Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,413 T→G 5.1% intergenic (‑12/+102) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4130TG 5.1% 631.5 / 13.3 185intergenic (‑12/+102)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (82/91);  new base G (3/7);  total (86/99)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

ACTGATATTCCTGCCCCTGATAGCGGTAACAGCCGTTGGCGATCCGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCGCCAGCGGCATCTCTTCGGGTTTGTGCGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAT  >  NC_000913/4070140‑4070600
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                            
aCTGATATTCCTGCCCCTGATAGCGGTAACAGCCGTTGGCGATCCGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCGCCAGCGGCATCTCTTCGGGTTTGTACGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCACCCGCGGCAAGTGAAAAGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGAAACTTAATTACAGACATACAGCCTCACGt                                                                                                                                                                                              >  1:30626/1‑274 (MQ=255)
            gCCCCTGATAGCGGTAACAGCCGTTGGCGATCCGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCGCCAGCGGCATCTCTTCGGGTTTGTGCGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCa                                                                                                                                                                                >  2:219517/1‑275 (MQ=255)
                 ttatggatgTAACAGCTGTTGGCGATCCGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCTACAGCGGAATGTCTTCGGGGTGGTGCGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAccc                                                                                                                                                                           <  4:23383/267‑1 (MQ=255)
                                           ccGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCGCCAGCGGCATCTCTTCGGGTTTGTGCGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCg                                                                                                                                                 <  2:16381/275‑1 (MQ=255)
                                                                                                                             cccTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGt                                                                                                                                                                                              <  6:215598/149‑1 (MQ=255)
                                                                                                                              ccTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTg                                                                                                                                                                                             >  5:637924/1‑149 (MQ=255)
                                                                                                                              ccTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTg                                                                                                                                                                                             >  8:304930/1‑149 (MQ=255)
                                                                                                                              ccTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTg                                                                                                                                                                                             >  7:1032369/1‑149 (MQ=255)
                                                                                                                               cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACTATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                                                            <  8:13334/149‑1 (MQ=255)
                                                                                                                               cTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                                                            >  5:1245450/1‑149 (MQ=255)
                                                                                                                                tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                                                            <  5:447704/148‑1 (MQ=255)
                                                                                                                                tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                                                            >  6:165351/1‑148 (MQ=255)
                                                                                                                                tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGa                                                                                                                                                                                            <  5:165351/148‑1 (MQ=255)
                                                                                                                                tGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAg                                                                                                                                                                                           >  7:1191852/1‑149 (MQ=255)
                                                                                                                                 gAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAgg                                                                                                                                                                                          >  7:795362/1‑149 (MQ=255)
                                                                                                                                      gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                                                                    <  6:813282/149‑1 (MQ=255)
                                                                                                                                      gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                                                                    >  5:562965/1‑149 (MQ=255)
                                                                                                                                      gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                                                                    <  5:500343/149‑1 (MQ=255)
                                                                                                                                      gTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGAt                                                                                                                                                                                    >  8:1173538/1‑149 (MQ=255)
                                                                                                                                        tAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATCAGCCTCACGGGAGGCTgtatgt                                                                                                                                                                                  <  5:874497/149‑6 (MQ=255)
                                                                                                                                          aTTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTCATTAACGACATACAGCCTCACGGGAGGCTGTATGTc                                                                                                                                                                                 <  8:1379087/149‑1 (MQ=255)
                                                                                                                                            tCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCaaa                                                                                                                                                                              <  8:69183/149‑1 (MQ=255)
                                                                                                                                               gCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAccc                                                                                                                                                                           <  7:606312/149‑1 (MQ=255)
                                                                                                                                                ccAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAAc                                                                                                                                                                     >  4:70504/1‑154 (MQ=255)
                                                                                                                                                    aCCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAATTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACGTACAGCCTCCCGTGAGGCTGATGTCAAACCCaaaaa                                                                                                                                                                      <  6:1021435/149‑1 (MQ=255)
                                                                                                                                                    aCCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCaaaaa                                                                                                                                                                      <  6:1050744/149‑1 (MQ=255)
                                                                                                                                                      cAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACt                                                                                                                                                                    <  8:571343/149‑1 (MQ=255)
                                                                                                                                                       aGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTg                                                                                                                                                                   >  8:1276404/1‑149 (MQ=255)
                                                                                                                                                        gCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGc                                                                                                                                                                  <  7:1138991/149‑1 (MQ=255)
                                                                                                                                                          aCCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGACACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCAc                                                                                                                                                                >  7:831760/1‑149 (MQ=255)
                                                                                                                                                                   cacaATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTc                                                                                                                                                       <  5:1088498/149‑1 (MQ=255)
                                                                                                                                                                     caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTccc                                                                                                                                                     <  7:397562/149‑1 (MQ=255)
                                                                                                                                                                      aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAc                                                                                                                                                                             <  7:929686/124‑1 (MQ=255)
                                                                                                                                                                      aaTGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAAc                                                                                                                                                                             >  8:929686/1‑124 (MQ=255)
                                                                                                                                                                          gTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGc                                                                                                                                                <  5:1466576/149‑1 (MQ=255)
                                                                                                                                                                               gTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                                                                           >  7:1079983/1‑149 (MQ=255)
                                                                                                                                                                                tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                                                          <  8:1160367/149‑1 (MQ=255)
                                                                                                                                                                                 gATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTg                                                                                                                                         >  6:1150922/1‑149 (MQ=255)
                                                                                                                                                                                  aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                        <  7:1276404/149‑1 (MQ=255)
                                                                                                                                                                                  aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                        <  8:803256/149‑1 (MQ=255)
                                                                                                                                                                                  aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                        <  5:1165193/149‑1 (MQ=255)
                                                                                                                                                                                  aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                        <  6:136385/149‑1 (MQ=255)
                                                                                                                                                                                  aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                        <  8:221108/149‑1 (MQ=255)
                                                                                                                                                                                   tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                                                                       <  8:1194532/149‑1 (MQ=255)
                                                                                                                                                                                   tAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                                                                       <  6:1245450/149‑1 (MQ=255)
                                                                                                                                                                                     aTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                     <  6:1080125/149‑1 (MQ=255)
                                                                                                                                                                                          ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAggg                                                                                                                                >  8:593800/1‑149 (MQ=255)
                                                                                                                                                                                               gCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                                                                           >  7:69142/1‑149 (MQ=255)
                                                                                                                                                                                                 aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGga                                                                                                                         >  6:1455313/1‑149 (MQ=255)
                                                                                                                                                                                                   gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgaga                                                                                                                       <  6:427308/149‑1 (MQ=255)
                                                                                                                                                                                                   gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgaga                                                                                                                       <  8:966453/149‑1 (MQ=255)
                                                                                                                                                                                                    tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                   >  7:1427710/1‑136 (MQ=255)
                                                                                                                                                                                                    tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                   <  8:1427710/136‑1 (MQ=255)
                                                                                                                                                                                                    tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                      >  7:42219/1‑149 (MQ=255)
                                                                                                                                                                                                    tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                      <  7:369248/149‑1 (MQ=255)
                                                                                                                                                                                                     gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                                                                     <  7:1173538/149‑1 (MQ=255)
                                                                                                                                                                                                         cGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaa                                                                                                                 >  6:1258221/1‑149 (MQ=255)
                                                                                                                                                                                                                tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                                                          >  6:24345/1‑149 (MQ=255)
                                                                                                                                                                                                                    cTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                                                                       >  5:860545/1‑116 (MQ=255)
                                                                                                                                                                                                                    cTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                                                                           <  6:726578/128‑1 (MQ=255)
                                                                                                                                                                                                                    cTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                                                                           >  5:726578/1‑128 (MQ=255)
                                                                                                                                                                                                                       cAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAt                                                                                                   >  7:189545/1‑149 (MQ=255)
                                                                                                                                                                                                                         aTGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACAGACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAttt                                                                                                 >  8:350098/1‑149 (MQ=255)
                                                                                                                                                                                                                          tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCa                                                                                                                                                                          >  1:53292/1‑75 (MQ=255)
                                                                                                                                                                                                                           gCATTTTGGTGATTTGCAAACAGGGCACCTTAATTAACAACATACACCCCCCCGAGAGGCTGATGTAAACCCCAAAAACTGCACCGGACGGTCCCCCCGCCCCTTTGGGGAGAGGGTTGGGGAGAGGGGAAAACCCGCACCCTATTTTc                                                                                               <  5:257833/149‑1 (MQ=38)
                                                                                                                                                                                                                           gCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTc                                                                                               >  7:542915/1‑149 (MQ=255)
                                                                                                                                                                                                                              tatTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCaaaaa                                                                                                                                                                      <  3:53292/75‑1 (MQ=255)
                                                                                                                                                                                                                               atTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACGGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                                                           <  6:938131/149‑1 (MQ=255)
                                                                                                                                                                                                                                ttGGTGATTTGCATACGAGGCACCTTAATTAACGACATGCAGCCTCCCGTGAGGCTGAGGTCAAACGCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGATGGTTAGGGAGAGGGGAAAACCCGCACCCTATATTCAGATc                                                                                          >  7:907595/1‑149 (MQ=39)
                                                                                                                                                                                                                                 tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                   <  4:179439/107‑1 (MQ=255)
                                                                                                                                                                                                                                 tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa                                                                                         <  8:542915/149‑1 (MQ=255)
                                                                                                                                                                                                                                           cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc                                                                               >  7:1390810/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aaaCAAGACACATAAATTAACGACATACACCCGCCCGTGAGGCTAATGTCAAACCCAAAACCTGCACCGGACGGTCCCCTCGCCCCTTGGGGCAGAGGGTTAGGGAGAGGGGAAAACCCGCAGCCTATTTTCAGATCAGTTATTTCGCa                                                                              <  7:1222576/147‑1 (MQ=255)
                                                                                                                                                                                                                                            aTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                                                              >  7:1216827/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                                                              >  5:1367211/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCTTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                          <  7:589577/149‑1 (MQ=255)
                                                                                                                                                                                                                                                aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                          >  6:992302/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                          >  6:1048957/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aaGGAACCTTAATCAGCGACATACAGCCCCCCGGGGGGCTGATGGAAAACCCAAAAACTGCACGGGTCGGTCCCCTCGCCCCTTTGGGGAGAGGGTTGGGGGGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                          <  7:343736/149‑1 (MQ=37)
                                                                                                                                                                                                                                                  ggCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                      >  2:33593/1‑103 (MQ=255)
                                                                                                                                                                                                                                                  ggCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                                        >  5:1417956/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  ggCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt  >  2:67559/1‑219 (MQ=255)
                                                                                                                                                                                                                                                   gCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAatat                                                                       >  5:285928/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                                                                  >  6:632255/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           attaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                                                               >  6:1000785/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            ttaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                                                              >  5:312073/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt                                                         >  5:265015/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   cATACGGCCTCCCTGGAGGCTGATGTCAAACCCAAAACCTGCACCGGACGGTCCCCTCGCCCCTTGGGGGAGAGGGTAAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGc                                                       <  7:66920/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                    catcAGCCTCACGGGAGGCTGTATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGc                                                       >  6:1263407/4‑149 (MQ=39)
                                                                                                                                                                                                                                                                    aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa                                                      >  6:684690/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCGCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTGAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                     >  5:1208777/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                     >  6:568705/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                     >  6:327539/3‑149 (MQ=255)
                                                                                                                                                                                                                                                                          ccTCCCGTGAGGCTGTTGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCt                                                >  8:493336/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           ccccggTGAGGCTGATGAAAACCCCAACACCCGCACCGGAGGGTCCCGTCGCCCTTTTGGGGAGGGGTTTAGGGAGACGGGAAACCCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTg                                               <  6:538621/144‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca                                                                 <  8:351234/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTgg                                              >  7:503939/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                            tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTgg                                              >  6:461208/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                            tCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTgg                                              <  6:285928/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             accttGAGGCTGATGTCAAACCCAAAAACTGCCCCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGa                                             <  8:1446734/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                            
ACTGATATTCCTGCCCCTGATAGCGGTAACAGCCGTTGGCGATCCGGTTCGGCCACGGAATAAGCACTTTACCCAGATGCGCCAGCGGCATCTCTTCGGGTTTGTGCGGGATGACTAAATGACAACCCTGAAAGGTTAATTCAGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAT  >  NC_000913/4070140‑4070600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: