Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,878,894 T→A 6.4% D409V (GAC→GTC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,878,8940TA 6.4% 494.4 / 18.0 140D409V (GAC→GTC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (72/59);  new base A (5/4);  total (77/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 143 total aligned reads displayed.

AACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACG  >  NC_000913/3878652‑3879154
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                     
  cggcttgtGTCCGGGTTGTCTTCGGCACGCCCGATACTACAGCCAAGCGCGGTGTGAAGCGTCGCCACGTCCTGAGAAGAGGGCAGCTTAGCGAAGCGCGCTTGTGCGACTTTGAGGATTTTCCCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGTCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAg                                                                                                                                                                                                                                     <  4:95233/268‑1 (MQ=255)
    aaGTTTGTCCGGGTTGTACTCGTAACGAACGGTACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAgcg                                                                                                                                                                                                                                   <  2:344620/274‑1 (MQ=255)
     atgttGTCCGGCTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTTAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCGTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAgcg                                                                                                                                                                                                                                   <  2:194362/271‑1 (MQ=255)
                                           gCCAAGCGCGGTGATAAGCGTCGCGACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGcgccg                                                                                                                                                                                            <  2:14296/275‑1 (MQ=255)
                                                gggcgGGGGTAGGCGTCGCCACTTCCGGNGNTGAGGGCCTCTTTGCGAAGGGCGCTTTCTCGACGGTGAGGGTTTTNCCCTTCTGCGGCAGAGTCGCCTGGTTCTGGCGGTTACGCCCCTGCTTCGCAGGGCCGCCCGCGGAGTCCNCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTca                                                                                                                                                                                       <  3:33056/273‑1 (MQ=255)
                                                gcgcGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTca                                                                                                                                                                                       <  2:85967/275‑1 (MQ=255)
                                                                cGCCCCTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGCAAAGCGCCGCATCACGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGGTACGTCGGGCGCCCCTTTACGGCGGGTCGTTTCACACCCGCCACGCTCCGTCTcccggggccggggcgcc                                                                                                                                                                        >  4:252981/1‑269 (MQ=255)
                                                                                   agCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCCTGAACCCCCGCGTCACGGGCCCGGGCGGCATCGGATATTTTGTCAAcccc                                                                                                                                                    >  2:351587/1‑273 (MQ=255)
                                                                                              gAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGt                                                                                                                                                                                                                                                                       >  6:668767/1‑149 (MQ=255)
                                                                                              gAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGt                                                                                                                                                                                                                                                                       <  7:1434613/149‑1 (MQ=255)
                                                                                               aaGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTc                                                                                                                                                                                                                                                                      <  5:788173/149‑1 (MQ=255)
                                                                                                aGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCt                                                                                                                                                                                                                                                                     <  6:497095/149‑1 (MQ=255)
                                                                                                aGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCt                                                                                                                                                                                                                                                                     <  5:668767/149‑1 (MQ=255)
                                                                                                aGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCt                                                                                                                                                                                                                                                                     >  7:469965/1‑149 (MQ=255)
                                                                                                      cTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGACTGCCAgg                                                                                                                                                                                                                                                               <  6:1005403/149‑2 (MQ=255)
                                                                                                       tttCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGtt                                                                                                                                                                                                                                                              >  5:242645/1‑149 (MQ=255)
                                                                                                       tttCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGACTGCCAgga                                                                                                                                                                                                                                                              <  6:1217658/149‑3 (MQ=255)
                                                                                                        ttCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGttt                                                                                                                                                                                                                                                             <  8:305857/149‑1 (MQ=255)
                                                                                                        ttCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGACTGCCAggaa                                                                                                                                                                                                                                                             <  8:1118957/149‑4 (MQ=255)
                                                                                                           tcGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGcc                                                                                                                                                                                                                                                          >  8:480659/1‑149 (MQ=255)
                                                                                                           tcGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGcc                                                                                                                                                                                                                                                          >  7:227523/1‑149 (MQ=255)
                                                                                                             gACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCg                                                                                                                                                                                                                                                        >  5:1195927/1‑149 (MQ=255)
                                                                                                                        ttttACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCt                                                                                                                                                                                                                                             <  8:227523/149‑1 (MQ=255)
                                                                                                                           tACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGttt                                                                                                                                                                                                                                                             <  5:828041/130‑1 (MQ=255)
                                                                                                                           tACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAg                                                                                                                                                                                                                                          >  7:687166/1‑149 (MQ=255)
                                                                                                                            aCCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGt                                                                                                                                                                                                                                         <  6:733609/149‑1 (MQ=255)
                                                                                                                             cccTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGc                                                                                                                                                                                                                                                           <  5:1475862/130‑1 (MQ=255)
                                                                                                                             cccTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGc                                                                                                                                                                                                                                                           >  6:1475862/1‑130 (MQ=255)
                                                                                                                              ccTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGc                                                                                                                                                                                                                                                      >  4:62151/1‑134 (MQ=255)
                                                                                                                              ccTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCg                                                                                                                                                                                                                                       >  6:708299/1‑149 (MQ=255)
                                                                                                                              ccTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCg                                                                                                                                                                                                                                       >  8:1356078/1‑149 (MQ=255)
                                                                                                                                           aaTCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAc                                                                                                                                                                                                                          <  6:455237/149‑1 (MQ=255)
                                                                                                                                           aaTCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAc                                                                                                                                                                                                                          >  8:473930/1‑149 (MQ=255)
                                                                                                                                               gCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAcggcg                                                                                                                                                                                                                      >  6:129180/1‑149 (MQ=255)
                                                                                                                                                ccTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAcggcgg                                                                                                                                                                                                                     <  8:1193885/149‑1 (MQ=255)
                                                                                                                                                 cTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCggg                                                                                                                                                                                                                    >  5:1084250/1‑149 (MQ=255)
                                                                                                                                                 cTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCggg                                                                                                                                                                                                                    >  5:109532/1‑149 (MQ=255)
                                                                                                                                                 cTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCggg                                                                                                                                                                                                                    >  7:786752/1‑149 (MQ=255)
                                                                                                                                                  tGGTTATTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTGCAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGt                                                                                                                                                                                                                   >  8:652795/1‑149 (MQ=255)
                                                                                                                                                       cTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCAttt                                                                                                                                                                                                              <  5:1106899/149‑1 (MQ=255)
                                                                                                                                                              ttACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCAcgcgc                                                                                                                                                                                                       >  6:965735/1‑149 (MQ=255)
                                                                                                                                                                  gCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCAtt                                                                                                                                                                                                               >  7:682328/1‑137 (MQ=255)
                                                                                                                                                                  gCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCAtt                                                                                                                                                                                                               <  8:682328/137‑1 (MQ=255)
                                                                                                                                                                    cccTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGt                                                                                                                                                                                                                                                               <  5:980689/87‑1 (MQ=255)
                                                                                                                                                                    cccTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGt                                                                                                                                                                                                                                                               >  6:980689/1‑87 (MQ=255)
                                                                                                                                                                    cccTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGAcg                                                                                                                                                                                                 >  7:724652/1‑149 (MQ=255)
                                                                                                                                                                    cccTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGAcg                                                                                                                                                                                                 >  8:336470/1‑149 (MQ=255)
                                                                                                                                                                    cccTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGACTGCCAggaa                                                                                                                                                                                                                                                             >  5:1321989/1‑86 (MQ=255)
                                                                                                                                                                     gcTGCTTCGCAGAGCCGCCCGCGCAGTCCCCGCCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCa                                                                 <  2:126207/274‑1 (MQ=255)
                                                                                                                                                                     ccTGCTTCGCAGAGCCGCCCGCGGAGTCGCCATCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCGGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCCCGCGCGCGAcgc                                                                                                                                                                                                >  8:893723/1‑149 (MQ=255)
                                                                                                                                                                      cTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGAcgcg                                                                                                                                                                                               >  7:1046499/1‑149 (MQ=255)
                                                                                                                                                                      cTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGAcgcg                                                                                                                                                                                               >  8:439984/1‑149 (MQ=255)
                                                                                                                                                                       tGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACgcgc                                                                                                                                                                                              >  8:1001080/1‑149 (MQ=255)
                                                                                                                                                                             gCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTc                                                                                                                                                                                        >  6:553770/1‑149 (MQ=255)
                                                                                                                                                                             gCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTc                                                                                                                                                                                        >  6:1439699/1‑149 (MQ=255)
                                                                                                                                                                                 agCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTCTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgg                                                                                                                                                                                    <  8:469965/149‑1 (MQ=255)
                                                                                                                                                                                        cgcgGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggcacggg                                                                                                                                                                             <  5:1439699/149‑1 (MQ=255)
                                                                                                                                                                                         gcgGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCa                                                                                                                                                                                                                                 >  6:443589/1‑96 (MQ=255)
                                                                                                                                                                                         gcgGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCa                                                                                                                                                                                                                                 <  5:443589/96‑1 (MQ=255)
                                                                                                                                                                                             ataagggacaggccaGGAACAGTTCGGAAAGCGCCGGATCGCGTTCCGGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCTGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGAACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGt                                          <  2:253874/262‑1 (MQ=255)
                                                                                                                                                                                               tCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATc                                                                                                                                                                      >  6:396201/1‑149 (MQ=255)
                                                                                                                                                                                                 cccTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCAc                                                                                                                                                                                                           <  8:343329/110‑1 (MQ=255)
                                                                                                                                                                                                 cccTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCAc                                                                                                                                                                                                           >  7:343329/1‑110 (MQ=255)
                                                                                                                                                                                                 cccTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCAc                                                                                                                                                                                                           <  7:150127/110‑1 (MQ=255)
                                                                                                                                                                                                  ccTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCCCGGGCACGGGCAGCATCGAt                                                                                                                                                                   >  7:873411/1‑149 (MQ=255)
                                                                                                                                                                                                  ccTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAt                                                                                                                                                                   >  8:1335032/1‑149 (MQ=255)
                                                                                                                                                                                                   cTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATa                                                                                                                                                                  >  5:1005324/1‑149 (MQ=255)
                                                                                                                                                                                                   cTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATa                                                                                                                                                                  >  5:862461/1‑149 (MQ=255)
                                                                                                                                                                                                        accaGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAAtttt                                                                                                                                                             <  5:93094/149‑1 (MQ=255)
                                                                                                                                                                                                        accaGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAAtttt                                                                                                                                                             <  8:786752/149‑1 (MQ=255)
                                                                                                                                                                                                         ccaGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTg                                                                                                                                                            >  5:38647/1‑149 (MQ=255)
                                                                                                                                                                                                           aGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGcc                                                                                                                                                          >  5:463460/1‑149 (MQ=255)
                                                                                                                                                                                                              tACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAAc                                                                                                                                                       <  5:825954/149‑1 (MQ=255)
                                                                                                                                                                                                                cAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCa                                                                                                                                                     <  6:848640/149‑1 (MQ=255)
                                                                                                                                                                                                                  gTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACg                                                                                                                                                   >  8:539249/1‑149 (MQ=255)
                                                                                                                                                                                                                  gTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCACTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACg                                                                                                                                                   >  6:862618/1‑149 (MQ=255)
                                                                                                                                                                                                                    tCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGAt                                                                                                                                                 <  8:1197111/149‑1 (MQ=255)
                                                                                                                                                                                                                     cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGAtt                                                                                                                                                >  8:1139283/1‑149 (MQ=255)
                                                                                                                                                                                                                     cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGAtt                                                                                                                                                <  5:129180/149‑1 (MQ=255)
                                                                                                                                                                                                                      ggAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGAttt                                                                                                                                               >  8:820573/1‑149 (MQ=255)
                                                                                                                                                                                                                        aaaGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTc                                                                                                                                             <  7:1191977/149‑1 (MQ=255)
                                                                                                                                                                                                                         aaGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCCACCACGATTTTcg                                                                                                                                            >  5:1409976/1‑149 (MQ=255)
                                                                                                                                                                                                                          aGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTcgc                                                                                                                                           <  7:1356078/149‑1 (MQ=255)
                                                                                                                                                                                                                          aGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTcgc                                                                                                                                           >  6:325019/1‑149 (MQ=255)
                                                                                                                                                                                                                            cgcCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGt                                                                                                                                         <  7:1220735/149‑1 (MQ=255)
                                                                                                                                                                                                                             gcCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGGTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAAACAGTTTGGCTTTGGTTTGGGAGGACAATTTCGGGTCCGGCACTTTCAc         >  2:244069/1‑275 (MQ=255)
                                                                                                                                                                                                                               cGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCgg                                                                                                                                      <  5:240936/149‑1 (MQ=255)
                                                                                                                                                                                                                                  aTCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTg                                                                                                                                   >  7:619613/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCCCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTgg                                                                                                                                  >  8:1035114/1‑149 (MQ=255)
                                                                                                                                                                                                                                   tCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTgg                                                                                                                                  >  8:921350/1‑149 (MQ=255)
                                                                                                                                                                                                                                      cgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCCCGACGCGCCGCTTTCCGGGCACGGGCAGCCTCCATAATTTTGTCAACCACGCTTTTCGCGCCGGTTTGGGTTTCCAGAAGTGAATCTGCCTGCCTATCGTTCCTCTGCCTTTTCAACCCCCATTTCCCCCTCGAAGAATCCCGTTTGTCCTTGTTCTGGGGCGAAGATTTCGTGTCCCGTCCTTTctctgcaaac  >  4:175606/1‑264 (MQ=255)
                                                                                                                                                                                                                                        aaacTGGCAGACTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGtttt                                                                                                                             >  8:213029/4‑149 (MQ=255)
                                                                                                                                                                                                                                        aaacTGGCAGACTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGtttt                                                                                                                             >  5:26945/4‑149 (MQ=255)
                                                                                                                                                                                                                                         tCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTc                                                                                                                            <  8:1116721/149‑1 (MQ=255)
                                                                                                                                                                                                                                         aacTGGCAGACTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTc                                                                                                                            >  5:1267479/3‑149 (MQ=255)
                                                                                                                                                                                                                                           cTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                                                                                                                          >  6:1426583/1‑149 (MQ=255)
                                                                                                                                                                                                                                           cTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                                                                                                                          <  6:972656/149‑1 (MQ=255)
                                                                                                                                                                                                                                            tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTTGCGGCGGTTGGGTTTTCcag                                                                                                                         >  5:873074/1‑149 (MQ=255)
                                                                                                                                                                                                                                              gCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                                                                                                                       <  5:553770/149‑1 (MQ=255)
                                                                                                                                                                                                                                              gCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                                                                                                                       <  7:1268715/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                     
AACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACG  >  NC_000913/3878652‑3879154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: