Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,171,858 | A→T | 5.9% | intergenic (+25/+21) | qseC → / ← ygiZ | quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,171,858 | 0 | A | T | 5.9% | 530.8 / 18.0 | 152 | intergenic (+25/+21) | qseC/ygiZ | quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (69/74); new base T (6/3); total (75/77) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01 |
Only 100 of 164 total aligned reads displayed.
CACAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAA > NC_000913/3171589‑3172127 | cccagggcagggTGGTATACGTCACGCGTAATGCTGAGAATTTCAGCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑Ga < 4:19401/273‑1 (MQ=255) aaaggtgagggtgtTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGGCACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑Ga < 4:252999/264‑1 (MQ=255) cggggCAGGGTGGGCGCCGTAACGCTGNNTGCTGATGATTTCATCGTGGGGGATAACGGNCCCGGGGGGAAACCAGAGGCCCTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGNGGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑Ga < 3:43234/271‑1 (MQ=255) aGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTTCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCCATGTCTATGAGAGCCGTTaaa > 2:252999/1‑268 (MQ=255) cGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTTGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTTAGCTGGTAACTTCTCTGTCTATTGGAGCCGTTAAAAC‑GACTCTCAAAGa > 2:282236/1‑275 (MQ=255) tGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa > 4:219231/1‑240 (MQ=255) ccGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCCCCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGAAGGGCATGACAGTTTAATTTGGGAATGCGGAAAAAGGTGGATTTGAGGCGAAGGGAAGCTGGTAACTTCTCGGTCTATGAGAGCCTTTAAAAA‑GACCCCCATTGGTTTTGCCAATGACCAAAAATAACACGGCACCAAAAAAGTCAACACACCACaac > 4:19621/1‑274 (MQ=255) tggccagAATTGGCGCACGCTTCTATCGCCCACCCGGACAAATCGCTACCGGCATCGGGCGTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAAAAAGGTGGATTTGAGGCGAAGGTAATCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGaa < 2:46002/269‑1 (MQ=255) gAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GAct > 2:354930/1‑187 (MQ=255) tGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCa < 4:354930/187‑1 (MQ=255) aaaCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa > 8:1124869/1‑149 (MQ=255) aaaCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa > 7:237736/1‑149 (MQ=255) aaaCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTACATGGCATGAATGTTGGATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa < 5:426496/149‑1 (MQ=255) aCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑g > 7:121235/1‑149 (MQ=255) cGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa > 7:1146896/1‑145 (MQ=255) gCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GAct > 8:405145/1‑149 (MQ=255) tACCGGCAGCGGGCTTTGGGTATTGATGGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt < 4:282236/275‑1 (MQ=255) ccGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCa < 6:498170/149‑1 (MQ=255) cggCGGGCTTGGGCTATCGATTGTGCAGCGAATCGCCAAACTGCATGGCAGTAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata < 2:19621/273‑1 (MQ=255) aGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAAC‑GGCTCTCATAGac < 6:357834/149‑2 (MQ=255) ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTa < 8:1357197/149‑1 (MQ=255) gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTAc < 7:1124869/149‑1 (MQ=255) gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTAc > 6:1040150/1‑149 (MQ=255) gggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAAt < 8:121235/149‑1 (MQ=255) ggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATa > 7:1310439/1‑149 (MQ=255) gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAat > 8:1165767/1‑149 (MQ=255) tttGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCGGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAata < 8:630730/148‑1 (MQ=255) aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATa < 7:1245807/128‑1 (MQ=255) aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAata < 7:854270/149‑1 (MQ=255) aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAata > 7:343630/1‑149 (MQ=255) tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAAtata < 7:741688/149‑1 (MQ=255) tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATaaa > 5:115969/1‑149 (MQ=255) aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCg > 7:3277/1‑149 (MQ=255) cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc < 7:839430/149‑1 (MQ=255) gAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAACAGCAAAATATAAACCGtac < 6:1016192/149‑3 (MQ=255) ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa > 7:506746/1‑149 (MQ=255) ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa > 7:167260/1‑149 (MQ=255) aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTa < 6:1201778/117‑1 (MQ=255) aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTa > 5:1201778/1‑117 (MQ=255) aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa < 7:72525/149‑1 (MQ=255) aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa < 7:1006299/149‑1 (MQ=255) aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc < 5:54697/149‑1 (MQ=255) aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc < 5:1467250/149‑1 (MQ=255) aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc > 8:1010796/1‑149 (MQ=255) tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca < 5:1355581/149‑1 (MQ=255) catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc > 6:378138/1‑149 (MQ=255) atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacca > 5:642113/1‑149 (MQ=255) atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacca > 7:1363598/1‑149 (MQ=255) ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCacca‑ac < 6:371422/149‑1 (MQ=255) gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAAC‑GGCTCTCATAGacagag > 8:884795/1‑98 (MQ=37) gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACaa > 8:1253960/1‑149 (MQ=255) aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACaaa < 8:194141/149‑1 (MQ=255) aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAAc < 6:281000/149‑1 (MQ=255) tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAAcc > 5:593630/1‑149 (MQ=255) gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCa < 8:686900/149‑1 (MQ=255) ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa < 6:105949/129‑1 (MQ=255) ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa > 5:105949/1‑129 (MQ=255) tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGa < 7:459381/149‑1 (MQ=255) tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACaaa > 7:823001/1‑149 (MQ=255) aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcga < 7:1078139/149‑1 (MQ=255) aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcga < 5:1221124/149‑1 (MQ=255) aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcgag > 5:716844/1‑149 (MQ=255) gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcgagcg > 7:1447863/1‑149 (MQ=255) gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGAAACTTCCCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAAGATAAACCGTCCCCAAAAAAGCCACCA‑ACCCCAAACCAGACAAATCCCAAGGgaga > 8:1032379/1‑149 (MQ=255) aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAATAAAGCCACCA‑ACCACGAACCAGACAAAGACCGAGCGAGAAt < 6:567344/149‑1 (MQ=255) aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACa < 7:606676/149‑1 (MQ=255) ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAAt > 3:27135/1‑80 (MQ=255) gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACata > 5:275361/1‑149 (MQ=255) tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcgagc > 7:374408/1‑137 (MQ=255) tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACcgagc < 8:374408/137‑1 (MQ=255) gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCa < 1:27135/80‑1 (MQ=255) gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATAc < 8:823001/149‑1 (MQ=255) tttGAGGCGAAGGTAAGCCGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATAccc > 6:164565/1‑149 (MQ=255) aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccaca > 1:52923/1‑102 (MQ=255) gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt > 7:799443/1‑149 (MQ=255) aGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGaa < 8:1346725/149‑1 (MQ=255) aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGaa < 7:1253960/149‑1 (MQ=255) aGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt < 6:593630/149‑1 (MQ=255) gTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGc > 7:239644/1‑149 (MQ=255) ttCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa > 8:1072079/1‑149 (MQ=255) ttCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa > 6:800954/1‑149 (MQ=255) ttCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa > 6:1132466/1‑149 (MQ=255) taaaatCTATGAGAGTCGTTTTAAC‑GGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga > 7:432898/6‑149 (MQ=39) ctctGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga > 6:1441045/1‑149 (MQ=255) tGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtt < 7:1072079/149‑1 (MQ=255) gTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAata > 7:1023412/1‑52 (MQ=255) tCTATGAGAGTCGTTTTAAC‑GGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt > 7:812668/1‑149 (MQ=255) tCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt < 8:837425/149‑1 (MQ=255) tATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc < 8:608024/148‑1 (MQ=255) aTGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt > 5:1300220/1‑149 (MQ=255) tGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta < 6:115969/149‑1 (MQ=255) gagaGTCGTTTTAAC‑GGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa < 8:244688/149‑1 (MQ=255) agCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTACCGc < 8:232685/129‑1 (MQ=255) ccGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAg > 2:125327/1‑255 (MQ=255) cGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataag < 5:378138/149‑1 (MQ=255) cttAAAACAGACACTCATAAAATATACTAATAGCAAAAAAAAAACCGTCCCCAAAAAAACCACCA‑ACCACAAACCAGNAAAAAACCGAACGAGAATACATATACCCAAAACTCGAACAATTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGaa < 3:69507/274‑1 (MQ=255) ttAAAAC‑GACTCTCATCGTTTTTTCTGCTAGCAAACTATACACCGTCCCCAAACACGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGGATACATATACCCAAACCTCGACCATTTCCCGCATAACGAGTTTTCTtaagataagca > 6:1314672/1‑147 (MQ=255) ttAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaa > 8:49597/1‑149 (MQ=255) ttAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaa < 5:3413/149‑1 (MQ=255) aaaaC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaata > 5:224758/1‑149 (MQ=255) | CACAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAC‑GACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCA‑ACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAA > NC_000913/3171589‑3172127 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |