Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,790,303 A→T 5.8% intergenic (‑222/+17) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,3030AT 5.8% 2560.7 / 99.6 709intergenic (‑222/+17)waaH/tdhLPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (350/316);  new base T (20/21);  total (371/338)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

Only 100 of 711 total aligned reads displayed.

ATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCA  >  NC_000913/3790037‑3790564
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                      
                                                                     aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTAGTGTTAATCCCAGCTCAGAATAACTTTCACGGCCTGGCCCGAACGAATAGCGTcctgtatctt                                                                                                                                                                                            >  1:74092/1‑265 (MQ=255)
                                                                                                                       tattatTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAc                                                                                                                                                                                                                                                                       <  6:1391320/149‑1 (MQ=255)
                                                                                                                        attattTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTcc                                                                                                                                                                                                                                                                      <  8:390097/149‑1 (MQ=255)
                                                                                                                         ttattTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCa                                                                                                                                                                                                                                                                     <  8:333748/149‑1 (MQ=255)
                                                                                                                          tattTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                                                                                                                                                                                                    >  5:712165/1‑149 (MQ=255)
                                                                                                                            tttttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGcc                                                                                                                                                                                                                                                                  <  7:1083344/149‑1 (MQ=255)
                                                                                                                               ttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCtt                                                                                                                                                                                                                                                               >  7:1133644/1‑149 (MQ=255)
                                                                                                                                tACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTg                                                                                                                                                                                                                                                              <  5:493509/149‑1 (MQ=255)
                                                                                                                                   aGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTc                                                                                                                                                                                                                                                           >  5:304862/1‑149 (MQ=255)
                                                                                                                                     gTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgt                                                                                                                                                                                                                                                         <  5:1016570/149‑1 (MQ=255)
                                                                                                                                     gTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgt                                                                                                                                                                                                                                                         >  5:350082/1‑149 (MQ=255)
                                                                                                                                      tGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                                                                                                                                                                                        >  7:233545/1‑149 (MQ=255)
                                                                                                                                      tGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                                                                                                                                                                                        >  5:261839/1‑149 (MQ=255)
                                                                                                                                       gagagaGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAATAGGGCTGGAATACCAGCCCTTGTTCgttt                                                                                                                                                                                                                                                       <  7:931753/149‑3 (MQ=255)
                                                                                                                                       gagagaGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                                                                                                                                                                                       <  7:830847/149‑1 (MQ=255)
                                                                                                                                             gTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATcc                                                                                                                                                                                                                                                 <  8:213067/149‑1 (MQ=255)
                                                                                                                                              tCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATccc                                                                                                                                                                                                                                                <  8:1012367/149‑1 (MQ=255)
                                                                                                                                               cACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCTAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCa                                                                                                                  >  2:153170/1‑274 (MQ=255)
                                                                                                                                                aCTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAg                                                                                                                                                                                                                                              >  7:363036/1‑149 (MQ=255)
                                                                                                                                                          agagagATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                                                                                                                                                                                                                                    <  6:511239/149‑1 (MQ=255)
                                                                                                                                                          agagagATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                                                                                                                                                                                                                                    <  7:1434964/149‑1 (MQ=255)
                                                                                                                                                           gagagaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                                                                                                                                                                                                   <  6:466070/149‑1 (MQ=255)
                                                                                                                                                            agagaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACt                                                                                                                                                                                                                                  <  6:965404/149‑1 (MQ=255)
                                                                                                                                                             gagaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACtt                                                                                                                                                                                                                                 >  6:839111/1‑149 (MQ=255)
                                                                                                                                                             gagaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACtt                                                                                                                                                                                                                                 <  6:1107216/149‑1 (MQ=255)
                                                                                                                                                              agaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                                                                                                                                                                                                                                >  8:1284971/1‑149 (MQ=255)
                                                                                                                                                               gaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                                                                                                                                                                                                                                    >  5:592561/1‑144 (MQ=255)
                                                                                                                                                                aTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTcc                                                                                                                                                                                                                              >  6:359857/1‑149 (MQ=255)
                                                                                                                                                                 tAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTccc                                                                                                                                                                                                                             >  5:998794/1‑149 (MQ=255)
                                                                                                                                                                   aTGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCgg                                                                                                                                                                                                                           >  7:645365/1‑149 (MQ=255)
                                                                                                                                                                    tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGa                                                                                                                                                                                                                          >  5:1142894/1‑149 (MQ=255)
                                                                                                                                                                    tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGa                                                                                                                                                                                                                          <  6:614169/149‑1 (MQ=255)
                                                                                                                                                                     gagaGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGAc                                                                                                                                                                                                                         >  7:1390122/1‑149 (MQ=255)
                                                                                                                                                                          cagcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                                                                                                                                                                                                    <  6:299446/149‑1 (MQ=255)
                                                                                                                                                                              tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa                                                                                                                                                                                                                <  8:1221656/149‑1 (MQ=255)
                                                                                                                                                                                  taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCa                                                                                                                                                                                                            >  5:538295/1‑149 (MQ=255)
                                                                                                                                                                                  taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCa                                                                                                                                                                                                            <  8:596228/149‑1 (MQ=255)
                                                                                                                                                                                   aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCAt                                                                                                                                                                                                           >  5:807977/1‑149 (MQ=255)
                                                                                                                                                                                      agaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                                                                                                                                                                                        <  6:783114/149‑1 (MQ=255)
                                                                                                                                                                                      agaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                                                                                                                                                                                        <  8:1124731/149‑1 (MQ=255)
                                                                                                                                                                                      agaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                                                                                                                                                                                        <  6:1224818/149‑1 (MQ=255)
                                                                                                                                                                                        aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAgc                                                                                                                         >  1:33440/1‑226 (MQ=255)
                                                                                                                                                                                         tCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTc                                                                                                                                                                                                     <  7:448975/149‑1 (MQ=255)
                                                                                                                                                                                                 aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCt                                                                                                                                                                                             <  6:1142894/149‑1 (MQ=255)
                                                                                                                                                                                                  ttACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCtt                                                                                                                                                                                            >  7:310321/1‑149 (MQ=255)
                                                                                                                                                                                                      gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                                                                                                                                                                                        >  8:770578/1‑149 (MQ=255)
                                                                                                                                                                                                      gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                                                                                                                                                                                        >  6:227286/1‑149 (MQ=255)
                                                                                                                                                                                                      gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                                                                                                                                                                                        >  6:1043233/1‑149 (MQ=255)
                                                                                                                                                                                                        aCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACt                                                                                                                                                                                                                        >  8:1146843/1‑115 (MQ=255)
                                                                                                                                                                                                        aCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGc                                                                                                                                                                                                                     >  8:1099054/1‑118 (MQ=255)
                                                                                                                                                                                                          tctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAat                                                                                                                                                                                    >  5:847468/1‑149 (MQ=255)
                                                                                                                                                                                                            tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatca                                                                                                                                                                                  >  6:814923/1‑149 (MQ=255)
                                                                                                                                                                                                                 taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt                                                                                                                                                                             >  7:694/1‑149 (MQ=255)
                                                                                                                                                                                                                  acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATa                                                                                                                                                                            <  7:1117754/149‑1 (MQ=255)
                                                                                                                                                                                                                   cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCtt                                                                                                                                                                                                                                                               <  5:651835/65‑1 (MQ=255)
                                                                                                                                                                                                                    acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATaga                                                                                                                                                                          >  5:899954/1‑149 (MQ=255)
                                                                                                                                                                                                                      tGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACGAGCCCTTGTTCGTGCTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCGCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGGGAAACGATGGGTAATGAACGGCGAAAGAACGAGGCCAGACTGAATAAGACCCGCCATCATGTACCCAGTTT‑ACAAAACTAACCGCCCGTAAAGAACCTTAATGAACAAGCATTTAACAAAAACTTTgg                                         >  4:224334/1‑275 (MQ=255)
                                                                                                                                                                                                                      tGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCACACATATCA‑CGACCGTAAATACCTTTAATGAACAAGCATTTAAAAATCACTTTgt                                         >  1:11064/1‑274 (MQ=255)
                                                                                                                                                                                                                        aCAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCGTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGaaa                                                                                                                                                                      >  7:1312721/1‑149 (MQ=255)
                                                                                                                                                                                                                        aCAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGaaa                                                                                                                                                                      >  6:586628/1‑149 (MQ=255)
                                                                                                                                                                                                                          aGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAg                                                                                                                                                                                                 <  2:348608/120‑1 (MQ=255)
                                                                                                                                                                                                                            gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAt                                                                                                                                                                  <  8:693631/149‑1 (MQ=255)
                                                                                                                                                                                                                            gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTc                                       <  4:164532/271‑1 (MQ=255)
                                                                                                                                                                                                                              ttttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATgg                                                                                                                                                                >  6:143553/1‑149 (MQ=255)
                                                                                                                                                                                                                              ttttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATACAGAAACGATgg                                                                                                                                                                <  7:1281668/149‑1 (MQ=255)
                                                                                                                                                                                                                                ttACATATTTAACATATTACCCNNCGATANAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGAt                               <  3:12905/275‑1 (MQ=255)
                                                                                                                                                                                                                                  aCATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCg                                                                                                                                                                                                                  <  4:267731/95‑1 (MQ=255)
                                                                                                                                                                                                                                     tatTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGa                                                                                                                                                         >  5:140667/1‑149 (MQ=255)
                                                                                                                                                                                                                                      atTTAACGGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGAt                                                                                                                                                        <  2:27730/149‑1 (MQ=255)
                                                                                                                                                                                                                                       tttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                                                                       >  6:706328/1‑149 (MQ=255)
                                                                                                                                                                                                                                         tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCt                                                                                                                                                                                          >  6:936258/1‑112 (MQ=255)
                                                                                                                                                                                                                                           aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCg                                                                                                                                                   >  7:150068/1‑149 (MQ=255)
                                                                                                                                                                                                                                            cAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGa                                                                                                                                                  <  8:55302/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   tCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCg                                                                                                                                           >  8:283226/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ccTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGa                                                                                                                                          <  7:554880/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAg                                                                                                                                         <  7:1204126/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATAc                                                                          <  2:64467/211‑1 (MQ=255)
                                                                                                                                                                                                                                                      tCAGATAAAAGGGCTGGAATACTAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTGTCCCGGACTGGTCCGAACGCATAGCGTCAAAGCCCTTCTGTCAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATGGAgg                                                                                                                                        >  8:908953/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa                                                                                                                                                                                     <  8:1342125/104‑1 (MQ=255)
                                                                                                                                                                                                                                                      tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGaaa                                                                                                                                                >  6:894509/1‑141 (MQ=255)
                                                                                                                                                                                                                                                        aGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGcc                                                                                                                                      >  8:865413/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCa                                                                                                                                     >  5:1334926/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCa                                                                                                                                     <  8:1421204/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGAACGGCGACAGATCGAGGCCAGACTGAATCAgcg                                                                                                                        >  2:264802/1‑162 (MQ=255)
                                                                                                                                                                                                                                                          aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAg                                                                                                                                    >  5:1265932/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           tAAAAGGTCTGGATTACCAGCCCTTGTTCGTCTTAATCCCCGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAGGCCCTTCTGGAAATCACCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAg    <  4:327922/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGAc                                                                                                                                  >  5:9275/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             aaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCa                                     <  4:44010/240‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGt                                                                                                                                                                                                      <  6:1314377/79‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGAt                                                                                                                                             >  8:447781/1‑136 (MQ=255)
                                                                                                                                                                                                                                                              aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTg                                                                                                                                >  8:1393275/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              aaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTg                                                                                                                                >  7:396379/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                gggCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGaa                                                                                                                              >  8:720658/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 ggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAt                                                                                                                             >  5:172331/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCa                                                                                                                           <  5:417593/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAACGATCGGCGAAAGATCGAGGCCAGACTGAATCa                                                                                                                           >  8:150320/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    tGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAg                                                                                                                                         >  7:724252/1‑134 (MQ=255)
                                                                                                                                                                                                                                                                    tGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAg                                                                                                                          >  5:1063398/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      gAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGa                                                                                                                                                                              <  2:191016/95‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGcgccg                                                                                                                     <  3:63630/150‑1 (MQ=255)
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                      
ATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCA‑CGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCA  >  NC_000913/3790037‑3790564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑