     aaCCGCATGATGCGGTTCTTCCGCGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGtt                                                                                                                                               >  7:307502/2‑139 (MQ=255)

                                                                                                                                                                                                                                                                      aCCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGcc                                                                                                                                    <  5:646014/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                          catcctGCGGTTCTTCCATGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCa  <  2:342827/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                             catGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTc                                                                                                                             <  6:280565/149‑1 (MQ=255)

CCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAC‑GCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCCACCGCATCATGCGGTTCTTCCGTGCGAATGGTCGCTACCAGCGCGTGATGCTGATAATCCCAGAAAGTCAGCGGAATATCGGCTTTGTTGCGCAACCAGGAATTAGCGCCGTCCGCGCCAATCACCAGACGCGCCGTTAACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCA  >  NC_000913/3051455‑3051994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`