| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 533,846 | T→C | 5.7% | intergenic (+20/‑70) | allR → / → gcl | glyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 533,846 | 0 | T | C | 5.7% | 813.5 / 26.0 | 246 | intergenic (+20/‑70) | allR/gcl | glyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase |
| Reads supporting (aligned to +/- strand): ref base T (121/110); new base C (7/7); total (128/118) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-03 | |||||||||||
Only 100 of 253 total aligned reads displayed.
TGAAGGACCTGGAACAAGCGCGTGAACTGGGCTATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGG > NC_000913/533588‑534115 | tGAAGGACCTGGAACAAGCGCGTGAACTGGGCTATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATAACAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTAGCACCCCGCTCTGGGGAGCGGG‑tttttt > 4:303425/1‑275 (MQ=255) ggCTATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATATCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCACGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTTACAGTTGGAAAAAtttt > 2:105192/1‑275 (MQ=255) tggATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGGGTTGTTGTCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaataaata < 1:5519/273‑1 (MQ=255) aaaGAGGAGGATGTTGTAGGTCTGAATTGAAAAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGAACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAgg < 2:187927/274‑1 (MQ=255) aaTTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGCACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAACAATTTTCCAATAAATAGAGGCAGAGAAAAAATGGCaaaacggagg > 4:272279/1‑269 (MQ=255) cATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAAttt > 1:9168/1‑230 (MQ=255) gCTTAAACAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCCTCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCa < 3:9168/230‑1 (MQ=255) tttCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAc < 2:303425/273‑1 (MQ=255) ttgttgCCACTATCACCATCGCCGGGCCTTCATCAAGACTGAAAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGAGATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGATCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTGCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGt < 2:239035/274‑1 (MQ=255) gttgCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGctct > 8:404508/1‑149 (MQ=255) cGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGa > 5:675393/1‑149 (MQ=255) gCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttttt > 4:162716/1‑158 (MQ=255) gCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGATGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAg > 7:911750/1‑149 (MQ=255) atctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCgg < 6:243091/149‑1 (MQ=255) tctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGa < 2:162716/158‑1 (MQ=255) ctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggga < 7:101738/149‑2 (MQ=255) tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCAACCCGCTCCGCAGAGCgggat < 6:1239618/149‑3 (MQ=255) ccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttt < 6:137615/149‑1 (MQ=255) catctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggga > 7:1377439/1‑145 (MQ=255) ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttttttt < 8:619984/149‑1 (MQ=255) ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttttttt < 6:1093981/149‑1 (MQ=255) ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttttttt < 5:997742/149‑1 (MQ=255) ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑ttttttt < 8:1166989/149‑1 (MQ=255) ccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGa < 5:175624/149‑1 (MQ=255) ttCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAAtt > 7:462863/1‑149 (MQ=255) catcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCACTAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTTACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGc > 2:4052/1‑273 (MQ=255) atcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGa > 7:655063/1‑149 (MQ=255) tcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGaa > 6:1442971/1‑149 (MQ=255) tcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGaa > 6:1163896/1‑149 (MQ=255) aCTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTg < 6:55419/149‑1 (MQ=255) gaagaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGAGTGAAAGTACATCCATAATGTCTGTAGCATCCCGCTCTTCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAAt > 5:1059368/1‑149 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc < 8:1020515/149‑1 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc > 8:458519/1‑149 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc < 8:813667/149‑1 (MQ=255) tCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTcc < 5:265909/149‑1 (MQ=255) gTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaa > 5:572620/1‑149 (MQ=255) gTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaa < 8:744778/149‑1 (MQ=255) ttttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAATTTTTCCaac < 6:829707/149‑2 (MQ=255) cagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGt < 5:532752/149‑1 (MQ=255) agGGTGAGCTGGTCGGAGACACCGCCCGCGATATCAGCACGGCGTGGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAAGAGAGGTa > 8:1364315/1‑149 (MQ=255) gggTGGGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCa < 2:314621/275‑1 (MQ=255) cTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaaa > 5:1088209/1‑149 (MQ=255) gTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGa < 6:392556/96‑1 (MQ=255) tCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaa > 7:162445/1‑144 (MQ=255) agagACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCa > 8:1437991/1‑149 (MQ=255) agaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaa > 5:1235756/1‑149 (MQ=255) agaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaa > 5:809132/1‑149 (MQ=255) agaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaa > 6:1336339/1‑149 (MQ=255) gaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaaa > 8:219012/1‑149 (MQ=255) cacCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAAt < 7:1137082/149‑1 (MQ=255) acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg > 5:856907/1‑149 (MQ=255) acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg < 7:219012/149‑1 (MQ=255) acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg > 6:485060/1‑149 (MQ=255) acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg > 6:284661/1‑149 (MQ=255) ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATga < 5:1357903/149‑1 (MQ=255) cgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgag < 7:812268/149‑1 (MQ=255) gcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgaga < 7:1043352/149‑1 (MQ=255) cgcgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGcc < 5:539970/149‑1 (MQ=255) gcgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCg > 7:838799/1‑149 (MQ=255) tCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAg < 5:326424/117‑1 (MQ=255) tCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcg > 6:1073028/1‑149 (MQ=255) cAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcgc < 5:1332280/149‑1 (MQ=255) aGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcgcg > 5:932336/1‑149 (MQ=255) ggCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCGCTGCGGAGCGGGTTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAg < 2:97811/275‑1 (MQ=255) cGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgt < 7:386265/149‑1 (MQ=255) cGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgt < 7:1437991/149‑1 (MQ=255) cGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGACGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCCCTACCGCCTTCGCTCTTCCGGGAGCTGCACTCAATCCGTTCTCCCCAGCGATGCGTAGGCACGGCGGTATTCGCCACCTTCTGGCCCGTCATGTGGCACGTg > 4:10817/1‑275 (MQ=255) ggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTAtgtg < 6:813490/149‑1 (MQ=255) aCTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCGATGTATGTGCt > 8:150033/1‑149 (MQ=255) tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTgg < 8:1133743/149‑1 (MQ=255) tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTgg > 6:1312144/1‑149 (MQ=255) gAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGtt > 2:357119/1‑180 (MQ=255) aaaGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGcc < 6:59/122‑1 (MQ=255) aaaGCACATCCATAATGTCTGTCGCAACCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTGTACAGTTGTAAAAATTTTCCAATAAATAGAGGTAGGAAAAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGgag > 8:1060329/1‑149 (MQ=255) gCACATCCATAATGTCTTTCGCNTCCCNCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg < 4:357119/180‑1 (MQ=255) aaaaTCAATGATGTCGTTCGCGTCCCGCTNNTCGTGGAGAG‑TGTTTATGTAAAAATGTGAANGGTGGAAAAATTTTGCAAAAAAAGTAGGTAGGAATAAAATGGCAAAAAAGAGAGCCGGTGACGCGGCAATGTATNGGCTGGAGAAAGAAGTGCTCACGACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTGTAATCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCgg < 3:51542/272‑1 (MQ=255) caTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa < 5:602504/149‑1 (MQ=255) aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAaagaag < 7:473477/149‑1 (MQ=255) tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAAtttt > 8:260060/1‑72 (MQ=255) tCCATAATGCCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAAtttt < 7:260060/72‑1 (MQ=255) cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTa > 7:974703/1‑149 (MQ=255) cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGtt < 4:64884/192‑1 (MQ=255) aTAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTAt < 7:881774/149‑1 (MQ=255) tAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATc < 6:910315/149‑1 (MQ=255) aaTGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGaa < 5:814195/87‑1 (MQ=255) tctgtcGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGACAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCAAATGGCGGAAGGtttacaccg > 4:225005/1‑268 (MQ=255) tgtcGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCg > 6:232367/1‑149 (MQ=255) tgtcGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCg > 6:793974/1‑149 (MQ=255) tgtcGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCTTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCcgcg > 2:205814/1‑275 (MQ=255) tcGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCgcgcc > 1:50612/1‑274 (MQ=255) aaaaacccGCTCCGCAGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa > 8:886446/6‑133 (MQ=255) aTCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg > 8:291050/1‑149 (MQ=255) aTCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg < 8:1312832/149‑1 (MQ=255) tCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCgg > 6:639927/1‑149 (MQ=255) acccGCTCCGCAGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag < 5:235380/66‑1 (MQ=39) cccGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgt < 8:280092/149‑1 (MQ=255) ccGCTCCGCAGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgtg > 7:719281/1‑149 (MQ=255) cgtgctGGGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAAGAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAgg < 4:205814/271‑1 (MQ=255) ctctGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTc > 8:532299/1‑149 (MQ=255) tGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg > 5:126611/1‑149 (MQ=255) | TGAAGGACCTGGAACAAGCGCGTGAACTGGGCTATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGG‑TTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGG > NC_000913/533588‑534115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |