Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,739,717 C→T 6.0% L5F (CTT→TTT)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,739,7170CT 6.0% 2110.6 / 22.2 704L5F (CTT→TTT) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base C (411/242);  new base T (2/46);  total (413/291)
Fisher's exact test for biased strand distribution p-value = 7.88e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 724 total aligned reads displayed.

CTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAACTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACTGACCGACATGCTGGAAAGTGGCAAAGCGACTGATGCACTGTGTAACTACGACGGTCCACAGGGGAAAACGGAGCTACTCACACTGCTTGCCGGAATGCTGCGCGAGAAGTTGGGTTGGGATATCG  >  NC_000913/3739453‑3739990
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                 
          gCTACGGCTTTGTTCGTGAGCATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAACTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGCCAGATATCCCGCTATGACATTCCCCTTTTTTGGTGACAAATTTAcc                                                                                                                                                                                                                                                               >  2:309764/1‑275 (MQ=255)
                              cATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAACTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGACGCCGCCAACCAGGATAAAATGTCAGCTCTAATCAGCGTTGCAGGATAAACCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATTCAATTCTCCCTTTTTGGTGCCAAATTTACCCGCCACTCTGGCATTAcc                                                                                                                                                                                                                                             >  4:42403/1‑272 (MQ=255)
                                                     cgtcTGGGCAGGGCAACAGTAACTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTGGCACCCCAGCAAATGTAGCGTTATTGTTAACTTCCTTGCTACAGAGTTCGACAGATATCCCGATATGACATTCTCCCTTTTTGGTGACAACTATACCCGCCACTCCAGCATTACGCTGTTGATTGAAGCTCTGAacaccg                                                                                                                                                                                                                    >  1:12319/1‑271 (MQ=255)
                                                                                                                     aCCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCt                                                                                                                                                                                                                                                                                  <  5:665516/149‑1 (MQ=255)
                                                                                                                      ccAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGAACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCtt                                                                                                                                                                                                                                                                                 >  8:623835/1‑149 (MQ=255)
                                                                                                                       cAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttt                                                                                                                                                                                                                                                                                <  5:212036/149‑1 (MQ=255)
                                                                                                                        aGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCtttt                                                                                                                                                                                                                                                                               >  5:1355125/1‑149 (MQ=255)
                                                                                                                        aGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCtttt                                                                                                                                                                                                                                                                               <  7:1065358/149‑1 (MQ=255)
                                                                                                                         ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                                                                                                                                              <  6:552041/149‑1 (MQ=255)
                                                                                                                         ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                                                                                                                                              <  6:671230/149‑1 (MQ=255)
                                                                                                                          gATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTg                                                                                                                                                                                                                                                                             <  6:872314/149‑1 (MQ=255)
                                                                                                                          gATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGac                                                                                                                                                >  2:242615/1‑274 (MQ=255)
                                                                                                                           aTAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTgg                                                                                                                                                                                                                                                                            >  7:1026976/1‑149 (MQ=255)
                                                                                                                            tAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGt                                                                                                                                                                                                                                                                           <  5:630137/149‑1 (MQ=255)
                                                                                                                            tAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGt                                                                                                                                                                                                                                                                           <  6:1118821/149‑1 (MQ=255)
                                                                                                                            tAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGt                                                                                                                                                                                                                                                                           <  8:228359/149‑1 (MQ=255)
                                                                                                                               aaTGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGAc                                                                                                                                                                                                                                                                        >  7:677966/1‑149 (MQ=255)
                                                                                                                                aTGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACa                                                                                                                                                                                                                                                                       >  7:947008/1‑149 (MQ=255)
                                                                                                                                     aGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAAttt                                                                                                                                                                                                                                                                  >  5:1085700/1‑149 (MQ=255)
                                                                                                                                      gCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGa                                                                                                                                                                                                                                                                         <  7:572749/141‑1 (MQ=255)
                                                                                                                                      gCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTa                                                                                                                                                                                                                                                                 <  5:979407/149‑1 (MQ=255)
                                                                                                                                        ccTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAcc                                                                                                                                                                                                                                                               >  6:370368/1‑149 (MQ=255)
                                                                                                                                         cTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGGTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAccc                                                                                                                                                                                                                                                              >  5:1020949/1‑149 (MQ=255)
                                                                                                                                            aTCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGcc                                                                                                                                                                                                                                                           >  8:153345/1‑149 (MQ=255)
                                                                                                                                                 cGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGaccac                                                                                                                                             >  2:126560/1‑254 (MQ=255)
                                                                                                                                                        gaaTAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTa                                                                                                                                                                                                                                               <  6:141804/147‑1 (MQ=255)
                                                                                                                                                            aaaGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCt                                                                                                                                                                                                                                           >  8:1211335/1‑149 (MQ=255)
                                                                                                                                                              aGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGt                                                                                                                                                                                                                                         >  6:1480506/1‑149 (MQ=255)
                                                                                                                                                              aGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGt                                                                                                                                                                                                                                         >  8:1334629/1‑149 (MQ=255)
                                                                                                                                                               gCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTgg                                                                                                                                                                                                                                                                            >  8:1166996/1‑113 (MQ=255)
                                                                                                                                                               gCACCGCTCACTCTTCAAAAGACCGATTTCCCCCCCAGCAAATGTAGCGTTATTGTTACCTTCTTTGCTAAAGAGTTCGACGGGTATCCCGCTATCACTTCCTCCTTTTTTGGTGACAAATTTAGCCGCCACTCCGGCATTACGCTGtt                                                                                                                                                                                                                                        <  8:1253846/149‑1 (MQ=255)
                                                                                                                                                                 aCCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTTTTACCTTCCTTGCTACAGAGTTCAACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGa                                                                                                                                                                                                                                      <  7:822298/149‑1 (MQ=255)
                                                                                                                                                                  ccGCTCACTCTTCAACAGACCGATTTGCACCACAGAAAATGTAGCTTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATCCTCCTTTTTTGGTAACAAATTTACCCGCCACTCCGGCATTACGCTGTTGAt                                                                                                                                                                                                                                     <  8:579943/149‑1 (MQ=255)
                                                                                                                                                                   cgttcgctctTCAACAGACCGATTTGCCCCCCAGAAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACGGATATCCCGCTATTACATTCTCCTTTTTTGGTGAAAAATTTACCCGCCACTCTGGCATTACGCTGTTGATg                                                                                                                                                                                                                                    <  6:1146869/143‑1 (MQ=255)
                                                                                                                                                                    gCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTCCTACAGATTTCGACAGATATCCCGCTATAACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATgg                                                                                                                                                                                                                                   <  5:886195/149‑1 (MQ=255)
                                                                                                                                                                     cTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGTCGCGATTATGCTCGGCGGCGGTAATCCGGCCCAGATCCCGGAACTGCAGGACTACTTCCAGACGCTACTGACCGACCTGCTGGAAAGGTGCAATGc                                                                                                    >  2:86902/1‑275 (MQ=255)
                                                                                                                                                                         ctctTCAACAGACCGTTTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGAt                                                                                                                                                                                                                              <  6:456473/149‑1 (MQ=255)
                                                                                                                                                                                 cAGACCGATTTGCCCCCAAACAAATGTAGCTTTATTTTTACCTTCCTTGCTACAGAGTTCGACAAATACCCCGCTATGACATTCCCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAacga                                                                                                                                                                                                                      <  8:685201/149‑1 (MQ=255)
                                                                                                                                                                                  aGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAac                                                                                                                                                                                                                        <  7:145730/146‑1 (MQ=255)
                                                                                                                                                                                       gATTTGCCCCCCAGAAAATGTAGCTTTATTTTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCCCCCTTTTTGGTGACAAATTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGttt                                                                                                                                                                                                                <  6:1174858/149‑1 (MQ=255)
                                                                                                                                                                                        ttttGCACCCCAGCAAATGTAGGTTTATTGTTCCCTTCCTTGCTACAGAGTTCGACAGATACCCCGTTATGACTTTCTCCCTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTa                                                                                                                                                                                                               <  8:368316/148‑1 (MQ=255)
                                                                                                                                                                                        aTTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGttaa                                                                                                                                                                                                               >  6:1262213/1‑147 (MQ=255)
                                                                                                                                                                                         tttGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTac                                                                                                                                                                                                              >  6:233520/1‑149 (MQ=255)
                                                                                                                                                                                         atctccccccAGCAAATGTAGCTTTTTTTTTCCCTTCCTTGCTACAGAGTTCGACAGATACCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTac                                                                                                                                                                                                              <  6:496736/143‑1 (MQ=255)
                                                                                                                                                                                              aCCCCAGCAAATGTAGCGTTATTGTTACCTTCTTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgcacg                                                                                                                                                                                                         >  8:797566/1‑149 (MQ=255)
                                                                                                                                                                                              aCCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgcacg                                                                                                                                                                                                         >  5:1436854/1‑149 (MQ=255)
                                                                                                                                                                                                cccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGcc                                                                                                                                                                                                       >  6:1229329/1‑149 (MQ=255)
                                                                                                                                                                                                cccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGcc                                                                                                                                                                                                       >  8:1395996/1‑149 (MQ=255)
                                                                                                                                                                                                 ccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGTTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCt                                                                                                                                                                                                      >  8:182586/1‑149 (MQ=255)
                                                                                                                                                                                                 ccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCt                                                                                                                                                                                                      >  6:763050/1‑149 (MQ=255)
                                                                                                                                                                                                   aGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTgg                                                                                                                                                                                                    >  6:1357472/1‑149 (MQ=255)
                                                                                                                                                                                                   aGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTgg                                                                                                                                                                                                    >  5:320055/1‑149 (MQ=255)
                                                                                                                                                                                                    gCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGgc                                                                                                                                                                                                   >  6:298272/1‑149 (MQ=255)
                                                                                                                                                                                                          gTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGAtt                                                                                                                                                                                             >  5:128835/1‑149 (MQ=255)
                                                                                                                                                                                                            aGCGTTATTGTACCCTCCCTTGCTACAGAGTTCGACAGATATCCCGCTATAACATTCCCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTAt                                                                                                                                                                                           <  5:439845/149‑1 (MQ=255)
                                                                                                                                                                                                              cGTTATTTTTACCTTCCTTGCTACAGAGTTCGAAAGATACCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGc                                                                                                                                                                                         <  7:705608/149‑1 (MQ=255)
                                                                                                                                                                                                              cGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGc                                                                                                                                                                                         >  8:52964/1‑149 (MQ=255)
                                                                                                                                                                                                                 tATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcg                                                                                                                                                                                      >  5:1140157/1‑149 (MQ=255)
                                                                                                                                                                                                                  aTTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcgg                                                                                                                                                                                     >  7:394439/1‑149 (MQ=255)
                                                                                                                                                                                                                  aTTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGCTCCCGGAAATGCAGGACTACTTCCAGACGCTACTGACCGACATGCCGGAAAGTGGCAAAACGCATGATGCCCTTCGTACCTACGACGCTCCCCAGGGGAAAACGGa                                                       >  4:287784/1‑275 (MQ=255)
                                                                                                                                                                                                                      ttACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGTCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcggcggc                                                                                                                                                                                 >  5:757811/1‑149 (MQ=255)
                                                                                                                                                                                                                         ccttccttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGc                                                                                                                                                                                                                                                            <  5:1250930/71‑1 (MQ=255)
                                                                                                                                                                                                                             ccttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATc                                                                                                                                                                          <  6:645197/149‑1 (MQ=255)
                                                                                                                                                                                                                             ccttGCTACAGAGTTCGAAAGATACCCCGCTATGACATTCCCCCTTTTTGGCGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATc                                                                                                                                                                          <  7:336917/149‑1 (MQ=255)
                                                                                                                                                                                                                               ttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCg                                                                                                                                                                        >  6:812183/1‑149 (MQ=255)
                                                                                                                                                                                                                               ttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCg                                                                                                                                                                        <  7:449643/149‑1 (MQ=255)
                                                                                                                                                                                                                                   tACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGgcgc                                                                                                                                                                    >  7:1337665/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGgcgc                                                                                                                                                                    >  7:525380/1‑149 (MQ=255)
                                                                                                                                                                                                                                      agagTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCGTTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGa                                                                                                                                                                 >  8:101842/1‑149 (MQ=255)
                                                                                                                                                                                                                                       gagTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGAt                                                                                                                                                                >  7:1152449/1‑149 (MQ=255)
                                                                                                                                                                                                                                       gagTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGAt                                                                                                                                                                >  7:1121256/1‑149 (MQ=255)
                                                                                                                                                                                                                                       gagTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGAt                                                                                                                                                                >  6:969296/1‑149 (MQ=255)
                                                                                                                                                                                                                                        agTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACTGACCGACATGCTGGAAAGTGGCAAAGCGACTGATGCACTGTGTAACTACGACGGTCCACAGGGGAAAACGGAGCTACTCACACTGCTTGCCGGa                                 >  2:48236/1‑275 (MQ=255)
                                                                                                                                                                                                                                          ttCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATccc                                                                                                                                                             <  5:269977/149‑1 (MQ=255)
                                                                                                                                                                                                                                            cGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCgg                                                                                                                                                           <  6:830791/149‑1 (MQ=255)
                                                                                                                                                                                                                                            cGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCgg                                                                                                                                                           >  5:854353/1‑149 (MQ=255)
                                                                                                                                                                                                                                             gACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGTCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGa                                                                                                                                                          >  5:1208127/1‑149 (MQ=255)
                                                                                                                                                                                                                                             gACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGa                                                                                                                                                          >  6:331477/1‑149 (MQ=255)
                                                                                                                                                                                                                                             gACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGa                                                                                                                                                          >  8:72708/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aCAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGa                                                                                                                                                                                                                                  >  8:871639/1‑76 (MQ=255)
                                                                                                                                                                                                                                              aCAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGaa                                                                                                                                                         >  8:411053/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aCAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGaa                                                                                                                                                         >  8:292094/1‑149 (MQ=255)
                                                                                                                                                                                                                                               cAGATATCCCGCTATGCCATTCCCCTTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGaaa                                                                                                                                                        <  5:817791/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  atatCCCGCTATGACATTCTCCTTTTTTGGTGAAAAATTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGc                                                                                                                                                     <  7:1211335/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   tatCCCGCTATTACATTCTCCCTTTTTGGTGACAAATTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCa                                                                                                                                                    <  5:541618/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   tatCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCa                                                                                                                                                    >  7:625535/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   tatCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCa                                                                                                                                                    <  5:1400233/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    atCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAg                                                                                                                                                   <  5:370368/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           tATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTac                                                                                                                                                                                                              >  7:1391063/1‑83 (MQ=255)
                                                                                                                                                                                                                                                            aTGACATGCCCCATTTTTGGTGAAAAATTTCCCCCCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGCTCCCGGAAATGCAGGACTACtt                                                                                                                                           <  7:873907/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGACATTCTCCCTTTTTGGTGACAAATTTCCCCGCCACTCCGGCACTACGCTGTCGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTc                                                                                                                                          <  7:1257748/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTc                                                                                                                                          >  5:478249/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              gACTTTCTCCTTTTTTGGTGACAATTTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTcc                                                                                                                                         <  7:763922/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              gACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTcc                                                                                                                                         >  5:1469728/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 aTTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGa                                                                                                                                      >  7:213862/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    ctcCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGc                                                                                                                                   <  8:494767/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tcCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCt                                                                                                                                  >  8:1116780/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     tcCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATGATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCt                                                                                                                                  >  6:1053589/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       ccTTTTTGGTGACAATTTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTAc                                                                                                                                <  7:292094/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ccTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCCCGCCTGGCGCGATTATGCTCGGCGGCGGTAATCAGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACTGACCGACATCCTGGAAAGTGGCAAAGCGACTGATGCACTGTGTAACTTCGACGGTCCACAGGGGAAAACGGAGCTACTCGCACTGTGTGCCGGAATCCCGGGCGCGAAGTTGGGTTGGGacac    >  4:191696/1‑270 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                 
CTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAACTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACTGACCGACATGCTGGAAAGTGGCAAAGCGACTGATGCACTGTGTAACTACGACGGTCCACAGGGGAAAACGGAGCTACTCACACTGCTTGCCGGAATGCTGCGCGAGAAGTTGGGTTGGGATATCG  >  NC_000913/3739453‑3739990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: