BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,332,822	G→A	5.3%	intergenic (‑87/+40)	yhbE ← / ← rpmA	EamA family inner membrane putative transporter/50S ribosomal subunit protein L27

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,822	0	G	A	5.3%	555.6 / 13.5	171	intergenic (‑87/+40)	yhbE/rpmA	EamA family inner membrane putative transporter/50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand): ref base G (77/84); new base A (6/3); total (84/87)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.45e-01

Only 100 of 174 total aligned reads displayed.

GCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACG  >  NC_000913/3332555‑3333089

gcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTTAGCGCCAAAAGAATGCCAATGCATTCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTCCGGATTAACCTCCGCCCCTGCCGATTTTTAACGGTTTACGCACTGAAAAACGCCCGCACCGTGTTACg                                                                                                                                                                                                                                                                         >  4:92124/1‑275 (MQ=255)

                                            gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAACAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAAGCGAAAAACTTATTCAGCTTc                                                                                                                                                                                                                             >  2:268132/1‑275 (MQ=255)

                                                          gTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTTCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGTCGCGAAAAACTTATTCAGCTTCGATGCTGATACAt                                                                                                                                                                                                                >  2:216189/1‑274 (MQ=255)

                                                                                        cttacgCACCTGCTTCGTGCCGAATGGAAACGCCCCCCAGCAAATTGAGGTGGGTGGGTCCAAAAGAAAGCCAATGCCTGCCCGCTGCTTCATACCGTAGTTCCTGCAAAGCGGATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAt                                                                                                                                                                                  <  4:92673/269‑1 (MQ=255)

                                                                                           cAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTAATCCGTTACCGGGACGAGAAAAACTTATTCAGGTTCGAGGTTGATAAATTTACGGTTTTTCGGGCCTTTTACTTTGAATTTCa                                                                                                                                                                              >  4:326963/1‑275 (MQ=255)

                                                                                                  tGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTTCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTACAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTTAGCTTCGATGATGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGACTTTCACtttcac                                                                                                                                                                       >  2:74566/1‑272 (MQ=255)

                                                                                                    cTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGt                                                                                                                                                                     <  2:34948/275‑1 (MQ=255)

                                                                                                                 ggCAACGCCCCTCAGCAAATTGCGGTGGGGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTTCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCTCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       <  2:326963/275‑1 (MQ=255)

                                                                                                                 ggCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTTGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       <  2:86080/275‑1 (MQ=255)

                                                                                                                           cccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGtt                                                                                                                                                                                                                                                                            <  7:102803/149‑1 (MQ=255)

                                                                                                                               gCAAATTGCTGTGGTGATCGCCAAAAGAATGCCAAGGCCTGCTGGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATCTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCAc                                                                                                                                         <  2:92124/275‑1 (MQ=255)

                                                                                                                               gCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCgg                                                                                                                                                                                                                                                                        <  6:1118992/149‑1 (MQ=255)

                                                                                                                                cAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCggg                                                                                                                                                                                                                                                                       <  5:375130/149‑1 (MQ=255)

                                                                                                                                  aaTTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGc                                                                                                                                                                                                                                                                     >  5:304621/1‑149 (MQ=255)

                                                                                                                                   aTTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTTCGGGGCTTTCATCCGTTACCGGGAGTCGAAAAACTTATTCCGCTTCGATGCTGGTAAATTTACGGTTTTTCGGGCCCTTAACTTCCAATTTCTCTTTACGTTCTG‑CTTTTGCAAACAGAgttgttgtccggcc                                                                                                                                     >  4:13672/1‑263 (MQ=255)

                                                                                                                                      gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTc                                                                                                                                                                                                                                                                 <  7:40533/149‑1 (MQ=255)

                                                                                                                                          tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATcc                                                                                                                                                                                                                                                             <  8:1114322/149‑1 (MQ=255)

                                                                                                                                          tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATcc                                                                                                                                                                                                                                                             >  8:491641/1‑149 (MQ=255)

                                                                                                                                          tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTAGGGACTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAACAAGAGTTTGGTCAAGACCTCAAcca                                                                                                                              >  4:298638/1‑274 (MQ=255)

                                                                                                                                            gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACAGGTTATCGAATGTAAAAAGCCCCGAAACGTGTTGCGGGGCTTTCATCCGt                                                                                                                                                                                                                                                           <  6:359136/149‑1 (MQ=255)

                                                                                                                                                     aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAc                                                                                                                                                                                                                                                  >  8:299941/1‑149 (MQ=255)

                                                                                                                                                        aGAATGCCAATGCCTGCCTGCTGCTTCATGCCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATTCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCATCGTGTTGCGGGGCTTTCATACGTTACCGGGAcgcg                                                                                                                                                                                                                                               <  8:902755/149‑1 (MQ=255)

                                                                                                                                                        aGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgt                                                                                                                                                                                                                                                                               >  7:356477/1‑117 (MQ=255)

                                                                                                                                                        aGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgt                                                                                                                                                                                                                                                                               <  8:356477/117‑1 (MQ=255)

                                                                                                                                                         aaatgcccaggccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAGTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCg                                                                                                               <  4:163578/274‑1 (MQ=255)

                                                                                                                                                            agccaacgccTGCCGGCTGCTTCCTACCGTATTCCCTGCAAAGCTCAGTGGCGGCTTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTg                                                                                                             <  1:68219/273‑1 (MQ=255)

                                                                                                                                                                aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTACCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACtt                                                                                                                                                                                                                                       <  7:656685/149‑1 (MQ=255)

                                                                                                                                                                 atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTa                                                                                                                                                                                                                                      <  7:609535/149‑1 (MQ=255)

                                                                                                                                                                  tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCAGCAACGTGTTGCGGGGCTTTCATACGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATACATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACAG‑CTGTCTTTAGCCAGCAGATTGTGGTAACGACCGAAACCTACGTTACGCCCAGCTTGGCATTTg                                                                                                      >  4:56661/1‑275 (MQ=255)

                                                                                                                                                                   gcctgcctGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAtt                                                                                                                                                                                                                                    <  7:1217929/149‑1 (MQ=255)

                                                                                                                                                                     ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCa                                                                                                                                                                                                                                  >  6:752026/1‑149 (MQ=255)

                                                                                                                                                                     ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCa                                                                                                                                                                                                                                  >  8:1362485/1‑149 (MQ=255)

                                                                                                                                                                        cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGG‑CGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑Ctt                                                                                                                                                              >  1:55035/1‑212 (MQ=255)

                                                                                                                                                                         ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCtt                                                                                                                                                                                                                              >  8:795119/1‑149 (MQ=255)

                                                                                                                                                                             tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGAt                                                                                                                                                                                                                          >  8:1299182/1‑149 (MQ=255)

                                                                                                                                                                               ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcgc                                                                                                                                                                                                                                                <  7:822258/125‑1 (MQ=255)

                                                                                                                                                                                ttCATACCTGATTCCCTGCAAAGCGCATTTGCGGATGAACGTCGCACCATGCGCAGTTTGACCGGTTATCGAATGTAAAAGGCCCCGCAACGGGTTGCCGGGCTTGCATCCGTTAGCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATGTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGac‑g                                                                                      <  4:198079/275‑1 (MQ=255)

                                                                                                                                                                                ttCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCt                                                                                                                                                                                                                       >  7:228167/1‑149 (MQ=255)

                                                                                                                                                                                  cATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGa                                                                                                                                                                                                                     <  6:248121/149‑1 (MQ=255)

                                                                                                                                                                                   aTACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGAt                                                                                                                                                                                                                    >  6:613113/1‑149 (MQ=255)

                                                                                                                                                                                    tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATa                                                                                                                                                                                                                   >  5:1296463/1‑149 (MQ=255)

                                                                                                                                                                                    tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATa                                                                                                                                                                                                                   >  8:773005/1‑149 (MQ=255)

                                                                                                                                                                                         tATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACgatga                                                                              >  2:351419/1‑274 (MQ=255)

                                                                                                                                                                                           ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc                                                                                                                                                                                                            <  8:217760/149‑1 (MQ=255)

                                                                                                                                                                                              ccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACtt                                                                                                                                                                                       >  4:351491/1‑167 (MQ=255)

                                                                                                                                                                                              ccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAg                                                                                                                                                           >  3:59117/1‑194 (MQ=255)

                                                                                                                                                                                               cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGtt                                                                                                                                                                                                        <  7:1122441/149‑1 (MQ=255)

                                                                                                                                                                                                tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCggg                                                                                                                                                                                                                                                    <  6:397033/104‑1 (MQ=255)

                                                                                                                                                                                                tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCCGGGCTTTCATCCGTTACCGGGACGCG‑AAAACTTATTCAGCTTCGATGCTGGTAAATTTACGGTTTTTCGGGCCTTGAATTTCGAATTTCCATTTACCGTCTG‑CTTTAGCAAACCGGGTGTGGTCACGCACGCAACCACACTTACCCACCGCGTGGAATCTTGT‑AACACGTTGACGGAACgatatactccca                                                                       >  4:264693/1‑265 (MQ=255)

                                                                                                                                                                                                   aaaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTc                                                                                                                                                                                                                                   <  5:497952/118‑1 (MQ=255)

                                                                                                                                                                                                   aaaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTc                                                                                                                                                                                                                                   >  6:497952/1‑118 (MQ=255)

                                                                                                                                                                                                   aaaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTc                                                                                                                                                                                                    >  6:1009483/1‑149 (MQ=255)

                                                                                                                                                                                                   aaaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTCGCAAACCGAGTGTGGTAACGACCGAAACCTACGTTAGCGCCAGGGTGGAATTTGGTCACCA‑GTTGAA‑CAACGAGTATGCTAccctc                                                                    >  4:247434/1‑272 (MQ=255)

                                                                                                                                                                                                       cgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCTCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                                                                                                                                                <  5:50297/149‑1 (MQ=255)

                                                                                                                                                                                                       cgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                                                                                                                                                <  6:1248513/149‑1 (MQ=255)

                                                                                                                                                                                                           tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                                                                                                                                                            <  5:458808/149‑1 (MQ=255)

                                                                                                                                                                                                             tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTaa                                                                                                                                                                                          <  7:49751/149‑1 (MQ=255)

                                                                                                                                                                                                              gCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAAc                                                                                                                                                                                         <  6:261328/149‑1 (MQ=255)

                                                                                                                                                                                                                ggATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTaa                                                                                                                                                                                          <  6:714613/146‑1 (MQ=255)

                                                                                                                                                                                                                ggATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTaa                                                                                                                                                                                          >  5:714613/1‑146 (MQ=255)

                                                                                                                                                                                                                 gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTc                                                                                                                                                                                      <  7:1367702/149‑1 (MQ=255)

                                                                                                                                                                                                                   ttAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGa                                                                                                                                                                                    >  7:1241577/1‑149 (MQ=255)

                                                                                                                                                                                                                       cGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAttt                                                                                                                                                                                <  5:1449176/149‑1 (MQ=255)

                                                                                                                                                                                                                        gTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTGCGAATTTc                                                                                                                                                                               <  7:1344250/149‑1 (MQ=255)

                                                                                                                                                                                                                           gCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACt                                                                                                                                                                            <  6:1455048/149‑1 (MQ=255)

                                                                                                                                                                                                                            cACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACtt                                                                                                                                                                           >  7:471806/1‑149 (MQ=255)

                                                                                                                                                                                                                                aTGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAc                                                                                                                                                                                                                                                  >  1:18536/1‑74 (MQ=255)

                                                                                                                                                                                                                                  gcgcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGt                                                                                                                                                                     >  5:905280/1‑149 (MQ=255)

                                                                                                                                                                                                                                    gcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGa                                                                                                                                                                                                                                              <  3:18536/74‑1 (MQ=255)

                                                                                                                                                                                                                                    gcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCt                                                                                                                                                                   >  7:1015856/1‑149 (MQ=255)

                                                                                                                                                                                                                                    gcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCt                                                                                                                                                                   <  5:613113/149‑1 (MQ=255)

                                                                                                                                                                                                                                    gcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCt                                                                                                                                                                   >  6:1299404/1‑149 (MQ=255)

                                                                                                                                                                                                                                        ttttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑Ctt                                                                                                                                                              <  6:893069/149‑1 (MQ=255)

                                                                                                                                                                                                                                           tACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAg                                                                                                                                                           <  5:1113588/149‑1 (MQ=255)

                                                                                                                                                                                                                                               ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTc                                                                                                                                                                    >  7:976952/1‑137 (MQ=255)

                                                                                                                                                                                                                                               ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       <  5:752026/149‑1 (MQ=255)

                                                                                                                                                                                                                                               ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       >  7:584118/1‑149 (MQ=255)

                                                                                                                                                                                                                                               ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       >  8:696362/1‑149 (MQ=255)

                                                                                                                                                                                                                                               ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCaaa                                                                                                                                                       >  6:721995/1‑149 (MQ=255)

                                                                                                                                                                                                                                                gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACGATGATGCTAACCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCt                      >  2:225596/1‑275 (MQ=255)

                                                                                                                                                                                                                                                   aTCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACaga                                                                                                                                                   <  5:1291776/149‑1 (MQ=255)

                                                                                                                                                                                                                                                   aTCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACaga                                                                                                                                                   <  5:386679/149‑1 (MQ=255)

                                                                                                                                                                                                                                                    tCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACagag                                                                                                                                                  >  8:465148/1‑149 (MQ=255)

                                                                                                                                                                                                                                                     cGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAgt                                                                                                                                                 <  7:465148/149‑1 (MQ=255)

                                                                                                                                                                                                                                                      gAATGTAAAAAGCCCGGCAACGGGTGGCGGGGCTTTTATCGGGTACCGGGACGCCAAAAACTTATTGAGCTTCGATGCTGATAAATTTACGGTTTTCCGGGCCTTTAACTTCGAGTTTCACTTTACCGTCTC‑CTTTAGCGAACAGAgtg                                                                                                                                                >  8:197247/1‑149 (MQ=255)

                                                                                                                                                                                                                                                        aTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACa                                                                                                                                                     <  6:804553/142‑1 (MQ=255)

                                                                                                                                                                                                                                                            aaaaaGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCa                                                                                                                                          <  8:1015257/149‑1 (MQ=255)

                                                                                                                                                                                                                                                             aaaaGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTc                                                                                                                                                                                                    >  7:204100/1‑91 (MQ=255)

                                                                                                                                                                                                                                                              aaaGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCt                                                                                                                                                                   >  7:845779/1‑123 (MQ=255)

                                                                                                                                                                                                                                                              aaaGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCt                                                                                                                                                                   <  8:845779/123‑1 (MQ=255)

                                                                                                                                                                                                                                                                aGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGAc                                                                                                                                      >  7:530200/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                aGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGAc                                                                                                                                      >  5:397515/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                aGCCCCGAAACGTGTTGCTGGGCTTTCAGCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGt      <  4:225596/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                 gCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGAcc                                                                                                                                     <  5:738401/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                   cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGc                                                                                                                                   >  5:851285/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGc                                                                                                                                   >  7:820041/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGc                                                                                                                                   >  5:1195605/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     cGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAg                                                                                                                                                           >  2:368127/1‑123 (MQ=255)

                                                                                                                                                                                                                                                                     cGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACCCCAAGGCCTTTAGCTTCTGAATCGTGCCCTTTAcc  >  4:30617/1‑273 (MQ=255)

                                                                                                                                                                                                                                                                      gCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAAc                                                                                                                                <  8:1178752/149‑1 (MQ=255)

GCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTG‑CTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGT‑ACCACGTTGAC‑GAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACG  >  NC_000913/3332555‑3333089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`