Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,863 A→G 6.7% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8630AG 6.7% 470.4 / 17.3 150intergenic (+30/+16)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (71/68);  new base G (7/3);  total (78/72)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 155 total aligned reads displayed.

CAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAA  >  NC_000913/3171591‑3172127
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                         
cggggCAGGGTGGGCGCCGTAACGCTGNNTGCTGATGATTTCATCGTGGGGGATAACGGNCCCGGGGGGAAACCAGAGGCCCTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGNGGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                                                                                                                                           <  3:43234/271‑1 (MQ=255)
        cGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTTGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTTAGCTGGTAACTTCTCTGTCTATTGGAGCCGTTAAAACGACTCTCAAAGa                                                                                                                                                                                                                                                                 >  2:282236/1‑275 (MQ=255)
                                                        cGGCCCCGGTGTGACACCAGAAGCAATGGCGCGAATTGGCGAACGGTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGGTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTAT‑‑GAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                                                                                                                                                                                              <  2:57060/275‑1 (MQ=255)
                                                             ccGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCCCCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGAAGGGCATGACAGTTTAATTTGGGAATGCGGAAAAAGGTGGATTTGAGGCGAAGGGAAGCTGGTAACTTCTCGGTCTATGAGAGCCTTTAAAAAGACCCCCATTGGTTTTGCCAATGACCAAAAATAACACGGCACCAAAAAAGTCAACACACCACaac                                                                                                                                                                                                            >  4:19621/1‑274 (MQ=255)
                                                                                  tggccagAATTGGCGCACGCTTCTATCGCCCACCCGGACAAATCGCTACCGGCATCGGGCGTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAAAAAGGTGGATTTGAGGCGAAGGTAATCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                                                                                                                                      <  2:46002/269‑1 (MQ=255)
                                                                                       cgAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                                                                                                                                           <  4:272046/186‑1 (MQ=255)
                                                                                        gAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                                                                                                                                                                                                         >  2:354930/1‑187 (MQ=255)
                                                                                            tGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                                                                                                                                                                                                     <  4:354930/187‑1 (MQ=255)
                                                                                                               gcagccGGACAAACCGGCACCTGCCGCTGGCTTGGGTGATTGGTTGTGCAGGCAATCGCCAAATTGCATGGGCTCAATGGTGAATTTGGGAATTCGGAACAAGGTGGATTTGGGGCGAAGGTAAGCTGGTAACCTCTTTGTCTATGAGAGCCGTTAATACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAATGCCACCAACCGCTAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                                                                                                                                          <  4:208107/270‑1 (MQ=255)
                                                                                                                              gCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                                                                                                                                                                                                         >  8:405145/1‑149 (MQ=255)
                                                                                                                                tACCGGCAGCGGGCTTTGGGTATTGATGGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                                                                                                                                        <  4:282236/275‑1 (MQ=255)
                                                                                                                                  ccGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                                                                                                                                                                                                     <  6:498170/149‑1 (MQ=255)
                                                                                                                                      cggCGGGCTTGGGCTATCGATTGTGCAGCGAATCGCCAAACTGCATGGCAGTAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                                                                                                                                  <  2:19621/273‑1 (MQ=255)
                                                                                                                                           ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                                                                                                                                                            <  8:1357197/149‑1 (MQ=255)
                                                                                                                                            gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                                                                                                                                                           <  7:1124869/149‑1 (MQ=255)
                                                                                                                                               tGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                                                                                                                                        <  7:1438039/149‑1 (MQ=255)
                                                                                                                                                gggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                                                                                                                                                       <  8:121235/149‑1 (MQ=255)
                                                                                                                                                 ggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                                                                                                                                                                                                      >  7:1310439/1‑149 (MQ=255)
                                                                                                                                                    tATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                                                                                                                                   >  6:1189521/1‑149 (MQ=255)
                                                                                                                                                     aTCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                                                                                                                                  >  6:1355581/1‑149 (MQ=255)
                                                                                                                                                     aTCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                                                                                                                                  >  6:1467250/1‑149 (MQ=255)
                                                                                                                                                        gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                                                                                                                                                                                                               >  8:1288/1‑149 (MQ=255)
                                                                                                                                                        gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                                                                                                                                                                                                               >  8:1165767/1‑149 (MQ=255)
                                                                                                                                                         aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                                                                                                                                                                                                   <  7:1245807/128‑1 (MQ=255)
                                                                                                                                                         aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                                                                                                                                              >  7:343630/1‑149 (MQ=255)
                                                                                                                                                           tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAAtata                                                                                                                                                                                                                                            <  7:741688/149‑1 (MQ=255)
                                                                                                                                                             tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                                                                                                                                          >  5:115969/1‑149 (MQ=255)
                                                                                                                                                                aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                                                                                                                                                                                                       >  7:1253875/1‑149 (MQ=255)
                                                                                                                                                                aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                                                                                                                                                                                                       >  7:3277/1‑149 (MQ=255)
                                                                                                                                                                  cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc                                                                                                                                                                                                                                     <  7:839430/149‑1 (MQ=255)
                                                                                                                                                                   gAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAACAGCAAAATATAAACCGtac                                                                                                                                                                                                                                    <  6:1016192/149‑3 (MQ=255)
                                                                                                                                                                    aaTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                                                                                                                                                                                                   <  7:439964/149‑1 (MQ=255)
                                                                                                                                                                        gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaa                                                                                                                                                                                                                               >  6:38694/1‑149 (MQ=255)
                                                                                                                                                                         ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa                                                                                                                                                                                                                              >  7:506746/1‑149 (MQ=255)
                                                                                                                                                                         ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa                                                                                                                                                                                                                              >  7:167260/1‑149 (MQ=255)
                                                                                                                                                                           aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                                                                                                                                                            <  6:1201778/117‑1 (MQ=255)
                                                                                                                                                                           aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                                                                                                                                            >  6:105297/1‑149 (MQ=255)
                                                                                                                                                                           aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                                                                                                                                            <  5:694300/149‑1 (MQ=255)
                                                                                                                                                                           aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                                                                                                                                            <  7:1006299/149‑1 (MQ=255)
                                                                                                                                                                               tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                                                                                                                                                                                                        <  5:1355581/149‑1 (MQ=255)
                                                                                                                                                                               tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                                                                                                                                                                                                        <  8:237736/149‑1 (MQ=255)
                                                                                                                                                                                  atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacca                                                                                                                                                                                                                     >  7:1363598/1‑149 (MQ=255)
                                                                                                                                                                                    ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                                                                                                                                                  <  6:371422/149‑1 (MQ=255)
                                                                                                                                                                                         gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacagag                                                                                                                                                                                                                                                            >  8:884795/1‑98 (MQ=37)
                                                                                                                                                                                         gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaa                                                                                                                                                                                                             <  7:968055/149‑1 (MQ=255)
                                                                                                                                                                                         gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaa                                                                                                                                                                                                             >  8:1253960/1‑149 (MQ=255)
                                                                                                                                                                                           aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                                                                                                                                           <  6:281000/149‑1 (MQ=255)
                                                                                                                                                                                            tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                                                                                                                                                                                                          >  5:593630/1‑149 (MQ=255)
                                                                                                                                                                                             gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCa                                                                                                                                                                                                         <  8:686900/149‑1 (MQ=255)
                                                                                                                                                                                             gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCa                                                                                                                                                                                                         <  5:1327931/149‑1 (MQ=255)
                                                                                                                                                                                              ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa                                                                                                                                                                                                                             >  5:105949/1‑129 (MQ=255)
                                                                                                                                                                                              ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa                                                                                                                                                                                                                             <  6:105949/129‑1 (MQ=255)
                                                                                                                                                                                                         aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                                                                                                                                                                                             <  7:1078139/149‑1 (MQ=255)
                                                                                                                                                                                                         aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                                                                                                                                                                                             <  5:1221124/149‑1 (MQ=255)
                                                                                                                                                                                                               gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGAAACTTCCCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAAGATAAACCGTCCCCAAAAAAGCCACCAACCCCAAACCAGACAAATCCCAAGGgaga                                                                                                                                                                                       >  8:1032379/1‑149 (MQ=255)
                                                                                                                                                                                                                 aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAATAAAGCCACCAACCACGAACCAGACAAAGACCGAGCGAGAAt                                                                                                                                                                                     <  6:567344/149‑1 (MQ=255)
                                                                                                                                                                                                                  cAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATa                                                                                                                                                                                    >  7:800730/1‑149 (MQ=255)
                                                                                                                                                                                                                    aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa                                                                                                                                                                                  <  7:606676/149‑1 (MQ=255)
                                                                                                                                                                                                                      gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                                                                                                                                                >  5:275361/1‑149 (MQ=255)
                                                                                                                                                                                                                       tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                                                                                                                                           >  7:374408/1‑137 (MQ=255)
                                                                                                                                                                                                                         gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                                                                                                                                   <  1:27135/80‑1 (MQ=255)
                                                                                                                                                                                                                         gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc                                                                                                                                                                             <  8:823001/149‑1 (MQ=255)
                                                                                                                                                                                                                          aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                                                                                                                                                            >  5:761543/1‑149 (MQ=255)
                                                                                                                                                                                                                           tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAccc                                                                                                                                                                           >  8:1398604/1‑149 (MQ=255)
                                                                                                                                                                                                                               aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccaca                                                                                                                                                                                                                      >  1:52923/1‑102 (MQ=255)
                                                                                                                                                                                                                               aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCaaaa                                                                                                                                                                       >  5:187741/1‑149 (MQ=255)
                                                                                                                                                                                                                                   gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                                                                                                                                                                                                 <  3:52923/103‑1 (MQ=255)
                                                                                                                                                                                                                                   gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg                                                                                                                                                                   >  7:837425/1‑149 (MQ=255)
                                                                                                                                                                                                                                          aGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                                                                                                                                                            <  6:593630/149‑1 (MQ=255)
                                                                                                                                                                                                                                             tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTccc                                                                                                                                                         >  8:637021/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                                                                                                                                                  <  5:1189521/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                                                                                                                                                  >  6:1132466/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                                                                                                                                                  >  6:800954/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      taaaatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga                                                                                                                                                >  7:432898/6‑149 (MQ=39)
                                                                                                                                                                                                                                                      ctctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga                                                                                                                                                >  6:1441045/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtt                                                                                                                                             <  7:1072079/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                                                                                                                                              >  7:1023412/1‑52 (MQ=255)
                                                                                                                                                                                                                                                          gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                                                                                                                                              <  8:1023412/52‑1 (MQ=255)
                                                                                                                                                                                                                                                           tCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                                                                                                                                           >  7:812668/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           tCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                                                                                                                                           <  8:432898/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                                                                                                                                           <  8:837425/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt                                                                                                                                         >  7:745376/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                                                                                                                                          >  7:608024/1‑148 (MQ=255)
                                                                                                                                                                                                                                                             tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                                                                                                                                          <  8:608024/148‑1 (MQ=255)
                                                                                                                                                                                                                                                              aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                                                                                                                                        >  7:244688/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                gagaGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa                                                                                                                                      >  8:312697/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                gagaGCCGTTAAAACGTCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa                                                                                                                                      <  7:1141776/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa                                                                                                                                      >  5:559847/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa                                                                                                                                      <  8:506746/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGGCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaag                                                                                                                                     <  6:1100204/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga                                                                                                                                    >  6:3413/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTACCGc                                                                                                                                                       >  7:232685/1‑129 (MQ=255)
                                                                                                                                                                                                                                                                   agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTACCGc                                                                                                                                                       <  8:232685/129‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tcgttttaaCGGCTCTAATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCTCGCATAAAGAGTTTTCTtaagataa                                                                                                                                 >  5:544849/8‑149 (MQ=255)
                                                                                                                                                                                                                                                                     ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                                                                                                                                 <  6:36534/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAg                       >  2:125327/1‑255 (MQ=255)
                                                                                                                                                                                                                                                                      cGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataag                                                                                                                                <  5:378138/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaa                                                                                                                              <  5:3413/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATc                   <  4:125327/255‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aaaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaata                                                                                                                            >  5:224758/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                         
CAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAA  >  NC_000913/3171591‑3172127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: